Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae

Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.Os antígenos metabólicos de Ascaris suum foram obtidos de larvas de segundo e de terceiro estágio (precoce) cultivadas in vitro em meio mínimo essencial de Eeagle com suplemento proteico. Os antígenos metabólicos coletados depois de 12 a 16 dias das culturas in vitro foram eluidos em Bio-Gel Al 5. Três picos de eluição foram identificados, dializados, liofilizados e injetados em camundongos, com alginato de sódio a 4%. O pico II da eluição de duas preparações dos antígenos metabólicos protegeram os camundongos da infecção com 10.000 ovos infectivos de A. suumEscola Paulista de Medicina Centro de Pesquisas em Fisiologia e Imunologia de Helmintos Departamento de Microbiologia e ParasitologiaUniversity of New Mexico Department of BiologyUNIFESP, EPM, Centro de Pesquisas em Fisiologia e Imunologia de Helmintos Depto. de Microbiologia e ParasitologiaSciEL

Globular Cluster Populations in Four Early-Type Poststarburst Galaxies

We present a study of the globular cluster systems of four early-type poststarburst galaxies using deep g and I-band images from the ACS camera aboard the Hubble Space Telescope (HST). All the galaxies feature shells distributed around their main bodies and are thus likely merger remnants. The color distribution of the globular clusters in all four galaxies shows a broad peak centered on g-I ~ 1.4, while PGC 6240 and PGC 42871 show a significant number of globular clusters with g-I ~ 1.0. The latter globular clusters are interpreted as being of age ~ 500 Myr and likely having been formed in the merger. The color of the redder peak is consistent with that expected for an old metal-poor population that is very commonly found around normal galaxies. However, all galaxies except PGC 10922 contain several globular clusters that are significantly brighter than the maximum luminosity expected of a single old metal-poor population. To test for multiple-age populations of overlapping g-I color, we model the luminosity functions of the globular clusters as composites of an old metal-poor subpopulation with a range of plausible specific frequencies and an intermediate-age subpopulation of solar metallicity. We find that three of the four sample galaxies show evidence for the presence of an intermediate-age (~ 1 Gyr) globular cluster population, in addition to the old metal-poor GC population seen in normal early-type galaxies. None of the galaxies show a significant population of clusters consistent with an old, metal-rich red cluster population that is typically seen in early-type galaxies.Comment: 41 pages, 13 figures, accepted for publication in AJ. Some figues have been downgraded to reduce their size

Age-related macular degeneration: choroidal ischaemia?

Aim: Our aim is to use ultrasound to non-invasively detect differences in choroidal microarchitecture possibly related to ischaemia among normal eyes and those with wet and dry age-related macular degeneration (AMD). Design Prospective case series of subjects with dry AMD, wet AMD and age-matched controls. Methods: Digitized 20 MHz B-scan radiofrequency ultrasound data of the region of the macula were segmented to extract the signal from the retina and choroid. This signal was processed by a wavelet transform, and statistical modelling was applied to the wavelet coefficients to examine differences among dry, wet and non-AMD eyes. Receiver operating characteristic (ROC) analysis was used to evaluate a multivariate classifier. Results: In the 69 eyes of 52 patients, 18 did not have AMD, 23 had dry AMD and 28 had wet AMD. Multivariate models showed statistically significant differences between groups. Multiclass ROC analysis of the best model showed an excellent volume-under-curve of 0.892±0.17. The classifier is consistent with ischaemia in dry AMD. Conclusions: Wavelet augmented ultrasound is sensitive to the organisational elements of choroidal microarchitecture relating to scatter and fluid tissue boundaries such as seen in ischaemia and inflammation, allowing statistically significant differentiation of dry, wet and non-AMD eyes. This study further supports the association of ischaemia with dry AMD and provides a rationale for treating dry AMD with pharmacological agents to increase choroidal perfusion

The utility of MRI in predicting radiographic erosions in the metatarsophalangeal joints of the rheumatoid foot: a prospective longitudinal cohort study

INTRODUCTION: Magnetic resonance imaging (MRI) may reveal rheumatoid arthritis (RA) changes in the feet when hands are normal. The purpose of this study was to determine the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of a metatarsophalangeal (MTP) erosion on MRI to predict a subsequent radiographic erosion in the same joint. Similar analyses were performed for bone marrow edema, predicting a subsequent MRI erosion. Descriptive results of other lesions are reported. METHODS: Fifty patients with RA of less than 5 years' duration who were rheumatoid factor-positive and/or anti-cyclic citrullinated peptide-positive were recruited. Patients on anti-tumor necrosis factor (TNF) therapy were excluded. Anti-TNF therapy could begin after enrollment. MRI and radiographs of the 3rd, 4th, and 5th MTP joints bilaterally were taken at baseline and at 6, 12, and 24 months. Clinical data were collected. RESULTS: Fifty patients were recruited; 46 patients had suitable data. Results for MRI erosions predicting subsequent radiographic erosions for 6, 12, and 24 months, respectively, were as follows: sensitivity 0.75, 0.60, 0.75; specificity 0.93, 0.94, 0.94; PPV 0.086, 0.10, 0.17; NPV 0.998, 0.995, 0.995. Results for MRI bone marrow edema predicting MRI erosions at 6 and 12 months, respectively, revealed sensitivity 0.50, 0.67; specificity 0.97, 0.97; PPV 0.25, 0.50; NPV 0.99, 0.99. Synovitis was the most common finding and, when present in isolation, resolved on 67.3% of subsequent studies. MRI erosions persisted on subsequent studies with one exception. Forty-six percent of the cohort was on anti-TNF therapy after study inception. CONCLUSIONS: The PPV of MRI erosions to predict subsequent radiographic erosions was low. Similarly, the PPV of bone marrow edema to predict a later MRI erosion was low. Alternatively, the NPV of the absence of an MRI erosion or bone marrow edema predicts that a later radiographic erosion or MRI erosion will likely not develop. Anti-TNF therapies may have resulted in the lower-than-anticipated PPVs. MRI descriptions of bone edema may represent a more critical time to treat in order to avoid damage, whereas an MRI erosion represents more permanent damage. This study suggests that imaging modalities more sensitive than radiographs are necessary to monitor disease in the biologic era

Measurement of choroidal perfusion and thickness following systemic sildenafil (Viagra)

Purpose: To demonstrate anatomic and physiologic changes in the human choroid following systemic sildenafil citrate (Viagra) using enhanced depth imaging spectral domain–optical coherence tomography (EDI-OCT) and swept-scan high-frequency digital ultrasound. Methods: Seven healthy male subjects (mean age 32.7 years) were evaluated at baseline and 2 hr after ingesting 50 mg of sildenafil. Swept-scan high-frequency digital ultrasound and EDI-OCT were utilized to measure choroidal perfusion and thickness, respectively. Results were read by masked observers. The Wilcoxon signed-rank test and t-test were used to analyse differences in choroidal flow and thickness at baseline and 2 hr after ingestion of sildenafil. Results: Two hours following sildenafil, increased choroidal perfusion was observed in 11 of 12 eyes measured by swept-scan high-frequency digital ultrasound. The mean increase was 3.46 (±2.00) times baseline with a range of 0.47–7.80 times baseline (p = 0.004). Increased choroidal thickness was observed in 12 of 12 eyes measured with EDI-OCT. The average choroidal thickness increased by 11.6% temporal to the fovea, 9.3% nasal to the fovea and 10.7% underneath the fovea (p < 0.001 for all values). Conclusions: Choroidal perfusion and thickness both increase in response to systemic sildenafil. These changes could secondarily affect retinal function, explain previously reported clinical symptoms and potentially be a useful adjunct for treatment of ocular diseases that would benefit from increased choroidal blood flow

Population Parameters of Intermediate-Age Star Clusters in the Large Magellanic Cloud. I. NGC 1846 and its Wide Main Sequence Turnoff

The Advanced Camera for Surveys on board the Hubble Space Telescope has been used to obtain deep, high-resolution images of the intermediate-age star cluster NGC 1846 in the Large Magellanic Cloud. We present new color-magnitude diagrams (CMDs) based on F435W, F555W, and F814W imaging. We test the previously observed broad main sequence turnoff region for "contamination" by field stars and (evolved) binary star systems. We find that while these impact the number of objects in this region, none can fully account for the large color spread. Our results therefore solidify the recent finding that stars in the main sequence turnoff region of this cluster have a large spread in color which is unrelated to measurement errors or contamination by field stars, and likely due to a ~300 Myr range in the ages of cluster stars. An unbiased estimate of the stellar density distribution across the main sequence turnoff region shows that the spread is fairly continuous rather than strongly bimodal as suggested previously. We fit the CMDs with several different sets of theoretical isochrones, and determine systematic uncertainties for population parameters when derived using any one set of isochrones. We note a degeneracy between age and [alpha/Fe], which can be lifted by matching the shape (curvature) of the full red giant branch in the CMD. We find that stars in the upper part of the main sequence turnoff region are more centrally concentrated than those in any other region of the CMD, including more massive red giant branch and asymptotic giant branch stars. We consider several possible formation scenarios which account for the unusual features observed in the CMD of NGC 1846.Comment: 17 pages, 17 figures, in emulateapj format. Figures 1 and 2 have been downgraded in resolution in this version. Accepted for publication in The Astronomical Journa

Chandra Observations of the QSO Pair Q2345+007: Binary Quasar or Massive Dark Lens?

The components of the wide (7.3") separation quasar pair Q2345+007A,B (z=2.15) have the most strikingly similar optical spectra seen to date (Steidel & Sargent 1991) yet no detected lensing mass, making this system the best candidate known for a massive (1e14 Msun) dark matter lens system. Here we present results from a 65ksec Chandra observation designed to investigate whether it is a binary quasar or a gravitational lens. We find no X-ray evidence for a lensing cluster to a (0.5-2keV) flux limit of 2e-15 cgs, which is consistent with lensing only for a reduced baryon fraction. Using the Chandra X-ray observations of the quasars themselves, together with new and published optical measurements, we use the observed emission properties of the quasars for further tests between the lens and binary hypotheses. Assuming similar line-of-sight absorption to the images, we find that their X-ray continuum slopes are inconsistent (Gamma_A=2.30 and Gamma_B=0.83) as are their X-ray to optical flux ratios. The probability that B suffers absorption sufficient to account for these spectral differences is negligible. We present new optical evidence that the flux ratio of the pair is variable, so the time-delay in a lens scenario could cause some of the discrepancies. However, adequately large variations in overall spectral energy distribution are rare in individual QSOs. All new evidence here weighs strongly toward the binary interpretation. Q2345+007 thus may represent the highest redshift example known of interaction-triggered but as-yet unmerged luminous AGN.Comment: 15 pages, Latex, emulateapj style, including 3 tables and 5 figures. Accepted Feb 1, 2002 for publication in ApJ Main Journal. See also http://hea-www.harvard.edu/~pgreen/Papers.htm

SN2012ab: A Peculiar Type IIn Supernova with Aspherical Circumstellar Material

We present photometry, spectra, and spectropolarimetry of supernova (SN) 2012ab, mostly obtained over the course of $\sim 300$ days after discovery. SN 2012ab was a Type IIn (SN IIn) event discovered near the nucleus of spiral galaxy 2MASXJ12224762+0536247. While its light curve resembles that of SN 1998S, its spectral evolution does not. We see indications of CSM interaction in the strong intermediate-width emission features, the high luminosity (peak at absolute magnitude $M=-19.5$), and the lack of broad absorption features in the spectrum. The H$\alpha$ emission undergoes a peculiar transition. At early times it shows a broad blue emission wing out to $-14{,}000$ km $\mathrm{s^{-1}}$ and a truncated red wing. Then at late times ($>$ 100$\,$days) it shows a truncated blue wing and a very broad red emission wing out to roughly $+20{,}000$ km $\mathrm{s^{-1}}$. This late-time broad red wing probably arises in the reverse shock. Spectra also show an asymmetric intermediate-width H$\alpha$ component with stronger emission on the red side at late times. The evolution of the asymmetric profiles requires a density structure in the distant CSM that is highly aspherical. Our spectropolarimetric data also suggest asphericity with a strong continuum polarization of $\sim 1-3$% and depolarization in the H$\alpha$ line, indicating asphericity in the CSM at a level comparable to that in other SNe IIn. We estimate a mass-loss rate of $\dot{M} = 0.050\, {\rm M}_{\odot}\,\mathrm{yr^{-1}}$ for $v_{\rm pre} = 100$$\,$km$\,$$\mathrm{s^{-1}}$ extending back at least 75$\,$yr prior to the SN. The strong departure from axisymmetry in the CSM of SN 2012ab may suggest that the progenitor was an eccentric binary system undergoing eruptive mass loss.Comment: 18 pages, 12 figure

Neuroimaging Evidence of Major Morpho-Anatomical and Functional Abnormalities in the BTBR T+TF/J Mouse Model of Autism

BTBR T+tf/J (BTBR) mice display prominent behavioural deficits analogous to the defining symptoms of autism, a feature that has prompted a widespread use of the model in preclinical autism research. Because neuro-behavioural traits are described with respect to reference populations, multiple investigators have examined and described the behaviour of BTBR mice against that exhibited by C57BL/6J (B6), a mouse line characterised by high sociability and low self-grooming. In an attempt to probe the translational relevance of this comparison for autism research, we used Magnetic Resonance Imaging (MRI) to map in both strain multiple morpho-anatomical and functional neuroimaging readouts that have been extensively used in patient populations. Diffusion tensor tractography confirmed previous reports of callosal agenesis and lack of hippocampal commissure in BTBR mice, and revealed a concomitant rostro-caudal reorganisation of major cortical white matter bundles. Intact inter-hemispheric tracts were found in the anterior commissure, ventro-medial thalamus, and in a strain-specific white matter formation located above the third ventricle. BTBR also exhibited decreased fronto-cortical, occipital and thalamic gray matter volume and widespread reductions in cortical thickness with respect to control B6 mice. Foci of increased gray matter volume and thickness were observed in the medial prefrontal and insular cortex. Mapping of resting-state brain activity using cerebral blood volume weighted fMRI revealed reduced cortico-thalamic function together with foci of increased activity in the hypothalamus and dorsal hippocampus of BTBR mice. Collectively, our results show pronounced functional and structural abnormalities in the brain of BTBR mice with respect to control B6 mice. The large and widespread white and gray matter abnormalities observed do not appear to be representative of the neuroanatomical alterations typically observed in autistic patients. The presence of reduced fronto-cortical metabolism is of potential translational relevance, as this feature recapitulates previously-reported clinical observations

The Quantity Theory of Money is Valid. The New Keynesians are Wrong!

We test the quantity theory of money (QTM) using a novel approach and a large new sample. We do not follow the usual approach of first differentiating the logarithm of the Cambridge equation to obtain an equation relating the growth rate of real GDP, the growth rate of money and inflation. These variables must then again be ‘integrated’ by averaging in order to obtain stable relationships. Instead we suggest a much simpler procedure for testing directly the stability of the coefficient of the Cambridge equation. For 125 countries and post-war data we find the coefficient to be surprisingly stable. We do not select for high inflation episodes as was done in most empirical studies; inflation rates do not even appear in our data set. Much work supporting the QTM has been done by economic historians and at the University of Chicago by Milton Friedman and his associates. The QTM was a foundation stone of the monetarist revolution. Subsequently belief in it waned. The currently dominant New Keynesian School, implicitly or explicitly denies the validity of the QTM. We survey this history and argue that the QTM is valid and New Keynesians are wrong