Physical drivers facilitating a toxigenic cyanobacterial bloom in a major Great Lakes tributary

The Maumee River is the primary source for nutrients fueling seasonal Microcystis-dominated blooms in western Lake Erie\u27s open waters though such blooms in the river are infrequent. The river also serves as source water for multiple public water systems and a large food services facility in northwest Ohio. On 20 September 2017, an unprecedented bloom was reported in the Maumee River estuary within the Toledo metropolitan area, which triggered a recreational water advisory. Here we (1) explore physical drivers likely contributing to the bloom\u27s occurrence, and (2) describe the toxin concentration and bacterioplankton taxonomic composition. A historical analysis using 10-years of seasonal river discharge, water level, and local wind data identified two instances when high-retention conditions occurred over ≥ 10 d in the Maumee River estuary: in 2016 and during the 2017 bloom. Observation by remote sensing imagery supported the advection of cyanobacterial cells into the estuary from the lake during 2017 and the lack of an estuary bloom in 2016 due to a weak cyanobacterial bloom in the lake. A rapid-response survey during the 2017 bloom determined levels of the cyanotoxins, specifically microcystins, in excess of recreational contact limits at sites within the lower 20 km of the river while amplicon sequencing found these sites were dominated by Microcystis. These results highlight the need to broaden our understanding of physical drivers of cyanobacterial blooms within the interface between riverine and lacustrine systems, particularly as such blooms are expected to become more prominent in response to a changing climate

Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States)

This study examined diel shifts in metabolic functions of spp. during a 48-h Lagrangian survey of a toxin-producing cyanobacterial bloom in western Lake Erie in the aftermath of the 2014 Toledo Water Crisis. Transcripts mapped to the genomes of recently sequenced lower Great Lakes isolates showed distinct patterns of gene expression between samples collected across day (10:00 h, 16:00 h) and night (22:00 h, 04:00 h). Daytime transcripts were enriched in functions related to Photosystem II (e.g., ), nitrogen and phosphate acquisition, cell division (), heat shock response (, ), and uptake of inorganic carbon (, ). Genes transcribed during nighttime included those involved in phycobilisome protein synthesis and Photosystem I core subunits. Hierarchical clustering and principal component analysis (PCA) showed a tightly clustered group of nighttime expressed genes, whereas daytime transcripts were separated from each other over the 48-h duration. Lack of uniform clustering within the daytime transcripts suggested that the partitioning of gene expression in is dependent on both circadian regulation and physicochemical changes within the environment

Crop Updates 2001 - Oilseeds

ABSTRACT This session covers twenty five papers from different authors: FORWARD, Mervyn McDougall, CHAIRMAN, PULSES AND OILSEEDS PARTNERSHIP GROUP PLENARY 1. Implications of the ‘green-bridge’ for viral and fungal disease carry-over between seasons, Debbie Thackray, Agriculture Western Australia and Centre for Legumes in Mediterranean Agriculture 2. Insect pest development in WA via the ‘green-bridge’, Kevin Walden, Agriculture Western Australia VARIETIES 3. Performance of new canola varieties in AGWEST variety trials, G. Walton, Crop Improvement Institute, Agriculture Western Australia 4. New herbicide tolerant varieties in WA, Kevin Morthorpe, Stephen Addenbrooke, Pioneer Hi-Bred Australia P/L 5. IT v’s TT – Head to head, Paul Carmody, Centre for Cropping Systems, Agriculture Western Australia ESTABLISHMENT 6. Effect of stubble, seeding technique and seed size on crop establishment and yield of canola, Rafiul Alam, Glen Riethmuller and Greg Hamilton, Agriculture Western Australia 7. Canola establishment survey 2000, Rafiul Alam, Paul Carmody, Greg Hamilton and Adrian Cox, Agriculture Western Australia 8. Tramline farming for more canola, Paul Blackwell, Agriculture Western Australia NUTRITION 9. Comparing the phosphorus requirement of canola and wheat in WA, M.D.A. Bolland and M.J. Baker, Agriculture Western Australia 10. Will a rainy summer affect nitrogen requirement: Tailoring your fertiliser decisions using the new nitrogen calculator, A.J. Diggle, Agriculture Western Australia 11. Canola – More response to lime, Chris Gazeyand Paul Carmody, Centre for Cropping Systems, Agriculture Western Australia AGRONOMY 12. Hormone manipulation of canola development, Paul Carmody and Graham Walton, Agriculture Western Australia 13. Yield penalties with delayed sewing of canola, Imma Farre, CSIRO Plant Industry, Michael J. Robertson, CSIRO Sustainable Ecosystems, Graham H. Walton, Agriculture Western Australia, Senthold Asseng, CSIRO Plant Industry 14. Dry matter and oil accumulation in developing seeds of canola varieties at different sowing dates, Ping Si1, David Turner1 and David Harris2 , 1Plant Sciences, Faculty of Agriculture, The University of Western Australia, 2Chemistry Centre of Western Australia 13. Simulating oil concentrations in canola – virtually just the beginning, David Turner1 and Imma Farré2, 1Plant Sciences, Faculty of Agriculture, The University of Western Australia, 2CSIRO Plant Industry, Centre for Mediterranean Agricultural Research PESTS AND DISEASES 14. Further evidence that canola crops are resilient to damage by aphids, Françoise Berlandier and Christiaan Valentine, Entomology, Agriculture Western Australia 15. Management of Diamondback moth (DBM) in canola, David Cook, Peter Mangano, David Cousins, Françoise Berlandier, and Darryl Hardie, Crop Improvement Institute,Agriculture Western Australia 16. Effect of time of sowing in conjunction with fungicides on blackleg and yield of canola, Ravjit Khangura and Martin Barbetti, Agriculture Western Australia 17. Further developments in forecasting aphid and virus risk in canola, Debbie Thackray, Jenny Hawkes and Roger Jones, Agriculture Western Australia and Centre for Legumes in Mediterranean Agriculture 18. Efficiency of selected insecticides for the use on Diamondback Moth in canola, Kevin Walden, Agriculture Western Australia 19. Impact® applied ‘in furrow’ controls blackleg in canola, Cameron Weeks and Erin Hasson, Mingenew-Irwin Group Inc. 20. Effect of time of sowing and Impact® on canola yield, Esperance, Dave Eksteen, Agriculture Western Australia 21. Australian Plague Locust Campaign 2000, Kevin Walden, Agriculture Western Australia WEED CONTROL 22. New herbicide options for canola, John Moore and Paul Matson, Agriculture Western Australia HARVESTING 23. Effects of time of swathing and desiccant application on the seed yield and oil content of canola, Carla Thomas and Lionel Martin, Muresk Institute of Agriculture, Curtin University of Technology DECISION SUPPORT AND ADOPTION 24. Using canola monitoring groups to understand factors affecting canola production in Esperance, Dave Eksteen, Agriculture Western Australia 25. Nitrogen and canola, Dave Eksteen, Agriculture Western Australi

Ready or not? Expectations of faculty and medical students for clinical skills preparation for clerkships

Background: Preclerkship clinical-skills training has received increasing attention as a foundational preparation for clerkships. Expectations among medical students and faculty regarding the clinical skills and level of skill mastery needed for starting clerkships are unknown. Medical students, faculty teaching in the preclinical setting, and clinical clerkship faculty may have differing expectations of students entering clerkships. If students' expectations differ from faculty expectations, students may experience anxiety. Alternately, congruent expectations among students and faculty may facilitate integrated and seamless student transitions to clerkships. Aims: To assess the congruence of expectations among preclerkship faculty, clerkship faculty, and medical students for the clinical skills and appropriate level of clinical-skills preparation needed to begin clerkships. Methods: Investigators surveyed preclinical faculty, clerkship faculty, and medical students early in their basic clerkships at a North American medical school that focuses on preclerkship clinical-skills development. Survey questions assessed expectations for the appropriate level of preparation in basic and advanced clinical skills for students entering clerkships. Results: Preclinical faculty and students had higher expectations than clerkship faculty for degree of preparation in most basic skills. Students had higher expectations than both faculty groups for advanced skills preparation. Conclusions: Preclinical faculty, clerkship faculty, and medical students appear to have different expectations of clinical-skills training needed for clerkships. As American medical schools increasingly introduce clinical-skills training prior to clerkships, more attention to alignment, communication, and integration between preclinical and clerkship faculty will be important to establish common curricular agendas and increase integration of student learning. Clarification of skills expectations may also alleviate student anxiety about clerkships and enhance their learning

Terminology for chain polymerization (IUPAC Recommendations 2021)

Chain polymerizations are defined as chain reactions where the propagation steps occur by reaction between monomer(s) and active site(s) on the polymer chains with regeneration of the active site(s) at each step. Many forms of chain polymerization can be distinguished according to the mechanism of the propagation step (e.g., cyclopolymerization – when rings are formed, condensative chain polymerization – when propagation is a condensation reaction, group-transfer polymerization, polyinsertion, ring-opening polymerization – when rings are opened), whether they involve a termination step or not (e.g., living polymerization – when termination is absent, reversible-deactivation polymerization), whether a transfer step is involved (e.g., degenerative-transfer polymerization), and the type of chain carrier or active site (e.g., radical, ion, electrophile, nucleophile, coordination complex). The objective of this document is to provide a language for describing chain polymerizations that is both readily understandable and self-consistent, and which covers recent developments in this rapidly evolving field

Custom Integrated Circuits

Contains reports on seven research projects.U.S. Air Force - Office of Scientific Research (Contract F49620-84-C-0004)National Science Foundation (Grant ECS81-18160)Defense Advanced Research Projects Agency (Contract NOO14-80-C-0622)National Science Foundation (Grant ECS83-10941

Terminology for chain polymerization (IUPAC Recommendations 2021)

Chain polymerizations are defined as chain reactions where the propagation steps occur by reaction between monomer(s) and active site(s) on the polymer chains with regeneration of the active site(s) at each step. Many forms of chain polymerization can be distinguished according to the mechanism of the propagation step (e.g., cyclopolymerization – when rings are formed, condensative chain polymerization – when propagation is a condensation reaction, group-transfer polymerization, polyinsertion, ring-opening polymerization – when rings are opened), whether they involve a termination step or not (e.g., living polymerization – when termination is absent, reversible-deactivation polymerization), whether a transfer step is involved (e.g., degenerative-transfer polymerization), and the type of chain carrier or active site (e.g., radical, ion, electrophile, nucleophile, coordination complex). The objective of this document is to provide a language for describing chain polymerizations that is both readily understandable and self-consistent, and which covers recent developments in this rapidly evolving field

Terminology for chain polymerization (IUPAC Recommendations 2021)

Chain polymerizations are defined as chain reactions where the propagation steps occur by reaction between monomer(s) and active site(s) on the polymer chains with regeneration of the active site(s) at each step. Many forms of chain polymerization can be distinguished according to the mechanism of the propagation step (e.g., cyclopolymerization – when rings are formed, condensative chain polymerization – when propagation is a condensation reaction, group-transfer polymerization, polyinsertion, ring-opening polymerization – when rings are opened), whether they involve a termination step or not (e.g., living polymerization – when termination is absent, reversible-deactivation polymerization), whether a transfer step is involved (e.g., degenerative-transfer polymerization), and the type of chain carrier or active site (e.g., radical, ion, electrophile, nucleophile, coordination complex). The objective of this document is to provide a language for describing chain polymerizations that is both readily understandable and self-consistent, and which covers recent developments in this rapidly evolving field

Custom Integrated Circuits

Contains reports on six research projects.U.S. Air Force - Office of Scientific Research (Contract F49620-84-C-0004)Analog Devices, Inc.Defense Advanced Research Projects Agency (Contract N00014-80-C-0622)National Science Foundation (Grant ECS83-10941

Differentiating Salmonid Migratory Ecotypes Through Stable Isotope Analysis of Collagen: Archaeological and Ecological Applications

The ability to distinguish between different migratory behaviours (e.g., anadromy and potamodromy) in fish can provide important insights into the ecology, evolution, and conservation of many aquatic species. We present a simple stable carbon isotope (δ13C) approach for distinguishing between sockeye (anadromous ocean migrants) and kokanee (potamodromous freshwater residents), two migratory ecotypes of Oncorhynchus nerka (Salmonidae) that is applicable throughout most of their range across coastal regions of the North Pacific Ocean. Analyses of kokanee (n = 239) and sockeye (n = 417) from 87 sites spanning the North Pacific (Russia to California) show that anadromous and potamodromous ecotypes are broadly distinguishable on the basis of the δ13C values of their scale and bone collagen. We present three case studies demonstrating how this approach can address questions in archaeology, archival, and conservation research. Relative to conventional methods for determining migratory status, which typically apply chemical analyses to otoliths or involve genetic analyses of tissues, the δ13C approach outlined here has the benefit of being non-lethal (when applied to scales), cost-effective, widely available commercially, and should be much more broadly accessible for addressing archaeological questions since the recovery of otoliths at archaeological sites is rare