Perceptions and Attitudes to Waste Disposal: An Assessment of Waste Disposal Behaviors in the Tamale Metropolis

The study was done in the Northern Region of Ghana (Tamale metropolis) in 2012. Primary data on waste collection and management were collected at the household level (210 households) using questionnaires based on the Theory of Reasoned Action (TORA) construct. Secondary data was also collected from records of Zoom-lion Ghana Limited as well as the Tamale Municipal Waste Management Department. The TORA model was employed to evaluate how perceptions and attitudes of households influenced disposal of solid waste and willingness to pay (WTP). Overall, attitude of households had strong influence on the intention to use collection bins in future. On the other hand, social referents had no influence on the intention to use collection bins. Neither the overall attitude nor subjective norms of households had influence on their intention to use open pit as a method of solid waste disposal. Overall, attitude of households had stronger influence on their intention to pay for solid waste collection than the subjective norms. Households are willing to pay an average amount of GH ¢4.0 monthly, which is subject to the type of job or income earned by the household heads. Keywords: Solid waste, household, willingness to pay, attitude, subjective norm

Fertilizer Subsidy Programme in Ghana: Evidence of Performance after Six Years of Implementation

This study relied mainly on production data from the Ministry of Food and Agriculture, Ghana and FAOSTATS between 2007 and 2012. Comparative and relative analysis of secondary data on the annual evolution of fertilizer subsidy budgets with total crop production and area expansion trends were used to assess the usefulness or otherwise of the programme. Ghana’s fertilizer subsidy programme still seems to be ineffective almost six years after commencement. Since inception in 2008, a total of GH¢202.5 million has been invested. The evidence however suggests that little has been achieved by way of output growth that can be attributed to fertilizer use. Although the relationship between crop output and budget spending on subsidy was generally positive, it was not significant even at the 10% level, thus weakening the possible impact of the subsidy programme and corroborating claims that most of the recorded growth in agriculture is due to land area expansion as opposed to yield increases. This study alludes to the increasing interest that sustainability concerns must be factored into such subsidy programmes. There is therefore the need for government audit the current programme and fashion out better implementation strategies, possibly adopt the smart approach which has been shown to be more focused and result oriented. Keywords: Subsidy, smart subsidy, fertilizer, food crops, industrial crops, cereals, vegetables, sustainabilit

The relationship of femoral neck shaft angle and adiposity To greater trochanteric pain syndrome in women. A case control morphology and anthropometric study

OBJECTIVE: To evaluate if pelvic or hip width predisposed women to developing greater trochanteric pain syndrome (GTPS). DESIGN: Prospective case control study. PARTICIPANTS: Four groups were included in the study: those gluteal tendon reconstructions (n=31, GTR), those with conservatively managed GTPS (n=29), those with hip osteoarthritis (n=20, OA) and 22 asymptomatic participants (ASC). METHODS: Anterior-posterior pelvic x-rays were evaluated for femoral neck shaft angle; acetabular index, and width at the lateral acetabulum, and the superior and lateral aspects of the greater trochanter. Body mass index, and waist, hip and greater trochanter girth were measured. Data were analysed using a one-way analysis of variance (ANOVA; posthoc Scheffe analysis), then multivariate analysis. RESULTS: The GTR group had a lower femoral neck shaft angle than the other groups (p=0.007). The OR (95% CI) of having a neck shaft angle of less than 134°, relative to the ASC group: GTR=3.33 (1.26 to 8.85); GTPS=1.4 (0.52 to 3.75); OA=0.85 (0.28 to 2.61). The OR of GTR relative to GTPS was 2.4 (1.01 to 5.6). No group difference was found for acetabular or greater trochanter width. Greater trochanter girth produced the only anthropometric group difference (mean (95% CI) in cm) GTR=103.8 (100.3 to 107.3), GTPS=105.9 (100.2 to 111.6), OA=100.3 (97.7 to 103.9), ASC=99.1 (94.7 to 103.5), (ANOVA: p=0.036). Multivariate analysis confirmed adiposity is associated with GTPS. CONCLUSION: A lower neck shaft angle is a risk factor for, and adiposity is associated with, GTPS in women

New genetic signatures associated with cancer cachexia as defined by low skeletal muscle index and weight loss

BACKGROUND: Cachexia affects the majority with advanced cancer. Based on current demographic and clinical factors, it is not possible to predict who will develop cachexia or not. Such variation may, in part, be due to genotype. It has recently been proposed to extend the diagnostic criteria for cachexia to include a direct measure of low skeletal muscle index (LSMI) in addition to weight loss (WL). We aimed to explore our panel of candidate single nucleotide polymorphism (SNPs) for association with WL +/− computerized tomography‐defined LSMI. We also explored whether the transcription in muscle of identified genes was altered according to such cachexia phenotype METHODS: A retrospective cohort study design was used. Analysis explored associations of candidate SNPs with WL (n = 1276) and WL + LSMI (n = 943). Human muscle transcriptome (n = 134) was analysed using an Agilent platform. RESULTS: Single nucleotide polymorphisms in the following genes showed association with WL alone: GCKR, LEPR, SELP, ACVR2B, TLR4, FOXO3, IGF1, CPN1, APOE, FOXO1, and GHRL. SNPs in LEPR, ACVR2B, TNF, and ACE were associated with concurrent WL + LSMI. There was concordance between muscle‐specific expression for ACVR2B, FOXO1 and 3, LEPR, GCKR, and TLR4 genes and LSMI and/or WL (P < 0.05). CONCLUSIONS: The rs1799964 in the TNF gene and rs4291 in the ACE gene are new associations when the definition of cachexia is based on a combination of WL and LSMI. These findings focus attention on pro‐inflammatory cytokines and the renin–angiotensin system as biomarkers/mediators of muscle wasting in cachexia

Embedded rehabilitation in major trauma: Retrospective pre-post observational study of service and patient outcomes

Introduction: Major trauma describes serious and often multiple injuries where there is a strong possibility of death or residual disability. There is little robust evidence on the effects of embedded rehabilitation within the trauma care pathway. Trauma rehabilitation services therefore remain fragmented and poorly integrated. This study aimed to determine changes in hospital length of stay (LoS), intensive care unit (ICU) LoS, 30-day mortality and Glasgow Outcome Scale following implementation of an embedded rehabilitation service into a Major Trauma Centre (MTC). Methods: Retrospective pre-post observational study of a rehabilitation service introduced into an MTC, consisting of a dedicated 10-bedded inpatient unit, co-ordinating rehabilitation hub, and specialist multi-disciplinary outpatient clinic. Overall hospital LoS, ICU LoS, 30-day mortality and GOS were selected as outcome measures. Patient characteristics (age, sex, injury mechanism, injury severity score, Glasgow Coma Scale, and most injured body region) were compared and controlled for when analysing outcomes. Results: The study cohort included 6,484 patients, of which 4,298 were pre-intervention and 2,186 post-intervention. Patients in the post-intervention cohort were older than those in the pre-intervention cohort (58.3 compared to 56.6, p15 compared to 43.9% >15). Moderate but significant changes to the most injured body region were also observed (p<0.001), with fewer injuries affecting the limbs (25.8% to 24.9%), spine (15.3% to 12.1%), multiple locations (11.3% to 10.7%), abdomen (2.7% to 2.4%) and face/other (1.9% to 1.5%) and more injuries affecting the head (27.5% to 31.5%) and chest (15.6% to 16.9%). Controlling for changes to patient characteristics between the two time periods, there was a reduction in overall hospital LoS of 2.56 days (b=-2.56, p<0.001) and ICU LoS of 0.94 days (b=-0.96, p<0.001). There was a 31% reduced chance of 30-day mortality in post-intervention patients (OR=0.69, 95%CI=0.54 to 0.88), and almost two times higher relative chance of GOS Good Recovery (RR=1.94, CI=1.51 to 2.49). Discussion: Embedded rehabilitation is an important and necessary component of an effective trauma system that is associated with improved service and patient outcomes. Future research should examine prospectively how a dedicated rehabilitation service affects medium- and long-term patient-centred outcomes

Discovery and development strategies for SARS-CoV-2 NSP3 macrodomain inhibitors

The worldwide public health and socioeconomic consequences caused by the COVID-19 pandemic highlight the importance of increasing preparedness for viral disease outbreaks by providing rapid disease prevention and treatment strategies. The NSP3 macrodomain of coronaviruses including SARS-CoV-2 is among the viral protein repertoire that was identified as a potential target for the development of antiviral agents, due to its critical role in viral replication and consequent pathogenicity in the host. By combining virtual and biophysical screening efforts, we discovered several experimental small molecules and FDA-approved drugs as inhibitors of the NSP3 macrodomain. Analogue characterisation of the hit matter and crystallographic studies confirming binding modes, including that of the antibiotic compound aztreonam, to the active site of the macrodomain provide valuable structure–activity relationship information that support current approaches and open up new avenues for NSP3 macrodomain inhibitor development

Intracoronary and retrograde coronary venous myocardial delivery of adipose-derived stem cells in swine infarction lead to transient myocardial trapping with predominant pulmonary redistribution

OBJECTIVES: To examine the comparative fate of adipose-derived stem cells (ASCs) as well as their impact on coronary microcirculation following either retrograde coronary venous (RCV) or arterial delivery. BACKGROUND: Local delivery of ASCs to the heart has been proposed as a practical approach to limiting the extent of myocardial infarction. Mouse models of mesenchymal stem cell effects on the heart have also demonstrated significant benefits from systemic (intravenous) delivery, prompting a question about the advantage of local delivery. There has been no study addressing the extent of myocardial vs. systemic disposition of ASCs in large animal models following local delivery to the myocardium. METHODS: In an initial experiment, dose-dependent effects of ASC delivery on coronary circulation in normal swine were evaluated to establish a tolerable ASC dosing range for intracoronary (IC) delivery. In a set of subsequent experiments, an anterior acute myocardial infarction (AMI) was created by balloon occlusion of the proximal left anterior descending (LAD) artery, followed by either IC or RCV infusion of 10(7) (111)Indium-labeled autologous ASCs 6 days following AMI. Indices of microcirculatory resistance (IMR) and coronary flow reserve (CFR) were measured before sacrifices to collect tissues for analysis at 1 or 24 hr after cell delivery. RESULTS: IC delivery of porcine ASCs to normal myocardium was well tolerated up to a cumulative dose of 14 × 10(6) cells (approximately 0.5 × 10(6) cells/kg). There was evidence suggesting microcirculatory trapping of ASC: at unit doses of 50 × 10(6) ASCs, IMR and CFR were found to be persistently altered in the target LAD distribution at 7 days following delivery, whereas at 10 × 10(6) ASCs, only CFR was altered. In the context of recent MI, a significantly higher percentage of ASCs was retained at 1 hr with IC delivery compared with RCV delivery (57.2 ± 12.7% vs. 17.9 ± 1.6%, P = 0.037) but this initial difference was not apparent at 24 hr (22.6 ± 5.5% vs. 18.7 ± 8.6%; P = 0.722). In both approaches, most ASC redistributed to the pulmonary circulation by 24 hr postdelivery. There were no significant differences in CFR or IMR following ASC delivery to infarcted tissue by either route. CONCLUSIONS: Selective intravascular delivery of ASC by coronary arterial and venous routes leads to similarly limited myocardial cell retention with predominant redistribution of cells to the lungs. IC arterial delivery of ASC leads to only transiently greater myocardial retention, which is accompanied by obstruction of normal regions of coronary microcirculation at higher doses. The predominant intrapulmonary localization of cells following local delivery via both methods prompts the notion that systemic delivery of ASC might provide similarly beneficial outcomes while avoiding risks of inadvertent microcirculatory compromise

Strengthening Routine Data Systems to Track the HIV Epidemic and Guide the Response in Sub-Saharan Africa

The global HIV response has entered a new phase with the recommendation of treating all persons living with HIV with antiretroviral therapy, and with the goals of reducing new infections and AIDS-related deaths to fewer than 500,000 by 2020. This new phase has intensive data requirements that will need to utilize routine data collected through service delivery platforms to monitor progress toward these goals. With a focus on sub-Saharan African, we present the following priorities to improve the demand, supply, and use of routine HIV data: (1) strengthening patient-level HIV data systems that support continuity of clinical care and document sentinel events; (2) leveraging data from HIV testing programs; (3) using targeting data collection in communities and among clients; and (4) building capacity and promoting a culture of HIV data quality assessment and use. When fully leveraged, routine data can efficiently provide timely information at a local level to inform action, as well as provide information at scale with wide geographic coverage to strengthen estimation efforts

Adapting and testing a brief intervention to reduce maternal anxiety during pregnancy (ACORN): study protocol for a randomised controlled trial.

BACKGROUND: National guidelines in the UK, United States of America, Canada, and Australia have recently stressed the importance of identifying and treating antenatal anxiety and depression. However, there is little research into the most effective and acceptable ways of helping women manage their symptoms of anxiety and stress during pregnancy. Research indicates the necessity to consider the unique needs and concerns of perinatal populations to ensure treatment engagement, highlighting the need to develop specialised treatments which could be integrated within routine antenatal healthcare services. This trial aims to develop a brief intervention for antenatal anxiety, with a focus on embedding the delivery of the treatment within routine antenatal care. METHODS/DESIGN: This study is a two-phase feasibility trial. In phase 1 we will develop and pilot a brief intervention for antenatal anxiety, blended with group support, to be led by midwives. This intervention will draw on cognitive behavioural principles and wider learning from existing interventions that have been used to reduce anxiety in expectant mothers. The intervention will then be tested in a pilot randomised controlled trial in phase 2. The following outcomes will be assessed: (1) number of participants meeting eligibility criteria, (2) number of participants consenting to the study, (3) number of participants randomised, (4) number of sessions completed by those in the intervention arm, and (5) number of participants completing the post-intervention outcome measures. Secondary outcomes comprise: detailed feedback on acceptability, which will guide further development of the intervention; and outcome data on symptoms of maternal and paternal anxiety and depression, maternal quality of life, quality of couple relationship, mother-child bonding, infant temperament and infant sleep. DISCUSSION: The study will provide important data to inform the design of a future full-scale randomised controlled trial of a brief intervention for anxiety during pregnancy. This will include information on its acceptability and feasibility regarding implementation within current antenatal services, which will inform whether ultimately this provision could be rolled out widely in healthcare settings. TRIAL REGISTRATION: Current Controlled Trials ISRCTN95282830 . Registered on 29 October 2014