Glampingholic: plan de viabilidad de un complejo tipo glamping en Riaño, León = Glampingholic: viability study of a glamping site in Riaño, León (spain)

El turismo es considerado uno de los sectores más importantes a nivel mundial y, hoy en día, muchos países tienen una economía basada, principalmente, en el sector turístico. Debido a su crecimiento continuo y al crecimiento exponencial del número de turistas, la industria del turismo busca nuevos nichos de mercado cuya oferta satisfaga las necesidades de perfiles de turistas cada vez más exigentes. Por este motivo, este Trabajo de Fin de Grado (TFG) analizará una nueva tendencia, la cual aúna la diferenciación y especialización de sus servicios en el mercado del sector turístico: el Glamping. En este proyecto se propone la creación de un nuevo complejo mediante la tipología glamping en un paraje situado cerca del Parque Nacional Picos de Europa. Para ello, en primer lugar, se llevará a cabo la creación de la empresa. En segundo lugar, se llevará a cabo la compra de los Glass Igloo como tipo específico de alojamiento incluyendo todo el material necesario para el desarrollo de la actividad y la contratación de personal. Y por último, se evalúa la inversión con una proyección de 5 años para, finalmente, poder determinar si se trata de un proyecto viable o no

H2O2-preconditioned human adipose-derived stem cells (HC016) increase theirresistance to oxidative stress byoverexpressing Nrf2 and bioenergetic adaptation

BackgroundMesenchymal stem cells, including those derived from human adipose tissue (hASCs), are currently being widely investigated for cell therapy. However, when transplanted at the site of injury, the survival and engraftment rates of hASCs are low, mainly due to the harsh microenvironment they encounter, characterized by inflammation and oxidative stress. To overcome these therapeutic limitations, cell preconditioning with low-concentration of hydrogen peroxide (H2O2) has been proposed as a plausible strategy to increase their survival and adaptation to oxidative stress. Nonetheless, the underlying mechanisms of this approach are not yet fully understood. In this study, we analyzed molecular and bioenergetic changes that take place in H2O2 preconditioned hASCs.MethodsLong-term exposure to a low concentration of H2O2 was applied to obtain preconditioned hASCs (named HC016), and then, their response to oxidative stress was analyzed. The effect of preconditioning on the expression of Nrf2 and its downstream antioxidant enzymes (HO-1, SOD-1, GPx-1, and CAT), and of NF-kappa B and its related inflammatory proteins (COX-2 and IL-1 beta), were examined by Western blot. Finally, the Seahorse XF96 Flux analysis system was used to evaluate the mitochondrial respiration and glycolytic function, along with the total ATP production.ResultsWe found that under oxidative conditions, HC016 cells increased the survival by (i) decreasing intracellular ROS levels through the overexpression of the transcription factor Nrf2 and its related antioxidant enzymes HO-1, SOD-1, GPx-1, and CAT; (ii) reducing the secretion of pro-inflammatory molecules COX-2 and IL-1 beta through the attenuation of the expression of NF-kappa B; and (iii) increasing the total ATP production rate through the adaption of their metabolism to meet the energetic demand required to survive.ConclusionsH(2)O(2) preconditioning enhances hASC survival under oxidative stress conditions by stimulating their antioxidant response and bioenergetic adaptation. Therefore, this preconditioning strategy might be considered an excellent tool for strengthening the resistance of hASCs to harmful oxidative stress.Partial funding for this project was provided by the Department of Economic Development and Competitiveness of the Basque Government, the European Regional Development Fund (PREMISE IG-2015/0000558), and the University of the Basque Country (UPV/EHU; research grants PES 17/29 and 16/37)

Construction of a plasmid vector based on the pMV158 replicon for cloning and inducible gene expression in Streptococcus pneumoniae

18 p.-4 fig.We report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae. The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter PM of the S. pneumoniaemalMP operon, followed by a multi-cloning site sequence aimed to facilitate the insertion of target genes. The expression from PM is negatively regulated by the transcriptional repressor MalR, which is released from the DNA operator sequence by growing the cells in maltose-containing media. To get a highly regulated expression of the target gene, MalR was provided in cis by inserting the malR gene under control of the constitutive Ptet promoter, which in pMV158 directs expression of the tetL gene. To test the functionality of the system, we cloned the reporter gene gfp from Aequorea victoria, encoding the green fluorescent protein (GFP). Pneumococcal cells harboring the recombinant plasmid rendered GFP fluorescence in a maltose-dependent mode with undetectable background levels in the absence of the inducer. The new vector, pLS1ROM, exhibits full structural and segregational stability and constitutes a valuable tool for genetic manipulation and regulated gene expression in S. pneumoniaeThis study was supported by the Spanish Ministerio de Ciencia e Innovación (Grants CSD2008-00013, INTERMODS to ME; BFU2007-63575 and BFU2010-19597, PNEUMOTALK to GdS) and the European Union (Grant EU-CP223111, CAREPNEUMO to ME)Peer reviewe

Hydrogen Peroxide-Preconditioned Human Adipose-Derived Stem Cells Enhance the Recovery of Oligodendrocyte-Like Cells after Oxidative Stress-Induced Damage

Oxidative stress associated with neuroinflammation is a key process involved in the pathophysiology of neurodegenerative diseases, and therefore, has been proposed as a crucial target for new therapies. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for neuroprotection. These cells can be preconditioned by exposing them to mild stress in order to improve their response to oxidative stress. In this study, we evaluate the therapeutic potential of hASCs preconditioned with low doses of H2O2 (called HC016 cells) to overcome the deleterious effect of oxidative stress in an in vitro model of oligodendrocyte-like cells (HOGd), through two strategies: i, the culture of oxidized HOGd with HC016 cell-conditioned medium (CM), and ii, the indirect co-culture of oxidized HOGd with HC016 cells, which had or had not been exposed to oxidative stress. The results demonstrated that both strategies had reparative effects, oxidized HC016 cell co-culture being the one associated with the greatest recovery of the damaged HOGd, increasing their viability, reducing their intracellular reactive oxygen species levels and promoting their antioxidant capacity. Taken together, these findings support the view that HC016 cells, given their reparative capacity, might be considered an important breakthrough in cell-based therapies.Partial funding for this project was provided by the Department of Economic Development and Competitiveness of the Basque Government, the European Regional Development Fund (PREMISE IG-2015/0000558) and the University of the Basque Country (UPV/EHU; research grants GIU 19/088 and PES 17/29 and 16/37)

Conditioned Medium from H2O2-Preconditioned Human Adipose-Derived Stem Cells Ameliorates UVB-Induced Damage to Human Dermal Fibroblasts

Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidative modifications of cellular components and biomolecules involved in the metabolism of dermal cells. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for photoprotection due to their pro-angiogenic properties, protective activity against oxidative stress and paracrine effect on dermal cells. To enhance these therapeutic properties, hASCs can be preconditioned by exposing them to sublethal cellular stressors. In this study, we first analyzed response capacity against UVB-induced oxidative stress in H2O2-preconditioned hASCs (called HC016 cells); and second, we evaluated the photoprotective effect of HC016-conditioned medium (CM) in an in vitro UVB irradiation model in cultured human foreskin fibroblasts (hFFs). The results demonstrated that HC016 cells have a greater capacity to respond efficiently to UVB-induced oxidative stress, evidenced by higher Nrf2 antioxidant system activity and enhanced viability and migration capacity. Further, HC016-CM treatment increased viability, migratory capacity and collagen type I synthesis in hFFs exposed to UVB radiation, as well as reducing their cytotoxicity, apoptosis, senescence and IL-6 secretion. Collectively, these findings support the view that HC016 cells could protect against UVB-induced photodamage via paracrine mechanisms.This research was founded by the University of the Basque Country (UPV/EHU, grant reference numbers: GIU19/088 and PES 21/50)

Factors associated with hospitalization after suicide spectrum behaviors: results from a multicentre study in Spain

Objectives: to identify factors associated with admission after suicide spectrum behaviors. Methods: Patient's characteristics, the nature of their suicidal behavior, admission rates between centres, and factors associated with admission have been examined in suicide spectrum presentations to emergency departments in three Spanish cities. Results: Intent of the suicidal behavior had the greatest impact on hospitalization. Older age, living alone, self-harm method not involving drug overdose, previous history of suicide spectrum behaviors and psychiatric diagnosis of schizophrenia, mood or personality disorder were independently associated with being admitted. There was a three-fold between-centre difference in the rate of hospitalization. Conclusions: widespread differences in the rate of hospitalization were primarily accounted for by characteristics of the individual patients and their suicidal behavior

Consumo de alcohol y factores de riesgo de conductas autolesivas en adolescentes españoles

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Construyendo un lenguaje común entre docentes de educación inicial y terapeutas que participan de integraciones de niños con autismo

In the integration experiences the author has gone through, he has noticed a great difficulty to be able to understand that, due to the special educational needs that children suffering of autism possess (Widespread Overturn of the Development), it is indispensable the use of specific strategies to enlarge the development at cognitive and social level of these children. Therefore some resistances usually appear, on the part of teachers and directive of initial education, in front of the use of certain strategies because they cannot discover the similarities there are among some of the resources habitually used by them in their classes and the proposals of the integrative teachers (that in our institution use the strategies of cognitive-behavioral approach). The essential difference among these proposals lies in the necessity that children with autism have of a systematizing and constant organization of the supports to avoid that they could be disorganized or isolated.En las experiencias de integración de las que he participado he notado una gran dificultad para poder comprender que, debido a las necesidades educativas especiales que poseen los niños con Autismo-TGD (Trastorno Generalizado del Desarrollo), resulta imprescindible el uso de estrategias específicas para ampliar el desarrollo a nivel cognitivo y social de estos niños en el nivel inicial.  Por lo tanto suelen aparecer algunas resistencias, por parte de docentes y directivos de educación inicial, ante el uso de determinadas estrategias debido a que no pueden descubrir las similitudes que existen entre algunos de los recursos utilizados habitualmente por ellos en sus clases y las propuestas de los maestros integradores (que en nuestro Centro se valen de estrategias del abordaje cognitivo-conductual). La diferencia esencial entre estas propuestas se encuentra en la necesidad que tienen los niños con Autismo-TGD de una sistematización y organización constante de los apoyos para evitar que puedan desorganizarse o aislarse

Construction of a plasmid vector based on the pMV158 replicon for cloning and inducible gene expression in Streptococcus pneumoniae

18 p.-4 fig.We report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae. The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter PM of the S. pneumoniaemalMP operon, followed by a multi-cloning site sequence aimed to facilitate the insertion of target genes. The expression from PM is negatively regulated by the transcriptional repressor MalR, which is released from the DNA operator sequence by growing the cells in maltose-containing media. To get a highly regulated expression of the target gene, MalR was provided in cis by inserting the malR gene under control of the constitutive Ptet promoter, which in pMV158 directs expression of the tetL gene. To test the functionality of the system, we cloned the reporter gene gfp from Aequorea victoria, encoding the green fluorescent protein (GFP). Pneumococcal cells harboring the recombinant plasmid rendered GFP fluorescence in a maltose-dependent mode with undetectable background levels in the absence of the inducer. The new vector, pLS1ROM, exhibits full structural and segregational stability and constitutes a valuable tool for genetic manipulation and regulated gene expression in S. pneumoniaeThis study was supported by the Spanish Ministerio de Ciencia e Innovación (Grants CSD2008-00013, INTERMODS to ME; BFU2007-63575 and BFU2010-19597, PNEUMOTALK to GdS) and the European Union (Grant EU-CP223111, CAREPNEUMO to ME)Peer reviewe

Celeberrimo Sisaponensi regione in Baetica miniario metallo… Vías de investigación sobre el cinabro hispano en época romana

El cinabrio (HgS) en época romana fue un producto muy apreciado por su uso como pigmento rojo en la pintura mural, convirtiéndose, gracias a su elevado precio, en un indicador de estatus. Pero sus aplicaciones no acaban aquí, dada la capacidad del mercurio (Hg) para amalgamar metales nobles, lo que explicaría el férreo control de la explotación y gestión del mineral por el Estado romano. Su papel en la economía antigua se ha analizado tradicionalmente desde la interpretación de las fuentes escritas, pero la aplicación de técnicas arqueométricas sobre diferentes objetos de estudio abre nuevas vías para dimensionar su producción y difusión. En esta tarea colabora también el estudio diacrónico de la ciudad que gestionó su beneficio -Sisapo- y la investigación sobre su territorio de explotación. En este trabajo se sintetiza el avance en el conocimiento sobre estos tópicos de investigación realizados en el marco del proyecto Sisapo, iniciado por Carmen Fernández Ochoa y en el que ha trabajado desde sus inicios.In Roman times, cinnabar (HgS) was a highly valued product because of its use as a red pigment in wall painting, thus becoming a status indicator due to its high price. But its applications do not end here, since mercury (Hg) could also be used in the amalgamation of noble metals. This fact would explain the tight control of the exploitation and manage-ment of this mineral by the Roman State. The role of cinnabar in the Roman economy has been traditionally analysed through the interpretation of written sources, but the application of archaeometric techniques on different objects of study opens new ways to measure its extraction and diffusion. The diachronic study of the town which managed its profit - Sisapo - and the research of its territory of exploitation also contributes to this task. In this paper we synthesize the advancement of knowledge about these research topics developed in the framework of the Sisapo project, initiated by Carmen Fernández Ochoa and on which she has worked since its beginning