76 research outputs found
Molecular dynamics study of contact mechanics: contact area and interfacial separation from small to full contact
We report a molecular dynamics study of the contact between a rigid solid
with a randomly rough surface and an elastic block with a flat surface. We
study the contact area and the interfacial separation from small contact (low
load) to full contact (high load). For small load the contact area varies
linearly with the load and the interfacial separation depends logarithmically
on the load. For high load the contact area approaches to the nominal contact
area (i.e., complete contact), and the interfacial separation approaches to
zero. The present results may be very important for soft solids, e.g., rubber,
or for very smooth surfaces, where complete contact can be reached at moderate
high loads without plastic deformation of the solids.Comment: 4 pages,5 figure
Fluid flow at the interface between elastic solids with randomly rough surfaces
I study fluid flow at the interface between elastic solids with randomly
rough surfaces. I use the contact mechanics model of Persson to take into
account the elastic interaction between the solid walls and the Bruggeman
effective medium theory to account for the influence of the disorder on the
fluid flow. I calculate the flow tensor which determines the pressure flow
factor and, e.g., the leak-rate of static seals. I show how the perturbation
treatment of Tripp can be extended to arbitrary order in the ratio between the
root-mean-square roughness amplitude and the average interfacial surface
separation. I introduce a matrix D(Zeta), determined by the surface roughness
power spectrum, which can be used to describe the anisotropy of the surface at
any magnification Zeta. I present results for the asymmetry factor Gamma(Zeta)
(generalized Peklenik number) for grinded steel and sandblasted PMMA surfaces.Comment: 16 pages, 14 figure
Rubber friction on (apparently) smooth lubricated surfaces
We study rubber sliding friction on hard lubricated surfaces. We show that
even if the hard surface appears smooth to the naked eye, it may exhibit short
wavelength roughness, which may give the dominant contribution to rubber
friction. That is, the observed sliding friction is mainly due to the
viscoelastic deformations of the rubber by the substrate surface asperities.
The presented results are of great importance for rubber sealing and other
rubber applications involving (apparently) smooth surfaces.Comment: 7 pages, 15 figure
Contact mechanics: contact area and interfacial separation from small contact to full contact
We present a molecular dynamics study of the contact between a rigid solid
with a randomly rough surface and an elastic block with a flat surface. The
numerical calculations mainly focus on the contact area and the interfacial
separation from small contact (low load) to full contact (high load). For small
load the contact area varies linearly with the load and the interfacial
separation depends logarithmically on the load. For high load the contact area
approaches the nominal contact area (i.e., complete contact), and the
interfacial separation approaches zero. The numerical results have been
compared with analytical theory and experimental results. They are in good
agreement with each other. The present findings may be very important for soft
solids, e.g., rubber, or for very smooth surfaces, where complete contact can
be reached at moderate high loads without plastic deformation of the solids.Comment: 15 pages, 23 figure
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults
Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults
Genetic mapping of high caries experience on human chromosome 13
Background: Our previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries.Methods: Seventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals.Results: Statistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence.Conclusions: Genetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored. © 2013 Küchler et al.; licensee BioMed Central Ltd
Heterogeneous Multiscale Methods for modelling surface topography in Elastohydrodynamic Lubrication line contacts
A multiscale method for the Elastohydrodynamic Lubrication (EHL) of line contacts is derived based on the Heterogeneous Multiscale Methods. Periodicity applies to the topographical features and lubricant flow, data is homogenised over a range of variables at a micro-scale and coupled into a macro-scale model. This is achieved using flow factors as calculated from metamodels, which themselves evolve with the solution procedure. Results are given for an idealised topography and illustrate significant deviations from smooth surface assumptions as quantified by the flow factors. Improvements in the accuracy and efficiency with previous work and large fluctuations due to micro-EHL are also presented. Validation of the multiscale method with a deterministic topography is provided demonstrating good accuracy and efficiency
Significance of genetic variations in developmental enamel defects of primary dentition in Polish children
Systemic pro-inflammatory response facilitates the development of cerebral edema during short hypoxia
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