The physiology of ex vitro pineapple ( Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by environmental conditions: proteomic and transcriptomic profiles

Pineapple is one of the most important tropical crops worldwide. The use of Temporary Immersion Bioreactors (TIB) for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are carefully acclimatized to adapt to natural environmental conditions, and a facultative C3/CAM metabolism in the first two months of growth is characteristic of pineapple plants, depending on environmental conditions. We subjected two sets of micropropagated pineapple plants to C3 and CAM-inducing environmental conditions, determined by light intensity/relative humidity (respectively 40μmol.m-2s-1/85% and 260μmol.m-2.s-1/50%). Leaves of pineapple plants grown under CAM inducing conditions showed higher leaf thickness and more developed cuticles and hypodermic tissue. Proteomic profiles of several proteins, isoenzyme patterns and transcriptomic profiles were also measured. Five major spots were isolated and identified, two of them for the first time in Ananas comosus (OEE 1; OEE 2) and the other three corresponding to small fragments of the large subunit of Rubisco (LSU). PEPC and PEPCK were also detected by immunobloting of 2DE at the end of both ex vitro treatments (C3/CAM) during the dark period. Isoenzymes of SOD and CAT were identified by electrophoresis and the transcript levels of OEE 1 and CAT were associated with CAM metabolism in pineapple plants

Effects of hydrogen and primary air in a commercial partially-premixed atmospheric gas burner by means of optical and supervised machine learning techniques

In order to ascertain the effects of the hydrogen addition and the primary air-fuel ratio on burner performance and emissions, we conduct tests on a commercial atmospheric gas burner using pure methane and a blend of hydrogen/methane. Relevant statistical image features are extracted from a UV–VIS camera equipped with narrow-band optical filters. Radical image results agrees with spectrometric data, showing the relevance of the OH* intensity radiation coming from the outer non-premixed zone. The double-cone flame structure is evident, showing a growing secondary non-premixed cone as the primary air-fuel ratio is decreased. In addition, the direct relationship found between flame radical imaging features and NOx emissions has been used to develop a predictive model by integrating classification techniques and neural networks. The research confirms UV–VIS chemiluminescence imaging techniques as powerful tools aimed at combustion monitoring, with huge prospects of being integrated within advanced emission control techniques for commercial burners

Assessing the risk of an emerging zoonosis of worldwide concern : anisakiasis

The authors sincerely thank the Biobanking platform at the PARASITE project (EU FP7 PARASITE project (GA no. 312068)) for providing host-parasite data. We thank Rosa Fernández and Cristina Martínez from CETMAR for their help during creation and divulgation of the questionnaires. We also thank Arturo del Rey Moreno (“Antequera” hospital) for his helpful comments. We are also grateful to “Subdirección General de Economía Pesquera” of “Ministerio de Agricultura, Alimentación y Medio Ambiente” (MAGRAMA) of the Spanish government for providing anchovy trade statistics for 2013. M. Bao is supported by a PhD grant from the University of Aberdeen and also by financial support of the contract from the EU Project PARASITE (grant number 312068).Peer reviewedPublisher PD

Catalytic Asymmetric Synthesis of Bicycloprolines by a 1,3-Dipolar Cycloaddition/Intramolecular Alkylation Strategy

This document is the Accepted Manuscript version of a Published Work that appeared in final form inJournal of Organic Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.joc.6b01100 to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.htmlThe diastereoselective one-pot synthesis of hexahydrocyclopenta [b] pyrrole derivatives (bicycloprolines) has been achieved by base-mediated reactions of (E)-tert-butyl 6-bromo-2-hexenoate with α-imino esters. The catalytic asymmetric version of this process has been efficiently achieved using the CuI/(R)-DTBM-Segphos complex as a catalyst following a two-step 1,3-dipolar cycloaddition/intramolecular alkylation sequence.Financial support of this work by the Ministerio de Economía y Competitividad and Fondo Europeo de Desarrollo Regional (MINECO CTQ2012-35790 and MINECO/FEDER CTQ2015-66954-P) are gratefully acknowledged. M.G.-E. and A.P.-E. thank the MICINN for predoctoral fellowship

Un SIG corporativo en el IGN para la gestión integrada, publicación y análisis de datos geográficos

El Instituto Geográfico Nacional inició en el año 2003 los estudios previos necesarios para un proyecto ambicioso y a la vez esencial para el cumplimiento de sus responsabilidades básicas, la implantación de un Sistema de Información Geográfica (SIG) corporativo que atendiera a tres finalidades fundamentales: a) la gestión integrada de los datos geográficos y cartográficos que produce, en forma de Bases Cartográficas Numéricas (BCN) y en forma de mapas, respectivamente, incluyendo su actualización coordinada; b) la publicación de tales datos en papel, en soporte digital y de manera electrónica (Infraestructura de Datos Espaciales (IDE)); c) el análisis y consulta desde clientes ligeros y pesados para todo tipo de aplicaciones. Por lo tanto, el sistema constituye a la vez el soporte básico para la publicación de cartografía y para la consulta de los datos geográficos desde clientes a través de la Web,mediante el desarrollo de servicios de análisis

AB0689 The importance of the sun. Vitamin D and spondyloarthritis: our experience in a third level hospital

[EN] Vitamin D plays an important role in the pathogenesis of autoimmune diseases, so that it has been shown that an adequate level is associated with a lower risk of developing this group of entities as well as a lower severity of them. Specifically, in spondyloarthritis (SpA) the deficiency has been associated with greater aggressiveness and greater radiological progression.S

AB0690 Antiphospholipid antibodies and spondyloarthritis. Truth or myth? Our results in a third level hospital

[EN] The importance of antiphospholipid antibodies and their clinical involvement in thrombotic phenomena, isolated or associated with certain autoimmune diseases such as systemic lupus erythematosus, is known. However, in spondyloarthritis (SpA) there is little published data about it.S

Processing and mechanical properties of novel biodegradable poly-lactic acid/Zn 3D printed scaffolds for application in tissue regeneration

The feasibility to manufacture scaffolds of poly-lactic acid reinforced with Zn particles by fused filament fabrication is demonstrated for the first time. Filaments of 2.85 mm in diameter of PLA reinforced with different weight fractions of $\mu$m-sized Zn - 1 wt. \% Mg alloy particles (in the range 3.5 to 17.5 wt. \%) were manufactured by a double extrusion in method in which standard extrusion is followed by a precision extrusion in a filament-maker machine. Filaments with constant diameter, negligible porosity and a homogeneous reinforcement distribution were obtained for Zn weight fractions of up to 10.5\%. It was found that the presence of Zn particles led to limited changes in the physico-chemical properties of the PLA that did not affect the window temperature for 3D printing nor the melt flow index. Thus, porous scaffolds could be manufactured by fused filament fabrication at 190\textdegree C with poly-lactic acid/Zn composites containing 3.5 and 7 wt. \% of Zn and at 170\textdegree C when the Zn content was 10.5 wt. \% with excellent dimensional accuracy and mechanical properties