The effect of green tea extract on the sperm parameters and histological changes of testis in rats exposed to para-nonylphenol

Background: Para-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals. Objective: The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats. Materials and Methods: 24 adult male Wistar rats (215 ± 20 gr) were randomly divided into four groups (n = 6/each) – including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE – and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated. Results: A significant decrease in the sperm number, motility, viability and morphology (p < 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p < 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p < 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE. Conclusion: It seems GTE can be used as a potent antioxidant in the case of p-NP toxication. Key words: Green tea extract, Para-nonylphenol, Sperm, Testis, Rat

Phenotypic and molecular effects of chronic gamma irradiation on Curcuma alismatifolia

Mutation breeding is one of the methods for generating genetic variation and obtaining new cultivars of ornamental plants during the past decades. In present study, the effects of four doses (0, 14.6, 33, and 87.4 Gy) of chronic gamma irradiation on three cultivars of Curcuma alismatifolia and one Curcuma hybrid were investigated. Morphological aberrations from non-treated plants were observed by exposing growing plants. Higher doses induced phenotypical variations and significantly affected the plant growth parameters and flowering capacity. In terms of genetic variation, among the irradiated cultivars, the number of presumed alleles revealed by SSR analysis ranged from two to five with a mean value of 3.1 to 3.7 alleles per locus for radiation doses. The average value of the effective number of alleles, Nei’s gene diversity, and Shannon’s information index were 2.42–2.66, 0.50–0.56, and 0.90–1.03, respectively. Heat map hierarchical clustering divided 52 studied individuals into four major clusters. Results of this study showed that chronic gamma irradiation efficiently can enhance the phenotypical and genetic variations in C. alismatifolia cultivars at doses of 33 Gy and 84.6 Gy. In addition, SSR markers will likely accelerate the progress of selection of desired mutants during mutation breeding programs

TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding

Induced mutations have been used effectively for plant improvement. Physical and chemical mutagens induce a high frequency of genome variation. Recently, developed screening methods have allowed the detection of single nucleotide polymorphisms (SNPs) and the identification of traits that are difficult to identify at the molecular level by conventional breeding. With the assistance of reverse genetic techniques, sequence variation information can be linked to traits to investigate gene function. Targeting induced local lesions in genomes (TILLING) is a high-throughput technique to identify single nucleotide mutations in a specific region of a gene of interest with a powerful detection method resulted from chemical-induced mutagenesis. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of genome size and ploidy level. However, TILLING requires laborious and time-consuming steps, and a lack of complete genome sequence information for many crop species has slowed the development of suitable TILLING targets. Another method, high-resolution melting (HRM), which has assisted TILLING in mutation detection, is faster, simpler and less expensive with non-enzymatic screening system. Currently, the sequencing of crop genomes has completely changed our vision and interpretation of genome organization and evolution. Impressive progress in next-generation sequencing (NGS) technologies has paved the way for the detection and exploitation of genetic variation in a given DNA or RNA molecule. This review discusses the applications of TILLING in combination with HRM and NGS technologies for screening of induced mutations and discovering SNPs in mutation breeding programs.Peer reviewe

Validity and reliability of the Iranian version of the Pediatric Quality of Life Inventory™ 4.0 (PedsQL™) Generic Core Scales in children

<p>Abstract</p> <p>Background</p> <p>This study aimed to investigate the reliability and validity of the Iranian version of the Pediatric Quality of Life Inventory™ 4.0 (PedsQL™ 4.0) Generic Core Scales in children.</p> <p>Methods</p> <p>A standard forward and backward translation procedure was used to translate the US English version of the PedsQL™ 4.0 Generic Core Scales for children into the Iranian language (Persian). The Iranian version of the PedsQL™ 4.0 Generic Core Scales was completed by 503 healthy and 22 chronically ill children aged 8-12 years and their parents. The reliability was evaluated using internal consistency. Known-groups discriminant comparisons were made, and exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were conducted.</p> <p>Results</p> <p>The internal consistency, as measured by Cronbach's alpha coefficients, exceeded the minimum reliability standard of 0.70. All monotrait-multimethod correlations were higher than multitrait-multimethod correlations. The intraclass correlation coefficients (ICC) between the children self-report and parent proxy-reports showed moderate to high agreement. Exploratory factor analysis extracted six factors from the PedsQL™ 4.0 for both self and proxy reports, accounting for 47.9% and 54.8% of total variance, respectively. The results of the confirmatory factor analysis for 6-factor models for both self-report and proxy-report indicated acceptable fit for the proposed models. Regarding health status, as hypothesized from previous studies, healthy children reported significantly higher health-related quality of life than those with chronic illnesses.</p> <p>Conclusions</p> <p>The findings support the initial reliability and validity of the Iranian version of the PedsQL™ 4.0 as a generic instrument to measure health-related quality of life of children in Iran.</p

Allele mining strategies: principles and utilisation for blast resistance genes in rice (Oryza sativa L.)

Allele mining is a promising way to dissect naturally occurring allelic variants of candidate genes with essential agronomic qualities. With the identification, isolation and characterisation of blast resistance genes in rice, it is now possible to dissect the actual allelic variants of these genes within an array of rice cultivars via allele mining. Multiple alleles from the complex locus serve as a reservoir of variation to generate functional genes. The routine sequence exchange is one of the main mechanisms of R gene evolution and development. Allele mining for resistance genes can be an important method to identify additional resistance alleles and new haplotypes along with the development of allele-specific markers for use in marker-assisted selection. Allele mining can be visualised as a vital link between effective utilisation of genetic and genomic resources in genomics-driven modern plant breeding. This review studies the actual concepts and potential of mining approaches for the discovery of alleles and their utilisation for blast resistance genes in rice. The details provided here will be important to provide the rice breeder with a worthwhile introduction to allele mining and its methodology for breakthrough discovery of fresh alleles hidden in hereditary diversity, which is vital for crop improvement

The effect of hyperthyroidism on the levels of liver enzymes in adult male Wistar rats

 Thyrotoxicosis is a condition in which tissues are stimulated by increased secretion of thyroid hormone. The most common cause is diffuse toxic goiter and toxic multi-nodular goiter. For more reviews on this disease, the effects of hyperthyroidism on liver enzyme levels were studied. A total of 30 adult male Wistar rats weighing about 190 g were purchased from the Pasteur Institute of Iran. In this study, rats were divided into control group, the group receiving vitamin E, the group receiving levothyroxine, the group receiving levothyroxine treated with vitamin E; blood was taken from all groups over a period of 10 days after injection, and measurement of thyroid hormones and liver tests was made. The findings obtained in this study show that Isolated systolic hypertension (ISH) hormone levels in rats treated with levothyroxine, Treatment with vitamin E may reduce serum levels of ISH , Hormone levels of T4 in the rats treated with levothyroxine were increased compared to normal rates. Treatment with vitamin E reduces serum levels of T4 compared to the first hyper group. T4 hormone levels in rats treated with levothyroxine were reduced compared to normal rates. Treatment with vitamin E may reduce serum levels of T4 compared with the first hyper group. Asparagine Transferase (AST) enzyme levels in rats treated with levothyroxine were increased compared, Treatment with vitamin E may reduce serum levels of AST , Alanine transferase (ALT) enzyme levels in rats treated with levothyroxine were increased , Treatment with vitamin E may increase serum levels of ALT , alkaline phosphatase (ALP) enzyme levels in rats treated with levothyroxine has been increased compared with normal rates. Treatment with vitamin E resulted in serum levels of ALT not to be increased compared with the first group. According to the results of hyperthyroidism and levels of liver enzymes, it can be concluded that hyperthyroidism induced by levothyroxine can increase the levels of hormones T3, T4 and Thyroid Stimulating Hormone (TSH), and then increase the levels of liver enzymes. Treatment of empirical samples with vitamin E is likely to reduce liver damages and prevent the increased levels of liver enzymes compared to empirical samples of hyperthyroidism which have been treated with vitamin E.

Relationship between coping/attachment styles and infertility-specific distress in Iranian infertile individuals: A cross-sectional study

Background: Infertility has been recognized as a stressful clinical condition, significantly affecting couples’ emotional functioning. Objective: To investigate the relationship between coping/attachment styles and infertility-specific distress (ISD) in infertile participants. Materials and Methods: A total number of 240 infertile participants (120 women and 120 men) who attend the Outpatient Infertility Clinic in Sari, Iran between February and October 2017 were selected using the convenience sampling method. Data were collected using a questionnaire addressing sociodemographic variables. In addition, coping and attachment styles were evaluated via the Coping Strategies Questionnaire and the Revised Adult Attachment Scale by Collins and Read (1990); respectively. Ultimately, the Infertility Distress Scale was used to assess ISD. Results: The mean ISD score was 42.53 ± 9.63. Secure and insecure attachment styles were observed in 37.9% and 62.1% of the cases, respectively. There was a significant difference among ISD and different groups of attachment styles (p = 0.001) and emotion-focused coping style (p = 0.021). However, no significant relationship was found between problem-focused coping style and ISD (p = 0.985). Conclusion: Considering the relationship between coping/attachment styles and ISD, it was recommended to implement stress prevention and coping education within the framework of coping/attachment theories for infertile individuals. Key words: Infertility, Attachment, Copying, Distress

Hydro priming stimulates seedling growth and establishment of Malaysian Indica rice (MR219) under drought stress

Drought stress severely effects on seed germination and seedling establishment as critical stages in crops lifetime. Seed hydro priming is useful process for improving crops tolerance to drought stress. The present investigation was designed to evaluate the effect of hydro priming on adaptation strategies of Malaysian Indica rice (MR219) under drought conditions. Rice aseptic seeds were soaked at 20°C for 8h distilled water. Primed and non-primed seeds were subjected to polyethylene glycol (0, - 0.4, - 0.8 and -1.2) MPa treatments. Results showed that germination percentage, germination index, the fresh and dry weight, shoots and roots lengths decreased with increasing polyethylene glycol concentrations. We observed that polyethylene glycol tolerance of primed seeds is higher than non-primed seeds at all polyethylene glycol levels. Mean germination time and relative polyethylene glycol injury are in-creased in non-primed seeds as compare to primed seeds under drought stress. Proline content positively correlated increased with the increasing polyethylene glycol concentrations. The results indicated that hydro priming of Malaysian Indica rice (MR219) seeds is associated with the accumulation of proline and modulating the activity of ascorbate peroxidase and catalase under drought stress. This study suggests the hydro priming as an effective technique on rice seeds to withstand under drought condition which could be a step forward to commercialization

Isolation and expression analysis of novel silicon absorption gene from roots of mangrove (Rhizophora apiculata) via suppression subtractive hybridization

Silicon (Si) is the second most abundant element in soil after oxygen. It is not an essential element for plant growth and formation but plays an important role in increasing plant tolerance towards different kinds of abiotic and biotic stresses. The molecular mechanism of Si absorption and accumulation may differ between plants, such as monocotyledons and dicotyledons. Silicon absorption and accumulation in mangrove plants are affected indirectly by some proteins rich in serine and proline amino acids. The expression level of the genes responsible for Si absorption varies in different parts of plants. In this study, Si is mainly observed in the epidermal roots’ cell walls of mangrove plants compared to other parts. The present work was carried out to discover further information on Si stress responsive genes in Rhizophora apiculata, using the suppression subtractive hybridization technique. To construct the cDNA library, two-month-old seedlings were exposed to 0.5, 1, and 1.5 mM SiO2 for 15 hrs and for 1 to 6 days resulting in a total of 360 high quality ESTs gained. Further examination by RT-PCR and real-time qRT-PCR showed the expression of a candidate gene of serine-rich protein