Time-dependent changes in the brain arachidonic acid cascade during cuprizone-induced demyelination and remyelination

Phospholipases A(2) (PLA(2)) are the enzymatic keys for the activation of the arachidonic acid (AA) cascade and the subsequent synthesis of pro-inflammatory prostanoids (prostaglandins and tromboxanes). Prostanoids play critical roles in the initiation and modulation of inflammation and their levels have been reported increased in several neurological and neurodegenerative disorders, including multiple sclerosis (MS). Here, we aimed to determine whether brain expression PLA(2) enzymes and the terminal prostagland in levels are changed during cuprizone-induced demyelination and in the subsequent remyelination phase. Mice were given the neurotoxicant cuprizone through the diet for six weeks to induce brain demyelination. Then, cuprizone was withdrawn and mice were returned to a normal diet for 6 weeks to allow spontaneous remyelination. We found that after 4-6 weeks of cuprizone, sPLA(2)(V) and cPLA(2), but not iPLA(2)(VI), gene expression was upregulated in the cortex, concomitant with an increase in the expression of astrocyte and microglia markers. Cyclooxygenase (COX)-2 gene expression was consistently upregulated during all the demyelination period, whereas COX-1 sporadically increased only at week 5 of cuprizone exposure. However, we found that at the protein level only sPLA(2)(V) and COX-1 were elevated during demyelination, with COX-1 selectively expressed by activated and infiltrated microglia/macrophages and astrocytes. Levels of PGE(2), PGD(2), PGI(2) and TXB(2) were also increased during demyelination. During remyelination, none of the PLA(2) isoforms was significantly changed, whereas COX-1 and -2 were sporadically upregulated only at the gene expression level. PGE(2), PGI(2) and PGD(2) levels returned to normal, whereas TXB(2) was still upregulated after 3 weeks of cuprizone withdrawal. Our study characterizes for the first time time-dependent changes in the AA metabolic pathway during cuprizone-induced demyelination and the subsequent remyelination and suggests that sPLA(2)(V) is the major isoform contributing to AA release

Effects of municipal solid waste- and sewage sludge-compost-based growing media on the yield and heavy metal content of four lettuce cultivars

Compost has been recently suggested as an alternative to peat for the preparation of growing substrates in soilless cultivation systems. However, some physico-chemical properties of compost may reduce plant performance and endanger the quality of productions, in particular for possible heavy metal accumulation in edible parts. This study aims at evaluating the suitability of a municipal solid waste compost (MSWC) and a sewage sludge compost (SSC) as components of growing media for the soilless cultivation of lettuce (Lactuca sativa L.). Heavy metal content of SSC complied with legislation limits but, in MSWC, it exceeded (Cu, Pb) or was very close (Cd, Zn) to safe limits. A greenhouse experiment was carried out by cultivating four lettuce cultivars (“Maximus,” “Murai,” “Patagonia,” and “Aleppo”) in pots containing a mixture of MSWC and perlite (MSWC + P), SSC and perlite (SSC + P), or peat and perlite (peat + P), the latter used as control. Plant biometric parameters measured after 72 days of growth revealed that the yield of plants cultivated on SSC + P was similar to control plants, independently of the cultivar. Conversely, MSWC + P suppressed in general the biomass production, especially for Murai and Patagonia cultivars. Compared to peat + P, both compost-based substrates reduced the leaf accumulation of heavy metals, with a major effect in Maximus plants. The levels of Cd and Pb in the edible part were always below the safe limits imposed by European regulation. Therefore, risks of heavy metal intake in food chain associated with the replacement of peat with compost in the growing media are negligible, even when a compost with a significant amount of heavy metals is used. Besides compost quality monitoring, also an appropriate varietal choice is crucial to obtain good yields and safe products

Study of the middle ear behavior considering the presence of fluid

O objetivo deste trabalho foi estudar a influência de diferentes fluidos dentro e fora da cavidade timpânica no comportamento biomecânico do ouvido médio. O modelo computacional 3 D da cadeia tímpano‐ossicular foi construído através de imagens de tomografia axial computorizada (TAC). Após a construção do modelo da cadeia tímpano‐ossicular, para a parte interna da membrana timpânica foi criada a caixa timpânica, que inclui toda a cadeia ossicular, e para a parte externa da membrana foi criada uma passagem, simulando o canal auditivo externo. A discretização do modelo foi feita utilizando o método dos elementos finitos, com base no software ABAQUS. As propriedades mecânicas foram extraídas de trabalhos anteriores. Foram obtidos os deslocamentos do umbo e de um ponto central da platina do estribo, para um nível de pressão sonora de 105 dB aplicada na membrana timpânica, comparando a cadeia tímpano‐ossicular e os diferentes níveis de pressão externa e interna do ouvido médio (fluido líquido e fluido gasoso). Os resultados demonstram que o comportamento biomecânico do ouvido médio é diferente para os diferentes níveis de pressões testadas neste modelo. Quando utilizada uma pressão de fluido líquido, os deslocamentos são de amplitude inferior quando comparados com os resultados obtidos com pressão de ar.The objective of this work is to study the influence of different fluids of the tympanic cavity into the biomechanical behavior of the middle ear. The 3 D computer model of the tympanic ossicular chain was built using images from computed tomography (CT). After construction of the tympanic ossicular chain model, it was adapted the simulation by placing the passage to the external auditory canal and to the tympanic cavity. The discretization of the model was made using the finite element method based on ABAQUS software. The mechanical properties were extracted from previous works. The umbo and the stapes footplate displacements, for a sound pressure level of 105 dB applied in tympanic membrane were obtained, comparing the tympanic ossicular chain and the different levels of external and internal pressure in the middle ear (liquid and air). The results demonstrate that the biomechanical behavior of the middle ear is different for different levels of pressure tested in this model. When using liquid, displacements are of lower amplitude when compared with the results obtained with air.Peer Reviewe

The human otitis media with effusion: a numerical-based study

Otitis media is a group of inflammatory diseases of the middle ear. Acute otitis media and otitis media with effusion (OME) are its two main types of manifestation. Otitis media is common in children and can result in structural alterations in the middle ear which will lead to hearing losses. This work studies the effects of an OME on the sound transmission from the external auditory meatus to the inner ear. The finite element method was applied on the present biomechanical study. The numerical model used in this work was built based on the geometrical information obtained from The visible ear project. The present work explains the mechanisms by which the presence of fluid in the middle ear affects hearing by calculating the magnitude, phase and reduction of the normalized umbo velocity and also the magnitude and phase of the normalized stapes velocity. A sound pressure level of 90 dB SPL was applied at the tympanic membrane. The harmonic analysis was performed with the auditory frequency varying from 100 Hz to 10 kHz. A decrease in the response of the normalized umbo and stapes velocity as the tympanic cavity was filled with fluid was obtained. The decrease was more accentuated at the umbo.info:eu-repo/semantics/publishedVersio

Prediction, validation, and uncertainties of a nation‑wide post‑fire soil erosion risk assessment in Portugal

Wildfires are a recurrent and increasing threat in mainland Portugal, where over 4.5 million hectares of forests and scrublands have burned over the last 38 years. These fire-affected landscapes have suffered an intensification of soil erosion processes, which can negatively affect soil carbon storage, reduce fertility and forest productivity, and can become a source of pollutants. The main objective of the present study is to produce a post-fire soil erosion risk map for the forest and shrubland areas in mainland Portugal and assess its reliability. To this end, the semi-empirical Morgan–Morgan–Finney erosion model was used to assess the potential post-fire soil erosion according to distinct burn severity and climate scenarios, and the accuracy of the predictions was verified by an uncertainty analysis and validated against independent field datasets. The proposed approach successfully allowed mapping post-fire soil erosion in Portugal and identified the areas with higher post-fire erosion risk for past and future climate extremes. The outcomes of this study comprise a set of tools to help forest managers in their decision-making for post-fire emergency stabilization, ensuring the adequate selection of areas for mitigation to minimize the economic and environmental losses caused by fire-enhanced soil erosion.publishe

Oral platelet gel supernatant plus supportive medical treatment versus supportive medical treatment in the management of radiation-induced oral mucositis: a matched explorative active control trial by propensity analysis

OBJECTIVES:: In this active control trial, the rate of radio-induced WHO grade 3/4 oral mucositis and the change in quality of life, assessed by OMWQ-HN, were measured in subjects with head and neck cancer treated by platelet gel supernatant (PGS) and supportive medical treatment versus subjects treated by supportive medical treatment alone. MATERIALS AND METHODS:: Eighty patients with nonmetastatic head and neck cancer underwent curative or adjuvant radiotherapy. All patients underwent supportive medical treatment and/or PGS at the beginning and during radiotherapy. Sixteen patients received PGS in association with supportive medical treatment. To obtain 2 groups virtually randomized for important clinical characteristics subjects were matched, by propensity analysis, with a group of subjects (64 patients) treated with supportive medical treatment alone. RESULTS:: Subjects treated with standard supportive treatment experienced significant higher WHO grade 3/4 toxicity (55%; 35/64) than subjects treated by PGS (13%; 3/16). The reduced toxicity found in PGS group paralleled with the evidence that they developed later symptoms with respect to controls. The Cox proportional hazard model indicated that patients treated with standard supportive medical treatment experienced 2.7-fold increase (hazard ratio=2.7; 95% confidence interval, 1.3-5.7) in the occurrence of WHO grade 3/4 toxicity. PGS group significantly experienced higher quality of life than control groups as measured by OMWQ-HN. A significant decrease in the opioid analgesics usage was found in the PGS group. CONCLUSIONS:: These preliminary data should be interpreted with caution and could serve as a framework around which to design future trials

Pela construção de um repositório científico (temático) geológico

e-Terra - Revista Electrónica de Ciências da Terra ISSN 1645-0388, Volume 15 – nº 10 2010É apresentado o panorama actual, nacional e internacional, sobre o Acesso Livre ao conhecimento científico através da Web. São apresentadas estatísticas que demonstram os benefícios e a utilidade desta nova forma de divulgação do conhecimento, não apenas pelo incremento das citações, mas também pela formação dos jovens investigadores e, mesmo do ponto de vista ético. São também apresentadas estatísticas sobre as publicações periódicas de Geologia em Portugal, sobre as quais se conclui também pela necessidade da constituição de um repositório bibliográfico digital

Genetic characterization of the red algae Asparagopsis armata and Asparagopsis taxiformis (Bonnemaisoniaceae) from the Azores

info:eu-repo/semantics/publishedVersio