Hydrodynamical chemistry simulations of the SZ effect and the impacts from primordial non-Gaussianities

The impacts of Compton scattering of hot cosmic gas with the cosmic microwave background radiation (Sunyaev-Zel'dovich effect, SZ) are consistently quantified in Gaussian and non-Gaussian scenarios, by means of 3D numerical, N-body, hydrodynamic simulations, including cooling, star formation, stellar evolution and metal pollution (He, C, O, Si, Fe, S, Mg, etc.) from different stellar phases, according to proper yields for individual metal species and mass-dependent stellar lifetimes. Light cones are built through the simulation outputs and samples of one hundred maps for the resulting temperature fluctuations are derived for both Gaussian and non-Gaussian primordial perturbations. From them, we estimate the possible changes due to early non-Gaussianities on: SZ maps, probability distribution functions, angular power spectra and corresponding bispectra. We find that the different growth of structures in the different cases induces significant spectral distortions only in models with large non-Gaussian parameters, $f_{\rm NL}$. In general, the overall trends are covered by the non-linear, baryonic evolution, whose feedback mechanisms tend to randomize the gas behaviour and homogenize its statistical features, quite independently from the background matter distribution. Deviations due to non-Gaussianity are almost undistinguishable for $f_{\rm NL}\lesssim 100$, remaining always at few-per-cent level, within the error bars of the Gaussian scenario. Rather extreme models with $f_{\rm NL}\sim1000$ present more substantial deviations from the Gaussian case, overcoming baryon contaminations and showing discrepancies up to a factor of a few in the spectral properties.Comment: 10 pages, 4 figures, accepted for publication on MNRA

Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

BACKGROUND: We describe an ELISA-based method that can be used to identify and quantitate proteins in biological samples. In this method, peptides in solution, derived from proteolytic digests of the sample, compete with substrate-attached synthetic peptides for antibodies, also in solution, generated against the chosen peptides. The peptides used for the ELISA are chosen on the basis of their being (i) products of the proteolytic (e.g. tryptic) digestion of the protein to be identified and (ii) unique to the target protein, as far as one can know from the published sequences. RESULTS: In this paper we describe the competition assay and we define the optimal conditions for the most effective assay. We have performed an analysis of the kinetics of interaction between the four components of the assay: the plastic substratum to which the peptide is bound, the bound peptide itself, the competing added peptide, and the antibody that is specific for the peptide and we compare the results of theoretical simulations to the actual data in some model systems. CONCLUSION: The data suggest that the peptides bind to the plastic substratum in more than one conformation and that, once bound, the peptide displays different affinities for the antibody, depending on how it has bound to the plat

An ELISA-based procedure for assaying proteins in digests of human leukocytes and cell lines, using specifically selected peptides and appropriate antibodies

BACKGROUND: We describe the application of an ELISA-based assay (the Peptidomatrix) that can be used to simultaneously identify and quantitate a number of proteins in biological samples. The biological sample (blood component, biopsy, culture or other) is first lysed to release all the proteins, without any additional separation. The denatured proteins in the sample are then digested in bulk with the desired proteolytic enzyme(s). The peptides in the digest are then assayed by appropriate antibodies, using a competition ELISA protocol. RESULTS: As an example of its use, the present paper applies the Peptidomatrix to the assay of four membrane proteins MDR1 (P-glycoprotein or ABCB1), MRP1 (ABCC1), BCRP/MXR (ABCG2) and the alpha subunit of the Na, K_ATPase (ATP1A1), present in a number of cell lines and in human lymphocytes. We show that we can detect and quantitate these proteins, using a series of peptide-antibody pairs, and that we can differentiate between cell lines or cell preparations that express the target proteins and those that do not. CONCLUSION: We have devised a simple, ELISA-based proteomics assay that enables the quantitation of designated proteins in a cell or tissue sample, and that can be used in any laboratory, with minimal specialized equipment

T Lymphocyte Subset Counts and Interferon-Gamma Production in Adults and Children with COVID-19: A Narrative Review

Adults and children exhibit a broad range of clinical outcomes from SARS-CoV-2 infection, with minimal to mild symptoms, especially in the pediatric age. However, some children present with a severe hyperinflammatory post-infectious complication named multisystem inflammatory syndrome in children (MIS-C), mainly affecting previously healthy subjects. Understanding these differences is still an ongoing challenge, that can lead to new therapeutic strategies and avoid unfavorable outcomes. In this review, we discuss the different roles of T lymphocyte subsets and interferon-? (IFN-?) in the immune responses of adults and children. Lymphopenia can influence these responses and represent a good predictor for the outcome, as reported by most authors. The increased IFN-? response exhibited by children could be the starting point for the activation of a broad response that leads to MIS-C, with a significantly higher risk than in adults, although a single IFN signature has not been identified. Multicenter studies with large cohorts in both age groups are still needed to study SARS-CoV-2 pathogenesis with new tools and to understand how is possible to better modulate immune responses

Promoting Laparoscopic Anterior Approach for a Very Low Presacral Primary Neuroendocrine Tumor Arising in a Tailgut Cyst

Tailgut cysts are rare congenital lesions that develop in the presacral space. As they can potentially conceal primary neuroendocrine tumors, surgical excision is suggested as the treatment of choice. However, specific management guidelines have yet to be developed. A posterior approach is usually preferred for cysts extending to the third sacral vertebral body. Conversely, a transabdominal approach is preferred for lesions extending upward to achieve an optimal view of the surgical field and avoid injuries

Baryon history and cosmic star formation in non-Gaussian cosmological models: numerical simulations

We present the first numerical, N-body, hydrodynamical, chemical simulations of cosmic structure formation in the framework of non-Gaussian models. We study the impact of primordial non-Gaussianities on early chemistry (e, H, H+, H-, He, He+, He++, H2, H2+, D, D+, HD, HeH+), molecular and atomic gas cooling, star formation, metal (C, O, Si, Fe, Mg, S) enrichment, population III (popIII) and population II-I (popII) transition, and on the evolution of "visible" objects. We find that non-Gaussianities can have some consequences on baryonic structure formation at very early epochs, but the subsequent evolution at later times washes out any difference among the various models. When assuming reasonable values for primordial non-Gaussian perturbations, it turns out that they are responsible for: (i) altering early molecular fractions in the cold, dense gas phase of ~10 per cent; (ii) inducing small temperature fluctuations of <~10 per cent during the cosmic evolution of primordial objects; (iii) influencing the onset of the first star formation events, at z>~15, and of the popIII/popII transition of up to some 10^7yr; (iv) determining variations of <~10 per cent in the gas cloud and stellar mass distributions after the formation of the first structures; (v) causing only mild variations in the chemical history of the Universe. We stress, though, that purely non-Gaussian effects might be difficult to address, since they are strictly twisted with additional physical phenomena (e.g. primordial gas bulk flows, unknown primordial popIII stellar mass function, etc.) that have similar or stronger impact on the behaviour of the baryons.Comment: Accepted for publications on MNRAS, on April 13, 2011. Minor revision

The electrical design of the thermal control systems of the all-aluminum ARIEL telescope

The Atmospheric Remote-sensing InfraRed Large-survey (ARIEL) is a medium-class mission of the European Space Agency whose launch is planned by late 2029 whose aim is to study the composition of exoplanet atmospheres, their formation and evolution. The ARIEL’s target will be a sample of about 1000 planets observed with one or more of the following methods: transit, eclipse and phase-curve spectroscopy, at both visible and infrared wavelengths simultaneously. The scientific payload is composed by a reflective telescope having a 1m-class primary mirror, built in solid aluminum, and two focal-plane instruments: 1. FGS (Fine Guidance System), performing photometry in visible light and low resolution spectrometry over three bands (from 0.8 to 1.95 µm) 2. AIRS (ARIEL InfraRed Spectrometer) that will perform infrared spectrometry in two wavelength ranges between 1.95 and 7.8 µm. This paper depicts the status of the TA (Telescope Assembly) electric section whose purpose is to deploy sensors, managed by the Telescope Control Unit, for the precise monitoring of the Telescope’s temperatures and the decontamination system, used to avoid the contamination of the optical surfaces (mirrors in primis)

Psychological treatments and psychotherapies in the neurorehabilitation of pain. Evidences and recommendations from the italian consensus conference on pain in neurorehabilitation

BACKGROUND: It is increasingly recognized that treating pain is crucial for effective care within neurological rehabilitation in the setting of the neurological rehabilitation. The Italian Consensus Conference on Pain in Neurorehabilitation was constituted with the purpose identifying best practices for us in this context. Along with drug therapies and physical interventions, psychological treatments have been proven to be some of the most valuable tools that can be used within a multidisciplinary approach for fostering a reduction in pain intensity. However, there is a need to elucidate what forms of psychotherapy could be effectively matched with the specific pathologies that are typically addressed by neurorehabilitation teams. OBJECTIVES: To extensively assess the available evidence which supports the use of psychological therapies for pain reduction in neurological diseases. METHODS: A systematic review of the studies evaluating the effect of psychotherapies on pain intensity in neurological disorders was performed through an electronic search using PUBMED, EMBASE, and the Cochrane Database of Systematic Reviews. Based on the level of evidence of the included studies, recommendations were outlined separately for the different conditions. RESULTS: The literature search yielded 2352 results and the final database included 400 articles. The overall strength of the recommendations was medium/low. The different forms of psychological interventions, including Cognitive-Behavioral Therapy, cognitive or behavioral techniques, Mindfulness, hypnosis, Acceptance and Commitment Therapy (ACT), Brief Interpersonal Therapy, virtual reality interventions, various forms of biofeedback and mirror therapy were found to be effective for pain reduction in pathologies such as musculoskeletal pain, fibromyalgia, Complex Regional Pain Syndrome, Central Post-Stroke pain, Phantom Limb Pain, pain secondary to Spinal Cord Injury, multiple sclerosis and other debilitating syndromes, diabetic neuropathy, Medically Unexplained Symptoms, migraine and headache. CONCLUSIONS: Psychological interventions and psychotherapies are safe and effective treatments that can be used within an integrated approach for patients undergoing neurological rehabilitation for pain. The different interventions can be specifically selected depending on the disease being treated. A table of evidence and recommendations from the Italian Consensus Conference on Pain in Neurorehabilitation is also provided in the final part of the pape

Protected silver coating for Ariel telescope mirrors: study of ageing effects

The Atmospheric Remote-sensing Infrared Exoplanet Large-survey (Ariel), selected as ESA’s fourth mediumclass mission in the Cosmic Vision program, is set to launch in 2029. The objective of the study is to conduct spectroscopic observations of approximately one thousand exoplanetary atmospheres for better understanding the planetary system formation and evolution and identifying a clear link between the characteristics of an exoplanet and those of its parent star. The realization of the Ariel’s telescope is a challenging task that is still ongoing. It is an off-axis Cassegrain telescope (M1 parabola, M2 hyperbola) followed by a re-collimating off-axis parabola (M3) and a plane fold mirror (M4). It is made of Al 6061 and designed to operate at visible and infrared wavelengths. The mirrors of the telescope will be coated with protected silver, qualified to operate at cryogenic temperatures. The qualification of the coating was performed according to the ECSS Q-ST-70-17C standard, on a set of samples that have been stored in ISO 6 cleanroom conditions and are subjected to periodic inspection and reflectance measurements to detect any potential performance degradation. The samples consist of a set of Aluminum alloy Al 6061-T651 disks coated with protected silver. This paper presents the results of the morphological characterization of the samples based on Atomic Force Microscopy (AFM) and the reflectivity measurement in the infrared by Fourier Transform Infrared (FTIR) spectroscopy

Planning the integration and test of a space telescope with a 1 m aluminum primary mirror: the Ariel mission case

Ariel (Atmospheric Remote-Sensing Infrared Exoplanet Large Survey) is ESA’s M4 mission of the “Cosmic Vision” program, with launch scheduled for 2029. Its purpose is to conduct a survey of the atmospheres of known exoplanets through transit spectroscopy. Ariel is based on a 1 m class telescope optimized for spectroscopy in the waveband between 1.95 and 7.8 µm, operating at cryogenic temperatures in the range 40–50 K. The Ariel Telescope is an off-axis, unobscured Cassegrain design, with a parabolic recollimating tertiary mirror and a flat folding mirror directing the output beam parallel to the optical bench. The secondary mirror is mounted on a roto-translating stage for adjustments during the mission. The mirrors and supporting structures are all realized in an aerospace-grade aluminum alloy T6061 for ease of manufacturing and thermalization. The low stiffness of the material, however, poses unique challenges to integration and alignment. Care must be therefore employed when designing and planning the assembly and alignment procedures, necessarily performed at room temperature and with gravity, and the optical performance tests at cryogenic temperatures. This paper provides a high-level description of the Assembly, Integration and Test (AIT) plan for the Ariel telescope and gives an overview of the analyses and reasoning that led to the specific choices and solutions adopted