Selective Functionalization of Arene C(sp2 )−H Bonds by Gold Catalysis: The Role of Carbene Substituents

The complete regioselective incorporation of carbene units to nonactivated arene rings has been achieved employing gold(I) catalysts bearing alkoxydiaminophosphine ligands, with readily available, nonelaborated ethyl 2-phenyl-diazoacetate as the carbene source. These results are in contrast with the scarce precedents which required highly elaborated diazo substrates. Density functional theory (DFT) calculations have revealed the important role of the R group in the C(R)CO 2Et fragment, which dramatically affects the energy profile of this transformation.We thank the Ministerio de Ciencia e Innovación for Grant PID2020-113797RB-C21, also financed by FEDER “Una manera de hacer Europa”. We also thank Junta de Andalucía (P18-RT-1536) and Universidad de Huelva (P.O. Feder UHU-1260216). I.S. and A.N. acknowledge the support from the Research Council of Norway through its FRINATEK (No. 314321), Centre of Excellence schemes (No. 262695), and the Norwegian Metacenter for Computational Science (NOTUR) for computational resources (project number nn4654k). Funding for open access charge: Universidad de Huelva / CBU

Speleoseismology and palaeoseismicity of Benis Cave (Murcia, SE Spain): coseismic effects of the 1999 Mula earthquake (mb 4.8)

This work describes the coseismic ceiling block collapse within Benis Cave (−213 m; Murcia, SE Spain), associated with the 1999 Mula earthquake (mb=4.8, MSK VII). The collapse occurred at −156 m into the Earthquake Hall, and as a consequence one small gallery became blind. We studied the geology, topography and active tectonic structures relevant to the cave. In addition, we carried out a seismotectonic analysis of the focal mechanism solutions, and also a fault population analysis on slickensides measured in fault planes in the cave. The stress and strain regime is interpreted as being congruent with the palaeoseismic evidence, and agrees with the fault kinematics established for cave galleries developed within fault planes and growth anomalies of coral flowstone. Our analysis suggests that one active segment (NNE–SSW) determined the morphology and topography of the Benis Cave, where strong to moderate palaeoearthquakes (6≤M≤7) took place. As a consequence of this intense seismic activity a small gallery collapsed. A new palaeoseismic structure, or seismothem, has been recognized, namely the effect of palaeoearthquakes affecting the pattern of development of the spatial coral flowstone distribution located at the bottom of the cave

An Intelligent Architecture Based on Field Programmable Gate Arrays Designed to Detect Moving Objects by Using Principal Component Analysis

This paper presents a complete implementation of the Principal Component Analysis (PCA) algorithm in Field Programmable Gate Array (FPGA) devices applied to high rate background segmentation of images. The classical sequential execution of different parts of the PCA algorithm has been parallelized. This parallelization has led to the specific development and implementation in hardware of the different stages of PCA, such as computation of the correlation matrix, matrix diagonalization using the Jacobi method and subspace projections of images. On the application side, the paper presents a motion detection algorithm, also entirely implemented on the FPGA, and based on the developed PCA core. This consists of dynamically thresholding the differences between the input image and the one obtained by expressing the input image using the PCA linear subspace previously obtained as a background model. The proposal achieves a high ratio of processed images (up to 120 frames per second) and high quality segmentation results, with a completely embedded and reliable hardware architecture based on commercial CMOS sensors and FPGA devices

Identificación y caracterización de genes implicados en la maduración y la calidad de la fresa

Ripening is a critical step for the development of flavor quality in fruits. This character has significantly declined in many fleshy fruits over recent decades. This is particularly significant in strawberry (Fragaria × ananassa), where current cultivars are derived from a narrow germplasm collection. Improving fruit quality requires two important breakthroughs: 1) a precise understanding of the fruit ripening process that will allow the targeting of relevant genes, and 2) the identification of novel alleles responsible for fruit quality traits. In our project (TRANSFR-Q, Starting Grant-ERC), we aim at the identification and characterization of key transcription factors involved in fruit ripening regulation and their target genes, in order to infer the Gene Regulatory Network controlling this process. On the other hand, we are carrying out a Genome-Wide Association Study using a germplasm collection of the woodland strawberry (Fragaria vesca) in order to identify loci involved in important traits such as aroma, fruit size, and resistance to pathogens. Finally, we have implemented the use of the genome-editing tool CRISPR/Cas9 in the cultivated strawberry, which we expect to open opportunities for engineering this species to improve traits of economic importance.ERC Starting Grant ERC-2014-StG 63813

Effects of the herbicide Roundup on freshwater microbial communities: a mesocosm study

The impact of the widely used herbicide glyphosate has been mainly studied in terrestrial weed control, laboratory bioassays, and field studies focusing on invertebrates, amphibians, and fishes. Despite the importance of phytoplankton and periphyton communities at the base of the aquatic food webs, fewer studies have investigated the effects of glyphosate on freshwater microbial assemblages. We assessed the effect of the commercial formulation Roundup using artificial earthen mesocosms. The herbicide was added at three doses: a control (without Roundup) and two treatments of 6 and 12 mg/L of the active ingredient (glyphosate). Estimates of the dissipation rate (k) were similar in the two treatments (half-lives of 5.77 and 7.37 d, respectively). The only two physicochemical parameters showing statistically significant differences between treatments and controls were the downward vertical spectral attenuation coefficient kd(k), where k is wavelength, and total phosphorus concentration (TP). At the end of the experiment, the treated mesocosms showed a significant increase in the ratio kd(490 nm)/kd(550 nm) and an eightfold increase in TP. Roundup affected the structure of phytoplankton and periphyton assemblages. Total micro- and nanophytoplankton decreased in abundance in treated mesocosms. In contrast, the abundance of picocyanobacteria increased by a factor of about 40. Primary production also increased in treated mesocosms (roughly by a factor of two). Similar patterns were observed in the periphytic assemblages, which showed an increased proportion of dead : live individuals and increased abundances of cyanobacteria (about 4.5-fold). Interestingly, the observed changes in the microbial assemblages were captured by the analysis of the pigment composition of the phytoplankton, the phytoplankton absorption spectra, and the analysis of the optical properties of the water. The observed changes in the structure of the microbial assemblages are more consistent with a direct toxicological effect of glyphosate rather than an indirect effect mediated by phosphorus enrichment.Facultad de Ciencias Naturales y Muse

Active tectonics on a volcanic island by using fractal and structural techniques (Deception Island, West Antarctica)

En este trabajo se obtiene el campo de esfuerzo y deformación activo en la isla volcánica de Decepción, en la Antártida Occidental. Para ello, se han aplicado tres técnicas estructurales diferentes: (1) medida de planos de falla con vector de deslizamiento, (2) estudio de la geometría fractal de la distribución espacial de lineamientos en la isla, interpretados a partir de imágenes satélite y MDT de alta resolución y (3) cartografía y geometría de la caldera volcánica. El campo de deformación obtenido es de tipo extensional con dos orientaciones ortogonales para el máximo acortamiento horizontal (ey), NE-SO y NO-SE. El campo de esfuerzos obtenido mediante el análisis fractal y de la geometría de la caldera volcánica se define a partir de SHMAX según NE-SO.The tectonic field on Deception Island (South Shetlands, West Antarctica) is determined from structural and fractal analyses. Three different analyses are applied to the study of the strain and stress fields into the area: (1) field measurements of faults (strain analysis), (2) fractal geometry of the spatial distribution of lineaments and (3) the caldera plan shape (stress analysis). In this work, the identified strain field is extensional with the maximum horizontal shortening trending NE-SW and NW-SE. The fractal technique applied to the spatial distribution of lineaments indicates a stress field with SHMAX oriented NE-SW. The elliptical caldera of Deception Island, determined from field mapping, satellite imagery and vents and fissure eruptions, has an elongate shape and a stress field with SHMAX trending NE-SW as well.Depto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEMinisterio de Ciencia e Innovación (MICINN)pu

Rationale and design of the pragmatic clinical trial tREatment with Beta-blockers after myOcardial infarction withOut reduced ejection fracTion (REBOOT).

There is a lack of evidence regarding the benefits of β-blocker treatment after invasively managed acute myocardial infarction (MI) without reduced left ventricular ejection fraction (LVEF). The tREatment with Beta-blockers after myOcardial infarction withOut reduced ejection fracTion (REBOOT) trial is a pragmatic, controlled, prospective, randomized, open-label blinded endpoint (PROBE design) clinical trial testing the benefits of β-blocker maintenance therapy in patients discharged after MI with or without ST-segment elevation. Patients eligible for participation are those managed invasively during index hospitalization (coronary angiography), with LVEF >40%, and no history of heart failure (HF). At discharge, patients will be randomized 1:1 to β-blocker therapy (agent and dose according to treating physician) or no β-blocker therapy. The primary endpoint is a composite of all-cause death, non-fatal reinfarction, or HF hospitalization over a median follow-up period of 2.75 years (minimum 2 years, maximum 3 years). Key secondary endpoints include the incidence of the individual components of the primary composite endpoint, the incidence of cardiac death, and incidence of malignant ventricular arrhythmias or resuscitated cardiac arrest. The primary endpoint will be analysed according to the intention-to-treat principle. The REBOOT trial will provide robust evidence to guide the prescription of β-blockers to patients discharged after MI without reduced LVEF.REBOOT is a non-commercial trial whose main sponsor is the Spanish National Center for Cardiovascular Research (CNIC). The study also received partial funding from the BI group through the CIBERCV network.S

Neuropathological findings in fatal COVID-19 and their associated neurological clinical manifestations

9 p.Severe cases of Coronavirus Disease 2019 (COVID-19) can present with multiple neurological symptoms. The available neuropathological studies have described different lesions; the most frequent was the presence of neuroinflammation and vascular-related lesions. The objective of this study was to report the neuropathological studies performed in a medical institution, with abundant long intensive care unit stays, and their associated clinical manifestations. This is a retrospective monocentric case series study based on the neuropathological reports of 13 autopsies with a wide range of illness duration (13-108 days). A neuroinflammatory score was calculated based on the quantification of CD8- and CD68-positive cells in representative areas of the central nervous system. This score was correlated afterwards with illness duration and parameters related to systemic inflammation. Widespread microglial and cytotoxic T-cell activation was found in all patients. There was no correlation between the neuroinflammatory score and the duration of the illness; nor with parameters of systemic inflammation such as the peak of IL-6 or the HScore (a parameter of systemic macrophage activation syndrome). Two patients had global hypoxic ischaemic damage and five patients had subacute infarcts. One patient had many more brain vascular microthrombi compared to the others and multiple subacute pituitary infarcts. SARS-CoV-2 RNA was not detected with qRT-PCR. The proportion of brain lesions in severe COVID-19 patients could be related to illness duration. In our series, with abundant long hospitalisation stays, neuroinflammation was present in all patients and was more prominent between day 34 and day 45 after onset of symptoms. Clinical correlation showed that two patients with the highest neuroinflammatory scores had severe encephalopathies that were not attributable to any other cause. The second most frequent lesions were related to vascular pathology.Instituto de Salud Carlos IIICIBERONCInstituto Ramón y Cajal de Investigación SanitariaMerck, Sharp & Dohme (MSD

Collective Effervescence, Self-Transcendence, and Gender Differences in Social Well-Being During 8 March Demonstrations

8 March (8M), now known as International Women’s Day, is a day for feminist claims where demonstrations are organized in over 150 countries, with the participation of millions of women all around the world. These demonstrations can be viewed as collective rituals and thus focus attention on the processes that facilitate different psychosocial effects. This work aims to explore the mechanisms (i.e., behavioral and attentional synchrony, perceived emotional synchrony, and positive and transcendent emotions) involved in participation in the demonstrations of 8 March 2020, collective and ritualized feminist actions, and their correlates associated with personal well-being (i.e., affective well-being and beliefs of personal growth) and collective well-being (i.e., social integration variables: situated identity, solidarity and fusion), collective efficacy and collective growth, and behavioral intention to support the fight for women’s rights. To this end, a cross-cultural study was conducted with the participation of 2,854 people (age 18–79; M = 30.55; SD = 11.66) from countries in Latin America (Mexico, Chile, Argentina, Brazil, Peru, Colombia, and Ecuador) and Europe (Spain and Portugal), with a retrospective correlational cross-sectional design and a convenience sample. Participants were divided between demonstration participants (n = 1,271; 94.0% female) and non-demonstrators or followers who monitored participants through the media and social networks (n = 1,583; 75.87% female). Compared with non-demonstrators and with males, female and non-binary gender respondents had greater scores in mechanisms and criterion variables. Further random-effects model meta-analyses revealed that the perceived emotional synchrony was consistently associated with more proximal mechanisms, as well as with criterion variables. Finally, sequential moderation analyses showed that proposed mechanisms successfully mediated the effects of participation on every criterion variable. These results indicate that participation in 8M marches and demonstrations can be analyzed through the literature on collective rituals. As such, collective participation implies positive outcomes both individually and collectively, which are further reinforced through key psychological mechanisms, in line with a Durkheimian approach to collective rituals.Fil: Zumeta, Larraitz N.. Universidad del País Vasco; EspañaFil: Castro Abril, Pablo. Universidad del País Vasco; EspañaFil: Méndez, Lander. Universidad del País Vasco; EspañaFil: Pizarro, José J.. Universidad del País Vasco; EspañaFil: Włodarczyk, Anna. Universidad Católica del Norte; ChileFil: Basabe, Nekane. Universidad del País Vasco; EspañaFil: Navarro Carrillo, Ginés. Universidad de Jaén; EspañaFil: Padoan De Luca, Sonia. Universidad del País Vasco; EspañaFil: da Costa, Silvia. Universidad del País Vasco; EspañaFil: Alonso Arbiol, Itziar. Universidad del País Vasco; EspañaFil: Torres Gómez, Bárbara. Universidad del País Vasco; EspañaFil: Cakal, Huseyin. Keele University; Reino UnidoFil: Delfino, Gisela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Techio, Elza M.. Universidade Federal da Bahia; BrasilFil: Alzugaray, Carolina. Universidad de Santo Tomas; ChileFil: Bilbao, Marian. Universidad Alberto Hurtado; ChileFil: Villagrán, Loreto. Universidad de Concepción; ChileFil: López López, Wilson. Pontificia Universidad Javeriana; ColombiaFil: Ruiz Pérez, José Ignacio. Universidad Nacional de Colombia; ColombiaFil: Cedeño, Cynthia C.. Universidad Politécnica Salesiana; EcuadorFil: Reyes Valenzuela, Carlos. Universidad Andina Simon Bolivar - Sede Ecuador.; EcuadorFil: Alfaro Beracoechea, Laura. Universidad de Guadalajara; MéxicoFil: Contreras Ibáñez, Carlos César. Universidad Autónoma Metropolitana; MéxicoFil: Ibarra, Manuel Leonardo. Universidad Autónoma del Estado de México; MéxicoFil: Reyes Sosa, Hiram. Universidad Autonoma de Coahuila; MéxicoFil: Cueto, Rosa María. Pontificia Universidad Católica de Perú; PerúFil: Carvalho, Catarina L.. Universidad de Porto; PortugalFil: Pinto, Isabel R.. Universidad de Porto; Portuga