4,190 research outputs found

    Mixture enhances productivity in a two-species forest: evidence from a modeling approach

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    The effect of mixture on productivity has been widely studied for applications related to agriculture but results in forestry are scarce due to the difficulty of conducting experiments. Using a modeling approach, we analyzed the effect of mixture on the productivity of forest stands composed of sessile oak and Scots pine. To determine whether mixture had a positive effect on productivity and if there was an optimum mixing proportion, we used an aggregation technique involving a mean-field approximation to analyze a distance-dependent individual-based model. We conducted a local sensitivity analysis to identify the factors that influenced the results the most. Our model made it possible to predict the species proportion where productivity peaks. This indicates that transgressive over-yielding can occur in these stands and suggests that the two species are complementary. For the studied growth period, mixture does have a positive effect on the productivity of oakpine stands. Depending on the plot, the optimum species proportion ranges from 38 to 74% of oak and the gain in productivity compared to the current mixture is 2.2% on average. The optimum mixing proportion mainly depends on parameters concerning intra-specific oak competition and yet, intra-specific competition higher than inter-specific competition was not sufficient to ensure over-yielding in these stands. Our work also shows how results obtained for individual tree growth may provide information on the productivity of the whole stand. This approach could help us to better understand the link between productivity, stand characteristics, and species growth parameters in mixed forests

    La résistance induite : une nouvelle stratégie de défense des plantes contre les agents pathogènes

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    Tout au long de leur co-évolution, les plantes et les microorganismes pathogènes ont développé des relations complexes résultant d'un échange constant d'informations moléculaires. Les agents pathogènes ont élaboré toute une gamme de stratégies offensives pour parasiter les plantes et en contrepartie, les plantes ont déployé un arsenal défensif similaire à bien des égards aux défenses immunitaires animales. Les percées récentes en biologie moléculaire et en transformation des végétaux ont démontré que sensibiliser une plante à répondre plus rapidement à l'infection pouvait lui conférer une protection accrue contre des microorganismes virulents. Un aspect important dans la mise en évidence du rôle joué par les molécules de défense au niveau de l'expression de la résistance est une connaissance exacte de leur localisation spatio-temporelle dans les tissus en état de stress. Afin de cerner le processus associé à l'induction de résistance chez les plantes, l'effet d'éliciteurs biologiques, microbiens et chimiques sur la réponse cellulaire des plantes envers une attaque pathogène a fait l'objet d'investigations et les mécanismes impliqués dans le phénomène ont été étudiés. Dans tous les cas, il a été montré qu'une corrélation existait entre la réponse globale de la plante et des changements dans la biochimie et la physiologie des cellules, lesquels étaient accompagnés de modifications structurales incluant la formation d'appositions pariétales riches en callose et l'infiltration de composés phénoliques aux sites de pénétration potentielle par l'agent pathogène. L'activation du sentier des phénylpropanoïdes est un phénomème crucial dans la restriction de la croissance de l'agent pathogène et dans la survie des cellules-hôtes en conditions de stress. Bien qu'il n'existe que peu d'exemples d'application pratique de la résistance induite en tant que méthode de lutte contre les maladies des plantes, les résultats obtenus à partir de quelques expériences menées en plein champ et en serre sont encourageants et indiquent que cette approche a le potentiel de devenir une stratégie de lutte efficace et durable contre toute une gamme d'agents pathogènes.During the course of their coevolution, plants and pathogens have evolved an intricate relationship resulting from a continuous exchange of molecular information. Pathogens have developed an array of offensive strategies to parasitize plants and, in turn, plants have deployed a wide range of defense mechanisms similar in some respects to the immune defenses produced in animais. The recent advances in molecular biology and plant transformation have provided evidence that sensitizing a plant to respond more rapidly to infection could confer increased protection against virulent pathogens. One important facet in ascertaining the significance of defense molecules in plant disease resistance isthe exact knowledgeof their spatio-temporal distribution in stressed plant tissues. In an effort to understand the process associated with the induction of plant disease resistance, the effect of biological, microbial and chemical elicitors on the plant cell response during attack by fungal pathogens was investigated and the mechanisms underlying the expression of resistance studied. Evidence was provided that, in all cases, disease-resistance reactions correlated with changes in cell biochemistry and physiology that were accompanied by structural modifications including the formation of callose-enriched wall appositions and the infiltration of phenolic compounds at sites of potential pathogen penetration. Activation of the phenylpropanoid pathway appeared to be a crucial phenomenon involved in pathogen growth restriction and host cell survival under stress conditions. Although examples of practical use of induced resistance as a method of plant disease control are few, a number of field and greenhouse experiments are encouraging and indicate that this approach has the potential to become a powerful strategy against an array of pathogens in a persistent manner

    Water Vapour Effects in Mass Measurement

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    Water vapour inside the mass comparator enclosure is a critical parameter. In fact, fluctuations of this parameter during mass weighing can lead to errors in the determination of an unknown mass. To control that, a proposal method is given and tested. Preliminary results of our observation of water vapour sorption and desorption processes from walls and mass standard are reported

    Discovery and Selection of Certified Web Services Through Registry-Based Testing and Verification

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    Reliability and trust are fundamental prerequisites for the establishment of functional relationships among peers in a Collaborative Networked Organisation (CNO), especially in the context of Virtual Enterprises where economic benefits can be directly at stake. This paper presents a novel approach towards effective service discovery and selection that is no longer based on informal, ambiguous and potentially unreliable service descriptions, but on formal specifications that can be used to verify and certify the actual Web service implementations. We propose the use of Stream X-machines (SXMs) as a powerful modelling formalism for constructing the behavioural specification of a Web service, for performing verification through the generation of exhaustive test cases, and for performing validation through animation or model checking during service selection

    Constitutive association of BRCA1 and c-Abl and its ATM-dependent disruption after irradiation

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    BRCA1 plays an important role in mechanisms of response to double-strand breaks, participating in genome surveillance, DNA repair, and cell cycle checkpoint arrests. Here, we identify a constitutive BRCA1-c-Abl complex and provide evidence for a direct interaction between the PXXP motif in the C terminus of BRCA1 and the SH3 domain of c-Abl. Following exposure to ionizing radiation (IR), the BRCA1-c-Abl complex is disrupted in an ATM-dependent manner, which correlates temporally with ATM-dependent phosphorylation of BRCA1 and ATM-dependent enhancement of the tyrosine kinase activity of c-Abl. The BRCA1-c-Abl interaction is affected by radiation-induced modification to both BRCA1 and c-Abl. We show that the C terminus of BRCA1 is phosphorylated by c-Abl in vitro. In vivo, BRCA1 is phosphorylated at tyrosine residues in an ATM-dependent, radiation-dependent manner. Tyrosine phosphorylation of BRCA1, however, is not required for the disruption of the BRCA1-c-Abl complex. BRCA1-mutated cells exhibit constitutively high c-Abl kinase activity that is not further increased on exposure to IR. We suggest a model in which BRCA1 acts in concert with ATM to regulate c-Abl tyrosine kinase activity

    Functional equations and information measures with preference

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    H(3)+ correlators from Liouville theory

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    We prove that arbitrary correlation functions of the H(3)+ model on a sphere have a simple expression in terms of Liouville theory correlation functions. This is based on the correspondence between the KZ and BPZ equations, and on relations between the structure constants of Liouville theory and the H(3)+ model. In the critical level limit, these results imply a direct link between eigenvectors of the Gaudin Hamiltonians and the problem of uniformization of Riemann surfaces. We also present an expression for correlation functions of the SL(2)/U(1) coset model in terms of correlation functions in Liouville theory.Comment: 24 pages, v3: minor changes, references adde

    Self-Assembly of an Organized Cementum-Periodontal Ligament-Like Complex Using Scaffold-Free Tissue Engineering

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    A major challenge in regenerating periodontal tissues is emulating its complex structure containing both mineralized and soft tissues. In this study, scaffold-free tissue constructs engineered using periodontal ligament cells (PDLCs), which contain a population of adult stem/progenitor cells, self-assembled into an organized multi-tissue structure comprising a mineralized cementum-like core enclosed within a periodontal ligament (PDL)-like tissue. Scaffold-free engineered constructs were formed by culturing human PDLCs to form a cell sheet on six-well dishes containing two minutien pins placed 7 mm apart. The cell sheet was contracted by the cells to roll into the pins forming a cylindrical construct anchored on either end by the pins. These tissues were approximately 1 mm in diameter and 7 mm long and contained only the cells and their endogenous matrix. These scaffold-free engineered constructs exhibited two structurally distinct tissues, one in the center of the construct and another on the periphery. The center tissue was mineralized and expressed alkaline phosphatase and bone sialoprotein, similar to cementum. The peripheral tissue was not calcified and expressed periodontal ligament-associated protein-1 and periostin, which is characteristic of the periodontal ligament. This tissue organization was seen after in vitro culture and maintained in vivo following subcutaneous implantation in immunocompromised mice. These data demonstrate that scaffold-free tissue engineering facilitates PDLCs to self-assemble into an organized cementum-PDL-like complex. These engineered tissues could be used as implantable grafts to regenerate damaged periodontal tissues or as model systems to study PDLC biology and mechanisms driving organized tissue assembly within the periodontium
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