1,808 research outputs found
Energy and Water Resources Scarcity: Critical Infrastructure for Growth and Economic Development in Arizona and Sonora
Stealthy Deception Attacks Against SCADA Systems
SCADA protocols for Industrial Control Systems (ICS) are vulnerable to
network attacks such as session hijacking. Hence, research focuses on network
anomaly detection based on meta--data (message sizes, timing, command
sequence), or on the state values of the physical process. In this work we
present a class of semantic network-based attacks against SCADA systems that
are undetectable by the above mentioned anomaly detection. After hijacking the
communication channels between the Human Machine Interface (HMI) and
Programmable Logic Controllers (PLCs), our attacks cause the HMI to present a
fake view of the industrial process, deceiving the human operator into taking
manual actions. Our most advanced attack also manipulates the messages
generated by the operator's actions, reversing their semantic meaning while
causing the HMI to present a view that is consistent with the attempted human
actions. The attacks are totaly stealthy because the message sizes and timing,
the command sequences, and the data values of the ICS's state all remain
legitimate.
We implemented and tested several attack scenarios in the test lab of our
local electric company, against a real HMI and real PLCs, separated by a
commercial-grade firewall. We developed a real-time security assessment tool,
that can simultaneously manipulate the communication to multiple PLCs and cause
the HMI to display a coherent system--wide fake view. Our tool is configured
with message-manipulating rules written in an ICS Attack Markup Language (IAML)
we designed, which may be of independent interest. Our semantic attacks all
successfully fooled the operator and brought the system to states of blackout
and possible equipment damage
Impact of total automation consolidating first-line laboratory tests on diagnostic blood loss
Background: Blood loss for laboratory testing may contribute to hospital-acquired anemia. When implementing the core laboratory (core-lab) section, we consolidated first-line tests decreasing the number of tubes previously dispatched to different sites. Here, hypothesized benefits of the amount of blood volume drawn were explored.
Methods: We retrieved, using a laboratory information system (LIS), the number of tubes received by laboratories interested in the change from all clinical wards in a year-based period, i.e. 2013 for pre-core-lab and 2015 for core-lab system, respectively. Data were expressed as the overall number of tubes sent to laboratories, the corresponding blood volume, and the number of laboratory
tests performed, normalized for the number of inpatients.
Results: After consolidation, the average number of blood tubes per inpatient significantly decreased (12.6 vs. 10.7, p\u2009<\u20090.001). However, intensive care units (ICUs) did not reduce the number of tubes per patient, according to the needs of daily monitoring of their clinical status. The average blood volume sent to laboratories did not vary significantly because serum tubes for core-lab required higher volumes for testing up to 55 analytes in the same transaction. Finally, the number of requested tests per
patient during the new osystem slightly decreased ( 122.6%).
Conclusions: Total laboratory automation does not automatically mean reducing iatrogenic blood loss. The new system affected the procedure of blood drawing in clinical wards by significantly reducing the number of handled tubes, producing a benefit in terms of costs, labor and time consumption. Except in ICUs, this also slightly promoted some blood saving. ICUs which engage in phlebotomizing patients daily, did not take advantage from the test consolidation
Primer informe de Aelurostrongylus abstrusus en el caracol de tierra Rumina decollata, en la Ciudad AutĂłnoma de Buenos Aires
Aelurostrongylus abstrusus (Railliet, 1898) is a worldwide distributed lungworm that affects wild and domestic cats, causing bronchopneumonia of varying intensity. Cats became infected by eating slugs and snails with third infective stage larvae (L3). The aim of the study was to describe the presence of A. abstrusus in R. decollate snails. R. decollata specimens and samples of catsâ faeces were collected from the open spaces of a public institution of Buenos Aires city, inhabited by a stray cat population. Catsâ faeces were processed by BaermmanÂŽs technique and snails were digested in pool, by artificial digestion method. First stage larvae of A. abstrusus were recovered from 35.30 % (6/17) of the sampled faeces. An 80 % (20/25) snails pools were positive for the second and third larval stages. Mean value of total larvae recovered per pool was 150.64 and mean value of L3/pool was 93.89. This is the first report of the development of A. abstrusus infective larvae in R. decollate snail as intermediate host, since the relationship between high levels of infection in snails and in catsâ faeces could be demonstrated in catsâ habitat.Aelurostrongylus abstrusus (Railliet, 1898) es un helminto pulmonar mundialmente distribuido que afecta a los gatos, causando bronconeumonias de variada intensidad. La infecciĂłn se produce por ingestiĂłn de babosas y caracoles terrestres con larvas infectantes (L3). El objetivo del estudio fue describir la presencia de A. abstrusus en el caracol R. decollata. Se recolectaron muestras de heces felinas y caracoles presentes en una instituciĂłn pĂșblica de la Ciudad AutĂłnoma de Buenos Aires, habitada por una poblaciĂłn de gatos sin propietario. Las heces fueron procesadas mediante la tĂ©cnica de Baermman y los caracoles fueron digeridos en pool por digestiĂłn artificial enzimĂĄtica. Larvas de primer estadio (L1) de A. abstrusus fueron recuperadas en el 35,30% (6/17) de las heces. El 80% (20/25) de los pooles de caracoles presentĂł larvas de segundo y tercer estadio. El promedio de larvas totales recuperado por pool fue de 150,64 y el valor medio de L3/pool fue de 93.89. Este es el primer hallazgo del desarrollo de larvas infectivas de A. abstrusus en el caracol domĂ©stico R. decollata. Los altos niveles de infecciĂłn encontrados en los caracoles y en las heces de los gatos demuestran el potencial de R. decollata como hospedador intermediario de A. abstrusus.Fil: Cardillo, Natalia Marina. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de ParasitologĂa y Enfermedades Parasitarias; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Clemente, A.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de ParasitologĂa y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, M.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de ParasitologĂa y Enfermedades Parasitarias; ArgentinaFil: BorrĂĄs, P.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de ParasitologĂa y Enfermedades Parasitarias; ArgentinaFil: Rosa, A.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de ParasitologĂa y Enfermedades Parasitarias; ArgentinaFil: Ribicich, M.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de ParasitologĂa y Enfermedades Parasitarias; Argentin
Evaluation of Drug Interactions in Patients Treated with DAAs for Hepatitis C Therapy with Comorbidities and Cardiovascular IssuesâA Delphi Consensus Project
Orally administered direct-acting antivirals (DAAs) have dramatically changed the possibility of curing HCV (hepatitis C virus) infection, with the two principal HCV regimens based on the combination of glecaprevir + pibrentasvir (GLE-PIB) and sofosbuvir + velpatasvir (SOF-VEL). A combination of drugs containing NS3/4A protease inhibitors, as well as the fact that almost all HCV patients can be treated at present, may expose patients to a higher rate of drugâdrug interactions (DDIs). The hepatitis C treatment recommendations from the EASL (European Association for the Study of the Liver) state that, prior to starting treatment with a DAA, a detailed drug history should be taken; yet, the decision on managing the potential DDIs is not always clear. For this reason, a group of Italian cardiologists and hepatologists promoted a survey among colleagues to assess the controversial issues when treating patients with chronic hepatitis C taking concomitant cardiovascular drugs, aiming to reach a consensus on the best practice to apply when treating a patient with chronic hepatitis C who is taking concomitant drugs for cardiovascular diseases. Two consecutive questionnaires were proposed between June and July 2022 to a qualitative Expert Panel (EP) of 14 gastroenterologists, infectologists, hepatologists, and internists, with statistical analyses performed on 100% of the responses for both questionnaires. Agreement among experts was assessed following the Delphi method as developed by the RAND Corporation. The interviewed experts consider DDIs a critical clinical problem to be evaluated in HCV patients. Therefore, dose changes, drug substitution, and discontinuation of concomitant cardiovascular drugs should be discouraged, even if planned for a relatively short period. Since oral DAAs have different DDIs profiles, hepatologists should prefer the antiviral DAA combination presenting the lowest instance of potential interactions
Involvement of the protein ras homolog enriched in the striatum, rhes, in dopaminergic neuronsâ degeneration: Link to parkinsonâs disease
Rhes is one of the most interesting genes regulated by thyroid hormones that, through the inhibition of the striatal cAMP/PKA pathway, acts as a modulator of dopamine neurotransmission. Rhes mRNA is expressed at high levels in the dorsal striatum, with a medialâtoâlateral expression gradient reflecting that of both dopamine D2 and adenosine A2A receptors. Rhes transcript is also present in the hippocampus, cerebral cortex, olfactory tubercle and bulb, substantia nigra pars compacta (SNc) and ventral tegmental area of the rodent brain. In line with Rhesâdependent regulation of dopaminergic transmission, data showed that lack of Rhes enhanced cocaineâ and am-phetamineâinduced motor stimulation in mice. Previous studies showed that pharmacological de-pletion of dopamine significantly reduces Rhes mRNA levels in rodents, nonâhuman primates and Parkinsonâs disease (PD) patients, suggesting a link between dopaminergic innervation and physiological Rhes mRNA expression. Rhes protein binds to and activates striatal mTORC1, and modulates LâDOPAâinduced dyskinesia in PD rodent models. Finally, Rhes is involved in the survival of mouse midbrain dopaminergic neurons of SNc, thus pointing towards a Rhesâdependent modulation of autophagy and mitophagy processes, and encouraging further investigations about mechanisms underlying dysfunctions of the nigrostriatal system
Radiomics-Based Inter-Lesion Relation Network to Describe [18F]FMCH PET/CT Imaging Phenotypes in Prostate Cancer
Advanced image analysis, specifically radiomics, has been recognized as a potential source of biomarkers for cancers. However, there are challenges to its application in the clinic, such as proper description of diseases where multiple lesions coexist. In this study, we aimed to characterize the intra-tumor heterogeneity of metastatic prostate cancer using an innovative approach. This approach consisted of a transformation method to build a radiomic profile of lesions extracted from [18F]FMCH PET/CT images, a qualitative assessment of intra-tumor heterogeneity of patients, and a quantitative representation of the intra-tumor heterogeneity of patients in terms of the relationship between their lesionsâ profiles. We found that metastatic prostate cancer patients had lesions with different radiomic profiles that exhibited intra-tumor radiomic heterogeneity and that the presence of many radiomic profiles within the same patient impacted the outcome
Attacking the V:On the resiliency of adaptive-horizon MPC
Inspired by the emerging problem of CPS security, we introduce the concept of controller-attacker games. A controller-attacker game is a two-player stochastic game, where the two players, a controller and an attacker, have antagonistic objectives. A controller-attacker game is formulated in terms of a Markov Decision Process (MDP), with the controller and the attacker jointly determining the MDPâs transition probabilities. We also introduce the class of controller-attacker games we call V-formation games, where the goal of the controller is to maneuver the plant (a simple model of flocking dynamics) into a V-formation, and the goal of the attacker is to prevent the controller from doing so. Controllers in V-formation games utilize a new formulation of model-predictive control we have developed called Adaptive-Horizon MPC (AMPC), giving them extraordinary power: we prove that under certain controllability conditions, an AMPC controller can attain V-formation with probability 1. We evaluate AMPCâs performance on V-formation games using statistical model checking. Our experiments demonstrate that (a) as we increase the power of the attacker, the AMPC controller adapts by suitably increasing its horizon, and thus demonstrates resiliency to a variety of attacks; and (b) an intelligent attacker can significantly outperform its naive counterpart
Decipher the glioblastoma microenvironment: The first milestone for new groundbreaking therapeutic strategies
Glioblastoma (GBM) is the most common primary malignant brain tumour in adults. Despite the combination of novel therapeutical approaches, it remains a deadly malignancy with an abysmal prognosis. GBM is a polymorphic tumour from both molecular and histological points of view. It consists of different malignant cells and various stromal cells, contributing to tumour initiation, progression, and treatment response. GBMâs microenvironment is multifaceted and is made up of soluble factors, extracellular matrix components, tissue-resident cell types (e.g., neurons, astrocytes, endothelial cells, pericytes, and fibroblasts) together with resident (e.g., microglia) or recruited (e.g., bone marrow-derived macrophages) immune cells. These latter constitute the so-called immune microenvironment, accounting for a substantial GBMâs tumour volume. Despite the abundance of immune cells, an intense state of tumour immunosuppression is promoted and developed; this represents the significant challenge for cancer cellsâ immune-mediated destruction. Though literature data suggest that distinct GBMâs subtypes harbour differences in their microenvironment, its role in treatment response remains obscure. However, an in-depth investigation of GBMâs microenvironment may lead to novel therapeutic opportunities to improve patientsâ outcomes. This review will elucidate the GBMâs microenvironment composition, highlighting the current state of the art in immunotherapy approaches. We will focus on novel strategies of active and passive immunotherapies, including vaccination, gene therapy, checkpoint blockade, and adoptive T-cell therapies
Comparison between in vitro chemical and ex vivo biological assays to evaluate antioxidant capacity of botanical extracts
The anti-oxidative activity of plant-derived extracts is well-known and confers health-promoting effects on functional foods and food supplements. Aim of this work is to evaluate the capability of two different assays to predict the real biological antioxidant efficiency. At this purpose, extracts from five different plant-derived matrices and commercial purified phytochemicals were analyzed for their anti-oxidative properties by using well-standardized in vitro chemical method (TEAC) and an ex vivo biological assay. The biological assay, a cellular membrane system obtained from erythrocytes of healthy volunteers, is based on the capability of phytochemicals treatment to prevent membrane lipid peroxidation under oxidative stress by UV-B radiation. Plant extracts naturally rich in phenols with different structure and purified phytochemicals showed different in vitro and ex vivo antioxidant capacities. A high correlation between phenolic contents of the plant-derived extracts and their ability to prevent oxidative injuries in a biological system was found, thus underlying the relevance of this class of metabolites in preventing oxidative stress. On the other hand, a low correlation between the antioxidant capacities was shown between in vitro and ex vivo antioxidant assay. Moreover, data presented in this work show how food complex matrices are more effective in preventing oxidative damages at biological level than pure phytochemicals, even if for these latter, the antioxidant activity was generally higher than that observed for food complex matrices
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