Sipuncula de la región magallánica comparada con zonas adyacentes de la Antártida

The Magellan sipunculan fauna includes 4 families, 7 genera and 16 species. A comparison between this fauna and that from adjacent regions of Antarctica has been made. A total of 8 species and 3 genera are shared by the compared areas. Univariate analyses show no significant statistical differences among the investigated faunas, whereas a multivariate analysis corroborates that the effects of the Antarctic Convergence are stronger at the level of genera than species. Only 3 genera were able to cross over this zoogeographical boundary. However, these 3 genera succeeded in the new biotopes of Antarctica, since a total of 16 species have been recorded, compared with the 8 species only found in the Magellan region.La fauna magallánica de sipuncúlidos presenta 4 familias, 7 géneros y 16 especies. Se lleva a cabo una comparación entre esta fauna y la procedente de zonas adyacentes de la Antártida. Un total de 8 especies y 3 géneros están presentes en ambas zonas. Los resultados del análisis univariante indican la ausencia de diferencias estadísticas significativas entre las faunas investigadas, mientras que el análisis multivariante revela que los efectos de la Convergencia Antártica son más importantes a nivel de género que de especie. Sin embargo, estos géneros tuvieron cierto éxito en la colonización progresiva de los nuevos biotopos antárticos, dado que se han encontrado un total de 16 especies frente a las 8 exclusivas del área magallánica

Macrozoobenthic changes on rocky shores of the Bight of Bilbao: 14 years of monitoring biological recovery

Benthic communities on rocky shores from the Bight of Bilbao, known as the Abra de Bilbao (northern Spain) and the adjacent coast have been studied during 14 years (1984-1998) within the framework of an environmental monitoring programme for both subtidal and intertidal zones. The study area is now experiencing a process of generalised environmental improvement in its water quality, as a result of the region's industrial recession and the implementation of a comprehensive renovation sewage system, which is still in progress. Biological recovery has been analysed with uni- and multivariate techniques, which make it possible to differentiate several recovery stages, grouped according to the littoral zone sampled. A total of 12 taxa have been selected as characteristics of different environmental situations in space and time, the most sensitive of which are Sagartiidae, Mytilidae, Patellidae and Chthamalidae. The need to use alternative analyses in long term studies of hard bot- tom macrozoobenthos is highlighted. A three-phase conceptual model based on own results and previous works is proposed, which describes the current situation, as well as the recorded or expected changes. The model comprises two parts: a) one for the subtidal and lower intertidal levels, where biotic strategies related to the inverse patterns of fauna and flora dominance are emphasised; b) another for the upper intertidal level, where the appearance and development of a barnacle belt play a crucial role. The model is intended to serve as an environmental tool able to detect changes in the recovery process of the Bight of Bilbao and similar bays elsewhere.Se han estudiado las comunidades bénticas de sustrato rocoso del abra de Bilbao y su costa adyacente durante 14 años (1984-1998), dentro de un programa de seguimiento biológico de los medios submareal e intermareal. El área de estudio se encuentra en un proceso de mejora de la calidad ambiental de sus aguas como consecuencia de la recesión industrial y del Plan Integral de Saneamiento, actualmente en desarrollo. Se ha constatado la recuperación biológica por medio de técnicas univariantes y multivariantes, proponiéndose una serie de etapas en dicha recuperación, diferentes según el nivel litoral. Se ha seleccionado un total de doce taxones como los máximos responsables de discriminar situaciones en el espacio y en el tiempo, entre los que destacan por su mayor sensibilidad: Sagartiidae, Mytilidae, Patellidae y Chthamalidae. Se demuestra, asimismo, la necesidad de utilizar diferentes tipos de análisis en los estudios a largo plazo del macrozoobentos de sustrato rocoso. Con base en estos resultados y en trabajos previos, se articula un modelo conceptual en tres fases, describiendo la situación actual del bentos y los cambios registrados o previsibles. El modelo consta de dos partes: a) el correspondiente a los niveles submareal e intermareal inferior, donde se enfatizan las estrategias relacionadas con los patrones inversos de dominancia de la fauna y la flora; b) el concerniente al nivel intermareal superior, donde desempeñan un papel fundamental la aparición y el desarrollo del cinturón de cirrípedos. De este modo, se pretende dotar a los planes de seguimiento ambiental, tanto del abra de Bilbao como de otras áreas similares, de una herramienta para la detección de cambios en sus planes de recuperación.Instituto Español de Oceanografí

Comparing heat flow models for interpretation of precast quadratic pile heat exchanger thermal response tests

This paper investigates the applicability of currently available analytical, empirical and numerical heat flow models for interpreting thermal response tests (TRT) of quadratic cross section precast pile heat exchangers. A 3D finite element model (FEM) is utilised for interpreting five TRTs by inverse modelling. The calibrated estimates of soil and concrete thermal conductivity are consistent with independent laboratory measurements. Due to the computational cost of inverting the 3D model, simpler models are utilised in additional calibrations. Interpretations based on semi-empirical pile G-functions yield soil thermal conductivity estimates statistically similar to those obtained from the 3D FEM inverse modelling, given minimum testing times of 60 hours. Reliable estimates of pile thermal resistance can only be obtained from type curves computed with 3D FEM models. This study highlights the potential of applying TRTs for sizing quadratic, precast pile heat exchanger foundations

Neuroimagen estructural y funcional en las enfermedades priónicas humanas

INTRODUCTION: Prion diseases are neurodegenerative disorders resulting from the accumulation of a misfolded isoform of the cellular prion protein (PrPc). They can occur as acquired, sporadic or hereditary forms. Although prion diseases show a wide range of phenotypic variations, pathological features and clinical evolution, they are all characterised by a common unfavourable course and a fatal outcome. REVIEW SUMMARY: Some variants, such as kuru, have practically disappeared, while others, for example the variant Creutzfeldt-Jakob (vCJD) or those attributable to iatrogenic causes, are still in force and pose a challenge to current medicine. There are no definitive pre-mortem diagnostic tests, except for vCJD, where a tonsil biopsy detects 100% of the cases. For this reason, diagnostic criteria dependent on statistical probability have had to be created. These require complementary examinations, such as an electroencephalogram (EEG) or the detection of 14-3-3 protein in cerebrospinal fluid (CSF). Only the "pulvinar sign" in magnetic resonance imaging (MRI) has been included as a vCJD diagnostic criterion. The present review discusses neuroimaging findings for each type of prion disease in patients with a definitive histopathological diagnosis. CONCLUSIONS: The aim is to define the usefulness of these complementary examinations as a tool for the diagnosis of this family of neurodegenerative diseases

Theoretical estimates of the anapole magnetizabilities of C4H4X2 cyclic molecules for X=O, S, Se, and Te

Calculations have been carried out for C4H4X2 cyclic molecules, with X=O, S, Se, and Te, characterized by the presence of magnetic-field induced toroidal electron currents and associated orbital anapole moments. The orbital anapole induced by a static nonuniform magnetic field B, with uniform curl C =∇× B, is rationalized via a second-rank anapole magnetizability tensor aαβ , defined as minus the second derivative of the second-order interaction energy with respect to the components Cα and Bβ. The average anapole magnetizability a equals −χ, the pseudoscalar obtained by spatial averaging of the dipole-quadrupole magnetizability χα,βγ . It has different sign for D and L enantiomeric systems and can therefore be used for chiral discrimination. Therefore, in an isotropic chiral medium, a homogeneous magnetic field induces an electronic anapole Aα, having the same magnitude, but opposite sign, for two enantiomorphs.Fil: Pagola, Gabriel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Ferraro, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Provasi, Patricio Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Modelado e Innovación Tecnológica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Modelado e Innovación Tecnologica; ArgentinaFil: Pelloni, Stefano. Universidad de Modena y Reggio Emilia. Departamento de Química; ItaliaFil: Lazzeretti, Paolo. Universidad de Modena y Reggio Emilia. Departamento de Química; Italia; Itali

Broad MICA/B expression in the small bowel mucosa: a link between cellular stress and celiac disease

The MICA/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B+ T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B+ B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in in vitro models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.Fil: Allegretti, Yessica Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bondar, Constanza María. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guzmán, Luciana. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños "Sor María Ludovica" de la Plata; ArgentinaFil: Cueto Rua, Eduardo. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños "Sor María Ludovica" de la Plata; ArgentinaFil: Chopita, Nestor. Provincia de Buenos Aires. Hospital Interzonal General de Agudos Gral. San Martin; ArgentinaFil: Fuertes, Mercedes Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Zwirner, Norberto Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; ArgentinaFil: Chirdo, Fernando Gabriel. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

R1441G but not G2019S mutation enhances LRRK2 mediated Rab10 phosphorylation in human peripheral blood neutrophils

Heterozygous gain-of-kinase function variants in LRRK2 (leucine-rich repeat kinase 2) cause 1–2% of all cases of Parkinson’s disease (PD) albeit with incomplete and age-dependent penetrance. All pathogenic LRRK2 mutations reside within the two catalytic domains of LRRK2—either in its kinase domain (e.g. G2019S) with modest effect or its ROC-COR GTPase domain (e.g. R1441G/H) with large effect on LRRK2 kinase activity. We have previously reported assays to interrogate LRRK2 kinase pathway activity in human bio-samples measuring phosphorylation of its endogenous substrate Rab10, that mirrors LRRK2 kinase activation status. Here, we isolated neutrophils from fresh peripheral blood from 101 participants including 42 LRRK2 mutation carriers (21 with the G2019S and 21 with the R1441G mutations), 27 patients with idiopathic PD, and 32 controls. Using a dual approach, LRRK2 dependent Rab10 phosphorylation at Threonine 73 (pRab10(Thr73)) was measured by quantitative multiplexed immunoblotting for pRab10(Thr73)/total Rab10 as well as targeted mass-spectrometry for absolute pRab10(Thr73) occupancy. We found a significant over fourfold increase in pRab10(Thr73) phosphorylation in carriers of the LRRK2 R1441G mutation irrespective of clinical disease status. The effect of the LRRK2 G2019S mutation did not reach statistical significance. Furthermore, we show that LRRK2 phosphorylation at Serine 935 is not a marker for LRRK2 kinase activity in human neutrophils. When analysing pRab10(Thr73) phosphorylation in post-mortem brain samples, we observed overall high variability irrespective of clinical and LRRK2 mutation status and attributed this mainly to the adverse effect of the peri- and post-mortem period on the stability of posttranslational modifications such as protein phosphorylation. Overall, in vivo LRRK2 dependent pRab10(Thr73) phosphorylation in human peripheral blood neutrophils is a specific, robust and promising biomarker for significant LRRK2 kinase hyperactivation, as with the LRRK2 R1441G mutation. Additional readouts and/or assays may be needed to increase sensitivity to detect modest LRRK2 kinase activation, as with the LRRK2 G2019S mutation. Our assays could be useful for patient stratification and target engagement studies for LRRK2 kinase inhibitors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00401-021-02325-z

Malaria pigment crystals as magnetic micro-rotors: Key for high-sensitivity diagnosis

The need to develop new methods for the high-sensitivity diagnosis of malaria has initiated a global activity in medical and interdisciplinary sciences. Most of the diverse variety of emerging techniques are based on research-grade instruments, sophisticated reagent-based assays or rely on expertise. Here, we suggest an alternative optical methodology with an easy-to- use and cost-effective instrumentation based on unique properties of malaria pigment reported previously and determined quantitatively in the present study. Malaria pigment, also called hemozoin, is an insoluble microcrystalline form of heme. These crystallites show remarkable magnetic and optical anisotropy distinctly from any other components of blood. As a consequence, they can simultaneously act as magnetically driven micro-rotors and spinning polarizers in suspensions. These properties can gain importance not only in malaria diagnosis and therapies, where hemozoin is considered as drug target or immune modulator, but also in the magnetic manipulation of cells and tissues on the microscopic scale