Copepod diversity along the Congo River Basin: a first approach

peer reviewedThe free-living copepods of the Congo River Basin in Africa, the second largest in the world just after the Amazonas River Basin, are still insufficiently known because of problematic accessibility and complex logistics related to sampling. We analyzed samples from 82 sites obtained during expeditions in 2010 and 2013. The Congo River main channel and its main mouth tributaries (1700km; between Kisangani and Kinshasa), and the Kasai River and its main mouth tributaries (600km) were visited. A Schindler-Patallas trap was used in the open waters, with five samplings at each site. Twenty-five taxa were found, with dominance of immature forms (nauplii and copepodites). We recognized three undescribed species of Cyclopoida. The most speciose genera were: Microcyclops (five species), Mesocyclops, , and (three species each). The most frequent species were: Microcyclops varicans (27,6% of the samples) and Cryptocyclops sp. (10,6%). Few presumably cosmopolitan species were found, like pocyclops prasinus, , and Microcyclops varicans. Few diaptomid calanoids were found. Rarefaction and extrapolation curves revealed that the diversity found is about half that estimated considering just the open water species here studied. Spatially, the highest species richness was found in the main channel of the Congo River (23) compared to Kasai (20) and other tributaries (14, 9 from the Congo tributaries, 6 from the Kasai). The abundance of adult individuals was low, with an average of 2,36 individuals per sample when considering all water bodies. A distance-based redundancy analysis based on abundance and Bray Curtis dissimilarity index revealed two large groups of copepod species in the sites of the Congo main channel, plus two minor groups with mixed sites among Congo and Kasai main channels, and tributaries of both rivers, the latter being represented by small groups correlated with nitrogen forms, total phosphorus, and oxygen concentrations. Concerning the two large Congo´s channel groups of species, one was associated with water transparency, chlorophyll, and phytoplankton dominated by Dinophyceae, Chlorophyceae, Cryptophyceae, and diatoms. The other was correlated with conductivity, chlorophyll-a and Cyanophyceae. Kasai main channel sampling sites were dispersed among the four groups resulting from our analysis. Our results indicate that the copepod species in the area are separated into two large groups associated with black and white waters, with low and high primary productivity, respectively. The results showed a high copepod diversity along the Congo Basin as expected for large tropical rivers, and a low abundance for the adult copepods, as in other lotic environments. More species are expected to be discovered in this basin with the continuation of this investigation

Shedding light on zooplankton diversity from the Congo River Basin

peer reviewedThe Congo River Basin is the second largest in the world, and its plankton biota remains completely unknown. We studied the zooplankton diversity across 1700 km of the main channel (from the cities of Kisangani to Kinshasa) and subsequently in the mouths of the 25 largest tributaries during 2013 (N=39), and across 500 km of Kasai-Kwa River and tributaries in 2015 (N=25). We recorded 135 zooplankton species (26 for Testate Amoebae, 56 for Rotifera, 27 for Cladocera and 26 for Copepoda). At least five cladoceran and four copepod species are new. A non-metric multidimensional statistical analysis with Bray Curtis dissimilarity revealed that the zooplankton composition within Congo main channel was more similar than within the mouths of several tributaries and the Kasai-Kwa River basin. In the later, the tributaries were distinct from each other and from the main channel of Kasai River. A distance-based redundancy analysis using Bray-Curtis dissimilarity on abundance data revealed two main groups of species and limnological variables, one comprising sites with high total suspended matter, conductivity, chlorophyll, phytoplanktonabundance (white water rivers), and other with sites with high transparency and dissolved organic carbon concentration (black water rivers). Zooplankton diversity was uniform in the Congo main channel and in the Kasai-Kwa River, with low difference among sites. There was also a distinct third group, unrelated to variables. This study reveals a high diverse zooplankton community in the Congo basin, with new species and distinct community between the studied rivers, but homogeneous along each one

Effects of life phase and schooling patterns on the foraging behaviour of coral-reef fishes from the genus Haemulon

During this study (December 2009 to December 2010), underwater visual surveys using the focal animal method were performed in the coastal reefs of Tamandaré, north-eastern Brazil. The aim was to analyse the effects of the life phase (juvenile and adult) and schooling patterns (school and solitary) on the feeding behaviour (foraging rates and substratum preferences) of four species of the genus Haemulon (Haemulon aurolineatum, Haemulon parra, Haemulon plumieri and Haemulon squamipinna). PERMANOVA analysis (P < 0.05) indicated that ontogenetic changes and schooling patterns directly influence foraging behaviour. Schooling individuals had low foraging rates (mean ± s.d. = 2.3 ± 2.1 bites 10 min−1) and mobility, usually remaining near the bottom; however, solitary fishes had high foraging rates (mean ± s.d. = 12.5 ± 4.6 bites 10 min−1). Juveniles preferred feeding in the water column (75% of the total number of bites), whereas adults foraged mainly in sand (80%) and bare rock (20%). All four Haemulon species displayed similar patterns of feeding behaviour as well as preferences for foraging sites and display competition for food resources. In contrast, little is known about their habitat use and foraging behaviour over the diel cycle, particularly the newly settled and early juvenile stages

Coral reef fish association with macroalgal beds on a tropical reef system in North-eastern Brazil

Macroalgal beds are recognised for their role as nursery and feeding grounds for several marine species. This study was conducted in the Tamandaré reef complex within the limits of the Costa dos Corais Marine Protected Area (MPA), North-eastern Brazil. The macroalgal bed studied is subjected to several disturbances, especially from tourism. The reef fish were assessed with free dives during the day and night using visual censuses within transects (20 × 2 m) and random swims. The percentage algal cover was estimated using quadrats (40 cm2). The behavioural traits of the fish also provided an understanding of their habitat use. In total, 68 fish species were recorded. The most abundant species were representatives of the families Labridae, Pomacentridae, Acanthuridae and Haemulidae. Sargassum polyceratium, Dictyopteris delicatula and Canistrocarpus cervicornis were the most abundant macroalgae. Because the most abundant fish trophic guild included primarily mobile invertebrate feeders (41.8%) and carnivores (28.4%), it is suggested that the high-canopy macroalgae harbour epiphytic invertebrates, which these fish use for food. Thereby, the study area would act as important nursery and feeding grounds. Tourism activity (e.g. laying chairs, trampling and anchoring) may disrupt fish behaviour and life- history traits and result in damage that would require mitigation through environmental awareness and law enforcement in the most affected areas

Yield and botanical composition of a mixed grass-legume pasture in response to maintenance fertilization Produção e composição botânica de uma pastagem consorciada de gramínea e leguminosa em resposta à adubação de manutenção

This study was carried out on a low-productive Brachiaria brizantha cv. Marandu pasture, mixed with Arachis pintoi cv. Belmonte (forage peanut), established 13 years ago in a Red-Yellow Latosol in Acre State, western Brazilian Amazon. The purpose was to evaluate the response of this pasture to different fertilizer combinations, aiming at identifying the nutritional factors responsible for the fall of pasture carrying capacity and for the reduced vigor of forage peanut. It was used a randomized complete block design, with three replications and 12 combinations of nitrogen, phosphorus, potassium, lime, sulfur, and micronutrients (100 kg/ha of N, P2O5 and K2O; 300 kg/ha of dolomitic lime; 30 kg/ha of sulfur and FTE BR-10). Fertilizers were broadcast in 5 × 5-m plots, and the experimental area was isolated from grazing for 35 days. Pasture response to fertilizations showed that nitrogen was the main limiting nutrient to forage production. The other nutrients, applied singly or in a combined way, without nitrogen source, did not change dry matter accumulation rate. Overall, fertilizations with nitrogen provided dry matter accumulation rate of 115 kg/ha/day in comparison to only 32 kg/ha/day when nitrogen was not applied. There was no response from forage peanut to fertilization, so, there are other factors responsible for its reduced vigor of regrowth.<br>Este estudo foi realizado em uma pastagem pouco produtiva de Brachiaria brizantha cv. Marandu, consorciada com Arachis pintoi cv. Belmonte (amendoim-forrageiro), estabelecida há 13 anos em um Latossolo Vermelho-Amarelo no estado do Acre, Amazônia ocidental brasileira. Objetivou-se avaliar a resposta desta pastagem a diferentes combinações de fertilizantes, visando identificar os fatores nutricionais responsáveis pela queda da capacidade de suporte da pastagem e pelo reduzido vigor do amendoim-forrageiro. Foi utilizado o delineamento em blocos completos ao acaso, com três repetições, com 12 combinações de nitrogênio, fósforo, potássio, calcário, enxofre e micronutrientes (100 kg/ha de N, P2O5 e K2O; 300 kg/ha de calcário dolomítico; 30 kg/ha de enxofre e FTE BR-10). Os fertilizantes foram aplicados em cobertura, em parcelas de 5 × 5 m, e a área experimental foi isolada do pastejo durante 35 dias. A resposta do pasto à adubação indicou que o nitrogênio foi o principal nutriente limitante à produção de forragem. Os demais nutrientes, aplicados isoladamente ou de forma combinada, sem uma fonte de nitrogênio, não alteraram a taxa de acúmulo de massa seca. Em média, as adubações com nitrogênio proporcionaram taxa de acúmulo de massa seca de 115 kg/ha/dia, em comparação à de apenas 32 kg/ha/dia quando não foi aplicado nitrogênio. Não houve resposta do amendoim-forrageiro à adubação, logo há outros fatores responsáveis pelo seu reduzido vigor de rebrota