23 research outputs found
45S rDNA external transcribed spacer organization reveals new phylogenetic relationships in Avena genus
Research ArticleThe genus Avena comprises four distinct genomes organized in diploid (AA or CC), tetraploid
(AABB or AACC) and hexaploid species (AACCDD), constituting an interesting model
for phylogenetic analysis. The aim of this work was to characterize 45S rDNA intergenic
spacer (IGS) variability in distinct species representative of Avena genome diversity±A.
strigosa (AA), A. ventricosa (CvCv), A. eriantha (CpCp), A. barbata (AABB), A. murphyi
(AACC), A. sativa (AACCDD) and A. sterilis (AACCDD) through the assessment of the 5'
external transcribed spacer (5'-ETS), a promising IGS region for phylogenetic studies poorly
studied in Avena genus. In this work, IGS length polymorphisms were detected mainly due
to distinct 5'-ETS sequence types resulting from major differences in the number and organization
of repeated motifs. Although species with A genome revealed a 5'-ETS organization
(A-organization) similar to the one previously described in A. sativa, a distinct organization
was unraveled in C genome diploid species (C-organization). Interestingly, such new organization
presents a higher similarity with other Poaceae species than A-genome sequences,
supporting the hypothesis of C-genome being the ancestral Avena genome. Additionally,
polyploid species with both genomes mainly retain the A-genome 5'-ETS organization, confirming
the preferential elimination of C-genome sequences in Avena polyploid species.
Moreover, 5'-ETS sequences phylogenetic analysis consistently clustered the species studied
according to ploidy and genomic constitution supporting the use of ribosomal genes to
highlight Avena species evolutive pathways.info:eu-repo/semantics/publishedVersio
Mapping QTLs for mineral accumulation and shoot dry biomass under different Zn nutritional conditions in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Polyene-lipids: A new tool to image lipids
Microscopy of lipids in living cells is currently hampered by a lack of adequate fluorescent tags. The most frequently used tags, NBD and BODIPY, strongly influence the properties of lipids, yielding analogs with quite different characteristics. Here, we introduce polyene-lipids containing five conjugated double bonds as a new type of lipid tag. Polyene-lipids exhibit a unique structural similarity to natural lipids, which results in minimal effects on the lipid properties. Analyzing membrane phase partitioning, an important biophysical and biological property of lipids, we demonstrated the superiority of polyene-lipids to both NBD- and BODIPY-tagged lipids. Cells readily take up various polyene-lipid precursors and generate the expected end products with no apparent disturbance by the tag. Applying two-photon excitation microscopy, we imaged the distribution of polyene-lipids in living mammalian cells. For the first time, ether lipids, important for the function of the brain, were successfully visualized
Quantitative trait loci analysis for resistance against Turnip mosaic virus based on a doubled-haploid population in Chinese cabbage
Polymorphism analysis in advanced mutant population of oat (Avena sativa L.) using ISSR markers
Fecundity of the autumnal moth depends on pooled geometrid abundance without a time lag: implications for cyclic population dynamics
A wheat disease resistance gene analog of the NBS-LRR class: identification and analysis
Endocytic Trafficking of Sphingomyelin Depends on Its Acyl Chain Length
To study the principles of endocytic lipid trafficking, we introduced pyrene sphingomyelins (PyrSMs) with varying acyl chain lengths and domain partitioning properties into human fibroblasts or HeLa cells. We found that a long-chain, ordered-domain preferring PyrSM was targeted Hrs and Tsg101 dependently to late endosomal compartments and recycled to the plasma membrane in an NPC1- and cholesterol-dependent manner. A short-chain, disordered domain preferring PyrSM recycled more effectively, by using Hrs-, Tsg101- and NPC1-independent routing that was insensitive to cholesterol loading. Similar chain length-dependent recycling was observed for unlabeled sphingomyelins (SMs). The findings 1) establish acyl chain length as an important determinant in the endocytic trafficking of SMs, 2) implicate ESCRT complex proteins and NPC1 in the endocytic recycling of ordered domain lipids to the plasma membrane, and 3) introduce long-chain PyrSM as the first fluorescent lipid tracing this pathway