529 research outputs found
Nuclear currents based on the integral form of the continuity equation
We present an approach to obtain new forms of the nuclear electromagnetic
current, which is based on an integral form of the continuity equation. The
procedure can be used to restore current conservation in model calculations in
which the continuity equation is not verified. Besides, it provides, as a
particular result, the so-called Siegert's form of the nuclear current, first
obtained by Friar and Fallieros by extending Siegert's theorem to arbitrary
values of the momentum transfer. The new currents are explicitly conserved and
permit a straightforward analysis of their behavior at both low and high
momentum transfers. The results are illustrated with a simple nuclear model
which includes a harmonic oscillator mean potential.Comment: 19 pages, revtex, plus 2 PS figure
First measurements of the ^16O(e,e'pn)^14N reaction
This paper reports on the first measurement of the ^16O(e,e'pn)^14N reaction.
Data were measured in kinematics centred on a super-parallel geometry at energy
and momentum transfers of 215 MeV and 316 MeV/c. The experimental resolution
was sufficient to distinguish groups of states in the residual nucleus but not
good enough to separate individual states. The data show a strong dependence on
missing momentum and this dependence appears to be different for two groups of
states in the residual nucleus. Theoretical calculations of the reaction using
the Pavia code do not reproduce the shape or the magnitude of the data.Comment: 10 pages, 11 figures, 2 tables, Accepted for publication in EPJ
Measurement of the LT-asymmetry in \pi^0 electroproduction at the energy of the \Delta (1232) resonance
The reaction p(e,e'p)pi^0 has been studied at Q^2=0.2 (GeV/c)^2 in the region
of W=1232 MeV. From measurements left and right of q, cross section asymmetries
\rho_LT have been obtained in forward kinematics \rho_LT(\theta_\pi^0=20deg) =
(-11.68 +/- 2.36_stat +/- 2.36_sys)$ and backward kinematics
\rho_LT(\theta_\pi^0=160deg) =(12.18 +/- 0.27_stat +/- 0.82_sys). Multipole
ratios \Re(S_1+^* M_1+)/|M_1+|^2 and \Re(S_0+^* M_1+)/|M_1+|^2 were determined
in the framework of the MAID2003 model. The results are in agreement with older
data. The unusally strong negative \Re(S_0+^* M_1+)/|M_1+|^2 required to bring
also the result of Kalleicher et al. in accordance with the rest of the data is
almost excluded.Comment: 7 pages, 7 figures, 4 tables. Changed content. Accepted for
publication in EPJ
The selectivity, voltage-dependence and acid sensitivity of the tandem pore potassium channel TASK-1 : contributions of the pore domains
We have investigated the contribution to ionic
selectivity of residues in the selectivity filter and pore
helices of the P1 and P2 domains in the acid sensitive
potassium channel TASK-1. We used site directed mutagenesis
and electrophysiological studies, assisted by structural
models built through computational methods. We have
measured selectivity in channels expressed in Xenopus
oocytes, using voltage clamp to measure shifts in reversal
potential and current amplitudes when Rb+ or Na+ replaced
extracellular K+. Both P1 and P2 contribute to selectivity,
and most mutations, including mutation of residues in the
triplets GYG and GFG in P1 and P2, made channels nonselective.
We interpret the effects of these—and of other
mutations—in terms of the way the pore is likely to be
stabilised structurally. We show also that residues in the
outer pore mouth contribute to selectivity in TASK-1.
Mutations resulting in loss of selectivity (e.g. I94S, G95A)
were associated with slowing of the response of channels to
depolarisation. More important physiologically, pH sensitivity
is also lost or altered by such mutations. Mutations
that retained selectivity (e.g. I94L, I94V) also retained their
response to acidification. It is likely that responses both to
voltage and pH changes involve gating at the selectivity filter
Faddeev Calculations of Proton-Deuteron Radiative Capture with Exchange Currents
pd capture processes at various energies have been analyzed based on
solutions of 3N-Faddeev equations and using modern NN forces. The application
of the Siegert theorem is compared to the explicit use of - and
-like exchange currents connected to the AV18 NN interaction. Overall
good agreement with cross sections and spin observables has been obtained but
leaving room for improvement in some cases. Feasibility studies for 3NF's
consistently included in the 3N continuum and the 3N bound state have been
performed as well.Comment: Minor changes in notation, ps files for figure
Investigation of the Exclusive ^{3}He(e,e'pn)p Reaction
Cross sections for the ^{3}He(e,e'pn)p reaction were measured for the first
time at energy transfers of 220 and 270 MeV for several momentum transfers
ranging from 300 to 450 MeV/c. Cross sections are presented as a function of
the momentum of the recoil proton and the momentum transfer. Continuum Faddeev
calculations using the Argonne V18 and Bonn-B nucleon-nucleon potentials
overestimate the measured cross sections by a factor 5 at low recoil proton
momentum with the discrepancy becoming much smaller at higher recoil momentum.Comment: 5, pages, 3 figure
Functional and Transcriptional Induction of Aquaporin-1 Gene by Hypoxia; Analysis of Promoter and Role of Hif-1α
Aquaporin-1 (AQP1) is a water channel that is highly expressed in tissues with rapid O2 transport. It has been reported that this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show that hypoxia increases Aqp1 mRNA and protein levels in tissues, namely mouse brain and lung, and in cultured cells, the 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase in the water permeability of 9L cells exposed to hypoxia, supporting the view that hypoxic Aqp1 up-regulation has a functional role. To investigate the molecular mechanisms underlying this regulatory process, transcriptional regulation was studied by transient transfections of mouse endothelial cells with a 1297 bp 5′ proximal Aqp1 promoter-luciferase construct. Incubation in hypoxia produced a dose- and time-dependent induction of luciferase activity that was also obtained after treatments with hypoxia mimetics (DMOG and CoCl2) and by overexpressing stabilized mutated forms of HIF-1α. Single mutations or full deletions of the three putative HIF binding domains present in the Aqp1 promoter partially reduced its responsiveness to hypoxia, and transfection with Hif-1α siRNA decreased the in vitro hypoxia induction of Aqp1 mRNA and protein levels. Our results indicate that HIF-1α participates in the hypoxic induction of AQP1. However, we also demonstrate that the activation of Aqp1 promoter by hypoxia is complex and multifactorial and suggest that besides HIF-1α other transcription factors might contribute to this regulatory process. These data provide a conceptual framework to support future research on the involvement of AQP1 in a range of pathophysiological conditions, including edema, tumor growth, and respiratory diseases
Physiological Properties of Cholinergic and Non-Cholinergic Magnocellular Neurons in Acute Slices from Adult Mouse Nucleus Basalis
The basal forebrain is a series of nuclei that provides cholinergic input to much of the forebrain. The most posterior of these nuclei, nucleus basalis, provides cholinergic drive to neocortex and is involved in arousal and attention. The physiological properties of neurons in anterior basal forebrain nuclei, including medial septum, the diagonal band of Broca and substantia innominata, have been described previously. In contrast the physiological properties of neurons in nucleus basalis, the most posterior nucleus of the basal forebrain, are unknown.Here we investigate the physiological properties of neurons in adult mouse nucleus basalis. We obtained cell-attached and whole-cell recordings from magnocellular neurons in slices from P42-54 mice and compared cholinergic and non-cholinergic neurons, distinguished retrospectively by anti-choline acetyltransferase immunocytochemistry. The majority (70-80%) of cholinergic and non-cholinergic neurons were silent at rest. Spontaneously active cholinergic and non-cholinergic neurons exhibited irregular spiking at 3 Hz and at 0.3 to 13.4 Hz, respectively. Cholinergic neurons had smaller, broader action potentials than non-cholinergic neurons (amplitudes 64+/-3.4 and 75+/-2 mV; half widths 0.52+/-0.04 and 0.33+/-0.02 ms). Cholinergic neurons displayed a more pronounced slow after-hyperpolarization than non-cholinergic neurons (13.3+/-2.2 and 3.6+/-0.5 mV) and were unable to spike at high frequencies during tonic current injection (maximum frequencies of approximately 20 Hz and >120 Hz).Our results indicate that neurons in nucleus basalis share similar physiological properties with neurons in anterior regions of the basal forebrain. Furthermore, cholinergic and non-cholinergic neurons in nucleus basalis can be distinguished by their responses to injected current. To our knowledge, this is the first description of the physiological properties of cholinergic and non-cholinergic neurons in the posterior aspects of the basal forebrain complex and the first study of basal forebrain neurons from the mouse
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