310 research outputs found
Effects of Orthogonal Rotating Electric Fields on Electrospinning Process
Electrospinning is a nanotechnology process whereby an external electric
field is used to accelerate and stretch a charged polymer jet, so as to produce
fibers with nanoscale diameters. In quest of a further reduction in the cross
section of electrified jets hence of a better control on the morphology of the
resulting electrospun fibers, we explore the effects of an external rotating
electric field orthogonal to the jet direction. Through extensive particle
simulations, it is shown that by a proper tuning of the electric field
amplitude and frequency, a reduction of up to a in the aforementioned
radius can be obtained, thereby opening new perspectives in the design of
future ultra-thin electrospun fibres. Applications can be envisaged in the
fields of nanophotonic components as well as for designing new and improved
filtration materials.Comment: 22 pages, 8 figure
One-loop unitarity of scalar field theories on Poincare invariant commutative nonassociative spacetimes
We study scalar field theories on Poincare invariant commutative
nonassociative spacetimes. We compute the one-loop self-energy diagrams in the
ordinary path integral quantization scheme with Feynman's prescription, and
find that the Cutkosky rule is satisfied. This property is in contrast with
that of noncommutative field theory, since it is known that noncommutative
field theory with space/time noncommutativity violates unitarity in the above
standard scheme, and the quantization procedure will necessarily become
complicated to obtain a sensible Poincare invariant noncommutative field
theory. We point out a peculiar feature of the non-locality in our
nonassociative field theories, which may explain the property of the unitarity
distinct from noncommutative field theories. Thus commutative nonassociative
field theories seem to contain physically interesting field theories on
deformed spacetimes.Comment: 25 pages, 9 figures ; appendix and references adde
Polarization-Resolved Extreme Ultraviolet Second Harmonic Generation from LiNbO
Second harmonic generation (SHG) spectroscopy ubiquitously enables the
investigation of surface chemistry, interfacial chemistry as well as symmetry
properties in solids. Polarization-resolved SHG spectroscopy in the visible to
infrared regime is regularly used to investigate electronic and magnetic orders
through their angular anisotropies within the crystal structure. However, the
increasing complexity of novel materials and emerging phenomena hamper the
interpretation of experiments solely based on the investigation of hybridized
valence states. Here, polarization-resolved SHG in the extreme ultraviolet
(XUV-SHG) is demonstrated for the first time, enabling element-resolved angular
anisotropy investigations. In non-centrosymmetric LiNbO, elemental
contributions by lithium and niobium are clearly distinguished by energy
dependent XUV-SHG measurements. This element-resolved and symmetry-sensitive
experiment suggests that the displacement of Li ions in LiNbO, which is
known to lead to ferroelectricity, is accompanied by distortions to the Nb ion
environment that breaks the inversion symmetry of the NbO octahedron as
well. Our simulations show that the measured second harmonic spectrum is
consistent with Li ion displacements from the centrosymmetric position by
0.5 Angstrom while the Nb-O bonds are elongated/contracted by
displacements of the O atoms by 0.1 Angstrom. In addition, the
polarization-resolved measurement of XUV-SHG shows excellent agreement with
numerical predictions based on dipole-induced SHG commonly used in the optical
wavelengths. This constitutes the first verification of the dipole-based SHG
model in the XUV regime. The findings of this work pave the way for future
angle and time-resolved XUV-SHG studies with elemental specificity in condensed
matter systems
Autosomal dominant pseudohypoaldosteronism type 1 with a novel splice site mutation in MR gene
<p>Abstract</p> <p>Background</p> <p>Autosomal dominant pseudohypoaldosteronism type 1 (PHA1) is a rare inherited condition that is characterized by renal resistance to aldosterone as well as salt wasting, hyperkalemia, and metabolic acidosis. Renal PHA1 is caused by mutations of the human mineralcorticoid receptor gene (<it>MR</it>), but it is a matter of debate whether <it>MR </it>mutations cause mineralcorticoid resistance via haploinsufficiency or dominant negative mechanism. It was previously reported that in a case with nonsense mutation the mutant mRNA was absent in lymphocytes because of nonsense mediated mRNA decay (NMD) and therefore postulated that haploinsufficiency alone can give rise to the PHA1 phenotype in patients with truncated mutations.</p> <p>Methods and Results</p> <p>We conducted genomic DNA analysis and mRNA analysis for familial PHA1 patients extracted from lymphocytes and urinary sediments and could detect one novel splice site mutation which leads to exon skipping and frame shift result in premature termination at the transcript level. The mRNA analysis showed evidence of wild type and exon-skipped RT-PCR products.</p> <p>Conclusion</p> <p>mRNA analysis have been rarely conducted for PHA1 because kidney tissues are unavailable for this disease. However, we conducted RT-PCR analysis using mRNA extracted from urinary sediments. We could demonstrate that NMD does not fully function in kidney cells and that haploinsufficiency due to NMD with premature termination is not sufficient to give rise to the PHA1 phenotype at least in this mutation of our patient. Additional studies including mRNA analysis will be needed to identify the exact mechanism of the phenotype of PHA.</p
The association of long-term outcome and biological sex in patients with acute heart failure from different geographic regions
Aims: Recent data from national registries suggest that acute heart failure (AHF) outcomes might vary in men and women, however, it is not known whether this observation is universal. The aim of this study was to evaluate the association of biological sex and 1-year all-cause mortality in patients with AHF in various regions of the world. Methods and results: We analysed several AHF cohorts including GREAT registry (22 523 patients, mostly from Europe and Asia) and OPTIMIZE-HF (26 376 patients from the USA). Clinical characteristics and medication use at discharge were collected. Hazard ratios (HRs) for 1-year mortality according to biological sex were calculated using a Cox proportional hazards regression model with adjustment for baseline characteristics (e.g. age, comorbidities, clinical and laboratory parameters at admission, left ventricular ejection fraction). In the GREAT registry, women had a lower risk of death in the year following AHF [HR 0.86 (0.79-0.94), P < 0.001 after adjustment]. This was mostly driven by northeast Asia [n = 9135, HR 0.76 (0.67-0.87), P < 0.001], while no significant differences were seen in other countries. In the OPTIMIZE-HF registry, women also had a lower risk of 1-year death [HR 0.93 (0.89-0.97), P < 0.001]. In the GREAT registry, women were less often prescribed with a combination of angiotensin-converting enzyme inhibitors and beta-blockers at discharge (50% vs. 57%, P = 0.001). Conclusion: Globally women with AHF have a lower 1-year mortality and less evidenced-based treatment than men. Differences among countries need further investigation. Our findings merit consideration when designing future global clinical trials in AHF
TLR7 single-nucleotide polymorphisms in the 3' untranslated region and intron 2 independently contribute to systemic lupus erythematosus in Japanese women: a case-control association study
IntroductionThe Toll-like receptor 7 (TLR7) gene, encoded on human chromosome Xp22.3, is crucial for type I interferon production. A recent multicenter study in East Asian populations, comprising Chinese, Korean and Japanese participants, identified an association of a TLR7 single-nucleotide polymorphism (SNP) located in the 3\u27 untranslated region (3\u27 UTR), rs3853839, with systemic lupus erythematosus (SLE), especially in males, although some difference was observed among the tested populations. To test whether additional polymorphisms contribute to SLE in Japanese, we systematically analyzed the association of TLR7 with SLE in a Japanese female population.MethodsA case-control association study was conducted on eight tag SNPs in the TLR7 region, including rs3853839, in 344 Japanese females with SLE and 274 healthy female controls.ResultsIn addition to rs3853839, two SNPs in intron 2, rs179019 and rs179010, which were in moderate linkage disequilibrium with each other (r2 = 0.53), showed an association with SLE (rs179019: P = 0.016, odds ratio (OR) 2.02, 95% confidence interval (95% CI) 1.15 to 3.54; rs179010: P = 0.018, OR 1.75, 95% CI 1.10 to 2.80 (both under the recessive model)). Conditional logistic regression analysis revealed that the association of the intronic SNPs and the 3\u27 UTR SNP remained significant after we adjusted them for each other. When only the patients and controls carrying the risk genotypes at the 3\u27 UTR SNPpositionwere analyzed, the risk of SLE was significantly increased when the individuals also carried the risk genotypes at both of the intronic SNPs (P = 0.0043, OR 2.45, 95% CI 1.31 to 4.60). Furthermore, the haplotype containing the intronic risk alleles in addition to the 3\u27 UTR risk allele was associated with SLE under the recessive model (P = 0.016, OR 2.37, 95% CI 1.17 to 4.80), but other haplotypes were not associated with SLE.ConclusionsThe TLR7 intronic SNPs rs179019 and rs179010 are associated with SLE independently of the 3\u27 UTR SNP rs3853839 in Japanese women. Our findings support a role of TLR7 in predisposition for SLE in Asian populations
Genetic Variation in the Proximal Promoter of ABC and SLC Superfamilies: Liver and Kidney Specific Expression and Promoter Activity Predict Variation
Membrane transporters play crucial roles in the cellular uptake and efflux of an array of small molecules including nutrients, environmental toxins, and many clinically used drugs. We hypothesized that common genetic variation in the proximal promoter regions of transporter genes contribute to observed variation in drug response. A total of 579 polymorphisms were identified in the proximal promoters (−250 to +50 bp) and flanking 5′ sequence of 107 transporters in the ATP Binding Cassette (ABC) and Solute Carrier (SLC) superfamilies in 272 DNA samples from ethnically diverse populations. Many transporter promoters contained multiple common polymorphisms. Using a sliding window analysis, we observed that, on average, nucleotide diversity (π) was lowest at approximately 300 bp upstream of the transcription start site, suggesting that this region may harbor important functional elements. The proximal promoters of transporters that were highly expressed in the liver had greater nucleotide diversity than those that were highly expressed in the kidney consistent with greater negative selective pressure on the promoters of kidney transporters. Twenty-one promoters were evaluated for activity using reporter assays. Greater nucleotide diversity was observed in promoters with strong activity compared to promoters with weak activity, suggesting that weak promoters are under more negative selective pressure than promoters with high activity. Collectively, these results suggest that the proximal promoter region of membrane transporters is rich in variation and that variants in these regions may play a role in interindividual variation in drug disposition and response
The discovery and follow-up of four transiting short-period sub-Neptunes orbiting M dwarfs
Sub-Neptunes with radii of 2–3 R⊕ are intermediate in size between rocky planets and Neptune-sized planets. The orbital properties and bulk compositions of transiting sub-Neptunes provide clues to the formation and evolution of close-in small planets. In this paper, we present the discovery and follow-up of four sub-Neptunes orbiting M dwarfs (TOI-782, TOI-1448, TOI-2120, and TOI-2406), three of which were newly validated by ground-based follow-up observations and statistical analyses. TOI-782 b, TOI-1448 b, TOI-2120 b, and TOI-2406 b have radii of Rp = 2.740 +0.082-0.079 R⊕, 2.769+0.073-0.068 R⊕, 2.120 ± 0.067 R⊕, and 2.830+0.068-0.066 R⊕ and orbital periods of P = 8.02, 8.11, 5.80, and 3.08 days, respectively. Doppler monitoring with the Subaru/InfraRed Doppler instrument led to 2σ upper limits on the masses of <19.1 M⊕, <19.5 M⊕, <6.8 M⊕, and <15.6 M⊕ for TOI-782 b, TOI-1448 b, TOI-2120 b, and TOI-2406 b, respectively. The mass–radius relationship of these four sub-Neptunes testifies to the existence of volatile material in their interiors. These four sub-Neptunes, which are located above the so-called "radius valley," are likely to retain a significant atmosphere and/or an icy mantle on the core, such as a water world. We find that at least three of the four sub-Neptunes (TOI-782 b, TOI-2120 b, and TOI-2406 b), orbiting M dwarfs older than 1 Gyr, are likely to have eccentricities of e ∼ 0.2–0.3. The fact that tidal circularization of their orbits is not achieved over 1 Gyr suggests inefficient tidal dissipation in their interiors.Peer reviewe
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