Connexins and pannexins in the skeleton: gap junctions, hemichannels and more

Regulation of bone homeostasis depends on the concerted actions of bone-forming osteoblasts and bone-resorbing osteoclasts, controlled by osteocytes, cells derived from osteoblasts surrounded by bone matrix. The control of differentiation, viability and function of bone cells relies on the presence of connexins. Connexin43 regulates the expression of genes required for osteoblast and osteoclast differentiation directly or by changing the levels of osteocytic genes, and connexin45 may oppose connexin43 actions in osteoblastic cells. Connexin37 is required for osteoclast differentiation and its deletion results in increased bone mass. Less is known on the role of connexins in cartilage, ligaments and tendons. Connexin43, connexin45, connexin32, connexin46 and connexin29 are expressed in chondrocytes, while connexin43 and connexin32 are expressed in ligaments and tendons. Similarly, although the expression of pannexin1, pannexin2 and pannexin3 has been demonstrated in bone and cartilage cells, their function in these tissues is not fully understood

X-ray and Multiwavelength Insights into the Nature of Weak Emission-Line Quasars at Low Redshift

(Abridged) We report on the X-ray and multiwavelength properties of 11 radio-quiet quasars with weak or no emission lines identified by the Sloan Digital Sky Survey (SDSS) with redshift z=0.4-2.5. The distribution of relative X-ray brightness for our low-redshift weak-line quasar (WLQ) candidates is significantly different from that of typical radio-quiet quasars, having an excess of X-ray weak sources, but it is consistent with that of high-redshift WLQs. The X-ray weak sources generally show similar UV emission-line properties to those of the X-ray weak quasar PHL 1811; they may belong to the notable class of PHL 1811 analogs. The average X-ray spectrum of these sources is somewhat harder than that of typical radio-quiet quasars. Several other low-redshift WLQ candidates have normal ratios of X-ray-to-optical/UV flux, and their average X-ray spectral properties are also similar to those of typical radio-quiet quasars. The X-ray weak and X-ray normal WLQ candidates may belong to the same subset of quasars having high-ionization "shielding gas" covering most of the wind-dominated broad emission-line region, but be viewed at different inclinations. The mid-infrared-to-X-ray spectral energy distributions (SEDs) of these sources are generally consistent with those of typical SDSS quasars, showing that they are not likely to be BL Lac objects with relativistically boosted continua and diluted emission lines. However, one source in our X-ray observed sample is remarkably strong in X-rays, indicating that a small fraction of low-redshift WLQ candidates may actually be BL Lacs residing in the radio-faint tail of the BL Lac population. We also investigate universal selection criteria for WLQs over a wide range of redshift, finding that it is not possible to select WLQ candidates in a fully consistent way using different prominent emission lines as a function of redshift.Comment: ApJ in press; 26 pages, 11 figures and 7 tables. The full Table 3 is available upon reques

Heteropolymer Sequence Design and Preferential Solvation of Hydrophilic Monomers: One More Application of Random Energy Model

In this paper, we study the role of surface of the globule and the role of interactions with the solvent for designed sequence heteropolymers using random energy model (REM). We investigate the ground state energy and surface monomer composition distribution. By comparing the freezing transition in random and designed sequence heteropolymers, we discuss the effects of design. Based on our results, we are able to show under which conditions solvation effect improves the quality of sequence design. Finally, we study sequence space entropy and discuss the number of available sequences as a function of imposed requirements for the design quality

The Ultraviolet-to-Mid-Infrared Spectral Energy Distribution of Weak Emission Line Quasars

We present Spitzer Space Telescope photometry of 18 Sloan Digital Sky Survey (SDSS) quasars at 2.7 <= z <= 5.9 which have weak or undetectable high-ionization emission lines in their rest-frame ultraviolet (UV) spectra (hereafter weak-lined quasars, or WLQs). The Spitzer data are combined with SDSS spectra and ground-based, near-infrared (IR) photometry of these sources to produce a large inventory of spectral energy distributions (SEDs) of WLQs across the rest-frame ~0.1-5 mum spectral band. The SEDs of our sources are inconsistent with those of BL Lacertae objects which are dominated by synchrotron emission due to a jet aligned close to our line-of-sight, but are consistent with the SED of ordinary quasars with similar luminosities and redshifts that exhibit a near-to-mid-IR 'bump', characteristic of hot dust emission. This indicates that broad emission lines in WLQs are intrinsically weak, rather than suffering continuum dilution from a jet, and that such sources cannot be selected efficiently from traditional photometric surveys.Comment: 10 pages (emulateapj), 4 figures. Accepted for publication in Ap

Preventing Successor Liability for Defective Products: Safeguards for Acquiring Corporations

In recent years, many courts have determined longstanding limitations on successor liability to be insufficiently sensitive to the compensation needs of products liability claimants. In response, courts in several jurisdictions have eroded traditional corporate law immunities to successor liability for defective products. Although this liberalization of the principles of successor liability has expanded the range of potential defendants in many tort lawsuits, the expansion of liability has caused an increase in uncertainty in corporate transactions, and an increase in complex legal and logistical maneuvering to avoid the growing liability web. This Article seeks to outline the expansion of successor liability in the products area, note the negative implications of that expansion, and identify mechanisms to prevent the attachment of successor liability under both the traditional and expanded regimes. The Article also prescribes a statutory alternative to create much needed stability and certainty in this area through a straightforward allocation of liabilities between purchasers and sellers

Sound and complete axiomatizations of coalgebraic language equivalence

Coalgebras provide a uniform framework to study dynamical systems, including several types of automata. In this paper, we make use of the coalgebraic view on systems to investigate, in a uniform way, under which conditions calculi that are sound and complete with respect to behavioral equivalence can be extended to a coarser coalgebraic language equivalence, which arises from a generalised powerset construction that determinises coalgebras. We show that soundness and completeness are established by proving that expressions modulo axioms of a calculus form the rational fixpoint of the given type functor. Our main result is that the rational fixpoint of the functor $FT$, where $T$ is a monad describing the branching of the systems (e.g. non-determinism, weights, probability etc.), has as a quotient the rational fixpoint of the "determinised" type functor $\bar F$, a lifting of $F$ to the category of $T$-algebras. We apply our framework to the concrete example of weighted automata, for which we present a new sound and complete calculus for weighted language equivalence. As a special case, we obtain non-deterministic automata, where we recover Rabinovich's sound and complete calculus for language equivalence.Comment: Corrected version of published journal articl

A systematically coarse-grained model for DNA, and its predictions for persistence length, stacking, twist, and chirality

We introduce a coarse-grained model of DNA with bases modeled as rigid-body ellipsoids to capture their anisotropic stereochemistry. Interaction potentials are all physicochemical and generated from all-atom simulation/parameterization with minimal phenomenology. Persistence length, degree of stacking, and twist are studied by molecular dynamics simulation as functions of temperature, salt concentration, sequence, interaction potential strength, and local position along the chain, for both single- and double-stranded DNA where appropriate. The model of DNA shows several phase transitions and crossover regimes in addition to dehybridization, including unstacking, untwisting, and collapse which affect mechanical properties such as rigidity and persistence length. The model also exhibits chirality with a stable right-handed and metastable left-handed helix.Comment: 30 pages, 20 figures, Supplementary Material available at http://www.physics.ubc.ca/~steve/publications.htm