Characterisation of biodiversity in improved rubber agroforests in West-Kalimantan, Indonesia. Real and Potential uses for spontaneous plants

Since the introduction of rubber at the turn of the 20th century smallholders have developed an original complex agroforestry system called jungle rubber in which non selected young rubber trees (seedlings) are managed extensively alongside secondary forest re-growth. The issue of improving smallholder rubber productivity at affordable capital investments and levels of inputs while maintaining the environmental benefits of jungle rubber has been addressed by the Smallholder Rubber Agroforestry Project (SRAP: a joint project run by ICRAF, GAPKINDO and CIRAD). In 1995-1996, 27 trials (with a total of 100 plots) were set up in three provinces in Indonesia to assess the possibility of associating clonal rubber with agroforestry practices under smallholder conditions (Penot, 1997). Two RAS types were selected for this study: RAS n° 1 and n° 3. RAS n° 1 is basically improved jungle rubber using clonal planting material (see a description of RAS types in annexe 1). The rubber trees are in competition with spontaneous vegetation in the inter-row but results show that there are no negative consequences for rubber growth during the immature period. RAS n° 3 was designed for areas infested by Imperata cylindrica, with the establishment of shrubby leguminous cover crops and fast-growing tree species in the inter-rows with the aim of shading out weeds. The other type, RAS n° 2, is based on intercropping clonal rubber with various annual and perennial crops, including fruit and timber trees (Penot et al, 1994). In all cases, RAS have a planting density of 550 clonal rubber trees/ha and a variable number of associated fruit, timber or fast growing shade trees (from 92 to 256/ha). In addition to the RAS experimental plots, “RAS sendiri” (or “endogenous RAS”) are rubber agroforests improved by farmers without outside assistance. The district of Sanggau in the province of West Kalimantan was identified by SRAP as representative of traditional jungle-rubber-based local farming systems that have developed over the last 90 years. The district of Sanggau is located in the central area of the Kapuas river basin, between 1° N and 0°6' S and 09°8' W and 11°33' E. The district covers 18 302 km2, i.e. 13 % of the province. The trial plots described in this study are located in the villages of Embaong, Engkayu, Kopar, and Trimulia (the last being in the transmigration area). Most soils in the province of West-Kalimantan are acrisoils associated with ferralitic soils. Such soils have relatively good physical characteristics but poor chemical value and become acid. Rubber is widely grown in this area as it can grow in poor soils. The landscape is dominated by logged-over forest, secondary forest and a mosaic of jungle rubber and fallow with secondary forest re-growth. Large scale logging activities took place from 1950s to the 1980s at the expense of primary forest. At present, forested areas are located in hilly or remote areas and are very limited in extent. Oil palm and Acacia mangium plantations developed exponentially in the 1990s increasing the conversion of degraded forest areas into Estates that cultivate perennial crops. The main objective of this study is to assess existing plant biodiversity in RAS systems compared to that of jungle rubber. The second objective is to review the current uses of certain plants and their market potential

Field trip to West Kalimantan. Mission report. Identification of smallholder rubber agroforestry systems : sustainable alternatives

L'objectif principal de ce document, outre le rapport des activités, est de présenter la méthodologie du SRAP et les priorités identifiées par l'équipe (après analyse in situ de la situation locale) en terme de recherche, expérimentation à la plantation, enquêtes à réaliser. Visite du projet PKR-GK (West-Kalimatan smallholder rubber development project) du GAPKINDO. Identification des partenaires pour la réalisation de la phase 1 (1994-1995) du projet SRAP. Quatre types d'essais en champs ont été identifiés et sont décrits en annex

Neutrophil CD64 as a marker of infection in patients admitted to the emergency department with acute respiratory failure

Introduction: Cluster of differentiation 64 (CD64) is expressed on neutrophils during bacterial infections and sepsis. The aim of our study was to assess the CD64 expression in patients admitted to the emergency department (ED) with a triage diagnosis of acute respiratory failure (ARF) and/or dyspnea and to verify a relationship between its value and the presence of infection. Methods: We assessed neutrophil CD64 expression in peripheral blood of patients admitted to the ED with a diagnosis of ARF and/or dyspnea from September 2012 to April 2013. We mea- sured CD64 index by flow cytometry (Leuko64TM kit) and classified patients as infected within 12 hours from admission, without an infection within 12 hours but infected within 72 hours from admission, and not infected. The primary outcome was differentiating CD64 values of patients with a diagnosis of infection within 12 hours and 72 hours from admission, from those of patients without a diagnosis of infection. The secondary outcome was verifying a relationship between CD64 values and patients’ characteristics, Sequential Organ Failure Assessment score, and intensive care unit admission. Results: Of 212 patients included in the study, 40.1% were classified as infected within 12 hours from admission, 20.3% were without an infection 12 hours after admission but were infected within 72 hours, and 39.6% were not infected. The median CD64 index was higher in patients with an infection on admission (CD64 index: 3.58) than in those not considered infected (CD64 index: 1.37), P,0.0001. Among patients not infected at admission, the CD64 index was higher in those with an infection detected during the following hours of observation (CD64 index: 2.75) than in patients without a diagnosis of infection (CD64 index: 1.28), P,0.0001. A CD64 index .3.65 showed a sensitivity of 94.6%, a specificity of 86.8%, and an area under the receiver-operating characteristic curve of 0.952 for prediction of intensive care unit admission. Conclusion: CD64 index could represent a useful diagnostic tool for differential diagnosis of ARF in the ED

Testicular fine needle aspiration as a diagnostic tool in non-obstructive azoospermia

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Mechanism of Human Papillomavirus Binding to Human Spermatozoa and Fertilizing Ability of Infected Spermatozoa

Human papillomaviruses (HPVs) are agents of the most common sexually transmitted diseases in females and males. Precise data about the presence, mechanism of infection and clinical significance of HPV in the male reproductive tract and especially in sperm are not available. Here we show that HPV can infect human sperm, it localizes at the equatorial region of sperm head through interaction between the HPV capsid protein L1 and syndecan-1. Sperm transfected with HPV E6/E7 genes and sperm exposed to HPV L1 capsid protein are capable to penetrate the oocyte and transfer the virus into oocytes, in which viral genes are then activated and transcribed. These data show that sperm might function as vectors for HPV transfer into the oocytes, and open new perspectives on the role of HPV infection in males and are particularly intriguing in relation to assisted reproduction techniques

Increased Cardiovascular Risk Associated with Chemical Sensitivity to Perfluoro-Octanoic Acid: Role of Impaired Platelet Aggregation

Perfluoro\u2013alkyl substances (PFAS), particularly perfluoro\u2013octanoic acid (PFOA), are persisting environmental chemicals showing bioaccumulation in human tissues. Recently, exposure to PFAS has been associated with increased prevalence of cardiovascular diseases (CVDs). However, a causal role of PFAS in atherosclerosis pathogenesis is under-investigated. Here, we investigated the effect of PFOA exposure on platelets\u2019 function, a key player in atherosclerosis process. PFOA accumulation in platelets was evaluated by liquid chromatography-mass spectrometry. Changes in platelets\u2019 membrane fluidity and activation after dose-dependent exposure to PFOA were evaluated by merocyanine 540 (MC540) and anti P-Selectin immune staining at flow cytometry, respectively. Intracellular calcium trafficking was analyzed with Fluo4M probe, time-lapse live imaging. Platelets\u2019 aggregation state was also evaluated with Multiplate\uae aggregometry analyzer in 48 male subjects living in a specific area of the Veneto region with high PFAS environmental pollution, and compared with 30 low-exposure control subjects. Platelets\u2019 membrane was the major target of PFOA, whose dose-dependent accumulation was associated in turn with increased membrane fluidity, as expected by a computational model; increased activation at resting condition; and both calcium uptake and aggregation upon activation. Finally, exposed subjects had higher serum and platelets levels of PFOA, together with increased aggregation parameters at Multiplate\uae, compared with controls. These data help to explain the emerging association between PFAS exposure and CVD

Is Adnexectomy Mandatory at the Time of Hysterectomy for Uterine Sarcomas? A Systematic Review and Meta-Analysis.

Background and Objectives: Uterine sarcomas represents only 3% of all the female genital tract ones. The tumoral stage is the most significant prognostic factor. The role of the bilateral salpingo-oophorectomy (BSO) in the surgical management of FIGO stage IA and IB appears still controversial. This review aims to investigate the impact of bilateral adnexectomy in the treatment of uterine sarcoma. Methods: Following the recommendations in the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, we systematically searched the PubMed, Scopus, Cochrane, Medline, and Medscape databases in February 2022. We applied no language or geographical restrictions, but we considered only English studies. We included the studies containing data about Recurrence Rate (RR), Disease-free Survival (DFS), and Overall Survival (OS). We used comparative studies for meta-analysis. Results: Seventeen studies fulfilled the inclusion criteria; 2 retrospective observational studies, and 15 retrospective comparative studies, And 14 out of the 15 comparative studies were enrolled in meta-analysis. A total of 3743 patients were analyzed concerning the use of adnexectomy with hysterectomy in patients with uterine sarcoma and compared with those who did not. Meta-analysis highlighted a non-significant worsening of the OS in the BSO group compared to the OP group and showed that adnexectomy does not improve the DFS (BSO OR 1.23 (95% CI 0.81–1.85) p = 0.34; I(2) = 24% p = 0.22). Conclusions: Most studies selected for our review showed that adnexectomy does not significantly affect the RR, OS, and PFS in treating FIGO stage I uterine sarcomas. Therefore, even if there is a unanimous consensus about bilateral adnexectomy in menopausal patients, preservation of ovarian tissue may be considered in premenopausal women. Nonetheless, there are not enough cases in the literature to recommend this procedure