On the Complexity of Local Distributed Graph Problems

This paper is centered on the complexity of graph problems in the well-studied LOCAL model of distributed computing, introduced by Linial [FOCS '87]. It is widely known that for many of the classic distributed graph problems (including maximal independent set (MIS) and $(\Delta+1)$-vertex coloring), the randomized complexity is at most polylogarithmic in the size $n$ of the network, while the best deterministic complexity is typically $2^{O(\sqrt{\log n})}$. Understanding and narrowing down this exponential gap is considered to be one of the central long-standing open questions in the area of distributed graph algorithms. We investigate the problem by introducing a complexity-theoretic framework that allows us to shed some light on the role of randomness in the LOCAL model. We define the SLOCAL model as a sequential version of the LOCAL model. Our framework allows us to prove completeness results with respect to the class of problems which can be solved efficiently in the SLOCAL model, implying that if any of the complete problems can be solved deterministically in $\log^{O(1)} n$ rounds in the LOCAL model, we can deterministically solve all efficient SLOCAL-problems (including MIS and $(\Delta+1)$-coloring) in $\log^{O(1)} n$ rounds in the LOCAL model. We show that a rather rudimentary looking graph coloring problem is complete in the above sense: Color the nodes of a graph with colors red and blue such that each node of sufficiently large polylogarithmic degree has at least one neighbor of each color. The problem admits a trivial zero-round randomized solution. The result can be viewed as showing that the only obstacle to getting efficient determinstic algorithms in the LOCAL model is an efficient algorithm to approximately round fractional values into integer values

Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation.

We have previously shown that the C-type Natriuretic Peptide (CNP), a peptide produced by lungs, is able to impact Pseudomonasaeruginosa physiology. In the present work, the effect of CNP at different concentrations on P. aeruginosa biofilm formation was studied and the mechanisms of action of this human hormone on P. aeruginosa were deciphered. CNP was shown to inhibit dynamic biofilm formation in a dose-dependent manner without affecting the bacterial growth at any tested concentrations. The most effective concentrations were 1 and 0.1 µM. At 0.1 µM, the biofilm formation inhibition was fully dependent on the CNP sensor protein AmiC, whereas it was only partially AmiC-dependent at 1 µM, revealing the existence of a second AmiC-independent mode of action of CNP on P. aeruginosa. At 1 µM, CNP reduced both P. aeruginosa adhesion on glass and di-rhamnolipid production and also increased the bacterial membrane fluidity. The various effects of CNP at 1 µM and 0.1 µM on P. aeruginosa shown here should have major consequences to design drugs for biofilm treatment or prevention

Extracellular DNA release, quorum sensing, and PrrF1/F2 small RNAs are key players in Pseudomonas aeruginosa tobramycin-enhanced biofilm formation

Biofilms are structured microbial communities that are the leading cause of numerous chronic infections which are difficult to eradicate. Within the lungs of individuals with cystic fibrosis (CF), Pseudomonas aeruginosa causes persistent biofilm infection that is commonly treated with aminoglycoside antibiotics such as tobramycin. However, sublethal concentrations of this aminoglycoside were previously shown to increase biofilm formation by P. aeruginosa, but the underlying adaptive mechanisms still remain elusive. Herein, we combined confocal laser scanning microscope analyses, proteomics profiling, gene expression assays and phenotypic studies to unravel P. aeruginosa potential adaptive mechanisms in response to tobramycin exposure during biofilm growth. Under this condition, we show that the modified biofilm architecture is related at least in part to increased extracellular DNA (eDNA) release, most likely as a result of biofilm cell death. Furthermore, the activity of quorum sensing (QS) systems was increased, leading to higher production of QS signaling molecules. We also demonstrate upon tobramycin exposure an increase in expression of the PrrF small regulatory RNAs, as well as expression of iron uptake systems. Remarkably, biofilm biovolumes and eDNA relative abundances in pqs and prrF mutant strains decrease in the presence of tobramycin. Overall, our findings offer experimental evidences for a potential adaptive mechanism linking PrrF sRNAs, QS signaling, biofilm cell death, eDNA release, and tobramycin-enhanced biofilm formation in P. aeruginosa. These specific adaptive mechanisms should be considered to improve treatment strategies against P. aeruginosa biofilm establishment in CF patients’ lungs

Activation of the cell wall stress response in pseudomonas aeruginosa infected by a pf4 phage variant

Pseudomonas aeruginosa PAO1 has an integrated Pf4 prophage in its genome, encoding a relatively well-characterized filamentous phage, which contributes to the bacterial biofilm organization and maturation. Pf4 variants are considered as superinfectives when they can re-infect and kill the prophage-carrying host. Herein, the response of P. aeruginosa H103 to Pf4 variant infection was investigated. This phage variant caused partial lysis of the bacterial population and modulated H103 physiology. We show by confocal laser scanning microscopy that a Pf4 variant-infection altered P. aeruginosa H103 biofilm architecture either in static or dynamic conditions. Interestingly, in the latter condition, numerous cells displayed a filamentous morphology, suggesting a link between this phenotype and flow-related forces. In addition, Pf4 variant-infection resulted in cell envelope stress response, mostly mediated by the AlgU and SigX extracytoplasmic function sigma factors (ECFσ). AlgU and SigX involvement may account, at least partly, for the enhanced expression level of genes involved in the biosynthesis pathways of two matrix exopolysaccharides (Pel and alginates) and bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) metabolism

Caenorhabditis elegans: a model to monitor bacterial air quality

<p>Abstract</p> <p>Background</p> <p>Low environmental air quality is a significant cause of mortality and morbidity and this question is now emerging as a main concern of governmental authorities. Airborne pollution results from the combination of chemicals, fine particles, and micro-organisms quantitatively or qualitatively dangerous for health or for the environment. Increasing regulations and limitations for outdoor air quality have been decreed in regards to chemicals and particles contrary to micro-organisms. Indeed, pertinent and reliable tests to evaluate this biohazard are scarce. In this work, our purpose was to evaluate the <it>Caenorhaditis elegans </it>killing test, a model considered as an equivalent to the mouse acute toxicity test in pharmaceutical industry, in order to monitor air bacterial quality.</p> <p>Findings</p> <p>The present study investigates the bacterial population in dust clouds generated during crop ship loading in harbor installations (Rouen harbor, Normandy, France). With a biocollector, airborne bacteria were impacted onto the surface of agar medium. After incubation, a replicate of the colonies on a fresh agar medium was done using a velvet. All the replicated colonies were pooled creating the "Total Air Sample". Meanwhile, all the colonies on the original plate were isolated. Among which, five representative bacterial strains were chosen. The virulence of these representatives was compared to that of the "Total Air Sample" using the <it>Caenorhaditis elegans </it>killing test. The survival kinetic of nematodes fed with the "Total Air Sample" is consistent with the kinetics obtained using the five different representatives strains.</p> <p>Conclusions</p> <p>Bacterial air quality can now be monitored in a one shot test using the <it>Caenorhaditis elegans </it>killing test.</p

Pseudomonas aeruginosa Biofilm Dispersion by the Human Atrial Natriuretic Peptide

This is the final version. Available on open access from Wiley via the DOI in this recordData Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.Pseudomonas aeruginosa biofilms cause chronic, antibiotic tolerant infections in wounds and lungs. Numerous recent studies demonstrate that bacteria can detect human communication compounds through specific sensor/receptor tools that modulate bacterial physiology. Consequently, interfering with these mechanisms offers an exciting opportunity to directly affect the infection process. It is shown that the human hormone Atrial Natriuretic Peptide (hANP) both prevents the formation of P. aeruginosa biofilms and strongly disperses established P. aeruginosa biofilms. This hANP action is dose-dependent with a strong effect at low nanomolar concentrations and takes effect in 30-120 min. Furthermore, although hANP has no antimicrobial effect, it acts as an antibiotic adjuvant. hANP enhances the antibiofilm action of antibiotics with diverse modes of action, allowing almost full biofilm eradication. The hANP effect requires the presence of the P. aeruginosa sensor AmiC and the AmiR antiterminator regulator, indicating a specific mode of action. These data establish the activation of the ami pathway as a potential mechanism for P. aeruginosa biofilm dispersion. hANP appears to be devoid of toxicity, does not enhance bacterial pathogenicity, and acts synergistically with antibiotics. These data show that hANP is a promising powerful antibiofilm weapon against established P. aeruginosa biofilms in chronic infections.Normandy RegionFrench Ministry of Research (MRE

The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level

OprF is the major outer membrane porin in bacteria belonging to the Pseudomonas genus. In previous studies, we have shown that OprF is required for full virulence expression of the opportunistic pathogen Pseudomonas aeruginosa. Here, we describe molecular insights on the nature of this relationship and report that the absence of OprF leads to increased biofilm formation and production of the Pel exopolysaccharide. Accordingly, the level of c-di-GMP, a key second messenger in biofilm control, is elevated in an oprF mutant. By decreasing c-di-GMP levels in this mutant, both biofilm formation and pel gene expression phenotypes were restored to wild-type levels. We further investigated the impact on two small RNAs, which are associated with the biofilm lifestyle, and found that expression of rsmZ but not of rsmY was increased in the oprF mutant and this occurs in a c-di-GMP-dependent manner. Finally, the extracytoplasmic function (ECF) sigma factors AlgU and SigX displayed higher activity levels in the oprF mutant. Two genes of the SigX regulon involved in c-di-GMP metabolism, PA1181 and adcA (PA4843), were up-regulated in the oprF mutant, partly explaining the increased c-di-GMP level. We hypothesized that the absence of OprF leads to a cell envelope stress that activates SigX and results in a c-di-GMP elevated level due to higher expression of adcA and PA1181. The c-di-GMP level can in turn stimulate Pel synthesis via increased rsmZ sRNA levels and pel mRNA, thus affecting Pel-dependent phenotypes such as cell aggregation and biofilm formation. This work highlights the connection between OprF and c-di-GMP regulatory networks, likely via SigX (ECF), on the regulation of biofilm phenotypes

Caractérisation du microclimat sous petit tunnel et bâche à plat

The goal of this experiment is to develop a device and a measurement method adapted to this type of cover, to characterize a maximum of climatic parameters during the main phases of utilization, to characterize soil temperatures as a function of the covered width in order to optimize planting out dates to come up with a cultivation method under small tunnels and flat forcing frames. / L'objectif de cette expérimentation était de mettre au point un dispositif et une méthode de mesure adaptés à ce genre d'abri, de caractériser le maximum de paramètres climatiques pendant les principales phases d'utilisation, de caractériser les températures du sol en fonction de la largeur couverte en vue d'optimiser les dates de repiquages pour déduire un mode de culture sous petit tunnels ou bâches à plat

Plaques rigides à parois simples ou multiples pour serres horticoles - Norme NF T 54-406

This paper characterizes plastic rigid walls for covering greenhouses and defines test methods and various classes of level of quality. It contents - a main part (art - 5 and 6) establishing criteria and classes - an annex part giving characteric figures for each material, with the testing methods. / Ce document définit les prescriptions applicables aux plaques rigides en matières plastiques, à parois simples ou multiples destinées à la couverture de serres. Il indique les méthodes d'essais et fixe les classes correspondant aux différents niveaux d'exigence. Il comprend : - un volet principal (article 5 et 6) établissant les critères et classes ; ce volet est commun à tous les types de plaques utilisées ainsi qu'aux méthodes d'essai correspondantes ; - un volet annexe (article 7) donnant des valeurs pour les caractéristiques spécifiques à chaque matériau fini, avec les méthodes d'essais correspondantes

Mise en place d'une plateforme d'essai et d'étude pour la technologie des serres

In order to help industrials, technical centers, scientists, a greenhouse technology station has hean built, as well as those already existing in UK (Silsoe), Israel (Bet Dagan), Germany (Hanover) and Holland (Wageningen). / Il s'agit de mettre à la disposition des industriels, des chercheurs et technologues des serres et cultures sous abris, des outils d'expérimentation et de mesures adaptés à la technologie des serres. On ne cherche en aucun cas installer de vastes surfaces couvertes pour faire des essais en vraie grandeur et sur cultures : cela existe déjà dans la région (CTIFL à Balandran dans le Gard, INRA à Alénya dans les Pyrénées Orientales). On souhaite plutôt voir concentrer en un même lieu compétence pluridisciplinaire, matériel de mesure en laboratoire et in situ, possibilité de simulation radiative et d'ambiance, mini serres d'essais comparatifs, outils de calcul performants : et ce pour que toute idée de technique nouvelle puisse être conçue, analysée, fabriquée, simulée, testée en laboratoire puis en mini serre à l'extérieur, mesurée, modifiée, etc... Il existe une station de ce type en Hollande (Wageningen), une en Allemagne (Hanovre), une en Grande Bretagne (Silsoe), une en Israel (Bet Dagan). Le présent compte rendu fait donc état de l'avancement des travaux de construction et de mise en place de cette station, au 1er aril 1987