Flow injection determination of readily assimilable nitrogen compounds during vinification

A flow injection method for the determination of readily assimilable nitrogen (r.a.n.), i.e. ammonium and aminated nitrogen, is reported. The difference in pH of the sample in the presence and absence of formaldehyde, which blocks the amino function, provides the value of r.a.n. by monitoring the changes in absorbance of bromothymol blue at 616 nm. The detection and quantification limits are 10 and 11.6 mg l-1, respectively; the reproducibility and repeatability are 3.94 mg l-1 and 1.35 mg l-1, respectively; and the sample throughput is 20 samples h-1. The method has been applied to the analysis of 120 samples of must and wine subjected to biological aging. The proposed method also provides good correlation with the reference method used in routine analysis, and it is faster and gives sufficient precision for wineries requirements

LRP10, PGK1 and RPLP0: best reference genes in periprostatic adipose tissue under obesity and prostate cancer conditions

Obesity (OB) is a metabolic disorder characterized by adipose tissue dysfunction that has emerged as a health problem of epidemic proportions in recent decades. OB is associated with multiple comorbidities, including some types of cancers. Specifically, prostate cancer (PCa) has been postulated as one of the tumors that could have a causal relationship with OB. Particularly, a specialized adipose tissue (AT) depot known as periprostatic adipose tissue (PPAT) has gained increasing attention over the last few years as it could be a key player in the pathophysiological interaction between PCa and OB. However, to date, no studies have defined the most appropriate internal reference genes (IRGs) to be used in gene expression studies in this AT depot. In this work, two independent cohorts of PPAT samples (n = 20/n = 48) were used to assess the validity of a battery of 15 literature-selected IRGs using two widely used techniques (reverse transcription quantitative PCR [RT-qPCR] and microfluidic-based qPCR array). For this purpose, ΔCt method, GeNorm (v3.5), BestKeeper (v1.0), NormFinder (v.20.0), and RefFinder software were employed to assess the overall trends of our analyses. LRP10, PGK1, and RPLP0 were identified as the best IRGs to be used for gene expression studies in human PPATs, specifically when considering PCa and OB conditions

Mode Converter and Multiplexer with a Subwavelength Phase Shifter for Extended Broadband Operation

4 pags., 3 figs., 1 tab.On-chip mode converters and multiplexers are fundamental components to scale the capacity of silicon optical interconnects by using different spatial modes of waveguides. Recently, we proposed a low loss and compact mode converter and multiplexer consisting of a subwavelength-engineered multimode interference coupler, tapered waveguides as phase shifter and a symmetric Y-junction. However, the narrow spectral response of the tapered phase shifter limited the device crosstalk performance. In this work, we demonstrate that the use of a subwavelength grating phase shifter with low phase-shift errors substantially reduces the crosstalk and expands the operational bandwidth. A complete multiplexer-demultiplexer link consisting of two devices in back-to-back configuration was fabricated in a 220-nm silicon-on-insulator platform. Experimental measurements of the complete link show insertion loss below 2 dB and crosstalk less than -17 dB over a bandwidth of 245 nm (1427 - 1672 nm).is work was supported in part by the Spanish Ministry of Science and Innovation (MICINN) under grants RTI2018- 097957-B-C33, RED2018-102768-T, TEC2015-71127-C2-1-R (FPI BES- 2016-077798) and NEOTEC-CDTI-SNEO20181232 (Alcyon Photonics S.L.); and the Community of Madrid – FEDER funds (S2018/NMT-4326). This project has received funding from the Horizon 2020 research and innovation program under Marie Sklodowska-Curie grant No. 73433

A comprehensive dataset of annotated brain metastasis MR images with clinical and radiomic data.

Brain metastasis (BM) is one of the main complications of many cancers, and the most frequent malignancy of the central nervous system. Imaging studies of BMs are routinely used for diagnosis of disease, treatment planning and follow-up. Artificial Intelligence (AI) has great potential to provide automated tools to assist in the management of disease. However, AI methods require large datasets for training and validation, and to date there have been just one publicly available imaging dataset of 156 BMs. This paper publishes 637 high-resolution imaging studies of 75 patients harboring 260 BM lesions, and their respective clinical data. It also includes semi-automatic segmentations of 593 BMs, including pre- and post-treatment T1-weighted cases, and a set of morphological and radiomic features for the cases segmented. This data-sharing initiative is expected to enable research into and performance evaluation of automatic BM detection, lesion segmentation, disease status evaluation and treatment planning methods for BMs, as well as the development and validation of predictive and prognostic tools with clinical applicability

Efficacy and safety of the combination of reduced duration prophylaxis followed by immuno-guided prophylaxis to prevent cytomegalovirus disease in lung transplant recipients (CYTOCOR STUDY) : An open-label, randomised, non-inferiority clinical trial

Introduction Prolonged use of antivirals to prevent the development of cytomegalovirus (CMV) disease in lung transplant patients has been shown to have significant side effects, for which alternatives are being sought to reduce their use. The monitoring of cell immunity against CMV could be an alternative as it has shown to be useful in identifying transplant patients at low risk of infection, who could benefit from shorter prophylaxis. The aim of the CYTOCOR study is to demonstrate that the combination of a reduced prophylaxis strategy with subsequent CMV-specific immunological monitoring would allow CMV infection to be controlled in lung transplant patients as effectively as the usual strategy (prophylaxis followed by pre-emptive therapy), while reducing the side effects of antivirals due to the shorter duration of prophylaxis. Methods and analysis Phase III randomised, open, multicentre, parallel, non-inferiority clinical trial to study the efficacy and safety of the combination of a prophylaxis strategy up to month +3 post-transplant followed by immuno-guided prophylaxis using the QuantiFERON-CMV technique up to month +12 post-transplant to prevent CMV disease in CMV-seropositive lung transplant recipients. This strategy will be compared with a combination of a usual prophylaxis strategy up to month +6 post-transplant followed by pre-emptive therapy up to month +12. To study the incidence of CMV disease, patients will be followed up to 18 months post-transplantation. A total of 150 patients are expected to be recruited for the study. Ethics and public dissemination The clinical trial has been approved by the Research Ethics Committees and authorised by the Spanish Agency of Medicines and Medical Devices (AEMPS). If the hypothesis of this clinical trial is verified, the dissemination of the results could change clinical practice by increasing knowledge about the safety and efficacy of discontinuing valganciclovir prophylaxis in lung transplant recipients. Trial registration number NCT03699254

Efficacy and safety of the combination of reduced duration prophylaxis followed by immuno-guided prophylaxis to prevent cytomegalovirus disease in lung transplant recipients (CYTOCOR STUDY): an open-label, randomised, non-inferiority clinical trial.

INTRODUCTION: Prolonged use of antivirals to prevent the development of cytomegalovirus (CMV) disease in lung transplant patients has been shown to have significant side effects, for which alternatives are being sought to reduce their use. The monitoring of cell immunity against CMV could be an alternative as it has shown to be useful in identifying transplant patients at low risk of infection, who could benefit from shorter prophylaxis. The aim of the CYTOCOR study is to demonstrate that the combination of a reduced prophylaxis strategy with subsequent CMV-specific immunological monitoring would allow CMV infection to be controlled in lung transplant patients as effectively as the usual strategy (prophylaxis followed by pre-emptive therapy), while reducing the side effects of antivirals due to the shorter duration of prophylaxis. METHODS AND ANALYSIS: Phase III randomised, open, multicentre, parallel, non-inferiority clinical trial to study the efficacy and safety of the combination of a prophylaxis strategy up to month +3 post-transplant followed by immuno-guided prophylaxis using the QuantiFERON-CMV technique up to month +12 post-transplant to prevent CMV disease in CMV-seropositive lung transplant recipients. This strategy will be compared with a combination of a usual prophylaxis strategy up to month +6 post-transplant followed by pre-emptive therapy up to month +12. To study the incidence of CMV disease, patients will be followed up to 18 months post-transplantation. A total of 150 patients are expected to be recruited for the study. ETHICS AND PUBLIC DISSEMINATION: The clinical trial has been approved by the Research Ethics Committees and authorised by the Spanish Agency of Medicines and Medical Devices (AEMPS).If the hypothesis of this clinical trial is verified, the dissemination of the results could change clinical practice by increasing knowledge about the safety and efficacy of discontinuing valganciclovir prophylaxis in lung transplant recipient

Subwavelength silicon photonics: keynote presentation

2020 Photonics North (PN), Niagara Falls, ON, Canada, 26-28 May 2020Subwavelength structures are enabling a host of high-performances devices in the silicon photonic platform. Here we review our progress in the field, with an emphasis on the auspicious anisotropic properties of these structures for applications ranging from broadband on-chip GRIN-lenses, to zero-birefringence waveguides and polarization splitters

Analytical methods in wineries: is it time to change?

A review of the methods for the most common parameters determined in wine—namely, ethanol, sulfur dioxide, reducing sugars, polyphenols, organic acids, total and volatile acidity, iron, soluble solids, pH, and color—reported in the last 10 years is presented here. The definition of the given parameter, official and usual methods in wineries appear at the beginning of each section, followed by the methods reported in the last decade divided into discontinuous and continuous methods, the latter also are grouped in nonchromatographic and chromatographic methods because of the typical characteristics of each subgroup. A critical comparison between continuous and discontinuous methods for the given parameter ends each section. Tables summarizing the features of the methods and a conclusions section may help users to select the most appropriate method and also to know the state-of-the-art of analytical methods in this area

Silicon photonic mode multiplexers based on subwavelength metamaterials and on-chip beam forming

4 pags., 1 fig. -- Event: SPIE OPTO, 2023, San Francisco, California, United StatesIntegration of photonic circuits on silicon offers a unique opportunity to address the scaling of inter- and intra-chip communications in an energy-efficient and cost-effective manner. Mode-division multiplexing (MDM) is deemed as one of the most promising technologies to increase aggregated data bandwidth and avoid a communication capacity crunch. In this invited talk, we review our latest advances on integrated silicon mode multiplexers, including new topologies based on subwavelength grating (SWG) metamaterials for extended broadband operation and higher-order mode support. Specifically, we report on an ultra-broadband multiplexer based on a phase shifter and a multimode interference (MMI) coupler both engineered with subwavelength metamaterials. Experimental measurements of a complete multiplexer-demultiplexer link show losses lower than 2 dB and crosstalk below -17 dB over a bandwidth of 245 nm (1427 – 1672 nm).This work has been funded in part by the French Industry Ministry (Nano2022 project under IPCEI program); the Agence Nationale de la Recherche (ANR-MIRSPEC-17- CE09-0041); the European Union’s Horizon Europe (Marie SklodowskaCurie grant agreement Nº 101062518); the Spanish Ministry of Science and Innovation (MICINN) under grants RTI2018-097957-B-C33 and PID2020-115353RA-I00; the Spanish State Research Agency under grant MCIN/AEI/10.13039/501100011033 (PTQ2021-011974); the Community of Madrid – FEDER funds (S2018/NMT4326); the European Union – NextGenerationEU through the Recovery, Transformation and Resilience Plan (DIN2020-011488). The fabrication of the device was performed at the Plateforme de Micro-NanoTechnologie/C2N, which is partially funded by the Conseil General de l’Essonne. This work was partly supported by the French RENATECH network.Peer reviewe

Operation Allelopathy: An Experiment Investigating an Alternative to Synthetic Agrochemicals

Synthetic herbicides represent a serious problem in modern agriculture because they are not biodegradable and can accumulate in the soil and in the groundwater, a situation that allows them to enter the trophic chain and ultimately leads to human exposure. Allelopathic chemicals offer an effective alternative to the synthetic compounds. The aim of this experiment is to highlight the differences between chemical and biological control of diseases in crops and to demonstrate the use of natural fungicides and herbicides as alternatives to synthetic chemicals. The experiment involves an evaluation by students of the potential of plant extracts as an alternative to synthetic agrochemicals to identify new ecological farming techniques that could be applied in agriculture. In an effort to encourage 1800 students in high school (14 to 16 years old) to have an interest in science, technology, and innovation, the third edition of the Summer Science Campus was organized by the Spanish Foundation for Science and Technology and the Ministry of Education, Culture, and Sports with support from the foundation Obra Social “la Caixa”