Outer membrane vesicles from cold-adapted antarctic bacteria

Podeu consultar el llibre complet a: http://hdl.handle.net/2445/63704Many Gram-negative, cold-adapted bacteria from the Antarctic environment produce large amounts of extracellular matter with potential biotechnological applications. Transmission electron microscopy (TEM) analysis after high-pressure freezing and freeze substitution (HPF-FS) showed that this extracellular matter is structurally complex, appearing around cells as a netlike mesh, and composed of an exopolymeric substance (EPS) containing large numbers of outer membrane vesicles (OMVs). Isolation, purification and protein profiling via 1D SDS-PAGE confirmed the outer membrane origin of these Antarctic bacteria OMVs. In an initial attempt to elucidate the role of OMVs in cold-adapted strains of Gram-negative bacteria, a proteomic analysis demonstrated that they were highly enriched in outer membrane proteins and periplasmic proteins associated with nutrient processing and transport, suggesting that the OMVs may be involved in nutrient sensing and bacterial survival. OMVs from Gram-negative bacteria are known to play a role in lateral DNA transfer, but the presence of DNA in these vesicles has remained difficult to explain. A structural study of Shewanella vesiculosa M7T using TEM and Cryo-TEM revealed that this Antarctic Gram-negative bacterium naturally releases conventional one-bilayer OMVs, together with a more complex type of OMV, previously undescribed, which on formation drags along inner membrane and cytoplasmic content and can therefore also entrap DNA

Investigación sobre integración educativa de la aplicación Minecraft Education Edition.

La presente investigación tiene como objetivo estudiar la integración pedagógica de la aplicación Minecraft Education Edition. El estudio consistió en la aplicación del conocido videojuego Minecraft en su edición educativa a un grupo de 20 estudiantes de un centro escolar de la isla de Tenerife. Los objetivos de la investigación pretendieron conocer el grado de adquisición de competencia matemática y relacionadas con la inclusión, así como contrastar los conocimientos y opiniones iniciales del alumnado antes y después de realizar las sesiones del Minecraft Education Edition. Tras los análisis descriptivos llevados a cabo se obtuvo que los resultados en competencias matemáticas relacionados con el “saber” fueron alcanzados por la mayoría de los estudiantes con valores máximos, al igual que los relacionados con el “saber ser” vinculados a la inclusión. De otra parte, se obtuvieron diferencias en el antes y después de las sesiones en dimensiones como el control de las emociones y la autorregulación, así como en el uso de los videojuegos. Se concluye en la importancia del empleo de las nuevas tecnologías para una enseñanza motivadora y efectiva del alumnado en las primeras etapas.This research aims to study the pedagogical integration of the Minecraft: Education Edition application. The research consisted in the application of the well-known Minecraft videogame in its educational edition to a group of 20 students from a school in Tenerife island. The objectives of this research sought to know the level of acquisition of mathematical competence and related to the inclusion, as well as contrasting the initial knowledge and opinions of the students before and after performing the Minecraft: Education Edition lessons. After the descriptive analyses carried out, the results obtained in mathematical competences related to “know” were reached by most students with maximum values, as wells as those related to “know how to be” linked to inclusion. On the other hand, differences were obtained in the before and after lessons in dimensions such as emotional self-control an selfregulation, as well as the use of video games. It is concluded in the important of the use of new technologies for a motivating and effective teaching to students in the early stages

The overembeddedness impact on innovation: the small tourism ventures of the Comarca Minera, Hidalgo, Mexico

El objetivo es identificar las limitantes que la sobre integración social (overembeddedness) impone sobre las acciones de innovación entre los emprendedores que conforman el parque ecoturístico “El Bosque de las Truchas”, ubicado en la Comarca Minera, Hidalgo. Fueron analizados 23 emprendimientos turísticos. Los temas redundantes en las entrevistas fueron codificados y analizados mediante el software Atlas.ti y el cálculo del coeficiente C. Las limitaciones a la innovación derivan de la centralidad de la Asamblea de Ejidatarios que mediante el control de la información y de las relaciones con el exterior, ha propiciado un “bloqueo cognitivo” reflejado en una oferta turística repetitiva.The aim is to identify the constraints of the overembeddedness imposes on innovation actions that entrepreneurs want to develop in the “El Bosque de Las Truchas” ecotourism park, which is located in the Comarca Minera, Hidalgo. Twenty-three small tourism ventures were analyzed. The redundant topics in the interviews were coded and the relations between them were analyzed using the Atlas.ti software and the C coefficient. The Ejidatarios Assembly controls the flow of useful inside information as well as the exchange relations with the outside, which results in a “cognitive lock-in” that inhibits the innovative capacity of the entrepreneurship

Abordaje de la comunicación en un caso de afasia global

Global aphasia is an acquired language disorder that seriously affects comprehension, language expression, reading and writing, showing a right hemiplegia. Because of this inability to process language and these motor difficulties, global aphasia is the worst prognosis aphasia. Sometimes patient’s rehabilitation abilities to take advantage of rehabilitation and to restore a functional communicative interaction with the environment are underestimated. Here we report a case in which a patient with global aphasia comes in the healthcare service of the Pontificia University of Salamanca after an unsuccessful period of traditional rehabilitation and recovers some communication skills through training in communication through signs.La afasia global es un trastorno adquirido del lenguaje que afecta gravemente a la expresión, la comprensión, la lectura y escritura, cursando además con una hemiplejia derecha. Esta imposibilidad para el procesamiento del lenguaje y las dificultades motrices la convierte en la afasia de peor pronóstico. de esta forma, en algunas ocasiones se sibestiman las capacidades del enfermo para aprovechar la rehabilitación y poder reestablecer una interacción comunicativa funcional con el entorno. presentamos un caso en el que un paciente con afasia global acude al ServicioAsistencial de la Universidad Pontificia de Salamanca. TRas un periodo de rehabilitación tradicional, sin éxito, logra recuperar cierta capacidad de comunicación a través de un entrenamiento en comunicación signada

Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.

Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicle

Enhancement of soybean nodulation by seed treatment with non–thermal plasmas

Soybean (Glycine max (L.) Merrill) is one of the most important crops worldwide providing dietary protein and vegetable oil. Most of the nitrogen required by the crop is supplied through biological N2 fixation. Non-thermal plasma is a fast, economical, and environmental-friendly technology that can improve seed quality, plant growth, and crop yield. Soybean seeds were exposed to a dielectric barrier discharge plasma operating at atmospheric pressure air with superimposed flows of O2 or N2 as carrying gases. An arrangement of a thin phenolic sheet covered by polyester films was employed as an insulating barrier. We focused on the ability of plasma to improve soybean nodulation and biological nitrogen fixation. The total number of nodules and their weight were significantly higher in plants grown from treated seeds than in control. Plasma treatments incremented 1.6 fold the nitrogenase activity in nodules, while leghaemoglobin content was increased two times, indicating that nodules were fixing nitrogen more actively than control. Accordingly, the nitrogen content in nodules and the aerial part of plants increased by 64% and 23%, respectively. Our results were supported by biometrical parameters. The results suggested that different mechanisms are involved in soybean nodulation improvement. Therefore, the root contents of isoflavonoids, glutathione, auxin and cytokinin, and expansin (GmEXP1) gene expression were determined. We consider this emerging technology is a suitable pre-sowing seed treatment.Fil: Pérez Pizá, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Cejas, Ezequiel. Universidad Tecnológica Nacional. Facultad Reg.venado Tuerto. Departamento de Ing.electromecanica. Laboratorio de Descargas Eléctricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Zilli, Carla Giannina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Prevosto, Leandro. Universidad Tecnológica Nacional. Facultad Reg.venado Tuerto. Departamento de Ing.electromecanica. Laboratorio de Descargas Eléctricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Mancinelli, Beatriz Rosa. Universidad Tecnológica Nacional. Facultad Reg.venado Tuerto. Departamento de Ing.electromecanica. Laboratorio de Descargas Eléctricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Santa Cruz, Diego Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentin

Membrane vesicles released by a hypervesiculating Escherichia coli Nissle 1917 tolR mutant are highly heterogeneous and show reduced capacity for epithelial cell interaction and entry.

Membrane vesicles (MVs) produced by Gram-negative bacteria are being explored for novel clinical applications due to their ability to deliver active molecules to distant host cells, where they can exert immunomodulatory properties. MVs released by the probiotic Escherichia coli Nissle 1917 (EcN) are good candidates for testing such applications. However, a drawback for such studies is the low level of MV isolation from in vitro culture supernatants, which may be overcome by the use of mutants in cell envelope proteins that yield a hypervesiculation phenotype. Here, we confirm that a tolR mutation in EcN increases MV production, as determined by protein, LPS and fluorescent lipid measurements. Transmission electron microscopy (TEM) of negatively stained MVs did not reveal significant differences with wild type EcN MVs. Conversely, TEM observation after high-pressure freezing followed by freeze substitution of bacterial samples, together with cryo-TEM observation of plunge-frozen hydrated isolated MVs showed considerable structural heterogeneity in the EcN tolR samples. In addition to common one-bilayer vesicles (OMVs) and the recently described double-bilayer vesicles (O-IMVs), other types of MVs were observed. Time-course experiments of MV uptake in Caco-2 cells using rhodamine- and DiO-labelled MVs evidenced that EcN tolR MVs displayed reduced internalization levels compared to the wild-type MVs. The low number of intracellular MVs was due to a lower cell binding capacity of the tolR-derived MVs, rather than a different entry pathway or mechanism. These findings indicate that heterogeneity of MVs from tolR mutants may have a major impact on vesicle functionality, and point to the need for conducting a detailed structural analysis when MVs from hypervesiculating mutants are to be used for biotechnological applications

Toallas higiénicas ecológicas para adolescentes

El plan de marketing sobre la toalla higiénica ecológica Fresh To Go, busca atender una necesidad oculta en las adolescentes y sus madres, la adaptación a los cambios es una competencia que se valora mucho en estos tiempos y es ahí donde se busca atender y entender al consumidor a través de un producto que no solo responda de manera funcional sino también emocional. El objetivo de este proyecto es atender a un segmento específico para luego ir creciendo en el desarrollo de productos que mantengan como propósito: “conocer el mundo interno de la mujer para mejorar sus experiencias en las diferentes etapas de su vida”. Para sustentar la hipótesis, sobre la necesidad en el segmento, realizamos una investigación exploratoria, donde se pudo encontrar algunos insights importantes que denotan ciertos rasgos conductuales de las adolescentes que no son atendidos convenientemente por los productos que actualmente se vienen ofertando en el mercado. El principal objetivo es ser reconocidos como una marca diferente, innovadora y que se ocupa de las necesidades de la mujer en las diferentes fases de su vida. Llevar adelante este emprendimiento con propósito es parte de la naturaleza de Fresh To Go, la investigación y el conocimiento permitirá acompañar de cerca al público objetivo, se considera que lograr que la marca sea reconocida en el mercado, permitirá tomar las mejores decisiones empresariales para los accionistas y grupo de interés. El proyecto tiene un cronograma de actividades en un inicio por cinco años, donde se espera lograr los objetivos trazados y a partir del sexto año Fresh To Go se proyecta consolidar no solo en el segmento de adolescentes, sino también de mujeres de toda edad. Fresh To Go es una marca sin límites para mujeres sin límites.Trabajo de investigaciónCampus Lima Centr

Revealing the Complete Chloroplast Genome of an Andean Horticultural Crop, Sweet Cucumber (Solanum muricatum), and Its Comparison with Other Solanaceae Species

Sweet cucumber (Solanum muricatum) sect. Basarthrum is a neglected horticultural crop native of the Andean region. It is naturally distributed very close to other two Solanum crops of high importance, potatoes and tomatoes. To date, molecular tools for this crop are still undetermined. In this study, the complete sweet cucumber chloroplast (cp) genome was obtained and compared with seven Solanaceae species. The cp genome of S. muricatum had a 155,681 bp in length with included a large single copy (LSC) region of 86,182 bp and a small single-copy (SSC) region of 18,360 bp, separated by a pair of inverted repeats (IR) regions of 25,568 bp. The cp genome possessed 88 protein-coding genes (CDS), 37 transfer RNA (tRNA) genes, eight ribosomal RNA (rRNA) genes, and one pseudogene. Furthermore, 48 perfect microsatellites were identified, divided in mononucleotide repeats (32), followed by tetranucleotide (6) and dinucleotides (5). Microsatellites with trinucleotides repeats (3), pentanucleotide (1) and hexanucleotide (1) repeats motifs in these genomes were also identified, but in lower quantity. These repeats were mainly located in the noncoding regions. Whole cp genome comparative analysis revealed that the SSC and LSC regions showed more divergence than IR regions. Our phylogenetic analysis showed that S. muricatum is a sister species to members of sections Petota + Lycopersicum + Etuberosum. We expect to provide useful molecular data to shed light on the genetic diversity within sweet cucumber landrace, and also to determine the evolutionary processes in S. muricatum

Effects of non-thermal plasma technology on Diaporthe longicolla cultures and mechanisms involved

BACKGROUND: The Diaporthe/Phomopsis complex (D/P) is a group of soybean seed-borne fungi. The use of chemical fungicides, either for seed treatment or during the crop cycle, is the most adopted practice for treating fungal diseases caused by this complex. Worldwide, there is a search for alternative seed treatments that are less harmful to the environment than chemicals. Non-thermal plasma (NTP) is a novel seed treatment technology for pathogen removal. This research aimed to evaluate the effects of NTP on the in vitro performance of pure cultures of Diaporthe longicolla and elucidate the mechanisms underlying these effects. RESULTS: Active D. longicolla mycelium, growing in vitro, was exposed to different NTP treatments, employing a dielectric barrier discharge arrangement with different carrier gases (N2 or O2). Fungal growth, fresh biomass and colony appearance were negatively affected by plasma treatments (TN3 and TO3). Lipid peroxidation and antioxidant activities were higher in plasma-treated colonies comparison with non-exposed colonies (control). Fungal asexual spores (conidia) were also exposed to NTP, showing high susceptibility. CONCLUSION: Exposure of D. longicolla colonies to NTP severely compromised fungal biology. Ozone production during treatment and lipid peroxidation of fungal cell membranes appeared to be involved in the observed effects.Fil: Pérez Pizá, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaFil: Cejas, Ezequiel. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Departamento de Ingeniería Electromecánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chamorro Garcés, Juan Camilo. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Departamento de Ingeniería Electromecánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferreyra, Matías Germán. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Departamento de Ingeniería Electromecánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zilli, Carla Giannina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Vallecorsa, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Santa Cruz, Diego Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Prevosto, Leandro. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Departamento de Ingeniería Electromecánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentin