Eficacia de los niveles séricos de S100B, TRAIL y adropina para predecir el resultado clínico, el núcleo del infarto final y los subtipos de ataque cerebrovascular de los pacientes con accidente cerebrovascular isquémico agudo

Objectives: To identify the significance of TRAIL and adropin release and the relative changes related to S100B levels and the relationship between these biomarkers with final infarct core, clinical outcome, and the presence of large artery atherosclerosis in acute stroke patients. Material and methods: Over a one-year period, demographic, clinical, and neuroimaging findings of 90 consecutive patients with acute ischemic stroke were evaluated. Results: Among our study population, the mean age was 69.28 ± 10 and 39 patients were female. The increased level of S100B and the decreased levels of sTRAIL with adropin were significantly associated with the moderate to the severe patient neurologic presentation (p=.0001, p=.002, p =.002, respectively). On control CT, a large infarct core was significantly associated with decreased serum level of sTRAIL and adropin (p=.001 and p=.000; respectively); however, the levels of S100B were not significantly associated with good ASPECT score (p=.684). Disability and unfavorable outcome were significantly related to the decreased level of sTRAIL and adropin (p=.001 and p=.000; respectively for THRIVE score>5). Decreased sTRAIL and adropin levels and increased S100B level were correlated with the presence of large artery atherosclerotic etiologic factor among the study population (p=.000, p=.000, p =.036, respectively). Conclusion: TRAIL and Adropin serum levels are associated with poor clinical outcome and greater infarcted area in acute ischemic stroke patients.Objetivo. Identificar la importancia de la liberación de TRAIL y adropin y los cambios relativos relacionados con los niveles de S100B y la relación entre estos biomarcadores con el núcleo final del infarto, el resultado clínico y la presencia de aterosclerosis de arterias grandes en pacientes con ataque cerebrovascular agudo. Materiales y métodos. Durante un período de un año, se evaluaron los hallazgos demográficos, clínicos y de neuroimagen de 90 pacientes consecutivos con ataque cerebrovascular isquémico agudo. Resultados. Entre la población de nuestro estudio, la edad media fue de 69,28 ± 10 y 39 pacientes eran mujeres. El aumento del nivel de S100B y la disminución de los niveles de sTRAIL con adropina se asociaron significativamente con la presentación neurológica del paciente de moderada a grave (p = ,0001, p = ,002, p = ,002, respectivamente). En la TC de control, un gran núcleo de infarto se asoció significativamente con una disminución del nivel sérico de sTRAIL y adropina (p = ,001 y p = ,000; respectivamente); sin embargo, los niveles de S100B no se asociaron significativamente con una buena puntuación ASPECT (p = ,684). La discapacidad y el resultado desfavorable se relacionaron significativamente con la disminución del nivel de sTRAIL y adropina (p = ,001 yp = ,000; respectivamente para la puntuación THRIVE> 5). La disminución de los niveles de sTRAIL y adropina y el aumento del nivel de S100B se correlacionaron con la presencia de un factor etiológico aterosclerótico de arterias grandes entre la población de estudio (p = ,000, p = ,000, p = ,036, respectivamente). Conclusiones. Los niveles séricos de TRAIL y Adropin se asocian con un resultado clínico deficiente y una mayor área infartada en pacientes con ataque cerebrovascular isquémico agudo

The Importance of ACTN3 R577X Polymorphism in Athletic Performance and Modeling of R577X Mutant Type and Wild Type ACTN3 Protein by Bioinformatics Analysis

In studies conducted on the relationship between ACTN3 R577X polymorphism and athletic performance, it is known that athletic performance is polygenic, but the most sensitive relationship is provided by the ACTN3 gene. Considering the results, the conformational difference is much less (<0.05 A) between wild type and R577X. R577X has fewer amino acids (324 amino acids) than the wild type. It has been observed that the subunits of the R577X model do not have 4 long and 5 short alpha-helical curves. According to the analysis results of physico-chemistry properties, each model is very close to alipatich endex, but the R577X mutant type is higher than the wild type. Each model contains a large number of hydrophilic amino acids. R577 was found to be more hydrophilic than wild type. Each model has more likely amino acids versus negative amino acids. However, it considers the R577X mutant type more likely than the wild type. The purpose of this study is; explaining physico and chemistry properties with 3D homology between the wild type of ACTN gene and the R577X mutation region and contributed to future studies on this subject by making homology models. Uniprot, Swiss Model and Chimera were used as bioinformatics transition. Our results showed that there was no significant change in the molecular structure, in which case the function of the protein was not impaired. However, athletes with this mutation at the end of intense muscle performances are more susceptible to muscle damage. R577X mutant and wild-type protein of ACTN3 the physico-chemical properties of three-dimensional homology model was performed for the first time in Turkey

Protective Effect of Ischemic Preconditioning on Myocardium Against Remote Tissue Injury Following Transient Focal Cerebral Ischemia in Diabetic Rats

Abstract Background: Remote ischemic preconditioning (IPreC) could provide tissue-protective effect at a remote site by anti-inflammatory, neuronal, and humoral signaling pathways. Objectives: The aim of the study was to investigate the possible protective effects of remote IPreC on myocardium after transient middle cerebral artery occlusion (MCAo) in streptozotocin- induced diabetic (STZ) and non-diabetic rats. Methods: 48 male Spraque Dawley rats were divided into eight groups: Sham, STZ, IPreC, MCAo, IPreC+MCAo, STZ+IPreC, STZ+MCAo and STZ+IPreC+MCAo groups. We induced transient MCAo seven days after STZ-induced diabetes, and performed IPreC 72 hours before transient MCAo. Remote myocardial injury was investigated histopathologically. Bax, Bcl2 and caspase-3 protein levels were measured by Western blot analysis. Total antioxidant status (TAS), total oxidant status (TOS) of myocardial tissue were measured by colorimetric assay. Oxidative stress index(OSI) was calculated as TOS-to-TAS ratio. For all statistical analysis, p values < 0.05 were considered significant. Results: We observed serious damage including necrosis, congestion and mononuclear cell infiltration in myocardial tissue of the diabetic and ischemic groups. In these groups TOS and OSI levels were significantly higher; TAS levels were lower than those of IPreC related groups (p < 0.05). IPreC had markedly improved histopathological alterations and increased TAS levels in IPreC+MCAo and STZ+IPreC+MCAo compared to MCAo and STZ+MCAo groups (p < 0.05). In non-diabetic rats, MCAo activated apoptotic cell death via increasing Bax/Bcl2 ratio and caspase-3 levels. IPreC reduced apoptotic cell death by suppressing pro-apoptotic proteins. Diabetes markedly increased apoptotic protein levels and the effect did not reversed by IPreC. Conclusions: We could suggest that IPreC attenuates myocardial injury via ameliorating histological findings, activating antioxidant mechanisms, and inducing antiapoptotic activity in diabetic rats

Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway

Purpose: Our goal is to clarify the effectiveness of berberine (BBR) on cerebral vasospasm induced by subarachnoid hemorrhage

The Isolated Abducens Nerve Palsy Occured After Postviral Infection in A Diabetic Patient

WOS: 000449522300015Malignancy, immunosuppressive drug use, and diabetes mellitus (DM) are defined as risk factors for herpes zoster. A 58-year-old male patient with the diagnosis of DM was admitted to the emergency room with the complaints of double vision. His neurological examination revealed under activity of the right lateral rectus muscle and hypoactive deep tendon reflexes. In this report, we discuss a rare case of isolated abducens nerve palsy that occurred after postviral infection in the patient

The Effect of Perioperative Topical Ketorolac 0.5% on Macular Thickness After Uneventful Phacoemulsification

Background To evaluate the effects of topical 0.5% ketorolac treatment combined with topical steroids on macular thickness in cases who had uneventful phacoemulsification surgery. Methods 58 eyes of 58 consecutive cases were included. The mean foveal thickness (MFT), parafoveal thickness (ParaFT), and perifoveal thickness (PeriFT) measurements were performed with optical coherence tomography (RTVue-100, Optovue, Fremont, CA, USA) preoperatively and at postoperative 1 week, 1 month, and 2 months. All cases received topical 0.1% dexamethasone postoperatively. Randomly selected cases additionally received topical 0.5% ketorolac, which started 2 days prior to surgery. Cases who received both topical steroids and ketorolac formed group 1 and subjects who received only topical steroids formed group 2. Results The increase in mean MFT at the 1st week, 1st month, and 2nd months after surgery in group 1 was significantly lower than group 2 (P = 0.008, P ≤ 0.001, and P ≤ 0.001, resp.). In group 1, the increase in mean ParaFT and PeriFT was significantly lower than group 2 at the 1st and 2nd months of the surgery (P < 0.05 for all variables). Conclusions Topical ketorolac combined with steroids is highly efficacious in order to prevent increment in thickness on each part of the macula even after an uneventful phacoemulsification surgery comparing to steroid monotheraphy.PubMedWoSScopu

Current Status of Genetic Diagnosis Laboratories and Frequency of Genetic Variants Associated with Cystic Fibrosis through a Newborn-Screening Program in Turkey

Background: Cystic fibrosis (CF) is the most common worldwide, life-shortening multisystem hereditary disease, with an autosomal recessive inheritance pattern caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. The national newborn screening (NBS) program for CF has been initiated in Turkey since 2015. If the immunoreactive trypsinogen (IRT) is elevated (higher than 70 &mu;g/L in the second control) and confirmed by sweat test or clinical findings, genetic testing is performed. The aims of this study are to emphasize the effect of NBS on the status of genetic diagnosis centers with the increasing numbers of molecular testing methods, and to determine the numbers and types of CFTR mutations in Turkey. Methods: The next-generation sequencing (NGS) and multiplex ligation-dependent probe amplification (MLPA) results of 1595 newborns, who were referred to Cukurova University Adana Genetic Diseases Diagnosis and Treatment Center (AGENTEM) for molecular genetic testing, were evaluated with positive CF NBS program results since 2017. Results: According to the results; 560 (35.1%) of the 1595 patients carried at least 1 (one) CF-related variant, while 1035 patients (64.9%) had no mutation. Compound heterozygosity for two mutations was the most common in patients, while two detected variants were homozygote in 14 patients. A total of 161 variants were detected in 561 patients with mutations. Fifteen novel variants that have not been previously reported were found. Moreover, p.L997F was identified as the most frequent pathogenic mutation that might affect the IRT measurements used for the NBS. The distribution of mutation frequencies in our study showed a difference from those previously reported; for example, the well-known p.F508del was the third most common (n = 42 alleles), rather than the first. The most striking finding is that 313 cases had a pathogenic variant together with the V470M variant, which might have a cumulative effect on CF perpetuation. Conclusion: This study is the first to determine the mutational spectrum of CFTR in correlation with the NBS program in the Turkish population. NBS for CF raises issues regarding screening in diverse populations, both medical and non-medical benefits, and carrier identification. Through the lens of NBS, we focused on the integrated diagnostic algorithms and their effect on the results of genetic testing