3,434 research outputs found
Rearing Bumble Bees for Research and Profit: Practical and Ethical Considerations
The commercial production of bumble bee colonies is a multi-million dollar business worldwide. The pollination of greenhouse tomatoes is largely dependent on this industry. However, microparasites are prevalent in many of these colonies and can spread to wild populations of bumble bees. Academic researchers now commonly purchase colonies for their work. I believe that this raises some questions: (a) What is the danger of exacerbating the problem of spread of parasites and pathogens to wild population of bumble bees from field studies using purchased colonies? (b) How representative studies are done on only a few species, for example, B. terrestris, B. impatiens? (c) Does the purchase and use of these colonies give tacit approval to the industry, which may be having a detrimental effect on the native populations of bumble bees? This is an ethical issue. (d) Loss of âfeeling for the organismâ by researchers and particularly graduate students. These issues were discussed, and the classical method of bumble bee rearing which avoids these problems was described
Geographical, Entomological and Botanical Origins of Honey
The Codex Alimentarius Commission defines honey as: â⊠the natural sweet substance produced by honey bees from the nectar of plants ⊠which the bees collect, transform by combining with specific substances of their own, deposit, dehydrate, store and leave in the honey comb to ripen and matureâ. Honey, produced in all regions of the world varies widely in its chemical and physical properties, which depend on the plants the bees visit and on the species of Apis themselves. The Codex sets standards for the composition of honeys, levels of contaminants permitted, and the correct labelling according to floral source and geographic origin. The growth of stingless bee (Meliponidae) domestication in Central and South America, Asia and Australia has led to another significant source of honey, which is very variable in its properties. Here I review of the properties of honeys and the techniques used to analyze the geographical, entomological and botanical origins of honey, discuss some of the properties and features of the honeys made by the stingless bees, and discuss unusual honeys, the so-called âmad honeysâ, made from nectar containing toxic compounds, and the effect of toxic nectar on bees (bumble bees) and humans
Genetic and Morphometric Evidence for the Conspecific Status of the Bumble Bees, Bombus melanopygus and Bombus edwardsii
The taxonomic status of closely related bumble bee species is often unclear. The relationship between the two nominate taxa, Bombus melanopygus Nylander (Hymenoptera: Apidae) and Bombus edwardsii Cresson (Hymenoptera: Apidae), was investigated using genetic (enzyme electrophoretic) and morphometric analyses. The taxa differ in the color of the abdominal terga two and three, being ferruginous in B. melanopygus and black in B. edwardsii. B. edwardsii occurs throughout California, while B. melanopygus extends north through Oregon, to Alaska and Canada. They are sympatric only in southern Oregon and northern California. The taxonomic status of these taxa was questioned when Owen and Plowright (1980) reared colonies from queens collected in the area of sympatry, and discovered that pile coloration was due to a single, biallelic Mendelian gene, with the red (R) allele dominant to the black (r). Here it is shown that all the taxa, whether from California, Oregon, or Alberta, have the same electrophoretic profile and cannot be reliably distinguished by wing morphometrics. This strongly supports the conclusion that B. melanopygus and B. edwardsii are conspecific and should be synonymized under the name B. melanopygus. Hence, there is a gene frequency cline running from north to south, where the red allele is completely replaced by the black allele over a distance of about 600 km
The cluster Abell 780: an optical view
The Abell 780 cluster, better known as the Hydra A cluster, has been
thouroughly analyzed in X-rays. However, little is known on its optical
properties. We derive the galaxy luminosity function (GLF) in this apparently
relaxed cluster, and search for possible environmental effects by comparing the
GLFs in various regions, and by looking at the galaxy distribution at large
scale around Abell 780. Our study is based on optical images obtained with the
ESO 2.2m telescope and WFI camera in the B and R bands, covering a total region
of 67.22x32.94 arcmin^2, or 4.235x2.075 Mpc^2 for a cluster redshift of 0.0539.
In a region of 500 kpc radius around the cluster centre, the GLF in the R band
shows a double structure, with a broad and flat bright part and a flat faint
end that can be fit by a power law with an index alpha=-0.85+-0.12 in the
20.25<R<21.75 interval. If we divide this 500 kpc radius region in North+South
or East+West halves, we find no clear difference between the GLFs in these
smaller regions. No obvious large scale structure is apparent within 5 Mpc from
the cluster, based on galaxy redshifts and magnitudes collected from the NED
database in a much larger region than that covered by our data, suggesting that
there is no major infall of material in any preferential direction. However,
the Serna-Gerbal method reveals the presence of a gravitationally bound
structure of 27 galaxies, which includes the cD, and of a more strongly
gravitationally bound structure of 14 galaxies. These optical results agree
with the overall relaxed structure of Abell 780 previously derived from X-ray
analyses.Comment: Accepted for publication in Astronomy & Astrophysic
Automated eukaryotic gene structure annotation using EVidenceModeler and the Program to Assemble Spliced Alignments
EVidenceModeler (EVM) is an automated annotation tool that predicts protein-coding regions, alternatively spliced transcripts and untranslated regions of eukaryotic genes
Psychiatric disorders in children with 16p11.2 deletion and duplication
Deletion and duplication of 16p11.2 (BP4âBP5) have been associated with an increased risk of intellectual disability and psychiatric disorder. This is the first study to compare the frequency of a broad spectrum of psychiatric disorders in children with 16p11.2 deletion and duplication. We aimed to evaluate (1) the nature and prevalence of psychopathology associated with copy number variation (CNV) in children with 16p11.2 by comparing deletion and duplication carriers with family controls; (2) whether deletion and duplication carriers differ in frequency of psychopathology. 217 deletion carriers, 77 deletion family controls, 114 duplication carriers, and 32 duplication family controls participated in the study. Measures included standardized research diagnostic instruments. Deletion carriers had a higher frequency of any psychiatric disorder (OR = 8.9, p < 0.001), attention deficit hyperactivity disorder (ADHD) (OR = 4.0, p = 0.01), and autism spectrum disorder (ASD) (OR = 39.9, p = 0.01) than controls. Duplication carriers had a higher frequency of any psychiatric diagnosis (OR = 5.3, p = 0.01) and ADHD (OR = 7.0, p = 0.02) than controls. The prevalence of ASD in child carriers of deletions and duplications was similar (22% versus 26%). Comparison of the two CNV groups indicated a higher frequency of ADHD in children with the duplication than deletion (OR = 2.7, p = 0.04) as well as a higher frequency of overall psychiatric disorders (OR = 2.8, p = 0.02) and psychotic symptoms (OR = 4.7, p = 0.02). However, no differences between deletion and duplications carriers in the prevalence of ASD were found. Both deletion and duplication are associated with an increased risk of psychiatric disorder, supporting the importance of early recognition, diagnosis, and intervention in these groups
Best practice guidelines for cetacean tagging
Animal-borne electronic instruments (tags) are valuable tools for collecting information on cetacean physiology, behaviour and ecology, and for enhancing conservation and management policies for cetacean populations. Tags allow researchers to track the movement patterns, habitat use andother aspects of the behaviour of animals that are otherwise difficult to observe. They can even be used to monitor the physiology of a tagged animal within its changing environment. Such tags are ideal for identifying and predicting responses to anthropogenic threats, thus facilitating the development of robust mitigation measures. With the increasing need for data best provided by tagging and the increasing availability of tags, such research is becoming more common. Tagging can, however, pose risks to the health and welfare of cetaceans and to personnel involved in tagging operations. Here we provide âbest practiceâ recommendations for cetacean tag design, deployment and follow-up assessment of tagged individuals, compiled by biologists and veterinarians with significant experience in cetacean tagging. This paper is intended to serve as a resource to assist tag users, veterinarians, ethics committees and regulatory agency staff in the implementation of high standards of practice, and to promote the training of specialists in this area. Standardised terminology for describing tag design and illustrations of tag types and attachment sites are provided, along with protocols for tag testing and deployment (both remote and through capture-release), including training of operators. The recommendations emphasise the importance of ensuring that tagging is ethically and scientifically justified for a particular project and that tagging only be used to address bona fide research or conservation questions that are best addressed with tagging, as supported by an exploration of alternative methods. Recommendations are provided for minimising effects on individual animals (e.g. through careful selection of the individual, tag design and implant sterilisation) and for improving knowledge of tagging effects on cetaceans through increased post-tagging monitoring.Publisher PDFPeer reviewe
Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications
We have designed a novel non-antibody scaffold protein, termed Adhiron, based on a phytocystatin consensus sequence. The Adhiron scaffold shows high thermal stability (Tm ca. 101°C), and is expressed well in Escherichia coli. We have determined the X-ray crystal structure of the Adhiron scaffold to 1.75 Ă
resolution revealing a compact cystatin-like fold. We have constructed a phage-display library in this scaffold by insertion of two variable peptide regions. The library is of high quality and complexity comprising 1.3 Ă 10(10) clones. To demonstrate library efficacy, we screened against the yeast Small Ubiquitin-like Modifier (SUMO). In selected clones, variable region 1 often contained sequences homologous to the known SUMO interactive motif (V/I-X-V/I-V/I). Four Adhirons were further characterised and displayed low nanomolar affinities and high specificity for yeast SUMO with essentially no cross-reactivity to human SUMO protein isoforms. We have identified binders against >100 target molecules to date including as examples, a fibroblast growth factor (FGF1), platelet endothelial cell adhesion molecule (PECAM-1; CD31), the SH2 domain Grb2 and a 12-aa peptide. Adhirons are highly stable and well expressed allowing highly specific binding reagents to be selected for use in molecular recognition applications
Clinical Impact of Ceftriaxone Resistance in Escherichia coli Bloodstream Infections: A Multicenter Prospective Cohort Study
BACKGROUND: Ceftriaxone-resistant (CRO-R) Escherichia coli bloodstream infections (BSIs) are common.
METHODS: This is a prospective cohort of patients with E coli BSI at 14 United States hospitals between November 2020 and April 2021. For each patient with a CRO-R E coli BSI enrolled, the next consecutive patient with a ceftriaxone-susceptible (CRO-S) E coli BSI was included. Primary outcome was desirability of outcome ranking (DOOR) at day 30, with 50% probability of worse outcomes in the CRO-R group as the null hypothesis. Inverse probability weighting (IPW) was used to reduce confounding.
RESULTS: Notable differences between patients infected with CRO-R and CRO-S E coli BSI included the proportion with Pitt bacteremia score â„4 (23% vs 15%, P = .079) and the median time to active antibiotic therapy (12â
hours [interquartile range {IQR}, 1-35 hours] vs 1â
hour [IQR, 0-6 hours]; P \u3c .001). Unadjusted DOOR analyses indicated a 58% probability (95% confidence interval [CI], 52%-63%) for a worse clinical outcome in CRO-R versus CRO-S BSI. In the IPW-adjusted cohort, no difference was observed (54% [95% CI, 47%-61%]). Secondary outcomes included unadjusted and adjusted differences in the proportion of 30-day mortality between CRO-R and CRO-S BSIs (-5.3% [95% CI, -10.3% to -.4%] and -1.8 [95% CI, -6.7% to 3.2%], respectively), postculture median length of stay (8 days [IQR, 5-13 days] vs 6 days [IQR, 4-9 days]; P \u3c .001), and incident admission to a long-term care facility (22% vs 12%, P = .045).
CONCLUSIONS: Patients with CRO-R E coli BSI generally have poorer outcomes compared to patients infected with CRO-S E coli BSI, even after adjusting for important confounders
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