Spanish Validation of the Flourishing Scale in the General Population

Well-being research and its measurement have grown in the last two decades. The objective of this study was to adapt and validate the Flourishing Scale in a sample of Spanish adults. This was a cross-sectional study using a non-probabilistic sample of 999 Spanish general adult population participants. The psychometric properties of the scale were analysed from an exploratory and confirmatory perspective. Exploratory factor analysis showed a one-factor solution explaining 42.3% of the variance; an internal consistency of .846; temporal reliability correlation of .749; convergent validity with the Satisfaction with Life Scale of .521 and criterion validity with positive and negative affect (PANAS), pessimism and optimism (LOT-R) ranging from .270 to .488. Confirmatory factor analysis testing the one-factor solution showed a χ2 of 65.57 df = 20; CFI of .982, RMSEA of .06, average variance extracted index of .518 and composite reliability index of .841. Results showed that the Spanish version of the FS is a reliable and valid method for measuring high levels of well-bein

Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?

The severity of canine leishmaniosis (CanL) due to Leishmania infantum might be affected by other vector-borne organisms that mimic its clinical signs and clinicopathological abnormalities. The aim of this study was to determine co-infections with other vector-borne pathogens based on serological and molecular techniques in dogs with clinical leishmaniosis living in Spain and to associate them with clinical signs and clinicopathological abnormalities as well as disease severity. Sixty-one dogs with clinical leishmaniosis and 16 apparently healthy dogs were tested for Rickettsia conorii, Ehrlichia canis, Anaplasma phagocytophilum and Bartonella henselae antigens by the immunofluorescence antibody test (IFAT) and for E. canis, Anaplasma spp., Hepatozoon spp., Babesia spp. and filarioid DNA by polymerase chain reaction (PCR). Among the dogs examined by IFAT, the seroprevalences were: 69% for R. conorii, 57% for E. canis, 44% for A. phagocytophilum and 37% for B. henselae ; while the prevalences found by PCR were: 8% for Ehrlichia / Anaplasma, 3% for Anaplasma platys and 1% for H. canis. No other pathogen DNA was detected. Statistical association was found between dogs with clinical leishmaniosis and seroreactivity to R. conorii antigen (Fisher's exact test: P = 0.025, OR = 4.1, 95% CI = 1-17) and A. phagocytophilum antigen (Fisher's exact test: P = 0.002, OR = 14.3, 95% CI = 2-626) and being positive to more than one serological or molecular tests (co-infections) (Mann-Whitney test: U = 243, Z = -2.6, n = 14, n = 61, P = 0.01) when compared with healthy dogs. Interestingly, a statistical association was found between the presence of R. conorii, E. canis, A. phagocytophilum and B. henselae antibodies in sick dogs and some clinicopathological abnormalities such as albumin and albumin/globulin ratio decrease and increase in serum globulins. Furthermore, seroreactivity with A. phagocytophilum antigens was statistically associated with CanL clinical stages III and IV. This study demonstrates that dogs with clinical leishmaniosis from Catalonia (Spain) have a higher rate of co-infections with other vector-borne pathogens when compared with healthy controls. Furthermore, positivity to some vector-borne pathogens was associated with more marked clinicopathological abnormalities as well as disease severity with CanL

Suitable indicators using stem diameter variation-derived indices to monitor the water status of greenhouse tomato plants

It is very important to seek a simple nondestructive method to continuously measure plant water status for irrigation scheduling. Changes in stem diameter in response to plant water status and soil water content (SWC) were experimentally investigated during the growing seasons of 2011/2012 and 2012/2013 in pot-cultivated tomato (Lycopersicon esculentum L.) plants in a plastic greenhouse. This study was conducted to determine suitable SDV (stem diameter variation)-derived indices as indicators of tomato plant water status for irrigation scheduling. The experiment was designed as a two-factor randomized block using the SWC and growth stages as variables. The SWC was controlled at 70–80% (well-watered), 60–70% (slightly deficit watered), 50–60% (moderately deficit watered) of the field capacity (FC), and the prescribed growing stages were vegetative, flowering and fruit-forming, and harvesting stages. Regression analysis showed that the SD(6) (the difference between the stem diameter value at 06:00 am and the initial sensor reading) was closely related to the SWC (p<0.01) during rapid vegetative growth, whereas the MDS (the maximum daily shrinkage) was closely related to the SWC (p<0.01) during slow vegetative growth. Our results suggest that SDV-derived indicators can be used for determining plant water status and for scheduling irrigation at different growth/developmental stages

Automatic drought stress detection in grapevines without using conventional threshold values

Aims : Because the water status of grapevines strongly affects the quality of the grapes and resulting wine, automated and early drought stress detection is important. Plant measurements are very promising for detecting drought stress, but strongly depend on microclimatic changes. Therefore, conventional stress detection methods require threshold values which define when plants start sensing drought stress. There is however no unique method to define these values. In this study, we propose two techniques that overcome this limitation. Methods : Two statistical methods were used to automatically distinguish between drought and microclimate effects, based on a short preceding full-irrigated period to extract plant behaviour under normal conditions: Unfold Principal Component Analysis (UPCA) and Functional Unfold Principal Component Analysis (FUPCA). Both techniques aimed at detecting when measured sap flow rate or stem diameter variations in grapevine deviated from their normal behaviour due to drought stress. Results : The models based on sap flow rate had some difficulties to detect stress on days with low atmospheric demands, while those based on stem diameter variations did not show this limitation, but ceased detecting stress when the stem diameter levelled off after a period of severe shrinkage. Nevertheless, stress was successfully detected with both approaches days before visible symptoms appeared. Conclusions : UPCA and FUPCA based on plant indicators are therefore very promising for early stress detection

SIRT7 ワ Osterix ノ リジンアシルカ オ セイギョ スルコト デ コツケイセイ ニ オケル ジュウヨウ ナ ヤクワリ オ ニナウ

熊本大

Model-assisted evaluation of crop load effects on stem diameter variations and fruit growth in peach

Key message: The paper identifies and quantifies how crop load influences plant physiological variables that determine stem diameter variations to better understand the effect of crop load on drought stress indicators. Stem diameter (D (stem)) variations have extensively been applied in optimisation strategies for plant-based irrigation scheduling in fruit trees. Two D (stem) derived water status indicators, maximum daily shrinkage (MDS) and daily growth rate (DGR), are however influenced by other factors such as crop load, making it difficult to unambiguously use these indicators in practical irrigation applications. Furthermore, crop load influences the growth of individual fruits, because of competition for assimilates. This paper aims to explain the effect of crop load on DGR, MDS and individual fruit growth in peach using a water and carbon transport model that includes simulation of stem diameter variations. This modelling approach enabled to relate differences in crop load to differences in xylem and phloem water potential components. As such, crop load effects on DGR were attributed to effects on the stem phloem turgor pressure. The effect of crop load on MDS could be explained by the plant water status, the phloem carbon concentration and the elasticity of the tissue. The influence on fruit growth could predominantly be explained by the effect on the early fruit growth stages

A step towards new irrigation scheduling strategies using plant-based measurements and mathematical modelling

Because of the increasing worldwide shortage of freshwater and costs of irrigation, a new plant-based irrigation scheduling method is proposed. In this method, two real-time plant-based measurements (sap flow and stem diameter variations) are used in combination with a mathematical water flow and storage model in order to predict the stem water potential. The amount of required irrigation water is derived from a time integration of the sap flow profile, while the timing of the irrigation is controlled based on a reference value for the predicted stem water potential. This reference value is derived from the relationship between midday values of maximum photosynthesis rates and stem water potential. Since modelling is an important part of the proposed methodology, a thorough mathematical analysis (identifiability analysis) of the model was performed. This analysis showed that an initial (offline) model calibration was needed based on measurements of sap flow, stem diameter variation and stem water potential. Regarding irrigation scheduling, however, only sap flow and stem diameter variation measurements are needed for online simulation and daily model calibration. Model calibration is performed using a moving window of 4 days of past data of stem diameter variations. The research tool STACI (Software Tool for Automatic Control of Irrigation) was used to optimally combine the continuous measurements, the mathematical modelling and the real-time irrigation scheduling. The new methodology was successfully tested in a pilot-scale setup with young potted apple trees (Malus domestica Borkh) and its performance was critically evaluated