The role of digital health in supporting the achievement of the Sustainable Development Goals (SDGs)

PAHO, Dept Evidence & Intelligence Act Hlth, Washington, DC USAUniv Fed Sao Paulo, Sao Paulo, BrazilUniv Oberta Catalunya, Fac Hlth Sci, Barcelona, SpainUniv Fed Sao Paulo, Sao Paulo, BrazilWeb of Scienc

Stability analyses of sunflower (Helianthus annuus L.) hybrids for oleic acid and yield traits under multi location trials in Pakistan

The development of a hybrid with high oleic acid is an important breeding goal for sunflower. High oleic acid sunflower has better cooking quality due to low oxidation and rancidity. Hence, inbred lines differing for oleic acid content were selected, alongside the development of hybrids where one or both parents exhibited high oleic acid content in edible oil, and then evaluated at various sites (i.e. with comparatively low temperature during sunflower reproductive phase at Sargodha and Faisalabad; while high temperature and low humidity at Bhawalpur and Multan) in Pakistan during spring season. Moreover, autumn season was relatively cool and high humid for sites (Faisalabad and Sargodha, Pakistan). DNA profiling of hybrids differing for oleic acid content using N1-3F/N2-1R confirmed the presence of a high oleic acid allele in the hybrids. Oleic acid content and seed yield components were increased at high temperature and low humidity to a greater extent in spring than in autumn season. Among the hybrids, one (H5) had stable high oleic acid content during the spring season with higher seed yield and kernel to seed percentage than the check cultivars (Hysun-33 and FH-331). Analysis of the combining ability of two locations revealed a relationship between mean oleic acid contents and combining ability, thereby suggesting the effectiveness of selection in developing high oleic acid inbred lines. Newly developed inbred C.112.P was a positive combiner for oleic acid at all sites except Sargodha, while restorer populations such as RH.344, RH.345 and RH.347 were positive male combiners

Biosynthesis and characterization of bacterial cellulose membranes presenting relevant characteristics for air/gas filtration

Funding Information: This work was supported by the Associate Laboratory for Green Chemistry ─ LAQV, iNOVA4Health and the Associate Laboratory LS4FUTURE which are financed by Portuguese national funds from the Fundação para a Ciência e Tecnologia (FCT/MCTES, Portugal) ( UIDB/50006/2020 ; UIDP/50006/2020 ; UIDB/04462/2020 , UIDP/04462/2020 ; and LA/P/0087/2020 ; respectively). Arooj Fatima acknowledges FCT for PhD grant reference 2021.07557. BD. The authors acknowledge Professor Vítor D. Alves, from Instituto Superior de Agronomia, Universidade de Lisboa , for the support in the analysis of membrane mechanical properties. Funding Information: The surface area of bacterial cellulose membranes was determined using N2 adsorption/desorption isotherms and reported in Table 2. Both glucose and glycerol supported the bacterial growth that led to the production of membranes with diverse thicknesses, surface porosity and fiber diameter (Table 2). SEM images revealed that pores and fiber channels of varied sizes were present in the structure of all the bacterial cellulose membranes. Among all strains, FXV3 produced membranes with the highest surface area. Furthermore, and overall, adding ethanol made the bacterial cellulose membrane structure denser, resulting into low surface areas. Interestingly, NFXK3 showed the lowest surface area using both glucose and glycerol.This work was supported by the Associate Laboratory for Green Chemistry ─ LAQV, iNOVA4Health and the Associate Laboratory LS4FUTURE which are financed by Portuguese national funds from the Fundação para a Ciência e Tecnologia (FCT/MCTES, Portugal) (UIDB/50006/2020; UIDP/50006/2020; UIDB/04462/2020, UIDP/04462/2020; and LA/P/0087/2020; respectively). Arooj Fatima acknowledges FCT for PhD grant reference 2021.07557. BD. The authors acknowledge Professor Vítor D. Alves, from Instituto Superior de Agronomia, Universidade de Lisboa, for the support in the analysis of membrane mechanical properties. Publisher Copyright: © 2023The production of bacterial cellulose has gained prominence in recent years as an alternative for the sustainable production of materials that might be used in diverse processes and applications. The present study discusses the possibility of producing tailored bacterial cellulose membranes in situ, that present relevant characteristics for potential use in air/gas filtration. Various cultivation processes and characterization studies were performed to ascertain the suitability of Komagataeibacter sp. FXV3, Komagataeibacter sp. NFXK3, and K. intermedius LMG 18909 bacterial strains to produce cellulose membranes with diverse properties. Subsequently, the bacterial cellulose films produced were freeze-dried to obtain stable membranes, and extensively characterized for their physicochemical properties. The results obtained showed that different strains enabled the synthesis of membranes with distinctive morphological properties. Moreover, the different carbon sources and ethanol concentrations employed in the cultivation media led to modifications in the cellulose membranes produced by the different Komagataeibacter strains, which further impacted membrane morphology and, ultimately, gas filtration behavior. All the synthesized membranes were fully characterized, showing adequate mechanical properties, and tested for permeance of N2, CO2 and O2, opening perspectives for their use in air/gas filtration.publishersversionpublishe

Análisis del empoderamiento femenino en la narrativa cinematográfica de red de Pixar

El presente trabajo tuvo como objetivo general analizar el empoderamiento femenino en la narrativa cinematográfica de Red de Pixar. De forma que, la metodología consta de una investigación de enfoque cualitativo de tipo aplicada con un estudio de caso, el cual es el empoderamiento femenino. Es así como la muestra estuvo constituida por 30 escenas de dicha película que inciden en el empoderamiento de la mujer y aparecen los 11 personajes femeninos seleccionados. El tipo de muestreo fue por conveniencia, se usó la técnica de la observación y como instrumentos tres guías de observación para el estudio de cada una de las subcategorías (historia, imagen y sonido). Por último, como conclusión general de la investigación se puede decir que el empoderamiento femenino se manifiesta significativamente en la narrativa cinematográfica de Red de Pixar, pues, logró representar a las niñas y mujeres como personas independientes con total capacidad de tomar decisiones en su vida en los tres aspectos de dicha narrativa: historia, imagen y sonido

El inbound marketing para motivar la decisión de compra del consumidor de la Panadería Cotos, Piura 2022

La presente investigación tiene como objetivo, analizar la manera en que el inbound marketing puede motivar la decisión de compra del consumidor de la Panadería Cotos de Piura. Se utilizó la metodología aplicada, mixta, no experimental, transversal y descriptiva. En la población y muestreo se conformó por 380 clientes de la panadería, después en los instrumentos de recolección de datos se emplearon el cuestionario y la guía de entrevista, donde el cuestionario estuvo dirigido a la población conformada por hombres y mujeres de 18 a 65 años de edad que viven en el distrito de Piura y que pertenecen a los niveles socioeconómicos A, B y C, asimismo la guía de entrevista dirigida a la gerente general Mercedes Cotos. Los resultados demostraron que el 26.6% estimó que la panadería busca emplear distintas estrategias que le permitan tener mayor visibilidad en los buscadores web y el 11.3% afirma que su estado de ánimo influye al momento de adquirir algún producto de la panadería. Se llegó a la conclusión que, es fundamental potencializar las estrategias de inbound marketing, para poder crear el canal orgánico idóneo en captación de clientes, mejorar la visibilidad, generar mayor tráfico y potenciar las ventas con una mayor motivación en la decisión de compra del consumidor

Guía de anfibios y reptiles del complejo de humedales del Ypoá, Paraguay

El presente material fue elaborado en el marco del proyecto “Análisis de la herpetofauna asociada a los humedales del Complejo Ypoá”, cuyo objetivo fue caracterizar los anfibios y reptiles de estos humedales y difundir los resultados a fin de lograr una valoración social. Los datos presentados en esta guía fueron recabados en tres sitios, localizados en el distrito de Nueva Italia, Carapeguá y Quiindy (departamentos Central y Paraguarí, Paraguay). Las técnicas de muestreo consistieron en búsqueda activa, trampas pozo con cercos de conducción y trampas acuáticas de embudo, además de registros ocasionales aportados por pobladores de la zona. La riqueza registrada fue de 31 especies de anfibios y 22 especies de reptiles. Se presentan fotografías de estas especies, además de datos sobre la historia natural de las mismas. El área de estudio forma parte de la Reserva de Recursos Manejados Lago Ypoá y su zona de amortiguamiento y constituye un sitio RAMSAR. Por tanto, esta guía es un aporte al conocimiento de su herpetofauna.CONACYT - Consejo Nacional de Ciencia y TecnologíaPROCIENCI

CONSTANS–FKBP12 interaction contributes to modulation of photoperiodic flowering in Arabidopsis

Flowering time is a key process in plant development. Photoperiodic signals play a crucial role in the floral transition in Arabidopsis thaliana, and the protein CONSTANS (CO) has a central regulatory function that is tightly regulated at the transcriptional and post-translational levels. The stability of CO protein depends on a light-driven proteasome process that optimizes its accumulation in the evening to promote the production of the florigen FLOWERING LOCUS T (FT) and induce seasonal flowering. To further investigate the post-translational regulation of CO protein we have dissected its interactome network employing in vivo and in vitro assays and molecular genetics approaches. The immunophilin FKBP12 has been identified in Arabidopsis as a CO interactor that regulates its accumulation and activity. FKBP12 and CO interact through the CCT domain, affecting the stability and function of CO. fkbp12 insertion mutants show a delay in flowering time, while FKBP12 overexpression accelerates flowering, and these phenotypes can be directly related to a change in accumulation of FT protein. The interaction is conserved between the Chlamydomonas algal orthologs CrCO–CrFKBP12, revealing an ancient regulatory step in photoperiod regulation of plant development.Ministerio de Ciencia BIO2014-52425-P, BIO2017-83629-RJunta de Andalucía P08-AGR-03582, BIO-281European Union GA83831

REPRESENTAÇÕES SOCIAIS DE ALUNOS SOBRE CURSOS EAD: UMA ANÁLISE DOS SUJEITOS E DAS ESCOLHAS

Este trabalho pressupõe-se apresentar as Representações Sociais de alunos de cursos em EAD sobre sua escolha. Tem-se como objetivos conhecer as Representações Sociais de alunos matriculados em cursos de Ensino Superior - Licenciatura e Tecnológicos -, identificar o público que opta por um curso EAD e investigar os motivos das escolhas dos alunos. Trata-se de uma pesquisa à luz da Teoria das Representações Sociais, exploratória e com abordagem quanti-quali. Como instrumento de pesquisa utilizou-se questionário semiestruturado, aplicado a 181 alunos de cursos EAD, de uma Universidade do Vale do Paraíba. Como resultado obteve-se, nos dados sócios demográficos que, as mulheres são maioria nos cursos EAD e que os sujeitos que a compõe são pessoas que atuam no mercado de trabalho, tem família constituída e optam pela EAD por ser uma modalidade mais flexível em termos de tempo e lugar e ainda possibilitam a realização de um curso superior. Nota-se que as representações permeiam as escolhas dos cursos no que tange à escolha pessoal, a realização profissional e a profissionalização.

Glycated Hemoglobin and Subclinical Atherosclerosis in People Without Diabetes.

The metabolic injury caused by protein glycation, monitored as the level of glycated hemoglobin (HbA1c), is not represented in most risk scores (i.e., Systematic Coronary Risk Estimation or atherosclerotic cardiovascular disease risk scale). The purpose of this study was to assess the association between HbA1c and the extent of subclinical atherosclerosis (SA) and to better identify individuals at higher risk of extensive SA using HbA1c on top of key cardiovascular risk factors (CVRFs). A cohort of 3,973 middle-aged individuals from the PESA (Progression of Early Subclinical Atherosclerosis) study, with no history of cardiovascular disease and with HbA1c in the nondiabetic range, were assessed for the presence and extent of SA by 2-dimensional vascular ultrasound and noncontrast cardiac computed tomography. After adjusting for established CVRFs, HbA1c showed an association with the multiterritorial extent of SA (odds ratio: 1.05, 1.27, 1.27, 1.36, 1.80, 1.87, and 2.47 for HbA1c 4.9% to 5.0%, 5.1% to 5.2%, 5.3% to 5.4%, 5.5% to 5.6%, 5.7% to 5.8%, 5.9% to 6.0%, and 6.1% to 6.4%, respectively; reference HbA1c ≤4.8%; p < 0.001). The association was significant in all pre-diabetes groups and even below the pre-diabetes cut-off (HbA1c 5.5% to 5.6% odds ratio: 1.36 [95% confidence interval: 1.03 to 1.80]; p = 0.033). High HbA1c was associated with an increased risk of SA in low-risk individuals (p < 0.001), but not in moderate-risk individuals (p = 0.335). Relative risk estimations using Systematic Coronary Risk Estimation or atherosclerotic cardiovascular disease predictors confirmed that inclusion of HbA1c modified the risk of multiterritorial SA in most risk categories. Routine use of HbA1c can identify asymptomatic individuals at higher risk of SA on top of traditional CVRFs. Lifestyle interventions and novel antidiabetic medications might be considered to reduce both HbA1c levels and SA in individuals without diabetes.The PESA study is funded by the Centro Nacional de Investigaciones Cardiovasculares (CNIC) and Banco Santander. The study also receives funding from the Instituto de Salud Carlos III (ISCIII, PI15/ 02019, PI17/00590, and PI20/00819) and the European Regional Development Fund (ERDF) “A way to make Europe.” The CNIC is supported by the ISCIII, the Ministerio de Ciencia e Innovación, and the Pro CNIC Foundation. Dr. Bueno has received grants from the Instituto de Salud Carlos III; has received consultancy fees from Bayer, Novartis, Ferrer, MEDSCAPE-the Heart-org, and Janssen; has received grants, consultancy fees, and nonfinancial support from AstraZeneca; and has received grants and consultancy fees from Bristol Myers Squibb-Pfizer, all unrelated to the present study. Dr. Ibanez is supported by the European Commission (ERC-CoG grant No 819775), the Spanish Ministry of Science and Innovation (MCN, ‘RETOS 2019’ grant No PID2019-107332RB-I00), and the Comunidad de Madrid (S2017/BMD-3867 RENIM-CM). The funders had no role in the design and conduct of the study; the collection, management, analysis, and interpretation of the data; the preparation, review, or approval of the manuscript; or the decision to submit the manuscript for publication. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose.S

Loss of SRSF3 in Cardiomyocytes Leads to Decapping of Contraction-Related mRNAs and Severe Systolic Dysfunction

RATIONALE: RBPs (RNA binding proteins) play critical roles in the cell by regulating mRNA transport, splicing, editing, and stability. The RBP SRSF3 (serine/arginine-rich splicing factor 3) is essential for blastocyst formation and for proper liver development and function. However, its role in the heart has not been explored. OBJECTIVE: To investigate the role of SRSF3 in cardiac function. METHODS AND RESULTS: Cardiac SRSF3 expression was high at mid gestation and decreased during late embryonic development. Mice lacking SRSF3 in the embryonic heart showed impaired cardiomyocyte proliferation and died in utero. In the adult heart, SRSF3 expression was reduced after myocardial infarction, suggesting a possible role in cardiac homeostasis. To determine the role of this RBP in the adult heart, we used an inducible, cardiomyocyte-specific SRSF3 knockout mouse model. After SRSF3 depletion in cardiomyocytes, mice developed severe systolic dysfunction that resulted in death within 8 days. RNA-Seq analysis revealed downregulation of mRNAs encoding sarcomeric and calcium handling proteins. Cardiomyocyte-specific SRSF3 knockout mice also showed evidence of alternative splicing of mTOR (mammalian target of rapamycin) mRNA, generating a shorter protein isoform lacking catalytic activity. This was associated with decreased phosphorylation of 4E-BP1 (eIF4E-binding protein 1), a protein that binds to eIF4E (eukaryotic translation initiation factor 4E) and prevents mRNA decapping. Consequently, we found increased decapping of mRNAs encoding proteins involved in cardiac contraction. Decapping was partially reversed by mTOR activation. CONCLUSIONS: We show that cardiomyocyte-specific loss of SRSF3 expression results in decapping of critical mRNAs involved in cardiac contraction. The molecular mechanism underlying this effect likely involves the generation of a short mTOR isoform by alternative splicing, resulting in reduced 4E-BP1 phosphorylation. The identification of mRNA decapping as a mechanism of systolic heart failure may open the way to the development of urgently needed therapeutic tools.This study was supported by grants from the European Union (CardioNeT-ITN-289600 and CardioNext-ITN-608027 to E.L-P.), from the Spanish Ministerio de Economía y Competitividad (RTI2018-096961-BI00, SAF2015-65722-R and SAF2012-31451 to E.L-P.; BIO2015-67580-P and PGC2018-097019-B-I00 to J.V.), the Spanish Carlos III Institute of Health (CPII14/00027 to E.L-P, RD12/0042/066 to P.G.-P. and E.L-P, and RD12/0042/0056, PRB2-IPT13/0001-ISCIII-SGEFI/FEDER, ProteoRed to J.V.), the Madrid Regional Government (2010-BMD-2321 “Fibroteam” to E.L-P.). This study was also supported by the Plan Estatal de I+D+I 2013-2016 – European Regional Development Fund (ERDF) “A way of making Europe”, Spain. The CNIC is supported by the Ministerio de Ciencia, Innovación y Universidades (MCNU) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505).S