2,609 research outputs found
A dual-mode mobile phone microscope using the onboard camera flash and ambient light
Mobile phone microscopes are a natural platform for point-of-care imaging, but current solutions require an externally powered illumination source, thereby adding bulk and cost. We present a mobile phone microscope that uses the internal flash or sunlight as the illumination source, thereby reducing complexity whilst maintaining functionality and performance. The microscope is capable of both brightfield and darkfield imaging modes, enabling microscopic visualisation of samples ranging from plant to mammalian cells. We describe the microscope design principles, assembly process, and demonstrate its imaging capabilities through the visualisation of unlabelled cell nuclei to observing the motility of cattle sperm and zooplankton.A. Orth, E. R Wilson, J. G. Thompson and B. C. Gibso
Chromosomal control of non-gliadin proteins from the 70% ethanol extract of wheat endosperm
The non-gliadin fraction of the 70% ethanol extracts of compensated nulli-tetrasomics and ditelosomics of Triticum aestivum cv. Chinese Spring has been analyzed by combined electrofocusing and electrophoresis. Seventeen of the 21 protein map components of the euploid have been ascribed to eight chromosomes: 4A, 3BS, 6BS, 7BS, 3D, 4D, 5D and 7DS.
The relationship of the different map components with other proteins previously associated with the same chromosomes is discusse
Pion radii in nonlocal chiral quark model
The electromagnetic radius of the charged pion and the transition radius of
the neutral pion are calculated in the framework of the nonlocal chiral quark
model. It is shown in this model that the contributions of vector mesons to the
pion radii are noticeably suppressed in comparison with a similar contribution
in the local Nambu--Jona-Lasinio model. The form-factor for the process
gamma*pi+pi- is calculated for the -1 GeV^2<q^2<1.6 GeV^2. Our results are in
satisfactory agreement with experimental data.Comment: 7 pages, 7 figure
Fluorescence brightness and photostability of individual copper (I) oxide nanocubes
Conventional organic fluorophores lose their ability to fluoresce after repeated exposure to excitation light due to photobleaching. Therefore, research into emerging bright and photostable nanomaterials has become of great interest for a range of applications such as bio-imaging and tracking. Among these emerging fluorophores, metal oxide-based nanomaterials have attracted significant attention as a potential multifunctional material with photocatalytic and angeogenisis abilities in addition to fluorescnce applications. However, most of these applications are highly dependent on size, morphology, and chemo-physical properties of individual particles. In this manuscript, we present a method to study the intrinsic optical characteristics of individual copper (I) oxide (Cu2O) nanocubes. When excited at 520ânm using only 11â”W excitation power (1.7âW/cm2), individual nanocubes were observed to emit light with peak wavelengths ~760ânm which is conveniently within the near-infrared 1 (NIR1) biological window where tissue autofluorescence is minimal. Bright and photostable fluorescence was observed with intensities up to 487âK counts/s under constant illumination for at least 2âminutes with a brightness approximately four times higher than the autofluorescence from a fixed cumulus-oocyte complex. With near-IR emission, high fluorescence brightness, and outstanding photostability, Cu2O nanocubes are attractive candidates for long-term fluorescent bioimaging applications.Nafisa Zohora, Ahmad Esmaielzadeh Kandjani, Antony Orth, Hannah M. Brown, Mark R. Hutchinson and Brant C. Gibso
Thermal and Chemical Freeze-out in Spectator Fragmentation
Isotope temperatures from double ratios of hydrogen, helium, lithium,
beryllium, and carbon isotopic yields, and excited-state temperatures from
yield ratios of particle-unstable resonances in 4He, 5Li, and 8Be, were
determined for spectator fragmentation, following collisions of 197Au with
targets ranging from C to Au at incident energies of 600 and 1000 MeV per
nucleon. A deviation of the isotopic from the excited-state temperatures is
observed which coincides with the transition from residue formation to
multi-fragment production, suggesting a chemical freeze-out prior to thermal
freeze-out in bulk disintegrations.Comment: 14 pages, 10 figures, submitted to Phys. Rev. C, small changes as
suggested by the editors and referee
Contrasting drought legacy effects on gross primary productivity in a mixed versus pure beech forest
Droughts affect terrestrial ecosystems directly and concurrently and can additionally induce lagged effects in subsequent seasons and years. Such legacy effects of drought on vegetation growth and state have been widely studied in tree ring records and satellite-based vegetation greenness, while legacies on ecosystem carbon fluxes are still poorly quantified and understood. Here, we focus on two ecosystem monitoring sites in central Germany with a similar climate but characterised by different species and age structures. Using eddy covariance measurements, we detect legacies on gross primary productivity (GPP) by calculating the difference between random forest model estimates of potential GPP and observed GPP. Our results showed that, at both sites, droughts caused significant legacy effects on GPP at seasonal and annual timescales, which were partly explained by reduced leaf development. The GPP reduction due to drought legacy effects is of comparable magnitude to the concurrent drought effects but differed between two neighbouring forests with divergent species and age structures. The methodology proposed here allows the quantification of the temporal dynamics of legacy effects at the sub-seasonal scale and the separation of legacy effects from model uncertainties. The application of the methodology at a larger range of sites will help us to quantify whether the identified lag effects are general and on which factors they may depend.ISSN:1726-4170ISSN:1726-417
Multiplicity correlations of intermediate-mass fragments with pions and fast protons in 12C + 197Au
Low-energy pi+ (E < 35 MeV) from 12C+197Au collisions at incident energies
from 300 to 1800 MeV per nucleon were detected with the Si-Si(Li)-CsI(Tl)
calibration telescopes of the INDRA multidetector. The inclusive angular
distributions are approximately isotropic, consistent with multiple
rescattering in the target spectator. The multiplicity correlations of the
low-energy pions and of energetic protons (E > 150 MeV) with intermediate-mass
fragments were determined from the measured coincidence data. The deduced
correlation functions 1 + R \approx 1.3 for inclusive event samples reflect the
strong correlations evident from the common impact-parameter dependence of the
considered multiplicities. For narrow impact-parameter bins (based on
charged-particle multiplicity), the correlation functions are close to unity
and do not indicate strong additional correlations. Only for pions at high
particle multiplicities (central collisions) a weak anticorrelation is
observed, probably due to a limited competition between these emissions.
Overall, the results are consistent with the equilibrium assumption made in
statistical multifragmentation scenarios. Predictions obtained with
intranuclear cascade models coupled to the Statistical Multifragmentation Model
are in good agreement with the experimental data.Comment: 9 pages, 11 figures, subm. to EPJ
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Clean option: Berkeley Pit water treatment and resource recovery strategy
The US Department of Energy (DOE), Office of Technology Development, established the Resource Recovery Project (RRP) in 1992 as a five-year effort to evaluate and demonstrate multiple technologies for recovering water, metals, and other industrial resources from contaminated surface and groundwater. Natural water resources located throughout the DOE complex and the and western states have been rendered unusable because of contamination from heavy metals. The Berkeley Pit, a large, inactive, open pit copper mine located in Butte, Montana, along with its associated groundwater system, has been selected by the RRP for use as a feedstock for a test bed facility located there. The test bed facility provides the infrastructure needed to evaluate promising technologies at the pilot plant scale. Data obtained from testing these technologies was used to assess their applicability for similar mine drainage water applications throughout the western states and at DOE. The objective of the Clean Option project is to develop strategies that provides a comprehensive and integrated approach to resource recovery using the Berkeley Pit water as a feedstock. The strategies not only consider the immediate problem of resource recovery from the contaminated water, but also manage the subsequent treatment of all resulting process streams. The strategies also employ the philosophy of waste minimization to optimize reduction of the waste volume requiring disposal, and the recovery and reuse of processing materials
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