Comparison of the accumulation of astaxanthin in Haematococcus pluvialis and other green microalgae under N-starvation and high light conditions

Haematococcus pluvialis gave the highest astaxanthin accumulation rate (2.7 mg l−1 day−1) and total astaxanthin content ( 22.7 mg g−1 biomass). Astaxanthin accumulation in Neochloris wimmeri, Protosiphon botryoides, Scotiellopsis oocystiformis, Chorella zofingiensis and Scenedesmus vacuolatus was, respectively, 19.2, 14.3, 10.9, 6.8 and 2.7 mg astaxanthin g−1 biomass, respectively.Xunta de Galicia; PGIDT00PXI10301PR

Hydrology influences carbon flux through metabolic pathways in the hypolimnion of a Mediterranean reservoir

Global change is modifying meteorological and hydrological factors that influence the thermal regime of water bodies. These modifications can lead to longer stratification periods with enlarged hypolimnetic anoxic periods, which can promote heterotrophic anaerobic processes and alter reservoir carbon cycling. Here, we quantified aerobic and anaerobic heterotrophic processes (aerobic respiration, denitrification, iron and manganese reduction, sulfate reduction, and methanogenesis) on dissolved inorganic carbon (DIC) production in the hypolimnion of a Mediterranean reservoir (El Gergal, Spain) under two contrasting hydrological conditions: a wet year with heavy direct rainfall and frequent water inputs from upstream reservoirs, and a dry year with scarce rainfall and negligible water inputs. During the wet year, water inputs and rainfall induced low water column thermal stability and earlier turnover. By contrast, thermal stratification was longer and more stable during the dry year. During wet conditions, we observed lower DIC accumulation in the hypolimnion, mainly due to weaker sulfate reduction and methanogenesis. By contrast, longer stratification during the dry year promoted higher hypolimnetic DIC accumulation, resulting from enhanced methanogenesis and sulfate reduction, thus increasing methane emissions and impairing reservoir water quality. Aerobic respiration, denitrification and metal reduction produced a similar amount of DIC in the hypolimnion during the two studied years. All in all, biological and geochemical (calcite dissolution) processes explained most of hypolimnetic DIC accumulation during stratification regardless of the hydrological conditions, but there is still ~ 30% of hypolimnetic DIC production that cannot be explained by the processes contemplated in this study and the assumptions made.This research was funded by project Alter-C (PID2020-114024GB-C31, PID2020-114024GB-C32, PID2020-114024GB-C33) of Spanish Ministry of Science and Innovation (Spanish Research Agency, AEI). JJM-P was supported by a Spanish FPI grant (RE2018-083596). EMASESA staff provide essential technical support during field surveys. R.M. acknowledges funding from Generalitat de Catalunya through the Consolidated Research Group 2017SGR1124 and the CERCA programme. Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Funding for open access charge: Universidad de Málaga / CBUA

Diapiric growth within an Early Jurassic rift basin: The Tazoult salt wall (central High Atlas, Morocco)

The central High Atlas (Morocco) constitutes a diapiric province that hosts a complex array of elongated diapirs and minibasins that formed during the Lower Jurassic rift of the Atlas Basin. This paper aims to study the structure and growth evolution of the Tazoult diapiric wall, located in the central High Atlas, by means of structural and sedimentological fieldwork integrated with remote sensing mapping. The Tazoult salt wall is a 20km long×3km wide NE-SW trending ridge that exposes Upper Triassic red beds and basalts along its core. The succession flanking the salt wall ranges from Hettangian to Bajocian ages displaying spectacular sedimentary wedges in the SE and NW flanks. The Hettangian-early Sinemurian carbonates mainly crop out as blocks embedded in the core rocks. The ~1km thick Pliensbachian platform carbonates display large subvertical flap structures along the flanks of the Tazoult salt wall with unconformities bounding tapered composite halokinetic sequences. In contrast, the ~2.5km thick late Pliensbachian-Aalenian mixed deposits form tabular composite halokinetic sequences displaying small-scale hook halokinetic sequences. Passive diapirism resulted in the lateral extrusion of the evaporite-bearing rocks to form an allochthonous salt sheet toward the adjacent SE Amezraï minibasin. The Bajocian platform carbonates partially fossilized the Tazoult salt wall and thus constitute a key horizon to constrain the timing of diapir growth and discriminate diapirism from Alpine shortening. The Pliensbachian carbonate platform evolved as a long flap structure during the early growth of the Tazoult salt wall, well before the onset of the Alpine shortening. © 2016. American Geophysical Union. All Rights Reserved.Additional funding was provided by the Spanish Ministry of Education and Science (MEC) through the projects Intramural Especial (CSIC 201330E030) and 201530E082), Atiza (CGL2009-1355), Tecla (CGL2011-26670), and the postdoctoral research contract to E.S. (CSIC-FSE 2007-2013 JAE-Doc), as well as by the Generalitat de Catalunya (2014GSR251).Peer reviewe

Fuente de Alimentación para los Imanes Superconductores del Acelerador de Partículas Europeo XFEL

En este artículo se presenta la fuente de alimentación que se está diseñando para alimentar los imanes superconductores del acelerador de partículas europeo XFEL que se está construyendo en Hamburgo, cuyas características le hacen el más avanzado del mundo. Un imán superconductor es una carga muy inductiva que debe ser controlada en corriente y que presenta una caída de tensión muy baja cuando está en modo superconductor. La fuente debe ser capaz de alimentar esta carga con una alta fiabilidad e incorporar varias protecciones que protejan esta carga tan especial

Relationship of problematic cannabis use among youth in Spain with perceived risk, environmental factors and sociodemographic factors

This is the final version. Available on open access from Socidrogalcohol via the DOI in this record. The relationship of problematic cannabis consumption with perceived risk, socioenvironmental and sociodemographic factors among youth in Spain is not well known. The aims of this study are: 1) to describe the patterns of cannabis consumption (problematic and non-problematic) in Spanish youth, and 2) to explore whether problematic cannabis consumption is related to perceived risk, environmental factors and individual sociodemographic characteristics. A cross-sectional design based on data from the 2015/16 Spanish Household Survey on Alcohol and Drugs (EDADES) was performed. Individuals between 15 and 35 years old having used cannabis during the last year with a complete Cannabis Abuse Screening Test (CAST) were included (N = 1,674). Problematic consumption (CAST >= 7) was considered as dependent variable. Perceived risk, environmental factors (availability of the substance and exposure to consumption situations) and sociodemographic factors were taken as independent variables. Descriptive analyses of consumption patterns were performed and univariable and multivariable Poisson regression models were done. All analyses were stratified by gender. Problematic cannabis consumption was more frequent among men (38.9 %) than among women (23.2 %). While among men, problematic use was related to environmental factors and educational level, among women it was associated with perceived risk and unemployment. Problematic cannabis consumption among Spanish youth is associated with different types of gender-related factors. Due to its representativeness at the population level and the validity of the measures, these results might have important implications on the development of prevention strategies targeted at problematic cannabis consumptionCIBER Epidemiology and Public Health (CIBERESP

The impact of mindfulness on well-being and performance in the workplace: an inclusive systematic review of the empirical literature

Work can be demanding, imposing challenges that can be detrimental to the physical and mental health of workers. Efforts are therefore underway to develop practices and initiatives that may improve occupational well-being. These include interventions based on mindfulness meditation. This paper offers a systematic review of empirical studies featuring analyses of mindfulness in occupational contexts. Databases were reviewed from the start of records to January 2016. Eligibility criteria included experimental and correlative studies of mindfulness conducted in work settings, with a variety of well-being and performance measures. A total of 153 papers met the eligibility criteria and were included in the systematic review, comprising 12,571 participants. Mindfulness was generally associated with positive outcomes in relation to most measures. However, the quality of the studies was inconsistent, so further research is needed, particularly involving high-quality randomized control trials

Mindfulness-based interventions in the workplace: An inclusive systematic review and meta-analysis of their impact upon wellbeing

Given the demanding nature of many professions, efforts are ongoing to develop initiatives to improve occupational wellbeing, including mindfulness-based interventions (MBIs). To assess the efficacy of MBIs, meta-analytic procedures were conducted on 35 randomized controlled trials derived from an earlier inclusive systematic literature search (covering all occupations, MBIs, and wellbeing-related outcomes). Mindfulness had significant moderate effects on deficit-based outcomes such as stress (SMD = −0.57), anxiety (SMD = −0.57), distress (SMD = −0.56), depression (SMD = −0.48), and burnout (SMD = −0.36), and significant moderate to small effects on asset-based outcomes like health (SMD = 0.63), job performance (SMD = 0.43), compassion and empathy (SMD = 0.42), mindfulness (SMD = 0.39), and positive wellbeing (SMD = 0.36), while no significant effects were observed for depression or emotional regulation. However, the quality of the studies was inconsistent, suggesting more high-quality randomised controlled trials are needed

SARS-CoV-2 Omicron BA.1 variant breakthrough infections in nursing home residents after an homologous third dose of the Comirnaty® COVID-19 vaccine: Looking for correlates of protection

8 páginas, 2 figuras. Texto completo en PubMedCentral: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348298/pdf/JMV-94-4216.pdfWe investigated whether peripheral blood levels of SARS-CoV-2 Spike (S) receptor binding domain antibodies (anti-RBD), neutralizing antibodies (NtAb) targeting Omicron S, and S-reactive-interferon (IFN)-γ-producing CD4+ and CD8+ T cells measured after a homologous booster dose (3D) with the Comirnaty® vaccine was associated with the likelihood of subsequent breakthrough infections due to the Omicron variant. An observational study including 146 nursing home residents (median age, 80 years; range, 66-99; 109 female) evaluated for an immunological response after 3D (at a median of 16 days). Anti-RBD total antibodies were measured by chemiluminescent immunoassay. NtAb were quantified by an Omicron S pseudotyped virus neutralization assay. SARS-CoV-2-S specific-IFNγ-producing CD4+ and CD8+ T cells were enumerated by whole-blood flow cytometry for intracellular cytokine staining. In total, 33/146 participants contracted breakthrough Omicron infection (symptomatic in 30/33) within 4 months after 3D. Anti-RBD antibody levels were comparable in infected and uninfected participants (21 123 vs. 24 723 BAU/ml; p = 0.34). Likewise, NtAb titers (reciprocal IC50 titer, 157 vs. 95; p = 0.32) and frequency of virus-reactive CD4+ (p = 0.82) and CD8+ (p = 0.91) T cells were similar across participants in both groups. anti-RBD antibody levels and NtAb titers estimated at around the time of infection were also comparable (3445 vs. 4345 BAU/ml; p = 0.59 and 188.5 vs. 88.9; p = 0.70, respectively). Having detectable NtAb against Omicron or SARS-CoV-2-S-reactive-IFNγ-producing CD4+ or CD8+ T cells after 3D was not correlated with increased protection from breakthrough infection (OR, 1.50; p = 0.54; OR, 0.0; p = 0.99 and OR 3.70; p = 0.23, respectively). None of the immune parameters evaluated herein, including NtAb titers against the Omicron variant, may reliably predict at the individual level the risk of contracting COVID-19 due to the Omicron variant in nursing home residents.Ignacio Torres (Río Hortega Contract; CM20/00090), Estela Giménez (Juan Rodés Contract, JR18/00053), and Eliseo Albert (Juan Rodés Contract; JR20/00011) hold contracts funded by the Carlos III Health Institute (cofinanced by the European Regional Development Fund, ERDF/FEDER). Ron Geller holds a Ramon y Cajal fellowship from the Spanish Ministerio de Economía y Competitividad (RYC‐2015‐17517). This study work was supported by Instituto de Salud Carlos III, Madrid, Spain (FIS, PI21/00563) to David Navarro, and by the European Commission NextGenerationEU fund (EU 2020/2094), through CSIC's Global Health Platform (PTI Salud Global) to Ron Geller.Peer reviewe

Differential clinical characteristics and prognosis of intraventricular conduction defects in patients with chronic heart failure

Intraventricular conduction defects (IVCDs) can impair prognosis of heart failure (HF), but their specific impact is not well established. This study aimed to analyse the clinical profile and outcomes of HF patients with LBBB, right bundle branch block (RBBB), left anterior fascicular block (LAFB), and no IVCDs. Clinical variables and outcomes after a median follow-up of 21 months were analysed in 1762 patients with chronic HF and LBBB (n = 532), RBBB (n = 134), LAFB (n = 154), and no IVCDs (n = 942). LBBB was associated with more marked LV dilation, depressed LVEF, and mitral valve regurgitation. Patients with RBBB presented overt signs of congestive HF and depressed right ventricular motion. The LAFB group presented intermediate clinical characteristics, and patients with no IVCDs were more often women with less enlarged left ventricles and less depressed LVEF. Death occurred in 332 patients (interannual mortality = 10.8%): cardiovascular in 257, extravascular in 61, and of unknown origin in 14 patients. Cardiac death occurred in 230 (pump failure in 171 and sudden death in 59). An adjusted Cox model showed higher risk of cardiac death and pump failure death in the LBBB and RBBB than in the LAFB and the no IVCD groups. LBBB and RBBB are associated with different clinical profiles and both are independent predictors of increased risk of cardiac death in patients with HF. A more favourable prognosis was observed in patients with LAFB and in those free of IVCDs. Further research in HF patients with RBBB is warranted