First report of caprine abortions due to Chlamydia abortus in Argentina.

Infectious abortions of goats in Argentina are mainly associated with brucellosis and toxoplasmosis. In this paper, we describe an abortion outbreak in goats caused by Chlamydia abortus. Seventy out of 400 goats aborted. Placental smears stained with modified Ziehl-Neelsen stain showed many chlamydia-like bodies within trophoblasts. One stillborn fetus was necropsied and the placenta was examined. No gross lesions were seen in the fetus, but the inter-cotyledonary areas of the placenta were thickened and covered by fibrino-suppurative exudate. The most consistent microscopic finding was found in the placenta and consisted of fibrinoid necrotic vasculitis, with mixed inflammatory infiltration in the tunica media. Immunohistochemistry of the placenta was positive for Chlamydia spp. The results of polymerase chain reaction targeting 23S rRNA gene performed on placenta were positive for Chlamydia spp. An analysis of 417 amplified nucleotide sequences revealed 99% identity to those of C. abortus pm225 (GenBank AJ005617) and pm112 (GenBank AJ005613) isolates. To the best of our knowledge, this is the first report of abortion associated with C. abortus in Argentina

First report of caprine abortions due to <i>Chlamydia abortus</i> in Argentina

Infectious abortions of goats in Argentina are mainly associated with brucellosis and toxoplasmosis. In this paper, we describe an abortion outbreak in goats caused by Chlamydia abortus. Seventy out of 400 goats aborted. Placental smears stained with modified Ziehl-Neelsen stain showed many chlamydia-like bodies within trophoblasts. One stillborn fetus was necropsied and the placenta was examined. No gross lesions were seen in the fetus, but the inter-cotyledonary areas of the placenta were thickened and covered by fibrino-suppurative exudate. The most consistent microscopic finding was found in the placenta and consisted of fibrinoid necrotic vasculitis, with mixed inflammatory infiltration in the tunica media. Immunohistochemistry of the placenta was positive for Chlamydia spp. The results of polymerase chain reaction targeting 23S rRNA gene performed on placenta were positive for Chlamydia spp. An analysis of 417 amplified nucleotide sequences revealed 99% identity to those of C. abortus pm225 (GenBank AJ005617) and pm112 (GenBank AJ005613) isolates. To the best of our knowledge, this is the first report of abortion associated with C. abortus in Argentina.Facultad de Ciencias VeterinariasCentro de Diagnóstico e Investigaciones Veterinaria

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Salmonella enterica enterica y Salmonella enterica diarizonae aisladas de ofidios en el Parque Zoológico de La Plata, Argentina

Fil: Vigo, Germán B. Cátedra de Microbiología; Argentina.Fil: Caffer, María Inés. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Enterobacterias; Argentina.Fil: Origlia, Javier A. Cátedra de Patología Aviar y Pilíferos; Argentina.Fil: Carriquiriborde, M. Cátedra de Animales de Laboratorio; Argentina.Fil: Leotta, G. A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.La salmonelosis es reconocida por ser una de las más importantes causas de problemas de Salud Pública a nivel mundial. En el presente estudio, Salmonella fue aislada de 12 de 30 (40%) muestras de hisopados cloacales de víboras. Se encontraron un total de ocho diferentes serovares de Salmonella. Los aislamientos correspondieron al género Salmonella, especie enterica, subespecie enterica (42%, Salmonella Newport, Saintpaul y Carrau) y Salmonella enterica diarizonae (58%, IIIb 17:-:-, IIIb 48:i:z. IIIb 38:z:- y IIIb 65:k:z). Todos los aislamientos fueron susceptibles a los antimicrobianos utilizados: ampicilina, cefalotina, cloranfenicol, entamicina, estreptomicina, sulfametoxazol-trimetoprima, tetraciclina, ciprofloxacina, norfloxacina, nitrofurantoína, fosfomicina y polimixina. Las víboras pueden ser una fuente de salmonelosis para los humanos. Existen pocos estudios sobre Salmonella realizados en víboras en parques zoológicos en Latinoamérica. Este es el primer informe sobre este tipo de relevamiento realizado en víboras de zoológico en la República Argentina

Salmonella enterica enterica y Salmonella enterica diarizonae aisladas de ofidios en el Parque Zoológico de La Plata, Argentina

Fil: Vigo, Germán B. Cátedra de Microbiología; Argentina.Fil: Caffer, María Inés. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Enterobacterias; Argentina.Fil: Origlia, Javier A. Cátedra de Patología Aviar y Pilíferos; Argentina.Fil: Carriquiriborde, M. Cátedra de Animales de Laboratorio; Argentina.Fil: Leotta, G. A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.La salmonelosis es reconocida por ser una de las más importantes causas de problemas de Salud Pública a nivel mundial. En el presente estudio, Salmonella fue aislada de 12 de 30 (40%) muestras de hisopados cloacales de víboras. Se encontraron un total de ocho diferentes serovares de Salmonella. Los aislamientos correspondieron al género Salmonella, especie enterica, subespecie enterica (42%, Salmonella Newport, Saintpaul y Carrau) y Salmonella enterica diarizonae (58%, IIIb 17:-:-, IIIb 48:i:z. IIIb 38:z:- y IIIb 65:k:z). Todos los aislamientos fueron susceptibles a los antimicrobianos utilizados: ampicilina, cefalotina, cloranfenicol, entamicina, estreptomicina, sulfametoxazol-trimetoprima, tetraciclina, ciprofloxacina, norfloxacina, nitrofurantoína, fosfomicina y polimixina. Las víboras pueden ser una fuente de salmonelosis para los humanos. Existen pocos estudios sobre Salmonella realizados en víboras en parques zoológicos en Latinoamérica. Este es el primer informe sobre este tipo de relevamiento realizado en víboras de zoológico en la República Argentina

Detection and molecular characterization of Chlamydia psittaci and Chlamydia abortus in psittacine pet birds in Buenos Aires province, Argentina

Fil: Origlia, Javier Anibal. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología de Aves y Pilíferos; ArgentinaFil: Cadario, Maria E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina.Fil: Frutos, María Celia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina.Fil: Piscopo, Miguel Víctor. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología de Aves y Pilíferos; Argentina.Fil: Frutos, María Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba; Argentina.Fil: Cuffini, Cecilia Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina.Fil: Cuffini, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba; Argentina.Fil: Petruccelli, Miguel Ángel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología de Aves y Pilíferos; Argentina.In order to determine the presence and genetic diversity of Chlamydia spp. in thenorth-eastern area of Buenos Aires province, Argentina, conjunctival, oropharyngeal, cloacalswab and tissues were collected from a total of 90 psittacine pet birds of different age and clinicalmanifestations. Through molecular methods, Chlamydiaceae was detected in 30% (27/90)of the samples, out of which 70.3% (19/27) were positive for Chlamydia psittaci and 14.9%(4/27) for Chlamydia abortus. Nine C. psittaci positive samples were genotyped by ompA genesequences, 8 clustered within genotype A and 1 within genotype B. A significant associationwas observed between the presence of Chlamydia spp. and the manifestation of clinical signscompatible with chlamydiosis, as well as with the age of the birds (younger than one year old).This report contributes to the improvement of our understanding of chlamydial agents in our countryhttp://www.elsevier.es/es-revista-revista-argentina-microbiologia-372-avanceinfo:eu-repo/semantics/publishedVersionFil: Origlia, Javier Anibal. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología de Aves y Pilíferos; ArgentinaFil: Cadario, Maria E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina.Fil: Frutos, María Celia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina.Fil: Piscopo, Miguel Víctor. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología de Aves y Pilíferos; Argentina.Fil: Frutos, María Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba; Argentina.Fil: Cuffini, Cecilia Gabriela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina.Fil: Cuffini, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba; Argentina.Fil: Petruccelli, Miguel Ángel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología de Aves y Pilíferos; Argentina.Enfermedades Infecciosa

HARMONI: a single-field wide-band integral-field spectrograph for the European ELT

Trabajo presentado en SPIE Astronomical Telescopes, celebrado en San Diego (California), del 15 de junio al 2 de julio de 2010We describe the results of a Phase A study for a single field, wide band, near-infrared integral field spectrograph for the European Extremely Large Telescope (E-ELT). HARMONI, the High Angular Resolution Monolithic Optical & Nearinfrared Integral field spectrograph, provides the E-ELT’s core spectroscopic requirement. It is a work-horse instrument, with four different spatial scales, ranging from seeing to diffraction-limited, and spectral resolving powers of 4000, 10000 & 20000 covering the 0.47 to 2.45 μm wavelength range. It is optimally suited to carry out a wide range of observing programs, focusing on detailed, spatially resolved studies of extended objects to unravel their morphology, kinematics and chemical composition, whilst also enabling ultra-sensitive observations of point sources. We present a synopsis of the key science cases motivating the instrument, the top level specifications, a description of the opto-mechanical concept, operation and calibration plan, and image quality and throughput budgets. Issues of expected performance, complementarity and synergies, as well as simulated observations are presented elsewhere in these proceeding

ANDES, the high resolution spectrograph for the ELT: science goals, project overview, and future developments

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