STORYTELLING COMO ESTRATÉGIA PEDAGÓGICA PARA EDUCAÇÃO JURÍDICA

A pesquisa investiga o storytelling como forma de abordar um dado componente curricular em aula expositiva no curso de graduação em Direito. No ensino jurídico, há obrigatoriedade de uso de metodologias ativas com a vigência da Resolução n. 5, 17 de dezembro de 2018, do Conselho Nacional de Educação. A investigação, nesse contexto, parte da possibilidade de utilizar o storytelling, arte de contar histórias, como forma de transmissão dos componentes curriculares. O percurso estudado concentra-se em revisão da literatura, tendo, por conclusão, que no ensino do direito, a narração de histórias é uma ferramenta importante para o processo de ensino-aprendizagem

Validation of a physical anthropology methodology using mandibles for gender estimation in a Brazilian population

Validation studies of physical anthropology methods in the different population groups are extremely important, especially in cases in which the population variations may cause problems in the identification of a native individual by the application of norms developed for different communities. OBJECTIVE: This study aimed to estimate the gender of skeletons by application of the method of Oliveira, et al. (1995), previously used in a population sample from Northeast Brazil. MATERIAL AND METHODS: The accuracy of this method was assessed for a population from Southeast Brazil and validated by statistical tests. The method used two mandibular measurements, namely the bigonial distance and the mandibular ramus height. The sample was composed of 66 skulls and the method was applied by two examiners. The results were statistically analyzed by the paired t test, logistic discriminant analysis and logistic regression. RESULTS: The results demonstrated that the application of the method of Oliveira, et al. (1995) in this population achieved very different outcomes between genders, with 100% for females and only 11% for males, which may be explained by ethnic differences. However, statistical adjustment of measurement data for the population analyzed allowed accuracy of 76.47% for males and 78.13% for females, with the creation of a new discriminant formula. CONCLUSION: It was concluded that methods involving physical anthropology present high rate of accuracy for human identification, easy application, low cost and simplicity; however, the methodologies must be validated for the different populations due to differences in ethnic patterns, which are directly related to the phenotypic aspects. In this specific case, the method of Oliveira, et al. (1995) presented good accuracy and may be used for gender estimation in Brazil in two geographic regions, namely Northeast and Southeast; however, for other regions of the country (North, Central West and South), previous methodological adjustment is recommended as demonstrated in this study

Do Circadian Preferences Influence the Sleep Patterns of Night Shift Drivers?

Objective: the objective of this study was to analyze the effect of individual circadian preferences of drivers with fixed night work schedules on sleep patterns. Subjects and Methods: A total of 123 professional drivers, 32 indifferent preference drivers and 91 morning preference drivers of an intermunicipality and interstate bus transportation company were evaluated. All drivers underwent polysomnographic recordings after their shifts. Furthermore, they filled out a questionnaire that contained sociodemographic and health questions. the Home and Ostberg questionnaire was used to assess the subjects' morningness-eveningness preference. Results: the mean age was 42.54 +/- 6.98 years and 82 (66.66%) of the drivers had worked for = 15 years. A significant effect on rapid eye movement (REM) was observed in the morning preference drivers. They showed an increased sleep latency and an REM sleep percentage of 5% of the total REM time. This reveals a significant effect on sleep architecture associated with work time. Conclusion: the drivers reported that morning preference had a significant effect on their sleep pattern indicating less REM sleep and longer REM sleep latency in the morning preference group. Thus, it is important to evaluate interactions between individual aspects of health and other parameters, such as sleep quality and work organizational factors, to promote night shift workers' health and well-being. (C) 2013 S. Karger AG, BaselUniversidade Federal de São PauloAssociação Fundo de Incentivo à PesquisaCentro Multidisciplinar em Sonolencia e Acidentes (CEMSA)/Fundo de Incentivo a Pesquisa (AFIP)Centro de Estudo em Psicobiologia e Exercicio (CEPE)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Associacao Fundo Incent Pesquisa, Ctr Multidisciplinar Sonolencia & Acidentes, São Paulo, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilUNESP, Univ Estadual Paulista Julio de Mesquita Fillho, Assis, BrazilUniv Estadual Campinas, Fac Ciencias Aplicadas, Limeira, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilWeb of Scienc

Formação de micorriza em variedades de oliveira (Olea europaea L.) com potencial de cultivo em Minas Gerais

The olive tree, due to the high demand for the products olive and oil, is a relevant crop for the global food market. To improve its cultivation, increasing efforts have been invested in studies on biological factors of the soil-plant relationships, mainly with regard to the exploitation of microorganisms such as arbuscular mycorrhizal fungi (AMF). In Brazil however, further research is still needed, both under controlled and field conditions. In this sense, this study investigated the formation of extraradical mycelium and spore numbers and diversity of mycorrhiza, on 17 olive tree (Olea europaea L.) varieties. These varieties, considered promising for cultivation in the state of Minas Gerais, are maintained in the olive germplasm bank of the Agricultural Research Institute of Minas Gerais (EPAMIG), at the Experimental Farm Maria da Fé. The response of the olive varieties differed in terms of the level of extraradical mycelium and mycorrhiza formation, since root colonization in the field varied from 0.4% to 3.6%. Under the edaphoclimatic conditions of the study region, no influence of the olive tree varieties on the total number of spores and diversity of AMF was detected, among which the fungal species Glomus ambisporum and Acaulospora scrobiculata were identified most frequently on the roots of the analyzed olive trees.A oliveira, em razão da elevada demanda por produtos como azeitona e azeite de oliva, apresenta posição de destaque no mercado mundial de alimentos. Para o aprimoramento de seu cultivo, pesquisas relacionadas com fatores de ordem biológica da relação solo-planta estão ganhando enfoque, principalmente quanto ao uso de microrganismos como fungos micorrízicos arbusculares (FMAs). Contudo, há uma grande escassez de informação nessa área no Brasil, tanto em condições controladas quanto realizadas in situ. Nesse sentido, o presente trabalho visou avaliar a formação de propágulos (usando as características micélio extrarradicular, número e diversidade de esporos) de micorrizaem17 variedades de oliveira (Olea europaea L.). Essas variedades, com potencial de cultivo no estado de Minas Gerais, são mantidas no banco de germoplasma da Fazenda Experimental de Maria da Fé – EPAMIG (Empresa Brasileira de Pesquisa Agropecuária de Minas Gerais). Houve resposta distinta entre as variedades de oliveira para a formação de micélio extrarradicular e micorriza, sendo a colonização radicular in situ variada entre 0,4% e 3,6%. Nas condições edafoclimáticas da região de pesquisa não foi detectada nenhuma influência das variedades de oliveira sobre o número total de esporos e a diversidade de FMAs, sendo que as espécies Glomus ambisporum e Acaulospora scrobiculata foram identificadas com maior frequência nas raízes das oliveiras analisadas

A rare case of mucoepidermoid carcinoma ex-pleomorphic adenoma of the hard palate

Carcinoma Ex-Pleomorphic Adenoma (CExPA) is a salivary gland carcinoma derived from a primary or recurrent benign pleomorphic adenoma (PA) extremely rare in minor salivary glands. In this paper, we report the case of a male afrodescendant patient, 37 years old, presenting a palatal irregular nodular lesion with approximately 3.5 cm diameter. The lesion had over two years of evolution, but started growing faster and presenting pain and ulceration in the last two months. The incisional biopsy revealed a typical pleomorphic adenoma with focal areas of nests of epidermoid and mucous cells, as well as microcyst formations, resembling the mucoepidermoid carcinoma (MEC). Immunohistochemical analysis revealed positivity for CK7, CK13, CK 14, p63 and Ki67 (about 30%), whereas alpha-SMA was restricted to the PA component. The diagnosis was CExPA (MEC-type). A discussion on the histopathological and immunohistochemical criteria for differential diagnosis of CExPA is provided in this work, hoping to contribute to a better knowledge and understanding of this rare malignant tumor. Key words:Salivary gland neoplasms, pleomorphic adenoma, adenocarcinoma, mucoepidermoid carcinoma, pathology, differential diagnosis

Secondary infections in a cohort of patients with COVID-19 admitted to an intensive care unit: impact of gram-negative bacterial resistance

Some studies have shown that secondary infections during the COVID-19 pandemic may have contributed to the high mortality. Our objective was to identify the frequency, types and etiology of bacterial infections in patients with COVID-19 admitted to an intensive care unit (ICU) and to evaluate the results of ICU stay, duration of mechanical ventilation (MV) and in-hospital mortality. It was a single-center study with a retrospective cohort of patients admitted consecutively to the ICU for more than 48 h between March and May 2020. Comparisons of groups with and without ICU- acquired infection were performed. A total of 191 patients with laboratory-confirmed COVID-19 were included and 57 patients had 97 secondary infectious events. The most frequent agents were Acinetobacter baumannii (28.9%), Pseudomonas aeruginosa (22.7%) and Klebsiella pneumoniae (14.4%); multi-drug resistance was present in 96% of A. baumannii and in 57% of K. pneumoniae. The most prevalent infection was ventilator-associated pneumonia in 57.9% of patients with bacterial infections, or 17.3% of all COVID-19 patients admitted to the ICU, followed by tracheobronchitis (26.3%). Patients with secondary infections had a longer ICU stay (40.0 vs. 17 days; p < 0.001), as well as a longer duration of MV (24.0 vs 9.0 days; p= 0.003). There were 68 (35.6%) deaths overall, of which 27 (39.7%) patients had bacterial infections. Among the 123 survivors, 30 (24.4%) had a secondary infections (OR 2.041; 95% CI 1.080 - 3.859). A high incidence of secondary infections, mainly caused by gram-negative bacteria has been observed. Secondary infections were associated with longer ICU stay, MV use and higher mortality

Frequencia alelica dos locus DYS390, DYS391 e DYS393 em individuos brasileiros e sua aplicação a identificação humana

Orientador: Heloisa Amelia de Lima CastroTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: A identificação humana pode ser realizada por meio dos mais variados procedimentos técnicos, principalmente pela análise comparativa dos documentos do prontuário odontológico. Atualmente, as técnicas de biologia molecular têm se apresentado como o recurso comparativo mais eficaz, contudo parcela significativa desses métodos requer, além do estabelecimento prévio de parâmetros de coleta de material biológico, a verificação da freqüência alélica da população na qual será aplicada. Assim, o presente estudo procurou evidenciar a freqüência dos alelos em 3 lócus de STR do cromossomo Y (DYS390, DYS391 e DYS393) numa população de indivíduos brasileiros leucodérmicos. Para tanto, foram padronizados protocolos de coleta e armazenamento de diferentes materiais biológicos, e estabelecidas rotinas de extração e amplificação do DNA. Os resultados apresentaram o lócus DYS390 com freqüência nos alei os 21, 22, 23, 24, 25 e 26, sendo o alelo 24 o que apresentou maior freqüência desse lócus, com 46%; o STR DYS391 com presença nos alelos 8,9, 10, 11 12 e 13, tendo o alelo 11 incidência de 37% desse STR; e o STR DYS393 nos alelos 11, 12, 13 14 e 15, tendo o alelo 13 apresentado freqüência de 45% desse lócus. Esses dados, comparados àqueles de outras populações, demonstraram haver individualidade no perfil alélico da população estudada, comprovando que, mais do que aplicar as metodologias disponíveis, é prudente que se verifiquem os padrões dos alelos na população alvo, pois a utilização de padrões alélicos de populações diversas da nossa pode levar a imprecisões nos diagnósticos de identificação humanaAbstract: Human identification can be accomplished by several technical procedures, especially by the comparative analysis of dental documents. Nowadays, molecular biology introduced more effective resources for the human identification. However, these methods demand the previous definition of parameters to the collection of biological material, and the verification of the allele frequency of the population in which they will be applied. Aiming to subsidize these methods, we observed the frequency of the alleles in three loci of STRs of the Y chromosome (DYS390, DYS391 and DYS393) in a group of Brazilian white subjects. We also standardized protocols for the collection and storage of different biological materiais, besides defining routines for the extraction and amplification of the DNA. Results presented alleles 21, 22, 23, 24, 25 and 26 in the locus DYS390, being the allele 24 the most frequent with 46%. STR DYS391 presented alleles 8, 9, 10, 11 12 and 13, and the allele 11, with 37%, was the most frequent. STR DYS393 presented alleles 11, 12, 1314 and 15, and the allele 13, with 45%, was the mostfrequent. The comparative analysis of these data, with information related to other populations, stresses the singularity of the allele profile of the studied population. Therefore, prior to the introduction of recent methods of molecular biology to the human identification, we should carefully verify allele patterns of the population, because the use of allele standards of other populations could produce imprecision in human identification processesDoutoradoDoutor em Odontologia Legal e Deontologi

Estimativa do sexo atraves de mensurações mandibulares

Orientador: Roberto Jose GonçalvesDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: o presente trabalho teve como objetivo desenvolver metodologias para possibilitar a determinação do sexo através de mensurações mandibulares, verificando-se a aplicabilidade da metodologia encontrada na bibliografia, em padrões nacionais, como meio complementar no processo de identificação. Foram estudadas 175 mandíbulas, de indivíduos adultos (acima de 20 anos) de procedência e sexo conhecidos, independentemente da raça. O estudo baseou-se em quatro medidas a saber: a altura do ramo mandibular, o comprimento total da mandíbula, a distância bigoníaca e a largura mínima do ramo mandibular. Pela análise do dados, 'verificou-se que os resultados não coincidiam com os descritos na bibliografia, sendo que foi necessário o ajustamento do valor crítico limite entre os sexos. Além disso, estabeleceu-se, através de análises estatísticas, duas medidas que mostraram-se mais fidedignas em relação ao dimorfismo sexual, a saber: altura do ramo mandibular e distância bigoníaca. Foram também estabelecidas novas fórmulas, através da regressão logística e da análise discriminante, utilizando-se somente as duas medidas selecionadas. Foi ainda elaborado um # software" (Sexmandi), para a execução rápida, segura e confiável dos cálculos para determinação do sexo, por mensurações mandibulares, com obtenção de um escore de probabilidade numa taxa de acerto média de 77,7%Abstract: The purpose of this work is to study gender determination through mandibular mensurations. It also intends to verify the applicability of the methodology found in the bibliography in national standards as a supplementary way in the identification processo With this purpose we have studied 175 adult mandibles (above twenty) whose gender and origin were known, independent of their race. The study was based on four mensurations, namely: the height of the branch, the totallenth of the mandible, the bigonial distance and finally, the minimal width of the branch. The analysis of the dáta has shown that the results did not coincide with the ones found in the pertenent litrature. After having made the adjustment of the critica! value we have two similar results. During our research we concluded that the branchand and the bigonia! width are statistically more significant in relationto the sexual dimorphism and so we stablished new formulae, using only these two mensures. This study led to the development of a software (Sexmandi) for quick, safe and reliable performance of the calculation to determine sex through mandibular mensuratians with a rate af accuracy af 77,7% and the presentatian af a prabability scareMestradoOdontologia Legal e DeontologiaMestre em Ciência

Helium Cold Atmospheric Plasma Causes Morphological and Biochemical Alterations in <i>Candida albicans</i> Cells

(1) Background: Previous studies reported the promising inhibitory effect of cold atmospheric plasma (CAP) on Candida albicans. However, the exact mechanisms of CAP’s action on the fungal cell are still poorly understood. This study aims to elucidate the CAP effect on C. albicans cell wall, by evaluating the alterations on its structure and biochemical composition; (2) Methods: C. albicans cells treated with Helium-CAP were analyzed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in order to detect morphological, topographic and biochemical changes in the fungal cell wall. Cells treated with caspofungin were also analyzed for comparative purposes; (3) Results: Expressive morphological and topographic changes, such as increased roughness and shape modification, were observed in the cells after CAP exposure. The alterations detected were similar to those observed after the treatment with caspofungin. The main biochemical changes occurred in polysaccharides content, and an overall decrease in glucans and an increase in chitin synthesis were detected; (4) Conclusions: Helium-CAP caused morphological and topographic alterations in C. albicans cells and affected the cell wall polysaccharide content