The orchid bees (Hymenoptera, Apidae, Euglossina) in a forest fragment from western Paraná state, Brazil

An orchid bee inventory was carried out in Parque Estadual São Camilo, Palotina, Paraná (Brazil); conservation unit with about 400 hectares of Semidecidual Seasonal forest. Three bait traps were installed at the border of the fragment, each one containing the following fragrances: 1,8-cineole, eugenol, and vanilin. Sampling was carried out from 09am to 03pm, October 2011 to June 2012, summing up nine sampling days. A total of 186 specimens distributed among seven species were sampled. Eufriesea violacea with 140 specimens was the most common species, followed by Euglossa fimbriata (31), Euglossa annectans (9), Eulaema nigrita (4), Euglossa cordata (1), Euglossa pleosticta (1), and Exaerete smaragdina (1). According to qualitative and NMDS analysis, the orchid bee fauna of Parque Estadual São Camilo is representative of Semidecidual Seasonal forest, with richness comparable with other assemblages in the southern distribution of Euglossina. The sampled bee richness indicates that forest fragments, even small and isolated, are important in the conservation of this bees.Abelhas das orquídeas (Hymenoptera, Apidae, Euglossina) em um fragmento florestal do oeste do Paraná. Um inventário de abelhas das orquídeas foi conduzido no Parque Estadual São Camilo, Palotina, Paraná, uma unidade de conservação de cerca de 400 hectares de Floresta Estacional Semidecidual. Três garrafas-armadilha foram instaladas na borda do fragmento, cada uma contendo uma das seguintes essências: 1,8-cineol, eugenol e vanilina. A amostragem foi realizada entre 09 e 15 horas, entre outubro de 2011 e junho de 2012, somando nove dias amostrais. Um total de 186 espécimens distribuídos em sete espécies foram amostrados, Eufriesea violacea com 140 indivíduos foi a mais comum, seguida de Euglossa fimbriata com 31, Euglossa annectans com nove, Eulaema nigrita com quatro, Euglossa cordata, Euglossa pleosticta e Exaerete smaragdina com apenas um indivíduo amostrado cada. De acordo com análise qualitativa e de NMDS a fauna de Euglossina de Parque Estadual São Camilo é representativa de Floresta Estacional Semidecidual, com riqueza comparável a outras áreas no sul da distribuição do grupo. A riqueza de espécie amostrada indica que fragmentos florestais, mesmo que pequenos e isolados, são importantes na conservação destas abelhas

Water quantity and quality under future climate and societal scenarios: a basin-wide approach applied to the Sorraia river, Portugal

Water resources are impacted by several stressors like over-population and over consumption that compromises their availability. These stressors are expected to progressively intensify due to climate change in most regions of the world, with direct impact on watersheds and river systems. This study investigates the effect of different watershed pressure scenarios due to climate change in the hydrological regime of the Sorraia River basin, Portugal. This catchment includes one of the largest irrigated areas in the country, thus being strongly influenced by anthropogenic activities, associated to hydrological (irrigation, flow regulation, damming) and nutrient stressors. The SoilWater Assessment Tool has been used to simulate water flow and nutrient dynamics in the watershed while considering inputs from two climate models and three societal scenarios. Results have shown that the predicted rainfall reductions will have a significant impact on river flow and nutrient concentrations when compared to baseline conditions. River flow will expectably decrease by 75%, while nitrogen and phosphorus concentrations in river water will expectably increase by 500% and 200%, respectively. These differences are more evident for storylines that consider increasing pressures such as population growth and agricultural expansion marked with unsustainable practices and increased reliance on technology. The results of this study indicate a possible future outcome and provide effective guidelines for the formulation of water management policies to counter the impacts of climate change and corresponding environmental pressures in the Sorraia River basininfo:eu-repo/semantics/publishedVersio

Evaluation of the trophic status in a Mediterranean reservoir under climate change: an integrated modelling approach

This study describes an integrated modelling approach to better understand the trophic status of the Montargil reservoir (southern Portugal) under climate change scenarios. The SWAT and CE-QUAL-W2 models were applied to the basin and reservoir, respectively, for simulating water and nutrient dynamics while considering one climatic scenario and two decadal timelines (2025–2034 and 2055–2064). Model simulations showed that the dissolved oxygen concentration in the reservoir’s hypolimnion is expected to decrease by 60% in both decadal timelines, while the chlorophyll-a concentration in the reservoir’s epiliminion is expected to increase by 25%. The total phosphorus concentration (TP) is predicted to increase in the water column surface by 63% and in the hypolimion by 90% during the 2030 timeline. These results are even more severe during the 2060 timeline. Under this climate change scenario, the reservoir showed a eutrophic state during 70–80% of both timelines. Even considering measures that involve decreases in 30 to 35% of water use, the eutrophic state is not expected to improveinfo:eu-repo/semantics/publishedVersio

Efeitos dos exercícios pilates na função do tronco e na dor de pacientes com lombalgia / Effects of pilates exercices on trunk function and pain in patients with low back pain

Introdução: Evidências científi cas atuais apontam que a falta de resistência da musculatura lombar é o principal fator para desenvolvimento das dores lombares. O exercício físico em geral tem se mostrado benéfi co para recuperação da funcionalidade bem como para melhora dos sintomas clínicos dos pacientes com lombalgia crônica. Objetivo: Avaliar os efeitos de um treinamento físico por meio dos exercícios de Pilates de solo nas variáveis de dor, flexibilidade, equilíbrio, força e resistência dos músculos lombares em pacientes com lombalgia de origem desconhecida. Método: Foram avaliados 12 pacientes com lombalgia crônica (idade média de 33 anos), que realizaram um período de treinamento com o método tradicional de 13 exercícios Pilates (básico e intermediário no mat), duas vezes na semana, com duração de uma hora, durante um total de 11 semanas. As principais medidas de resultados analisadas pré- e pós-treinamento Pilates foram: (1) flexibilidade (Banco de Wells), (2) equilíbrio unipodal (plataforma de força), (3) força lombar (dinamometria), (4) resistência lombar (teste de Sorensen), e dor (Escala Visual Analógica: EVA). Resultados: A amostra fi nal do estudo foi de quatro voluntários devido à perda experimental. Os resultados mostraram uma diminuição significativa da dor (P < 0.05), mas nenhuma diferença significativa entre pré- e pós-treinamento Pilates para as demais variáveis, apesar da leve melhora na fl exibilidade e força. Conclusão: Os resultados do presente estudo apresentaram efeitos benéfi cos do treinamento Pilates para diminuir os sintomas de dor. Entretanto, novos estudos com maior número amostral devem ser realizados para melhor estabelecer os protocolos de exercícios Pilates em pacientes com lombalgia crônica. Introduction: Scientific evidences report that poor back muscle endurance is the most risk factor for developing low back pain. Overall, physical exercise has been effective to recovery of the functioning as well as to improve the clinical symptoms of patients with low back pain. Objective: To assess the effects of Pilates training on mat in variables of flexibility, balance, strength and endurance of the lumbar extensor muscles in patients with low back pain unknown. Method: 16 patients with chronic low back pain (mean age 33 yrs) performed a training program with a mat method of 13 Pilates exercises (basic and intermediate), 2 x a week for a session of approximately 1h, during 11 weeks. The main outcome measures analyzed before (pre-) and post-Pilates training were: fl exibility (sit-and-reach during a Wells test), unipodal support (under a force platform), strength (lumbar dynamometer) and endurance (Sorensen test) of lumbar muscles and pain (VAS). Results: Significant decrease of the pain was found (P < 0.05). However, no significant changes were found for other variables analyzing in pre- and post-training, although the slight improvement in the flexibility and strength. Conclusions: The results of the present study showed the effi cacy of the Pilates exercises to decrease pain. However, more studies would be conducted using much more subjects to establish better standardization of Pilates exercises in patients with low back pain

High Avidity dsDNA Autoantibodies in Brazilian Women with Systemic Lupus Erythematosus: Correlation with Active Disease and Renal Dysfunction

We investigated in Brazilian women with SLE the prevalence and levels of high avidity (HA) dsDNA antibodies and tested their correlation with lupus activity and biomarkers of renal disease. We also compared these correlations to those observed with total dsDNA antibodies and antibodies against nucleosome (ANuA). Autoantibodies were detected by ELISA, while C3 and C4 levels were determined by nephelometry. Urine protein/creatinine ratio was determined, and lupus activity was measured by SLEDAI-2K. The prevalence of total and HA dsDNA antibodies was similar to but lower than that verified for ANuA. The levels of the three types of antibodies were correlated, but the correlation was more significant between HA dsDNA antibodies and ANuA. High avidity dsDNA antibodies correlated positively with ESR and SLEDAI and inversely with C3 and C4. Similar correlations were observed for ANuA levels, whereas total dsDNA antibodies only correlated with SLEDAI and C3. The levels of HA dsDNA antibodies were higher in patients with proteinuria, but their levels of total dsDNA antibodies and ANuA were unaltered. High avidity dsDNA antibodies can be found in high prevalence in Brazilian women with SLE and are important biomarkers of active disease and kidney dysfunction

Biotransformation of digitoxigenin by Cochliobolus lunatus

The biotransformation of digitoxigenin (1) by Cochliobolus lunatus was investigated. The biotransformation reaction was carried out in a 4-day process, resulting in the isolation of four products, whose structures were elucidated as 1beta-hydroxydigitoxigenin (2), 7beta-hydroxydigitoxigenin (3), 8beta-hydroxydigitoxigenin (4) and digitoxigenone (5). The production of these derivatives under the employed conditions has never been described so far. This is also the first report on the production of compound 4 by a biotransformation reaction

Macaúba (Acrocomia aculeata) cake from biodiesel processing: a low-cost substrate to produce lipases from Moniliella spathulata R25L270 with potential application in the oleochemical industry

[Background]: Biodiesel industry wastes were evaluated as supplements for lipase production by Moniliella spathulata R25L270, which is newly identified yeast with great lipolytic potential. Macaúba cake (MC), used for the first time in this work as inducer to produce lipases, and residual oil (RO) were mixed to maximise enzyme production. The lipase secreted was biochemically characterised.[Results]: The best ratio for the mixture (MC:RO) was 0.66:0.34 and the fitted values for lipase activity and total protein concentration were 0.98 U mL−1 and 0.356 mg mL−1, respectively. Maximum activity obtained (2.47 U mL−1) was achieved at 31.5°C and pH 6.7, and the enzyme was stable in this condition. A novel enzyme was purified and identified for the first time by mass spectrometry. The lipase efficiently hydrolysed different natural oils and exhibited selectivity in the production of eicosapentaenoic acid from fish oil.[Conclusion]: The use of MC and RO as a supplement to produce the new lipase from M. spathulata R25L270 may be one alternative for reducing lipase production costs and simultaneously adding value to biodiesel industry residues. The potential application of the lipase in the oleochemical industry was demonstrated by its pH and temperature stabilities and selective hydrolysis.This research was supported by Brazilian agencies: CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), INCT (Instituto Nacional de Ciência e Tecnologia) de Nanomateriais de Carbono, FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais), Rede Mineira de Toxinas com Ação Terapêutica and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).Peer reviewe

Is urinary density an adequate predictor of urinary osmolality?

Urinary density (UD) has been routinely used for decades as a surrogate marker for urine osmolality (U-osm). We asked if UD can accurately estimate U-osm both in healthy subjects and in different clinical scenarios of kidney disease. UD was assessed by refractometry. U-osm was measured by freezing point depression in spot urines obtained from healthy volunteers (N = 97) and in 319 inpatients with acute kidney injury (N = 95), primary glomerulophaties (N = 118) or chronic kidney disease (N = 106). UD and U-osm correlated in all groups (p < 0.05). However, a wide range of U-osm values was associated with each UD value. When UD was <= 1.010, 28.4% of samples had U-osm above 350 mOsm/kg. Conversely, in 61.6% of samples with UD above 1.020, U-osm was below 600 mOsm/kg. As expected, U-osm exhibited a strong relationship with serum creatinine (S-creat), whereas a much weaker correlation was found between UD and Screat. We found that UD is not a substitute for U-osm. Although UD was significantly correlated with U-osm, the wide dispersion makes it impossible to use UD as a dependable clinical estimate of U-osm. Evaluation of the renal concentrating ability should be based on direct determination of U-osm1