Possible horizontal transfer of two subclasses of Mutator-like elements within Poaceae

Dissertação de mestrado em Gestão da Formação e Administração Educacional (Organizações Educativas e Gestão Escolar), apresentada à Faculdade de Psicologia e de Ciências da Educação da Universidade de CoimbraA presente dissertação é resultado de uma investigação realizada num Agrupamento de Escolas na zona Centro que obteve uma melhoria entre o primeiro e o segundo ciclo de avaliação externa de escolas. Centra-se na liderança praticada nas organizações escolares e na relação entre a melhoria das escolas com o Programa de Avaliação Externa de Escolas, com vista a compreender o papel das lideranças na melhoria de uma organização. A avaliação externa de escolas tem-se tornado indispensável para a criação de mecanismos de regulação que, de forma integrada com o processo de autoavaliação realizado pelas escolas desenvolvem novas práticas e, consequentemente, implementam processos de melhoria, visíveis pelas tomadas de decisão dos líderes das escolas. Com vista a verificar o que as lideranças do agrupamento têm feito para melhorar os resultados dos alunos, a prestação do serviço educativo e a capacidade de autoavaliação e melhoria desde a primeira avaliação externa, optámos pela metodologia de estudo de caso, procurando conjugar técnicas de natureza quantitativa e qualitativa. Neste sentido, recorremos à análise dos documentos estruturantes do Agrupamento de Escolas (Projeto Educativo, Regulamento Interno, Plano Anual de Atividades, Plano de Melhoria, Relatórios de Avaliação externa e Contraditório), à realização de entrevistas com o Diretor e com a Coordenadora da Equipa de Autoavaliação e, por fim, à elaboração e administração de um questionário para conhecer as perspetivas dos docentes sobre o funcionamento e o desempenho do Agrupamento de Escolas, bem como sobre as mudanças ocorridas na sequência da avaliação externa de escolas (AEE). Este estudo salienta que o papel de uma liderança efetiva e reconhecida é parte fundamental no desenvolvimento de uma organização e que a Avaliação Externa de Escolas é sentida e vivenciada pela organização em estudo, e ao ser conjugada com os dispositivos de reflexão interna e de autoavaliação, incentiva ao aperfeiçoamento dos planos de melhoria, encaminhando o Agrupamento para a melhoria contínua da qualidade do seu serviço educativo.The following dissertation is the result of an investigation in a School’s Division from center area of Portugal that has improved consederably its results in the first and second level of the External Evaluation of Schools. Its main focus is on the leadership of the Schools Organizations and the relationship between the Schools Improvement and the External Evaluation Program from Schools, taking in consideration the role of leadership in the improvement of an Organization. The external evaluation of schools has become, without doubt, necessary to create regulamentation mechanisms in order that schools could develop new cope ways and therefore, to implement and establish netter working processes, acknowledged by the schools chairmen and directors. With the main interes of checking the School’s Division’s Leadership and their work to improve the capability of self-evaluation, we opted to user a methodological study over quality and quantity. In this matter we chose to study and analyse the School’s Division documents, such as Educative Projects, Internal Regulation, Activities Annual Planification, Effort Planification and External Evaluation Reports. We also took action in interviewing School Directors, the self-evaluation Team Coordinator and foremost to create and practice a questionnaire to acknowledge the teacher’s perspectives about School’s Division maintenance and development, as well as the School’s External Evaluation . This study case empathizes the role of an effective and recognised leadership. It’s essential and crucial to the welfare and development of an organisation and the External School’s Evaluation is taken in great consideration by our study case. Moreover, dued to the self-evaluation system and combined with internal reflection devices it enhances the leadership’s accuracy in the quality improvement.La dissertation suivante est le résultat d'une investigation réalisée sur un ensemble d'écoles de la région centre du pays dont on a observé une amélioration entre le premier et le second cycle de l'évaluation externe des écoles. Elle porte sur la direction des organisations scolaires et le lien avec l'amélioration apportée aux écoles participant au programme d'évaluation externe des écoles. Elle vise ainsi à comprendre le rôle des directions dans la progression d'une institution. L'évaluation externe des écoles est devenue indispensable à la création de mécanismes de régulation, qui, intégrés au processus d'auto évaluation réalisée par l’école, permettent de développer de nouvelles méthodes. C'est ainsi par conséquent que sont mis en place des systèmes d'amélioration, rendus visibles grâce aux prises de décision des responsables désignés de l’établissement scolaire. Dans le but de vérifier la stratégie adoptée par les directions dans l’amélioration des résultats scolaire des élèves, la qualité du service éducatif et la capacité d’auto-évaluation depuis la première évaluation externe, nous choisirons de présenter ce travail à travers une étude de cas, cherchant ainsi à concilier des méthodes à la fois quantitative et qualitative. Ainsi, nous avons recours à une analyse de documents d’ordre organisationnel de l’ensemble scolaire (projet éducatif, règlement intérieur, plan annuel d’activités, plan d’amélioration, comptes rendus de l’évaluation externe et contradictoire), à la réalisation de rencontres avec le directeur et la coordinatrice de l’équipe d’autoévaluation et, enfin, à l’élaboration d’un questionnaire visant à connaitre les projets à venir dans le futur fonctionnement et l’investissement de l’ensemble d’écoles, tout comme dans les changements réalisés dans la séquence d’évaluation externe des écoles. Cette étude démontre que, le rôle d’une direction effective et reconnue est fondamentale dans le développement d’une organisation; et que l’évaluation externe des écoles prend tout son sens dans l’ensemble d’étude. De plus, l’ajout des dispositifs de réflexion interne et d’autoévaluation, facilite grandement le perfectionnement des plans d’amélioration, permettant ainsi l’amélioration constante et continue de la qualité du service éducatif de cet ensemble scolaire

Primary and malignant cholangiocytes undergo CD40 mediated Fas dependent Apoptosis, but are insensitive to direct activation with exogenous fas ligand

Introduction Cholangiocarcinoma is a rare malignancy of the biliary tract, the incidence of which is rising, but the pathogenesis of which remains uncertain. No common genetic defects have been described but it is accepted that chronic inflammation is an important contributing factor. We have shown that primary human cholangiocyte and hepatocyte survival is tightly regulated via co-operative interactions between two tumour necrosis family (TNF) receptor family members; CD40 and Fas (CD95). Functional deficiency of CD154, the ligand for CD40, leads to a failure of clearance of biliary tract infections and a predisposition to cholangiocarcinoma implying a direct link between TNF receptor-mediated apoptosis and the development of cholangiocarcinoma. Aims To determine whether malignant cholangiocytes display defects in CD40 mediated apoptosis. By comparing CD40 and Fas-mediated apoptosis and intracellular signalling in primary human cholangiocytes and three cholangiocyte cell lines. Results Primary cholangiocytes and cholangiocyte cell lines were relatively insensitive to direct Fas-mediated killing with exogenous FasL when compared with Jurkat cells, which readily underwent Fas-mediated apoptosis, but were extremely sensitive to CD154 stimulation. The sensitivity of cells to CD40 activation was similar in magnitude in both primary and malignant cells and was STAT-3 and AP-1 dependent in both. Conclusions 1) Both primary and malignant cholangiocytes are relatively resistant to Fas–mediated killing but show exquisite sensitivity to CD154, suggesting that the CD40 pathway is intact and fully functional in both primary and malignant cholangiocytes 2) The relative insensitivity of cholangiocytes to Fas activation demonstrates the importance of CD40 augmentation of Fas dependent death in these cells. Agonistic therapies which target CD40 and associated intracellular signalling pathways may be effective in promoting apoptosis of malignant cholangiocytes

Flow angle from intermediate mass fragment measurements

Directed sideward flow of light charged particles and intermediate mass fragments was measured in different symmetric reactions at bombarding energies from 90 to 800 AMeV. The flow parameter is found to increase with the charge of the detected fragment up to Z = 3-4 and then turns into saturation for heavier fragments. Guided by simple simulations of an anisotropic expanding thermal source, we show that the value at saturation can provide a good estimate of the flow angle, $\Theta_{flow}$, in the participant region. It is found that $\Theta_{flow}$ depends strongly on the impact parameter. The excitation function of $\Theta_{flow}$ reveals striking deviations from the ideal hydrodynamical scaling. The data exhibit a steep rise of \Theta_{\flow} to a maximum at around 250-400 AMeV, followed by a moderate decrease as the bombarding energy increases further.Comment: 28 pages Revtex, 6 figures (ps files), to appear in Nucl.Phys.

The comparative anatomy of the folds, fossae, and adhesions around the duodenojejunal flexure in mammals

Background: Anatomical knowledge of the duodenojejunal flexure is necessary for abdominal surgeries, and also important for physiologic studies about the duodenum. But little is known about the anatomy of this region in mammals. Here, we examined comparative anatomy to understand the anatomical formation of the duodenojejunal flexure in mammals. Materials and methods: The areas around the duonenojejunal flexure were ob­served in mouse, rat, dog, pig, and human, and the anatomical structures around the duodenojejunal junction in the animals were compared with those in human. Results: The superior and inferior duodenal folds, and the superior and inferior duodenal fossae were identified in all examined humans. In pig, the structures were not clearly identified because the duodenum strongly adhered to the retroperitoneum and to the mesocolon. In mouse, rat, and dog, only the plica duodenocolica, which is regarded as the animal counterpart of the superior duo­denal fold in human, was identified, and other folds or fossae were not observed, probably because the duodenum was not fixed to the parietal peritoneum in those animals. Transection of the plica duodenocolica could return the normally rotated intestine back to the state of non-rotation in rat. Conclusions: This study showed the anatomical similarities and dissimilarities of the duodenojejunal flexure among the mammals. Anatomical knowledge of the area is useful for duodenal and pancreatic surgeries, and for animal studies about the duodenum. (Folia Morphol 2018; 77, 2: 286–292

Substrate specificity of microbial transglutaminase as revealed by three-dimensional docking simulation and mutagenesis

Transglutaminases (TGases) are used in fields such as food and pharmaceuticals. Unlike other TGases, microbial transglutaminase (MTG) activity is Ca2+-independent, broadening its application. Here, a three-dimensional docking model of MTG binding to a peptide substrate, CBZ-Gln-Gly, was simulated. The data reveal CBZ-Gln-Gly to be stretched along the MTG active site cleft with hydrophobic and/or aromatic residues interacting directly with the substrate. Moreover, an oxyanion binding site for TGase activity may be constructed from the amide groups of Cys64 and/or Val65. Alanine mutagenesis verified the simulated binding region and indicated that large molecules can be widely recognized on the MTG cleft

Role of cellular senescence and NOX4-mediated oxidative stress in systemic sclerosis pathogenesis.

Systemic sclerosis (SSc) is a systemic autoimmune disease characterized by progressive fibrosis of skin and numerous internal organs and a severe fibroproliferative vasculopathy resulting frequently in severe disability and high mortality. Although the etiology of SSc is unknown and the detailed mechanisms responsible for the fibrotic process have not been fully elucidated, one important observation from a large US population study was the demonstration of a late onset of SSc with a peak incidence between 45 and 54 years of age in African-American females and between 65 and 74 years of age in white females. Although it is not appropriate to consider SSc as a disease of aging, the possibility that senescence changes in the cellular elements involved in its pathogenesis may play a role has not been thoroughly examined. The process of cellular senescence is extremely complex, and the mechanisms, molecular events, and signaling pathways involved have not been fully elucidated; however, there is strong evidence to support the concept that oxidative stress caused by the excessive generation of reactive oxygen species may be one important mechanism involved. On the other hand, numerous studies have implicated oxidative stress in SSc pathogenesis, thus, suggesting a plausible mechanism in which excessive oxidative stress induces cellular senescence and that the molecular events associated with this complex process play an important role in the fibrotic and fibroproliferative vasculopathy characteristic of SSc. Here, recent studies examining the role of cellular senescence and of oxidative stress in SSc pathogenesis will be reviewed