Differential p38-dependent signalling in response to cellular stress and mitogenic stimulation in fibroblasts

p38 MAP kinase is known to be activated by cellular stress finally leading to cell cycle arrest or apoptosis. Furthermore, a tumour suppressor role of p38 MAPK has been proposed. In contrast, a requirement of p38 for proliferation has also been described. To clarify this paradox, we investigated stress- and mitogen-induced p38 signalling in the same cell type using fibroblasts. We demonstrate that - in the same cell line - p38 is activated by mitogens or cellular stress, but p38-dependent signalling is different. Exposure to cellular stress, such as anisomycin, leads to a strong and persistent p38 activation independent of GTPases. As a result, MK2 and downstream the transcription factor CREB are phosphorylated. In contrast, mitogenic stimulation results in a weaker and transient p38 activation, which upstream involves small GTPases and is required for cyclin D1 induction. Consequently, the retinoblastoma protein is phosphorylated and allows G1/S transition. Our data suggest a dual role of p38 and indicate that the level and/or duration of p38 activation determines the cellular response, i.e either proliferation or cell cycle arrest

Expanded Search for z~10 Galaxies from HUDF09, ERS, and CANDELS Data: Evidence for Accelerated Evolution at z>8?

We search for z~10 galaxies over ~160 arcmin^2 of WFC3/IR data in the Chandra Deep Field South, using the public HUDF09, ERS, and CANDELS surveys, that reach to 5sigma depths ranging from 26.9 to 29.4 in H_160 AB mag. z>~9.5 galaxy candidates are identified via J_125-H_160>1.2 colors and non-detections in any band blueward of J_125. Spitzer IRAC photometry is key for separating the genuine high-z candidates from intermediate redshift (z~2-4) galaxies with evolved or heavily dust obscured stellar populations. After removing 16 sources of intermediate brightness (H_160~24-26 mag) with strong IRAC detections, we only find one plausible z~10 galaxy candidate in the whole data set, previously reported in Bouwens et al. (2011). The newer data cover a 3x larger area and provide much stronger constraints on the evolution of the UV luminosity function (LF). If the evolution of the z~4-8 LFs is extrapolated to z~10, six z~10 galaxies are expected in our data. The detection of only one source suggests that the UV LF evolves at an accelerated rate before z~8. The luminosity density is found to increase by more than an order of magnitude in only 170 Myr from z~10 to z~8. This increase is >=4x larger than expected from the lower redshift extrapolation of the UV LF. We are thus likely witnessing the first rapid build-up of galaxies in the heart of cosmic reionization. Future deep HST WFC3/IR data, reaching to well beyond 29 mag, can enable a more robust quantification of the accelerated evolution around z~10.Comment: 13 pages, 11 figures, ApJ resubmitted after referee repor

Very blue UV-continuum slopes of low luminosity z~7 galaxies from WFC3/IR: Evidence for extremely low metallicities?

We use the ultra-deep WFC3/IR data over the HUDF and the Early Release Science WFC3/IR data over the CDF-South GOODS field to quantify the broadband spectral properties of candidate star-forming galaxies at z~7. We determine the UV-continuum slope beta in these galaxies, and compare the slopes with galaxies at later times to measure the evolution in beta. For luminous L*(z=3) galaxies, we measure a mean UV-continuum slope beta of -2.0+/-0.2, which is comparable to the beta~-2 derived at similar luminosities at z~5-6. However, for the lower luminosity 0.1L*(z=3) galaxies, we measure a mean beta of -3.0+/-0.2. This is substantially bluer than is found for similar luminosity galaxies at z~4, just 800 Myr later, and even at z~5-6. In principle, the observed beta of -3.0 can be matched by a very young, dust-free stellar population, but when nebular emission is included the expected beta becomes >~-2.7. To produce these very blue beta's (i.e., beta~-3), extremely low metallicities and mechanisms to reduce the red nebular emission are likely required. For example, a large escape fraction (i.e., f_{esc}>~0.3) could minimize the contribution from this red nebular emission. If this is correct and the escape fraction in faint z~7 galaxies is >~0.3, it may help to explain how galaxies reionize the universe.Comment: 5 pages, 5 figures, accepted for publication in Astrophysical Journal Letter

UV Luminosity Functions from 132 z~7 and z~8 Lyman-Break Galaxies in the ultra-deep HUDF09 and wide-area ERS WFC3/IR Observations

We identify 73 z~7 and 59 z~8 candidate galaxies in the reionization epoch, and use this large 26-29.4 AB mag sample of galaxies to derive very deep luminosity functions to <-18 AB mag and the star formation rate density at z~7 and z~8. The galaxy sample is derived using a sophisticated Lyman-Break technique on the full two-year WFC3/IR and ACS data available over the HUDF09 (~29.4 AB mag, 5 sigma), two nearby HUDF09 fields (~29 AB mag, 14 arcmin) and the wider area ERS (~27.5 AB mag) ~40 arcmin**2). The application of strict optical non-detection criteria ensures the contamination fraction is kept low (just ~7% in the HUDF). This very low value includes a full assessment of the contamination from lower redshift sources, photometric scatter, AGN, spurious sources, low mass stars, and transients (e.g., SNe). From careful modelling of the selection volumes for each of our search fields we derive luminosity functions for galaxies at z~7 and z~8 to <-18 AB mag. The faint-end slopes alpha at z~7 and z~8 are uncertain but very steep at alpha = -2.01+/-0.21 and alpha=-1.91+/-0.32, respectively. Such steep slopes contrast to the local alpha<~-1.4 and may even be steeper than that at z~4 where alpha=-1.73+/-0.05. With such steep slopes (alpha<~-1.7) lower luminosity galaxies dominate the galaxy luminosity density during the epoch of reionization. The star formation rate densities derived from these new z~7 and z~8 luminosity functions are consistent with the trends found at later times (lower redshifts). We find reasonable consistency, with the SFR densities implied from reported stellar mass densities, being only ~40% higher at z<7. This suggests that (1) the stellar mass densities inferred from the Spitzer IRAC photometry are reasonably accurate and (2) that the IMF at very high redshift may not be very different from that at later times.Comment: 38 pages, 21 figures, 20 tables, ApJ, accepted for publicatio

Overdensities of Y-dropout Galaxies from the Brightest-of-Reionizing Galaxies Survey: A Candidate Protocluster at Redshift z~8

Theoretical and numerical modeling of dark-matter halo assembly predicts that the most luminous galaxies at high redshift are surrounded by overdensities of fainter companions. We test this prediction with HST observations acquired by our Brightest of Reionizing Galaxies (BoRG) survey, which identified four very bright z~8 candidates as Y-dropout sources in four of the 23 non-contiguous WFC3 fields observed. We extend here the search for Y-dropouts to fainter luminosities (M_* galaxies with M_AB\sim-20), with detections at >5sigma confidence (compared to >8sigma confidence adopted earlier) identifying 17 new candidates. We demonstrate that there is a correlation between number counts of faint and bright Y-dropouts at >99.84% confidence. Field BoRG58, which contains the best bright z\sim8 candidate (M_AB=-21.3), has the most significant overdensity of faint Y-dropouts. Four new sources are located within 70arcsec (corresponding to 3.1 comoving Mpc at z=8) from the previously known brighter z\sim8 candidate. The overdensity of Y-dropouts in this field has a physical origin to high confidence (p>99.975%), independent of completeness and contamination rate of the Y-dropout selection. We modeled the overdensity by means of cosmological simulations and estimate that the principal dark matter halo has mass M_h\sim(4-7)x10^11Msun (\sim5sigma density peak) and is surrounded by several M_h\sim10^11Msun halos which could host the fainter dropouts. In this scenario, we predict that all halos will eventually merge into a M_h>2x10^14Msun galaxy cluster by z=0. Follow-up observations with ground and space based telescopes are required to secure the z\sim8 nature of the overdensity, discover new members, and measure their precise redshift.Comment: Minor revision: ApJ accepted [17 pages (emulateapj style), 7 figures, 2 tables

Ultradeep Infrared Array Camera Observations of sub-L* z~7 and z~8 Galaxies in the Hubble Ultra Deep Field: the Contribution of Low-Luminosity Galaxies to the Stellar Mass Density and Reionization

We study the Spitzer Infrared Array Camera (IRAC) mid-infrared (rest-frame optical) fluxes of 14 newly WFC3/IR-detected z=7 z_{850}-dropout galaxies and 5 z=8 Y_{105}-dropout galaxies. The WFC3/IR depth and spatial resolution allow accurate removal of contaminating foreground light, enabling reliable flux measurements at 3.6 micron and 4.5 micron. None of the galaxies are detected to [3.6]=26.9 (AB, 2 sigma), but a stacking analysis reveals a robust detection for the z_{850}-dropouts and an upper limit for the Y_{105}-dropouts. We construct average broadband SEDs using the stacked ACS, WFC3, and IRAC fluxes and fit stellar population synthesis models to derive mean redshifts, stellar masses, and ages. For the z_{850}-dropouts, we find z=6.9^{+0.1}_{-0.1}, (U-V)_{rest}=0.4, reddening A_V=0, stellar mass M*=1.2^{+0.3}_{-0.6} x 10^9 M_sun (Salpeter IMF). The best-fit ages ~300Myr, M/L_V=0.2, and SSFR=1.7Gyr^{-1} are similar to values reported for luminous z=7 galaxies, indicating the galaxies are smaller but not younger. The sub-L* galaxies observed here contribute significantly to the stellar mass density and under favorable conditions may have provided enough photons for sustained reionization at 7<z<11. In contrast, the z=8.3^{+0.1}_{-0.2} Y_{105}-dropouts have stellar masses that are uncertain by 1.5 dex due to the near-complete reliance on far-UV data. Adopting the 2 sigma upper limit on the M/L(z=8), the stellar mass density to M_{UV,AB} < -18 declines from rho*(z=7)=3.7^{+1.0}_{-1.8} x 10^6 M_sun Mpc^{-3} to rho*(z=8) < 8 x 10^5 M_sun Mpc^{-3}, following (1+z)^{-6} over 3<z<8. Lower masses at z=8 would signify more dramatic evolution, which can be established with deeper IRAC observations, long before the arrival of the James Webb Space Telescope.Comment: 6 pages, 3 figures, 2 tables, emulateapj, accepted for publication in ApJ

The Luminosity Function at z~8 from 97 Y-band dropouts: Inferences About Reionization

[Abbreviated] We present the largest search to date for $z\sim8$ Lyman break galaxies (LBGs) based on 350 arcmin$^2$ of HST observations in the V-, Y-, J- and H-bands from the Brightest of Reionizing Galaxies (BoRG) survey. The BoRG dataset includes $\sim$50 arcmin$^2$ of new data and deeper observations of two previous BoRG pointings, from which we present 9 new $z\sim8$ LBG candidates, bringing the total number of BoRG LBGs to 38 with $25.5\leqslant m_{J} \leqslant 27.6$ (AB system). We introduce a new Bayesian formalism for estimating the galaxy luminosity function (LF), which does not require binning (and thus smearing) of the data and includes a likelihood based on the formally correct binomial distribution as opposed to the often used approximate Poisson distribution. We demonstrate the utility of the new method on a sample of $97$ LBGs that combines the bright BoRG galaxies with the fainter sources published in Bouwens et al. (2012) from the HUDF and ERS programs. We show that the $z\sim8$ LF is well described by a Schechter function with a characteristic magnitude $M^\star = -20.15^{+0.29}_{-0.38}$, a faint-end slope of $\alpha = -1.87^{+0.26}_{-0.26}$, and a number density of $\log_{10} \phi^\star [\textrm{Mpc}^{-3}] = -3.24^{+0.25}_{-0.24}$. Integrated down to $M=-17.7$ this LF yields a luminosity density, $\log_{10} \epsilon [\textrm{erg}/\textrm{s/Hz/Mpc}^{3}] = 25.52^{+0.05}_{-0.05}$. Our LF analysis is consistent with previously published determinations within 1$\sigma$. We discuss the implication of our study for the physics of reionization. By assuming theoretically motivated priors on the clumping factor and the photon escape fraction we show that the UV LF from galaxy samples down to $M=-17.7$ can ionize only 10-50% of the neutral hydrogen at $z\sim8$. Full reionization would require extending the LF down to $M=-15$.Comment: Accepted for publication in ApJ, 22 pages, 15 figure

The bright-end galaxy candidates at z ~ 9 from 79 independent HST fields

We present a full data analysis of the pure-parallel Hubble Space Telescope (HST) imaging observations in the Brightest of Reionizing Galaxies Survey (BoRG[z9]) in Cycle 22. The medium-deep exposures with five HST/WFC3IR+UVIS filter bands from 79 independent sightlines (~370 arcmin^2) provide the least biased determination of number density for z>9 bright galaxies against cosmic variance. After a strict two-step selection for candidate galaxies, including dropout color and photometric redshift analyses, and revision of previous BoRG candidates, we identify one source at z~10 and two sources at z~9. The z~10 candidate shows evidence of line-of-sight lens magnification (mu~1.5), yet it appears surprisingly luminous (MUV ~ -22.6\pm0.3 mag), making it one of the brightest candidates at z > 8 known (~ 0.3 mag brighter than the z = 8.68 galaxy EGSY8p7, spectroscopically confirmed by Zitrin and collaborators). For z ~ 9 candidates, we include previous data points at fainter magnitudes and find that the data are well fitted by a Schechter luminosity function with alpha ~ -2.1, MUV ~ -21.5 mag, and log phi ~ -4.5 Mpc^-3mag^-1, for the first time without fixing any parameters. The inferred cosmic star formation rate density is consistent with unaccelerated evolution from lower redshift.Comment: 18pages, 7figures, 6tables. accepted to the Astrophysical Journa