Prescription of rhythmic patterns for legged locomotion

As the engine behind many life phenomena, motor information generated by the central nervous system (CNS) plays a critical role in the activities of all animals. In this work, a novel, macroscopic and model-independent approach is presented for creating different patterns of coupled neural oscillations observed in biological central pattern generators (CPG) during the control of legged locomotion. Based on a simple distributed state machine, which consists of two nodes sharing pre-defined number of resources, the concept of oscillatory building blocks (OBBs) is summarised for the production of elaborated rhythmic patterns. Various types of OBBs can be designed to construct a motion joint of one degree-of-freedom (DOF) with adjustable oscillatory frequencies and duty cycles. An OBBs network can thus be potentially built to generate a full range of locomotion patterns of a legged animal with controlled transitions between different rhythmic patterns. It is shown that gait pattern transition can be achieved by simply changing a single parameter of an OBB module. Essentially this simple mechanism allows for the consolidation of a methodology for the construction of artificial CPG architectures behaving as an asymmetric Hopfield neural network. Moreover, the proposed CPG model introduced here is amenable to analogue and/or digital circuit integration

Experimental and theoretical study of demagnetization fields in superconducting samples of orthorhombic shape

In this work we present a model for the calculation of the magnetic properties of superconductors of orthorhombic shape in the perfect shielding state when an external uniform magnetic field is applied in the direction of one of the principal axes of the sample. Our model accounts for demagnetization effects and it is free of fitting parameters and boundary value conditions. We consider planar linear circuits that lie perpendicular to the direction of the applied field. Calculation of the value of the currents is based on magnetic energy minimization. The model is proved to be accurate enough to reproduce experimental results as long as the dimension along the applied field is not much lower than the other dimensions. Calculations of surface currents, as well as measurements and calculations of magnetization and initial susceptibility, are reported. We also present an empirical formula that provides a good fit to the initial susceptibility of a general sample of orthorhombic shape. Demagnetization effects observed on the experimental results are explained in terms of the induced currents in the superconductor. (C) 2004 American Institute of Physics.96148649

Exploring nucleo-cytoplasmic large DNA viruses in Tara Oceans microbial metagenomes

Nucleo-cytoplasmic large DNA viruses (NCLDVs) constitute a group of eukaryotic viruses that can have crucial ecological roles in the sea by accelerating the turnover of their unicellular hosts or by causing diseases in animals. To better characterize the diversity, abundance and biogeography of marine NCLDVs, we analyzed 17 metagenomes derived from microbial samples (0.2–1.6 μm size range) collected during the Tara Oceans Expedition. The sample set includes ecosystems under-represented in previous studies, such as the Arabian Sea oxygen minimum zone (OMZ) and Indian Ocean lagoons. By combining computationally derived relative abundance and direct prokaryote cell counts, the abundance of NCLDVs was found to be in the order of 104–105 genomes ml−1 for the samples from the photic zone and 102–103 genomes ml−1 for the OMZ. The Megaviridae and Phycodnaviridae dominated the NCLDV populations in the metagenomes, although most of the reads classified in these families showed large divergence from known viral genomes. Our taxon co-occurrence analysis revealed a potential association between viruses of the Megaviridae family and eukaryotes related to oomycetes. In support of this predicted association, we identified six cases of lateral gene transfer between Megaviridae and oomycetes. Our results suggest that marine NCLDVs probably outnumber eukaryotic organisms in the photic layer (per given water mass) and that metagenomic sequence analyses promise to shed new light on the biodiversity of marine viruses and their interactions with potential hosts

Angular dependence of the bulk nucleation field Hc2 of aligned MgB2 crystallites

The angular dependence of the bulk nucleation field of a sample made of aligned MgB2 crystallites was obtained using dc magnetization and ac susceptibility measurements. A good fitting of the data by the three-dimensional anisotropic Ginzburg-Landau theory attests to the bulk nature of the critical field H-c2. We found a mass anisotropy ratio epsilon2 approximate to0.39 that implies an anisotropy of the Fermi velocity, with a ratio of 1.6 between the in-plane and perpendicular directions, if an isotropic gap energy is assumed. For an s-wave anisotropic gap this ratio could increase to 2.5. Besides the fundamental implications of this result, it also implies the use of texturization techniques to optimize the critical current in wires and other polycrystalline forms of MgB2.641

Cerebellar Transcranial Direct Current Stimulation Applied over Multiple Days Does Not Enhance Motor Learning of a Complex Overhand Throwing Task in Young Adults

Cerebellar transcranial direct current stimulation (tDCS) enhances motor skill and learning in relatively simple motor tasks, but it is unclear if c-tDCS can improve motor performance in complex motor tasks. The purpose of this study was to determine the influence of c-tDCS applied over multiple days on motor learning in a complex overhand throwing task. In a double-blind, randomized, between-subjects, SHAM-controlled, experimental design, 30 young adults were assigned to either a c-tDCS or a SHAM group. Participants completed three identical experiments on consecutive days that involved overhand throwing in a pre-test block, five practice blocks with concurrent c-tDCS, and a post-test block. Overhand throwing endpoint accuracy was quantified as the endpoint error. The first dorsal interosseous muscle motor evoked potential (MEP) amplitude elicited by transcranial magnetic stimulation was used to quantify primary motor cortex (M1) excitability modulations via c-tDCS. Endpoint error significantly decreased over the 3 days of practice, but the magnitude of decrease was not significantly different between the c-tDCS and SHAM group. Similarly, MEP amplitude slightly increased from the pre-tests to the post-tests, but these increases did not differ between groups. These results indicate that multi-day c-tDCS does not improve motor learning in an overhand throwing task or increase M1 excitability

Transcranial direct current stimulation of primary motor cortex over multiple days improves motor learning of a complex overhand throwing task

Transcranial direct current stimulation (tDCS) applied to the primary motor cortex (M1) improves motor learning in relatively simple motor tasks performed with the hand and arm. However, it is unknown if tDCS can improve motor learning in complex motor tasks involving whole-body coordination with significant endpoint accuracy requirements. The primary purpose was to determine the influence of tDCS on motor learning over multiple days in a complex over-hand throwing task. This study utilized a double-blind, randomized, SHAM-controlled, between-subjects experimental design. Forty-six young adults were allocated to either a tDCS group or a SHAM group and completed three experimental sessions on three consecutive days at the same time of day. Each experimental session was identical and consisted of overhand throwing trials to a target in a pre-test block, five practice blocks performed simultaneously with 20 min of tDCS, and a post-test block. Overhand throwing performance was quantified as the endpoint error. Transcranial magnetic stimulation was used to obtain motor-evoked potentials (MEPs) from the first dorsal interosseus muscle to quantify changes in M1 excitability due to tDCS. Endpoint error significantly decreased over the three days of practice in the tDCS group but not in the SHAM group. MEP amplitude significantly increased in the tDCS group, but the MEP increases were not associated with increases in motor learning. These findings indicate that tDCS applied over multiple days can improve motor learning in a complex motor tasks in healthy young adults

When worlds collide

The UK has a strong tradition of innovative evaluative health care research. There are, however, considerable forces impeding collaboration between clinicians, academics, patients and their advocates and industry. This paper argues that, if the UK is to regain a position at the forefront of clinical research into evaluation of care, some of these forces need to be overcome. Now, with explicit encouragement from funders within the UK's NHS, it is urgent that all parties discover better ways of working together so that more broad and meaningful research can be produced in a timely fashion