Phenolic contents and in vitro antioxidant activity of four commonly consumed nuts in Algeria

This study was carried out to determine the phenolic contents and the antioxidant activity of four nuts with different solvent extract. Total phenolic compounds, flavonoids, and proanthocyanidin were quantified. Antioxidant activity was evaluated by various in vitro tests, including ferric reducing power, phosphomolybdenum method assay, and free radical scavenging activity. The results showed that the total phenolic contents varied between 0.30 g GAE/100 g (peanuts) and 1.65 g GAE/100 g (walnuts); the flavonoid contents varied between 0.17 g QE/100 g (peanuts) and 0.41 g QE/100 g (hazelnut). The phenolic contents of four nut extracts exhibit potent antioxidant activity. Indeed, walnuts were the richest in total phenolic content and demonstrated the highest potential for overall antioxidant capacity using ferric reducing power assay (FRP), phosphomolybdenum method assay, and free radical scavenging activity (FRSA). Phenolic amounts positively correlated with antioxidant activity tested

Use of (Saccharomyces cerevisiae) Mutant in Bioremediation of Some Heavy Metals

The study aimed to use of baker’s yeast Saccharomyces cerevisiae in Bioremediation of some heavy metals and improving its capability by mutation. The dried baker's yeast from Aldnaamaya China Company used in this study. The yeast subjected to serial diagnostic tests to ensure its belongings to S. cerevisiae. To improve the ability of the yeast to remove the metals, it was mutated by Nitrosoguanidine, and among different mutants, it was found that three of them designated as Sc6-1, Sc6-2 and Sc6-3, were more resistant to the antifungal cycloheximide in a concentration of 5 and 10 µg/ ml. These mutants were selected to study their efficiency to remove metals under the specific circumstances of attachment 10 minutes, pH 6, temperature 25○C, the stirring speed 150 rpm/ min, by using an inoculums size 1×106 cells/ ml in a solution containing 1mg/L of from each metal. it was found that the mutant Sc6-2 has gain an excellent efficiency to remove of chromium, nickel, cobalt, cadmium, lead, iron and copper in combined at a rate 72.23%, while the removal efficiency of these elements by the other mutants Sc6-1 and Sc6-3 were 64.67% and 65.37% respectively

Laminoplasty Versus Laminectomy With Fusion for Treating Multilevel Degenerative Cervical Myelopathy

BACKGROUND: Laminectomy with fusion (LF) and laminoplasty (LP) are common posterior decompression procedures used to treat multilevel degenerative cervical myelopathy (DCM). There is debate on their relative efficacy and safety for treatment of DCM. The goal of this study is to examine outcomes and costs of LF and LP procedures for DCM. METHODS: This is a retrospective review of adult patients ( RESULTS: LP cohort (n=76) and LF cohort (n=59) reported no difference in neck pain at baseline, 1, 6, 12, and 24 months postoperatively (p\u3e.05). Patients were successfully weaned off opioids at similar rates (LF: 88%, LP: 86%). Fixed and variable costs respectively with LF cases hospital were higher, 15.7% and 25.7% compared to LP cases (p=.03 and p CONCLUSIONS: When treating multilevel DCM, LP has similar rates of new or increasing axial neck pain compared to LF. LF was associated with greater hospital costs, length of stay, and complications compared to LP. LP may in fact be a less morbid and more cost-effective alternative to LF for patients without cervical deformity

Third Order Effect of Rotation on Stellar Oscillations of a β\beta-Cephei Star

Here the effect of rotation up to third order in the angular velocity of a star on the p, f and g modes is investigated. To do this, the third-order perturbation formalism presented by Soufi et al. (1998) and revised by Karami (2008), was used. I quantify by numerical calculations the effect of rotation on the oscillation frequencies of a uniformly rotating $\beta$-Cephei star with 12 $M_\odot$. For an equatorial velocity of 90 $\rm km s^{-1}$, it is found that the second- and third-order corrections for $(l,m)=(5,-4)$, for instance, are of order of 0.07% of the frequency for radial order $n=-3$ and reaches up to 0.6% for $n=-20$.Comment: 13 pages, 2 figures, 10 table

LIPOSARCOME DU SEIN. A PROPOS D’UN CAS

Liposarcoma of the breast is an unfrequent tumor that can be found in 45-55 year old nwomen, usually with benign clinical and radiological characteristics. We report the case of a 51-year-old patient. Symptomatology began since 1 month by an increase from the left breast with general conservation of the state. The cytoponction evoke the diagnosis of liposarcoma. Four years and half of follow up after mastectomy alone, the evolution is well. No relapse was observed. Beyond the not very frequent character of this pathology, our observation underlines the interest of the early diagnosis of this type of tumour, by screening of breast cancers without mammography among menopausal women.Le liposarcome du sein est une lésion rare, pouvant toucher la femme de 45 à 55 ans en moyenne et qui présente les caractéristiques cliniques et radiologiques d'une lésion bénigne. Nous rapportons ici le cas d'une patiente de 51 ans ménopausée. La symptomatologie débutait depuis un  mois par une augmentation du sein gauche sans signes associés avec conservation de l’état général. La ponction biopsie anatomopathologique a permis d’évoquer le diagnostic de liposarcome du sein. 4 ans et demi de recul après mastectomie seule, l'évolution est satisfaisante. Au-delà du caractère peu fréquent de cette pathologie, notre observation souligne l’intérêt du  diagnostic précoce de ce type de tumeur, grâce aux mammographies systématiques annuelles chez les femmes ménopausées

Asteroseismology and Interferometry

Asteroseismology provides us with a unique opportunity to improve our understanding of stellar structure and evolution. Recent developments, including the first systematic studies of solar-like pulsators, have boosted the impact of this field of research within Astrophysics and have led to a significant increase in the size of the research community. In the present paper we start by reviewing the basic observational and theoretical properties of classical and solar-like pulsators and present results from some of the most recent and outstanding studies of these stars. We centre our review on those classes of pulsators for which interferometric studies are expected to provide a significant input. We discuss current limitations to asteroseismic studies, including difficulties in mode identification and in the accurate determination of global parameters of pulsating stars, and, after a brief review of those aspects of interferometry that are most relevant in this context, anticipate how interferometric observations may contribute to overcome these limitations. Moreover, we present results of recent pilot studies of pulsating stars involving both asteroseismic and interferometric constraints and look into the future, summarizing ongoing efforts concerning the development of future instruments and satellite missions which are expected to have an impact in this field of research.Comment: Version as published in The Astronomy and Astrophysics Review, Volume 14, Issue 3-4, pp. 217-36

Cell-selective labeling using amino acid precursors for proteomic studies of multicellular environments.

We report a technique to selectively and continuously label the proteomes of individual cell types in coculture, named cell type-specific labeling using amino acid precursors (CTAP). Through transgenic expression of exogenous amino acid biosynthesis enzymes, vertebrate cells overcome their dependence on supplemented essential amino acids and can be selectively labeled through metabolic incorporation of amino acids produced from heavy isotope-labeled precursors. When testing CTAP in several human and mouse cell lines, we could differentially label the proteomes of distinct cell populations in coculture and determine the relative expression of proteins by quantitative mass spectrometry. In addition, using CTAP we identified the cell of origin of extracellular proteins secreted from cells in coculture. We believe that this method, which allows linking of proteins to their cell source, will be useful in studies of cell-cell communication and potentially for discovery of biomarkers

Nonsense-mediated mRNA decay controls the changes in yeast ribosomal protein pre-mRNAs levels upon osmotic stress

The expression of ribosomal protein (RP) genes requires a substantial part of cellular transcription, processing and translation resources. Thus, the RP expression must be tightly regulated in response to conditions that compromise cell survival. In Saccharomyces cerevisiae cells, regulation of the RP gene expression at the transcriptional, mature mRNA stability and translational levels during the response to osmotic stress has been reported. Reprogramming global protein synthesis upon osmotic shock includes the movement of ribosomes from RP transcripts to stress-induced mRNAs. Using tiling arrays, we show that osmotic stress yields a drop in the levels of RP pre-mRNAs in S. cerevisiae cells. An analysis of the tiling array data, together with transcription rates data, shows a poor correlation, indicating that the drop in the RP pre-mRNA levels is not merely a result of the lowered RP transcription rates. A kinetic study using quantitative RT-PCR confirmed the decrease in the levels of several RP-unspliced transcripts during the first 15 minutes of osmotic stress, which seems independent of MAP kinase Hog1. Moreover, we found that the mutations in the components of the nonsense-mediated mRNA decay (NMD), Upf1, Upf2, Upf3 or in exonuclease Xrn1, eliminate the osmotic stress-induced drop in RP pre-mRNAs. Altogether, our results indicate that the degradation of yeast RP unspliced transcripts by NMD increases during osmotic stress, and suggest that this might be another mechanism to control RP synthesis during the stress response