Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells.

Many therapies using mesenchymal stem cells (MSC) rely on their ability to produce and release paracrine signals with chemotactic and pro-angiogenic activity. These characteristics, however, are mostly studied under standard in vitro culture conditions. In contrast, various novel cell-based therapies imply pre-seeding MSC into bio-artificial scaffolds. Here we describe human bone marrow-derived MSC seeded in Integra matrices, a common type of scaffold for dermal regeneration (SDR). We show and measured the distribution of MSC within the SDR, where cells clearly establish physical interactions with the scaffold, exhibiting constant metabolic activity for at least 15 days. In the SDR, MSC secrete VEGF and SDF-1α and induce transwell migration of CD34(+) hematopoietic/endothelial progenitor cells, which is inhibited in the presence of a CXCR4/SDF-1α antagonist. MSC in SDR respond to hypoxia by altering levels of angiogenic signals such as Angiogenin, Serpin-1, uPA, and IL-8. Finally, we show that MSC-containing SDR that have been pre-incubated in hypoxia show higher infiltration of endothelial cells after implantation into immune deficient mice. Our data show that MSC are fully functional ex vivo when implanted into SDR. In addition, our results strongly support the notion of hypoxic pre-conditioning MSC-containing SDR, in order to promote angiogenesis in the wounds

A cool starspot or a second transiting planet in the TrES-1 system?

We investigate the origin of a flux increase found during a transit of TrES-1, observed with the HST. This feature in the HST light curve cannot be attributed to noise and is supposedly a dark area on the stellar surface of the host star eclipsed by TrES-1 during its transit. We investigate the likeliness of two possible hypothesis for its origin: A starspot or a second transiting planet. We made use of several transit observations of TrES-1 from space with the HST and from ground with the IAC-80 telescope. On the basis of these observations we did a statistical study of flux variations in each of the observed events, to investigate if similar flux increases are present in other parts of the data set. The HST observation presents a single clear flux rise during a transit whereas the ground observations led to the detection of two such events but with low significance. In the case of having observed a starspot in the HST data, assuming a central impact between the spot and TrES-1, we would obtain a lower limit for the spot radius of 42000 km. For this radius the spot temperature would be 4690 K, 560 K lower then the stellar surface of 5250 K. For a putative second transiting planet we can set a lower limit for its radius at 0.37 R$_J$ and for periods of less than 10.5 days, we can set an upper limit at 0.72 R$_J$. Assuming a conventional interpretation, then this HST observation constitutes the detection of a starspot. Alternatively, this flux rise might also be caused by an additional transiting planet. The true nature of the origin can be revealed if a wavelength dependency of the flux rise can be shown or discarded with a higher certainty. Additionally, the presence of a second planet can also be detected by radial velocity measurements.Comment: 8 pages, 6 figures, accepted for publication in A&

The Enterovirus Theory of Disease Etiology in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: A Critical Review

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex, multi-system disease whose etiological basis has not been established. Enteroviruses (EVs) as a cause of ME/CFS have sometimes been proposed, as they are known agents of acute respiratory and gastrointestinal infections that may persist in secondary infection sites, including the central nervous system, muscle, and heart. To date, the body of research that has investigated enterovirus infections in relation to ME/CFS supports an increased prevalence of chronic or persistent enteroviral infections in ME/CFS patient cohorts than in healthy individuals. Nevertheless, inconsistent results have fueled a decline in related studies over the past two decades. This review covers the aspects of ME/CFS pathophysiology that are consistent with a chronic enterovirus infection and critically reviews methodologies and approaches used in past EV-related ME/CFS studies. We describe the prior sample types that were interrogated, the methods used and the limitations to the approaches that were chosen. We conclude that there is considerable evidence that prior outbreaks of ME/CFS were caused by one or more enterovirus groups. Furthermore, we find that the methods used in prior studies were inadequate to rule out the presence of chronic enteroviral infections in individuals with ME/CFS. Given the possibility that such infections could be contributing to morbidity and preventing recovery, further studies of appropriate biological samples with the latest molecular methods are urgently needed

Is it possible to detect planets around young active G and K dwarfs?

Theoretical predictions suggest that the distribution of planets in very young stars could be very different to that typically observed in Gyr old systems that are the current focus of radial velocity surveys. However, the detection of planets around young stars is hampered by the increased stellar activity associated with young stars, the signatures of which can bias the detection of planets. In this paper, we place realistic limitations on the possibilities for detecting planets around young active G and K dwarfs. The models of stellar activity based on tomographic imaging of the G dwarf HD 141943 and the K1 dwarf AB Dor also include contributions from plage and many small random starspots. Our results show that the increased stellar activity levels present on young solar-type stars strongly impacts the detection of Earth-mass and Jupiter-mass planets and that the degree of activity jitter is directly correlated with stellar v sin i. We also show that for G and K dwarfs, the distribution of activity in individual stars is more important than the differences in induced radial velocities as a function of spectral type. We conclude that Jupiter-mass planets can be detected close-in around fast-rotating young active stars, Neptune-mass planets around moderate rotators and that Super-Earths are only detectable around very slowly rotating stars. The effects of an increase in stellar activity jitter by observing younger stars can be compensated for by extending the observational base-line to at least 100 epochs.Peer reviewe

Development of a Texas Building Registry

The Texas Building Registry (TBR or Registry) provides data collection, storage, and analysis of above-code residential housing construction in Texas. The original system was developed to support the Texas Emissions Reduction Plan (TERP). Since 2005, the Energy Systems Laboratory (The Laboratory) has provided web-based, above code energy efficiency calculators for public use. The Registry can accept single entry and batch loading of data describing Energy Star™ homes, low-income weatherization programs, or data from municipal code enforcement systems. The Registry is independent of, and respectful of, other rating systems used by partners, thus it can accept data for homes measured against Energy Star, IECC 2000/2001, 2006, 2009, and specially amended Codes. Reports can be given summarized geographically against weather zones, county or cities, and even displayed on a map (i.e., thematic mapping). Additional reports on building characteristics can be analyzed and reported such as: HVAC tonnage per sq. ft; or average SHGC. Data provided by partners is kept secure database server located at the Texas A&M University. Individual results from the Registry will not be displayed to further respect the privacy of individuals who have provided the data.Energy Systems Laboratory, Texas A&M Universit

Expression of Extracellular Matrix Proteins in Human Periodontal Ligament Cells During Mineralization In Vitro

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142249/1/jper0320.pd

Shiva target alignment and viewing instrument

To view and align Shiva laser targets, two new telemicroscopic instruments integral with TV camera and HeNe laser illuminator have been designed. The common requirement of both instruments is the capability of imaging two objects of different sizes on a TV screen: the large surrogate target (5 mm diameter) and the laser fusion target (0.250 mm diameter) with the same resolution (better than 7 ..mu..m). Both instruments have an optical relay which images the targets on a fixed reference reticle; the object is to center each target on the reticle. One of the instruments reimages the reticle plane onto the TV dectector using a zoom arrangement. This instrument translates the TV camera-zoom assembly in three axes and is thereby capable of exploring an object-space volume of 1 cm/sup 3/. In the other instrument, the reticle plane is reimaged by a zoom lens and this enlarged image is relayed to the TV detector by a cluster of five lenses. Four lateral lenses image the periphery of the surrogate target and the reticle for coincidence. The central objective images the center of the reticle and the fusion target when it is centered

Genome scan of Diabrotica virgifera virgifera for genetic variation associated with crop rotation tolerance

Crop rotation has been a valuable technique for control of Diabrotica virgifera virgifera for almost a century. However, during the last two decades, crop rotation has ceased to be effective in an expanding area of the US corn belt. This failure appears to be due to a change in the insect's oviposition behaviour, which, in all probability, has an underlying genetic basis. A preliminary genome scan using 253 amplified fragment-length polymorphism (AFLP) markers sought to identify genetic variation associated with the circumvention of crop rotation. Samples of D. v. virgifera from east-central Illinois, where crop rotation is ineffective, were compared with samples from Iowa at locations that the behavioural variant has yet to reach. A single AFLP marker showed signs of having been influenced by selection for the circumvention of crop rotation. However, this marker was not diagnostic. The lack of markers strongly associated with the trait may be due to an insufficient density of marker coverage throughout the genome. A weak but significant general heterogeneity was observed between the Illinois and Iowa samples at microsatellite loci and AFLP markers. This has not been detected in previous population genetic studies of D. v. virgifera and may indicate a reduction in gene flow between variant and wild-type beetles