Starch accumulation and agronomical performance of Syrah under winter cycle: responses to pruning and ethephon management

In the Brazilian Southeast, 'Syrah' grape harvested during the winter reaches better quality index than those from summer harvest. However, the management of annual double pruning to produce two grapevine growth cycles (vegetative cycle: spring – summer; reproductive cycle: autumn – winter) has increased the vineyard production costs and the vine reserve accumulation can be compromised. This study had two main objectives to improve the winter cultivation: i) to validate single pruning carried out only in summer (February) (experiment 1); to increase the reserve accumulation by ethephon (ethrel) sprayed two months before yield pruning (experiment 2). Both experiments were carried out in south of Minas Gerais State using three years old field-grown 'Syrah' grapevines grafted onto '1103 Paulsen' and trained in a vertical shoot position. The results from experiment 1 showed that grapevines pruned in summer also need to be pruned during the winter after grape harvest to avoid bud infertility during the following autumn-winter season. Single pruning reduced the starch contents in shoots, trunks and roots sampled before yield pruning and the commercial grape harvest was completely unavailable due to unfruitful shoots. On the other hand, the autumn-winter cycle was improved by ethephon sprayed in the previous vegetative growing cycle (summer cycle). During the autumn-winter cycle, sprayed grapevines showed higher starch content in trunks, high vegetative vigor and the yield and grape quality were not negatively affected

Effect of pruning strategy on 'Syrah' bud necrosis and fruitfulness in Brazilian subtropical Southeast

The change of wine grape harvest from wet season (summer) to dry season (winter) by changing the pruning management has improved quality of wines produced in the Brazilian Southeast. However, the vines need to be spur pruned twice a year, i.e. with a 1st pruning in August (winter pruning) for a vegetative cycle during the hot and wet summer, and a 2nd pruning in January (summer pruning) for a productive cycle during the cold and dry season. This double pruning strategy is made necessary by the fact that latent buds developed during the dry season cycle are not fruitful to support a productive cycle in the following year. This histological study, performed in the South of Minas Gerais State (Brazil), showed that annual single pruning done in the wet season (in January) displayed a high rate of necrosis on primary and secondary buds (bud necrosis – BN). In April, 99 days after summer pruning (DASP), the rates of BN were 40 % and 50 % at basal and apical node positions, respectively, reaching 80 % of BN in December (322 DASP). As a consequence of BN, bud potential fertility was drastically reduced from 0.5 inflorescence primordial (IP) per bud (in July) to 0.06 (in December) and bud burst in the next cycle from secondary and tertiary bud axes. Vines managed by double pruning system (submitted to summer and winter pruning) displayed a much higher fruitfulness potential, i.e. 1.46 IP per bud in December (112 days after winter pruning) and limited BN occurrence (20 %). On single pruned vines, we also observed a significant decrease of starch content in canes, trunks and roots. Internal bud anatomy showed that a random cell breakdown started 70 days DASP. At 211 DASP, all buds showed a large starch granule concentration, raphides and crystals of calcium oxalate inside idioblasts of leaf primordia and also in cortical parenchyma of the vegetative axis. The bud starch content was increased and a positive correlation between necrosis and starch accumulation was observed. The impact of carbohydrate availability on bud necrosis development was discussed. This study showed that the necrosis development towards secondary and tertiary axis of the dry season buds is the main reason of unfruitfulness in the vineyards managed by single pruning in the wet season, making the double pruning compulsory

Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries.

BACKGROUND: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. METHODS: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. RESULTS: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44 814 patients with a median hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. CONCLUSIONS: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. STUDY REGISTRATION: ISRCTN5181700

Conhecimento tradicional das marisqueiras de Barra Grande, área de proteção ambiental do delta do Rio Parnaíba, Piauí, Brasil

Na comunidade de Barra Grande localizada no litoral do Piauí, as mulheres dos pescadores, conhecidas localmente como marisqueiras, extraem do manguezal vários tipos de moluscos para comercialização e em maior escala para a subsistência. Dados sobre a atividade de mariscagem, conceitos de conservação e aspectos socioeconômicos do contexto em que ocorre a atividade de cata dos moluscos, foram revelados após a aplicação de protocolos estruturados e semiestruturados. O molusco bivalve Anomalocardia brasiliana (Gmelin, 1791), popularmente denominado de marisco, é caracterizado por ser um importante recurso para a subsistência, sendo o mais coletado pela comunidade, seguido da Mytella charruana (d'Orbigny, 1842), o sururu, explorado para a comercialização por ter o maior valor de mercado. O conhecimento das marisqueiras sobre A. brasiliana foi comparado com literatura especializada, muitas vezes mostrando-se em harmonia com esta. Demonstra-se assim que o conhecimento tradicional deve ser valorizado para delineamento de programas de gestão de recursos pesqueiros da região. Quanto aos modos de pensar, foi revelado que 82,81% das marisqueiras praticam manejo para a conservação da A. brasiliana ao coletar apenas os indivíduos de maior tamanho, e 80,86% consideram que não existe poluição nos pontos de coletas. O surgimento de uma Associação exclusiva e cooperativa para as marisqueiras é necessário para a valorização do trabalho por elas desenvolvido, visto que há uma média estimada de 351 kg de carne sendo extraídos mensalmente.<br>En la comunidad de Barra Grande, situada en la costa del estado de Piaui, las esposas de los pescadores, localmente llamadas de "marisqueiras", extraen de los manglares diversos tipos de moluscos para su comercialización, haciendo de esta actividad en una fuente de subsistencia. Informaciones detalladas y sistemáticas sobre la actividad de cosecha de mariscos, del contexto en el que se desarrolla la recolección y sus aspectos socioeconómicos, asi como la percepción ambiental y los conceptos relacionados a la preservación que tienen las "marisqueiras", sólo fueron conocidas luego de la aplicación de protocolos estructurados y semi-estructurados. El molusco Anomalocardia brasiliana (Gmelin, 1791), popularmente conocido como "marisco", es una importante fuente de recursos, siendo el más cosechado por la comunidad; le sigue en importancia el Mytella charruana (d'Orbigny, 1842), conocido como "sururu", cuya explotación se destina principalmente a la comercialización debido a su superior valor de mercado. El conocimiento que las "marisqueiras" tienen de la A. brasiliana fue comparado con el disponible en la literatura especializada, quedando evidente su compatibilidad, lo cual fundamenta la importancia de su valorización en el diseño de programas de gestión de los recursos pesqueros en la región. Estudios sobre el impacto ambiental de la actividad revelaron que el 82,81% de las "marisqueiras" tienen en cuenta las consecuencias de su actividad sobre la preservación, cosechando sólo los ejemplares más grandes, mientras que 80,86% de las trabajadoras considera que no hay contaminación en las zonas de cosecha. En el contexto actual se hace necesaria la creación de una Asociación de "marisqueiras", así como una cooperativa, con el propósito de jerarquizar la actividad, la cual es responsable por la producción estimada en promedio de 351 kg de carne por mes.<br>In the community of Barra Grande located at the coast of Piauí, the wives of fishermen, locally known as "marisqueiras" (female shellfish catcher), extract from the mangrove various kinds of shellfish for commercial purposes and a great amount for subsistence. For a better understanding, data on the activity of "marisqueiras", concepts of conservation and socioeconomic aspects of the context in which occurs the activity of shellfish catching were revealed after the application of semi-structured and structured protocols. The bivalve mussel Anomalocardia brasiliana (Gmelin, 1791), popularly known as "marisco", is characterized as an important resource for subsistence being most of it collected by the community, followed by Mytella charruana (d'Orbigny, 1842) the "sururu", extract for commercial purposes due to its highest market value. The knowledge of the "marisqueiras" about A. brasiliana was compared with literature which proved to be compatible, thus showing that it should be valued for the design of programs for the management of fishing resources in the region. About the way of thinking, it was revealed that 82.81% of "marisqueiras" practice the conservation of the A. brasiliana by collecting only the larger individuals and 80.86% consider that there is no pollution at the collection points. The creation of a unique association and a cooperative for the "marisqueiras" is required to value the work by themselves developed, since there are an estimated average of 351 kg of meat being extracted monthly