Characterization of Bacteriophages of Pseudomonas syringae pv. tomato

Bacteriophages from supernatants of the plant pathogenic bacteria Pseudomonas syringae pv. tomato (P. tomato) were isolated, enriched, and purified by density block centrifugation in cesium chloride (CsCl) step gradients. The DNA from purified phage was isolated and digested with the restriction endonucleases EcoRl or HindIII. Three different DNA fingerprint patterns were determined indicating 3 unique phage isolates. Genome sizes of the phage ranged from 40 to 52 kilobases (kB). Buoyant densities of phage particles in CsCI varied from 1.36 to 1.51 glml. Electron microscopy revealed a single morphological type with an elongated polyhedral head and a long tail indicating the family Siphovirida

Corrections for pumped SBE 41CP CTDs determined from stratified tank experiments

Author Posting. © American Meteorological Society, 2019. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 36(4), (2019): 733-744, doi:10.1175/JTECH-D-18-0050.1.Sea-Bird Scientific SBE 41CP CTDs are used on autonomous floats in the global Argo ocean observing program to measure the temperature and salinity of the upper ocean. While profiling, the sensors are subject to dynamic errors as they profile through vertical gradients. Applying dynamic corrections to the temperature and conductivity data reduces these errors and improves sensor accuracy. A series of laboratory experiments conducted in a stratified tank are used to characterize dynamic errors and determine corrections. The corrections are adapted for Argo floats, and recommendations for future implementation are presented.2020-04-2

Reply to "comments on 'corrections for pumped SBE 41CP CTDs determined from stratified tank experiments'"

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Martini, K. I., Murphy, D. J., Schmitt, R. W., & Larson, N. G. Reply to "comments on 'corrections for pumped SBE 41CP CTDs determined from stratified tank experiments'". Journal of Atmospheric and Oceanic Technology, 37(2), (2020): 357-363, doi:10.1175/JTECH-D-19-0171.1.The response in Johnson (2020) that the method used to determine cell thermal mass correction coefficients for SBE 41CP CTD data from Argo floats is biased as determined by Martini et al. (2019) is valid. However, the recommendation for correction coefficients should not be followed due to these three errors in Johnson (2020): Alignment is as large a source of dynamic error as cell thermal mass in the SBE 41CP CTD. Order of operations was overlooked, so that cell thermal mass is used to correct for alignment errors caused by the temporal mismatch of temperature and conductivity. The cell thermal mass corrections determined in Johnson et al. (2007) and Johnson (2020) also bias salinity. In this response we will do the following: Detail how the corrections in Johnson (2020) are biased because the optimization procedure does not accurately model physics in the tank and conductivity cell. Verify using in situ data from Argo floats deployed in the ocean that alignment is a significant source of error for the SBE 41CP as shown in Martini et al. (2019). Determine cell thermal mass correction coefficients from the stratified tank experiment merging the methods of Johnson (2020) and Martini et al. (2019) to optimize against a model that better represents the physics in the tank and conductivity cell. Compare the corrections using in situ data using the coefficients determined in Johnson et al. (2007), Martini et al. (2019), Johnson (2020), and this manuscript.Thanks to Pelle Robbins for finding the in situ profiles used for this analysis in the vast database of Argo floats, John Gilson showing me how to access that high-resolution data, Ray Schmitt for use of the stratified tank, Susan Wijffels, Breck Owens, and Annie Wong for intellectual support, and Diego Sorrentino and Vlad Simontov for validating the sampling scheme in the SBE 41CP.2020-08-2

Comparison of TWP-ICE Satellite and Field Campaign Aircraft Derived Cloud Properties

Cloud and radiation products derived from the MTSAT-1R satellite have been developed for TWP-ICE. These include pixel-level, gridded, and ground site and aircraft matched. These products are available from the Langley website and the ARM data center. As shown in Figs 2, and 4-6, these products compare favorably with in-situ ground and aircraft based measurements. With additional quantitative validation these products can provide valuable information about tropical convection and its impact on the radiation budget and climate. As new algorithm improvements, such as multi-layer cloud detection, are implemented these products will be reprocessed and updated

Tropospheric Airborne Meteorological Data and Reporting (TAMDAR) Icing Sensor Performance during the 2003/2004 Alliance Icing Research Study (AIRS II)

NASA Langley Research Center and its research partners from the University of North Dakota (UND) and the National Center for Atmospheric Research (NCAR) participated in the AIRS II campaign from November 17 to December 17, 2003. AIRS II provided the opportunity to compare TAMDAR in situ in-flight icing condition assessments with in situ data from the UND Citation II aircraft's Rosemont system. TAMDAR is designed to provide a general warning of ice accretion and to report it directly into the Meteorological Data Communications and Reporting System (MDCRS). In addition to evaluating TAMDAR with microphysical data obtained by the Citation II, this study also compares these data to the NWS operational in-flight icing Current Icing Potential (CIP) graphic product and with the NASA Advanced Satellite Aviation-weather Products (ASAP) Icing Severity product. The CIP and ASAP graphics are also examined in this study to provide a context for the Citation II's sorties in AIRS II

The effect of muscle-tendon unit vs. fascicle analyses on vastus lateralis force-generating capacity during constant power output cycling with variable cadence

The maximum force-generating capacity of a muscle is dependent on the lengths and velocities of its contractile apparatus. Muscle-tendon unit (MTU) length changes can be estimated from joint kinematics; however, contractile element length changes are more difficult to predict during dynamic contractions. The aim of this study was to compare vastus lateralis (VL) MTU and fascicle level force-length and force-velocity relationships, and dynamic muscle function while cycling at a constant submaximal power output (2.5 W/kg) with different cadences. We hypothesized that manipulating cadence at a constant power output would not affect VL MTU shortening, but significantly affect VL fascicle shortening. Furthermore, these differences would affect the predicted force capacity of the muscle. Using an isokinetic dynamometer and B-mode ultrasound (US), we determined the force-length and force-velocity properties of the VL MTU and its fascicles. In addition, three-dimensional kinematics and kinetics of the lower limb, as well as US images of VL fascicles were collected during submaximal cycling at cadences of 40, 60, 80, and 100 rotations per minute. Ultrasound measures revealed a significant increase in fascicle shortening as cadence decreased (84% increase across all conditions, P < 0.01), whereas there were no significant differences in MTU lengths across any of the cycling conditions (maximum of 6%). The MTU analysis resulted in greater predicted force capacity across all conditions relative to the force-velocity relationship (P < 0.01). These results reinforce the need to determine muscle mechanics in terms of separate contractile element and connective tissue length changes during isokinetic contractions, as well as dynamic movements like cycling

8-Alkylthio-6-thio-substituted theophylline analogues as selective noncompetitive progesterone receptor antagonists

The progesterone receptor (PR) plays a key role in reproduction and is important in cancers of the reproductive tract. Current PR antagonists usually compete for progestin binding in the PR ligand-binding pocket and often exhibit cross-binding with other members of the steroid receptor family. Using stably transfected cells expressing reporter genes, a set of ~150 theophylline analogues were screened for their ability to inhibit progesterone, estrogen, glucocorticoid and androgen signaling. The structure-activity studies presented here identify branched 8-alkylthio-6-thio-substitutions of theophylline as selective PR inhibitors. 6-thio-8-(2-ethylbutyl)thiotheophylline (51), the most extensively studied derivative, does not act by competing with progestins for binding in the ligand-binding pocket of PR. It demonstrated the ability to inhibit the mouse mammary tumor virus (MMTV)-luciferase reporter and endogenous PR-regulated alkaline phosphatase activity in T47D breast cancer cells. Compound 51 is the lead member of a novel class of PR inhibitors that act outside the PR ligand-binding pocket, thus serving as a novel probe to investigate PR action and a lead for further development

Concentration-dependent exchange accelerates turnover of proteins bound to double-stranded DNA

The multistep kinetics through which DNA-binding proteins bind their targets are heavily studied, but relatively little attention has been paid to proteins leaving the double helix. Using single-DNA stretching and fluorescence detection, we find that sequence-neutral DNA-binding proteins Fis, HU and NHP6A readily exchange with themselves and with each other. In experiments focused on the Escherichia coli nucleoid-associated protein Fis, only a small fraction of protein bound to DNA spontaneously dissociates into protein-free solution. However, if Fis is present in solution, we find that a concentration-dependent exchange reaction occurs which turns over the bound protein, with a rate of kexch = 6 × 104 M−1s−1. The bacterial DNA-binding protein HU and the yeast HMGB protein NHP6A display the same phenomenon of protein in solution accelerating dissociation of previously bound labeled proteins as exchange occurs. Thus, solvated proteins can play a key role in facilitating removal and renewal of proteins bound to the double helix, an effect that likely plays a major role in promoting the turnover of proteins bound to DNA in vivo and, therefore, in controlling the dynamics of gene regulation

Kinases and protein phosphorylation as regulators of steroid hormone action

Although the primary signal for the activation of steroid hormone receptors is binding of hormone, there is increasing evidence that the activities of cell signaling pathways and the phosphorylation status of these transcription factors and their coregulators determine the overall response to the hormone. In some cases, enhanced cell signaling is sufficient to cause activation of receptors in medium depleted of steroids. Steroid receptors are targets for multiple kinases. Many of the phosphorylation sites contain Ser/Thr-Pro motifs implicating proline-directed kinases such as the cyclin-dependent kinases and the mitogen-activated kinases (MAPK) in receptor phosphorylation. Although some sites are constitutively phosphorylated, others are phosphorylated in response to hormone. Still others are only phosphorylated in response to specific cell signaling pathways. Phosphorylation of specific sites has been implicated not only in overall transcriptional activity, but also in nuclear localization, protein stability, and DNA binding. The studies of the roles of phosphorylation in coregulator function are more limited, but it is now well established that many of them are highly phosphorylated and that phosphorylation regulates their function. There is good evidence that some of the phosphorylation sites in the receptors and coregulators are targets of multiple signaling pathways. Individual sites have been associated both with functions that enhance the activity of the receptor, as well as with functions that inhibit activity. Thus, the specific combinations of phosphorylations of the steroid receptor combined with the expression levels and phosphorylation status of coregulators will determine the genes regulated and the biological response