Thermal Equilibria of Optically Thin, Magnetically Supported, Two-Temperature, Black Hole Accretion Disks

We obtained thermal equilibrium solutions for optically thin, two-temperature black hole accretion disks incorporating magnetic fields. The main objective of this study is to explain the bright/hard state observed during the bright/slow transition of galactic black hole candidates. We assume that the energy transfer from ions to electrons occurs via Coulomb collisions. Bremsstrahlung, synchrotron, and inverse Compton scattering are considered as the radiative cooling processes. In order to complete the set of basic equations, we specify the magnetic flux advection rate. We find magnetically supported (low-beta), thermally stable solutions. In these solutions, the total amount of the heating via the dissipation of turbulent magnetic fields goes into electrons and balances the radiative cooling. The low-$\beta$ solutions extend to high mass accretion rates and the electron temperature is moderately cool. High luminosities and moderately high energy cutoffs in the X-ray spectrum observed in the bright/hard state can be explained by the low-beta solutions.Comment: 24 pages, 10 figures,accepted for publication in Astrophysical Journa

Broad Ranges of Affinity and Specificity of Anti-Histone Antibodies Revealed by a Quantitative Peptide Immunoprecipitation Assay

Antibodies directed against histone posttranslational modifications (PTMs) are critical tools in epigenetics research, particularly in the widely used chromatin immunoprecipitation (ChIP) experiments. However, a lack of quantitative methods for characterizing such antibodies has been a major bottleneck in accurate and reproducible analysis of histone modifications. Here, we report a simple and sensitive method for quantitatively characterizing polyclonal and monoclonal antibodies for histone PTMs in a ChIP-like format. Importantly, it determines the apparent dissociation constants for the interactions of an antibody with peptides harboring cognate or off-target PTMs. Analyses of commercial antibodies revealed large ranges of affinity, specificity and binding capacity as well as substantial lot-to-lot variations, suggesting the importance of quantitatively characterizing each antibody intended to be used in ChIP experiments and optimizing experimental conditions accordingly. Furthermore, using this method we identified additional factors potentially affecting the interpretation of ChIP experiments

Thermal Equilibria of Magnetically Supported, Black Hole Accretion Disks

We present new thermal equilibrium solutions for optically thin and thick disks incorporating magnetic fields. The purpose of this paper is to explain the bright hard state and the bright/slow transition observed in the rising phases of outbursts in BHCs. On the basis of the results of 3D MHD simulations, we assume that magnetic fields inside the disk are turbulent and dominated by the azimuthal component and that the azimuthally averaged Maxwell stress is proportional to the total pressure. We prescribe the magnetic flux advection rate to determine the azimuthal magnetic flux at a given radius. We find magnetically supported, thermally stable solutions for both optically thin and thick disks, in which the heating enhanced by the strong magnetic field balances the radiative cooling. The temperature in a low-$\beta$ disk is lower than that in an ADAF/RIAF but higher than that in a standard disk. We also study the radial dependence of the thermal equilibrium solutions. The optically thin, low-$\beta$ branch extends to $\dot M \gtrsim 0.1 {\dot M}_{\rm Edd}$, in which the temperature anti-correlates with the mass accretion rate. Thus optically thin low-$\beta$ disks can explain the bright hard state. Optically thick, low-$\beta$ disks have the radial dependence of the effective temperature $T_{\rm eff} \propto \varpi^{-3/4}$. Such disks will be observed as staying in a high/soft state. Furthermore, limit cycle oscillations between an optically thick low-$\beta$ disk and a slim disk will occur because the optically thick low-$\beta$ branch intersects with the radiation pressure dominated standard disk branch. These limit cycle oscillations will show a smaller luminosity variation than that between a standard disk and a slim disk.Comment: 23 pages, 9 figures, accepted for publication in Ap

SIMULATED FARADAY ROTATION MEASURES TOWARD HIGH GALACTIC LATITUDES

We study the Faraday rotation measure (RM) due to the Galactic magnetic field (GMF) toward high Galactic latitudes. The RM arises from the global, regular component as well as from the turbulent, random component of the GMF. We model the former based on observations and the latter using the data of magnetohydrodynamic turbulence simulations. For a large number of different GMF models, we produce mock RM maps around the Galactic poles and calculate various statistical quantities with the RM maps. We find that the observed medians of RMs toward the north and south Galactic poles, 0.0 ?? 0.5 rad m -2 and + 6.3 ?? 0.5 rad m-2, are difficult to explain with any of our many alternate GMF models. The standard deviation of observed RMs, 9 rad m-2, is clearly larger than that of simulated RMs. The second-order structure function of observed RMs is substantially larger than that of simulated RMs, especially at small angular scales. We discuss other possible contributions to RM toward high Galactic latitudes. Besides observational errors and the intrinsic RM of background radio sources against which RM is observed, we suggest that the RM due to the intergalactic magnetic field may account for a substantial fraction of the observed RM. Finally, we note that reproducing the observed medians may require additional components or/and structures of the GMF that are not present in our models.open5

P38 Mitogen-Activated Protein Kinase Inhibitor, FR167653, Inhibits Parathyroid Hormone Related Protein-Induced Osteoclastogenesis and Bone Resorption

p38 mitogen-activated protein kinase (MAPK) acts downstream in the signaling pathway that includes receptor activator of NF-κB (RANK), a powerful inducer of osteoclast formation and activation. We investigated the role of p38 MAPK in parathyroid hormone related protein (PTHrP)-induced osteoclastogenesis in vitro and PTHrP-induced bone resorption in vivo. The ability of FR167653 to inhibit osteoclast formation was evaluated by counting the number of tartrate-resistant acid phosphatase positive multinucleated cells (TRAP-positive MNCs) in in vitro osteoclastgenesis assays. Its mechanisms were evaluated by detecting the expression level of c-Fos and nuclear factor of activated T cells c1 (NFATc1) in bone marrow macrophages(BMMs) stimulated with sRANKL and M-CSF, and by detecting the expression level of osteoprotegerin (OPG) and RANKL in bone marrow stromal cells stimulated with PTHrP in the presence of FR167653. The function of FR167653 on bone resorption was assessed by measuring the bone resorption area radiographically and by counting osteoclast number per unit bone tissue area in calvaria in a mouse model of bone resorption by injecting PTHrP subcutaneously onto calvaria. Whole blood ionized calcium levels were also recorded. FR167653 inhibited PTHrP-induced osteoclast formation and PTHrP-induced c-Fos and NFATc1 expression in bone marrow macrophages, but not the expression levels of RANKL and OPG in primary bone marrow stromal cells treated by PTHrP. Furthermore, bone resorption area and osteoclast number in vivo were significantly decreased by the treatment of FR167653. Systemic hypercalcemia was also partially inhibited. Inhibition of p38 MAPK by FR167653 blocks PTHrP-induced osteoclastogenesis in vitro and PTHrP-induced bone resorption in vivo, suggesting that the p38 MAPK signaling pathway plays a fundamental role in PTHrP-induced osteoclastic bone resorption

The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System

Bacteria, fungi, protozoa, chromista and plants all harbor homologues of Hsp104, a AAA+ ATPase that collaborates with Hsp70 and Hsp40 to promote protein disaggregation and reactivation. Curiously, however, metazoa do not possess an Hsp104 homologue. Thus, whether animal cells renature large protein aggregates has long remained unclear. Here, it is established that mammalian cytosol prepared from different sources possesses a potent, ATP-dependent protein disaggregase and reactivation activity, which can be accelerated and stimulated by Hsp104. This activity did not require the AAA+ ATPase, p97. Rather, mammalian Hsp110 (Apg-2), Hsp70 (Hsc70 or Hsp70) and Hsp40 (Hdj1) were necessary and sufficient to slowly dissolve large disordered aggregates and recover natively folded protein. This slow disaggregase activity was conserved to yeast Hsp110 (Sse1), Hsp70 (Ssa1) and Hsp40 (Sis1 or Ydj1). Hsp110 must engage substrate, engage Hsp70, promote nucleotide exchange on Hsp70, and hydrolyze ATP to promote disaggregation of disordered aggregates. Similarly, Hsp70 must engage substrate and Hsp110, and hydrolyze ATP for protein disaggregation. Hsp40 must harbor a functional J domain to promote protein disaggregation, but the J domain alone is insufficient. Optimal disaggregase activity is achieved when the Hsp40 can stimulate the ATPase activity of Hsp110 and Hsp70. Finally, Hsp110, Hsp70 and Hsp40 fail to rapidly remodel amyloid forms of the yeast prion protein, Sup35, or the Parkinson's disease protein, alpha-synuclein. However, Hsp110, Hsp70 and Hsp40 enhanced the activity of Hsp104 against these amyloid substrates. Taken together, these findings suggest that Hsp110 fulfils a subset of Hsp104 activities in mammals. Moreover, they suggest that Hsp104 can collaborate with the mammalian disaggregase machinery to rapidly remodel amyloid conformers

Social Transfer of Pathogenic Fungus Promotes Active Immunisation in Ant Colonies

Social contact with fungus-exposed ants leads to pathogen transfer to healthy nest-mates, causing low-level infections. These micro-infections promote pathogen-specific immune gene expression and protective immunization of nest-mates