Evaluation of systematic review utilization in the development of OB-GYN randomized controlled trials

Introduction: The issue of research waste has been raised due to the fact that 85% of funding for biomedical research has been improperly utilized. A prominent issue is the frequency of randomized controlled trials (RCTs) being conducted without prior consultation of existing support, such as systematic reviews (SRs). Meticulous monitoring is necessary to ensure that clinical recommendations are being made with confidence in high-quality biomedical practices. The aim of this study was to survey Obstetric and Gynecology journals to analyze their published articles for citation of SR for justification of conducting the RCT.Methods: We conducted a search of PubMed for RCTs published between January 1, 2014 and December 31, 2017, in the top ten Obstetric and Gynecology journals. Each included study was evaluated to determine the number of SRs cited within the introduction, methods, and discussion sections. We further analyzed whether the SR was cited verbatim or indirectly, number of participants, type of intervention being studied, funding source, type of trial, and how the outcome was perceived.Results: Of the 720 articles from our initial search, 458 (63.61%) met inclusion criteria. Of the 458 included studies, 279 (60.92%) cited an SR in the introduction, 34 (7.42%) cited an SR in the methods, and 207 (45.2%) cited an SR in the discussion as justification for conducting the study.Conclusion: A large portion of the RCTs being published in clinical Obstetrics and Gynecology journals are not citing SRs as justification for conducting their studies, which may be leading to an increase in research waste

Cannabidiolic acid in Hemp Seed Oil Table Spoon and Beyond

Cannabidiolic acid (CBDA) is the main precannabinoid in industrial hemp. It represents a common constituent of hemp seed oil, but mainly abundant in the aerial parts of the plant (including their processing waste). Thus, the optimization of fast and low-cost purification strategies is mandatory, as well as a deep investigation on its nutraceutical and cosmeceutical properties. To this purpose, CBDA content in hemp seed oil is evaluated, and its recovery from wasted leaves is favorably achieved. The cytotoxicity screening towards HaCaT cells, by means of MTT, SRB and LDH release assays, suggested it was not able to decrease cell viability or perturb cell integrity up to 10 μM concentration. Thus, the ability of CBDA to differentially modulate the release of proinflammatory cytokines and chemokines mediators has been evaluated, finding that CBDA decreased IFN-γ, CXCL8, CXCL10, CCL2, CCL4 and CCL5, mostly in a dose-dependent manner, with 10 μM tested concentration exerting the highest activity. These data, together with those from assessing antimicrobial activity against Gram(+) and Gram(-) bacteria and the antibiofilm formation, suggest that CBDA is able to counteract the inflammatory response, also preventing bacteria colonization

Clinical relevance of gene mutations and rearrangements in advanced differentiated thyroid cancer

Background: Tumor genotyping is becoming crucial to optimize the clinical management of patients with advanced differentiated thyroid cancer (DTC); however, its implementation in clinical practice remains undefined. We herein report our single-center experience on molecular advanced DTC testing by next-generation sequencing approach, to better define how and when tumor genotyping can assist clinical decision making. Materials and methods: We retrospectively collected data on all adult patients with advanced DTC who received molecular profiling at the IRCSS Sant'Orsola-Malpighi Hospital from 2008 to 2022. The genetic alterations were correlated with radioactive iodide refractory (RAI-R), RAI uptake/disease status, and time to RAI resistance (TTRR) development. Results: A significant correlation was found between RAI-R development and genetic alterations (P = 0.0001). About 48.7% of RAI-R cases were positive for TERT/TP53 mutations (as both a single event and comutations with other driver gene alterations, such as BRAF mutations, RAS mutations, or gene fusions), while the great majority of RAI-sensitive cases carried gene fusions (41.9%) or were wild type (WT; 41.9%). RAI uptake/disease status and time to TTRR were significantly associated with genetic alterations (P = 0.0001). In particular, DTC with TERT/TP53 mutations as a single event or as comutations displayed a shorter median TTRR of 35.4 months (range 15.0-55.8 months), in comparison to the other molecular subgroups. TERT/TP53 mutations as a single event or as comutations remained independently associated with RAI-R after Cox multivariate analysis (hazard ratio 4.14, 95% CI 1.51-11.32; P = 0.006). Conclusions: Routine testing for genetic alterations should be included as part of the clinical workup, for identifying both the subset of more aggressive tumors and the subset of tumors harboring actionable gene fusions, thus ensuring the appropriate management for all patients with advanced DTC

Disease Rescue and Increased Lifespan in a Model of Cardiomyopathy and Muscular Dystrophy by Combined AAV Treatments

The BIO14.6 hamster is an excellent animal model for inherited cardiomyopathy, because of its lethal and well-documented course, due to a spontaneous deletion of delta-sarcoglycan gene promoter and first exon. The muscle disease is progressive and average lifespan is 11 months, because heart slowly dilates towards heart failure.Based on the ability of adeno-associated viral (AAV) vectors to transduce heart together with skeletal muscle following systemic administration, we delivered human delta-sarcoglycan cDNA into male BIO14.6 hamsters by testing different ages of injection, routes of administration and AAV serotypes. Body-wide restoration of delta-SG expression was associated with functional reconstitution of the sarcoglycan complex and with significant lowering of centralized nuclei and fibrosis in skeletal muscle. Motor ability and cardiac functions were completely rescued. However, BIO14.6 hamsters having less than 70% of fibers recovering sarcoglycan developed cardiomyopathy, even if the total rescued protein was normal. When we used serotype 2/8 in combination with serotype 2/1, lifespan was extended up to 22 months with sustained heart function improvement.Our data support multiple systemic administrations of AAV as a general therapeutic strategy for clinical trials in cardiomyopathies and muscle disorders

Perspectives for sustainable aviation biofuels in Brazil

The aviation industry has set ambitious goals to reduce carbon emissions in coming decades. The strategy involves the use of sustainable biofuels, aiming to achieve benefits from environmental, social, and economic perspectives. In this context, Brazilian conditions are favorable, with a mature agroindustry that regularly produces automotive biofuel largely adopted by Brazilian road vehicles, while air transportation has been growing at an accelerating pace and a modern aircraft industry is in place. This paper presents the main conclusions and recommendations from a broad assessment of the technological, economic, and sustainability challenges and opportunities associated with the development of drop-in aviation biofuels in Brazil. It was written by a research team that prepared the initial reports and conducted eight workshops with the active participation of more than 30 stakeholders encompassing the private sector, government institutions, NGOs, and academia. The main outcome was a set of guidelines for establishing a new biofuels industry, including recommendations for (a) filling the identified research and development knowledge gaps in the production of sustainable feedstock; (b) overcoming the barriers in conversion technology, including scaling-up issues; (c) promoting greater involvement and interaction between private and government stakeholders; and (d) creating a national strategy to promote the development of aviation biofuels2015FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2012/50009-

Isotope correlations as a probe for freeze-out characterization: central 124Sn+64Ni, 112Sn+58Ni collisions

124Sn+64Ni and 112Sn+58Ni reactions at 35 AMeV incident energy were studied with the forward part of CHIMERA multi-detector. The most central collisions were selected by means of a multidimensional analysis. The characteristics of the source formed in the central collisions, as size, temperature and volume, were inspected. The measured isotopes of light fragments (3 <= Z <=8) were used to examine isotope yield ratios that provide information on the free neutron to proton densities.Comment: 4 pages, Contribution to 8th International Conference on Nucleus-Nucleus Collisions, Moscow 200

Bi-allelic KARS1 pathogenic variants affecting functions of cytosolic and mitochondrial isoforms are associated with a progressive and multisystem disease

KARS1 encodes a lysyl-transfer RNA synthetase (LysRS) that links lysine to its cognate transfer RNA. Two different KARS1 isoforms exert functional effects in cytosol and mitochondria. Bi-allelic pathogenic variants in KARS1 have been associated to sensorineural hearing and visual loss, neuropathy, seizures, and leukodystrophy. We report the clinical, biochemical, and neuroradiological features of nine individuals with KARS1-related disorder carrying 12 different variants with nine of them being novel. The consequences of these variants on the cytosol and/or mitochondrial LysRS were functionally validated in yeast mutants. Most cases presented with severe neurological features including congenital and progressive microcephaly, seizures, developmental delay/intellectual disability, and cerebral atrophy. Oculo-motor dysfunction and immuno-hematological problems were present in six and three cases, respectively. A yeast growth defect of variable severity was detected for most variants on both cytosolic and mitochondrial isoforms. The detrimental effects of two variants on yeast growth were partially rescued by lysine supplementation. Congenital progressive microcephaly, oculo-motor dysfunction, and immuno-hematological problems are emerging phenotypes in KARS1-related disorder. The data in yeast emphasize the role of both mitochondrial and cytosolic isoforms in the pathogenesis of KARS1-related disorder and supports the therapeutic potential of lysine supplementation at least in a subset of patients

Elastic scattering studies of 16C at 50 MeV/A on proton and deuteron targets with the CHIMERA multidetector at INFN-LNS

At the Laboratori Nazionali del Sud (LNS) in Catania (Italy), light radioactive ion beams have been produced through the In Flight Fragmentation method, using 18O and 13C at 55 MeV/A as primary beams impinging on a 9Be production target. Elastic scattering angular distributions of 16C+p and 16C+d at 50 MeV/A, 10Be+p at 56 MeV/A and 13B+d at 52 MeV/A systems were measured by using the CHIMERA (Charge Heavy Ion Mass and Energy Resolving Array) multidetector and kinematical coincidence technique. The experimental data are fitted by using the optical model. © Published under licence by IOP Publishing Ltd

Wild birds of the Italian Middle Ages: diet, environment and society

Wild birds are intrinsically associated with our perception of the Middle Ages. They often feature in heraldic designs, paintings, and books of hours; few human activities typify the medieval period better than falconry. Prominent in medieval iconography, wild birds feature less frequently in written sources (as they were rarely the subject of trade transactions or legal documents) but they can be abundant in archaeological sites. In this paper we highlight the nature of wild bird exploitation in Italian medieval societies, ranging from their role as food items to their status and symbolic importance. A survey of 13 Italian medieval sites corresponding to 19 ‘period sites’, dated from the fifth to the fifteenth centuries, reveals the occurrence of more than 100 species (certainly an under-estimate of the actual number). Anseriformes and Columbiformes played a prominent role in the mid- and late medieval Italian diet, though Passeriformes and wild Galliformes were also important. In the late Middle Ages, there is an increase in species diversity and in the role of hunting as an important marker of social status

Measurement of event shapes in deep inelastic scattering at HERA

Inclusive event-shape variables have been measured in the current region of the Breit frame for neutral current deep inelastic ep scattering using an integrated luminosity of 45.0 pb^-1 collected with the ZEUS detector at HERA. The variables studied included thrust, jet broadening and invariant jet mass. The kinematic range covered was 10 < Q^2 < 20,480 GeV^2 and 6.10^-4 < x < 0.6, where Q^2 is the virtuality of the exchanged boson and x is the Bjorken variable. The Q dependence of the shape variables has been used in conjunction with NLO perturbative calculations and the Dokshitzer-Webber non-perturbative corrections (`power corrections') to investigate the validity of this approach.Comment: 7+25 pages, 6 figure