Identification of exopolysaccharides-producing lactic acid bacteria from Burkina Faso fermented milk samples

Spacer region between 16S and 23 S rRNA genes of thirteen lactic acid bacteria strains from Burkina Faso fermented milk samples were amplified by the polymerase chain reaction (PCR). Lactobacillus delbrueckii, Lactobacillus acidophilus, Lactobacillus fermentum, Streptococcus thermophilus, Pediococcus spp, Leuconostoc mesenteroides subsp mesenteroides were identified. The Lactobacillus group was the predominant bacteria. Plasmids identified ranged between 2000 and 4000 bp. Exopolysaccharides (EPS) production varied from 181 mg/l and 814 mg/l, monomer analysis showed that glucose and galactose were predominant. Key Words: Lactic acid bacteria, fermented milk, PCR, exopolysaccharides. African Journal of Biotechnology Vol.3(3) 2004: 189-19

Phytochemical composition, Antioxidant and Anti-inflammatory potential of bioactive fractions from extracts of three medicinal plants traditionally used to treat liver diseases in Burkina Faso

Our aim in this study concerning the ethyl acetate and dichloromethane fractions was to provide a scientific basis for the treatment of hepatitis B in Burkina Faso of these three ethnomedicinal plants. As a result, we evaluated polyphenol content, antioxidant and anti-inflammatory evaluated by lipoxygenase (LOX) inhibitory and Xanthine Oxidase (XO) activities of aqueous acetone bioactive fractions from three species of Malvaceae (Sida cordifolia, Sida rhombifolia, S. urens). Folin-ciocalteu; AlCl3 methods and tannic acid respectively were used for polyphenol content research. The antioxidant activity of the samples was evaluate using three separate methods, inhibition of free radical 2,2-diphenyl-1-picrylhydramzyl (DPPH), ABTS radical cation decolorization assay, Iron (III) to iron (II) reduction activity (FRAP). For anti-inflammatoty activity, lypoxygenase and xanthine oxidase inhibitory activities were used. Finally, in this study, the ethyl acetate fraction has shown the best results comparatively to the dichloromethane fraction. Keywords: Polyphenol, Antioxidant, anti-inflammatory, Medicinal plants, hepatitis B

Detection of diarrheagenic Escherichia coli in human diarrheic stool and drinking water samples in Ouagadougou, Burkina Faso

Background: The presence of diarrheagenic Escherichia coli (DEC) in drinking water, is a grave public health problem. This study was aimed at characterization of diarrheagenic Escherichia coli isolated from drinking water and faecal samples from diarrheic patients in Ouagadougou, Burkina Faso.Materials and Methods: A total of 242 water samples consisting of 182 potable sachets and 60 from boreholes were collected in the period between October 2018 and April 2019 in the city of Ouagadougou. Faecal samples were also collected from 201 diarrheic patients visiting National Public Health Laboratory for a biological diagnosis by coproculture. The presence of virulence genes associated with DEC was determined by 16-plex polymerase chain reaction from bacteria culture.Results: From drinking water, we found 17% (42/242) Escherichia coli isolates in which 1% (2/242) DEC were detected. Among analyzed samples (182 sachet water versus 60 borehole water), the two DEC (01 ETEC and 01 EPEC) were detected in sachet water. DEC were detected in 20% (40/201) of patients. Enteroaggregative Escherichia coli (EAEC) were mostly detected in 10% followed by Enteropathogenic Escherichia coli (EPEC) in 4%, Enteroinvasive Escherichia coli (EIEC) in 2%, and Shiga toxin-producing Escherichia coli (STEC) 0.5%. However, Enterotoxigenic Escherichia coli (ETEC) was not detected alone, but in co-infections with EAEC.Conclusion: The present study documented the prevalence of Escherichia coli pathovars associated in patients with diarrhea, and shows that drinking water might be a source of DEC transmission in human

Phytochemical composition, Antioxidant and Anti-inflammatory potential of bioactive fractions from extracts of three medicinal plants traditionally used to treat liver diseases in Burkina Faso

Our aim in this study concerning the ethyl acetate and dichloromethane fractions was to provide a scientific basis for the treatment of hepatitis B in Burkina Faso of these three ethnomedicinal plants. As a result, we evaluated polyphenol content, antioxidant and anti-inflammatory evaluated by lipoxygenase (LOX) inhibitory and Xanthine Oxidase (XO) activities of aqueous acetone bioactive fractions from three species of Malvaceae (Sida cordifolia, Sida rhombifolia, S. urens). Folin-ciocalteu; AlCl3 methods and tannic acid respectively were used for polyphenol content research. The antioxidant activity of the samples was evaluate using three separate methods, inhibition of free radical 2,2-diphenyl-1-picrylhydramzyl (DPPH), ABTS radical cation decolorization assay, Iron (III) to iron (II) reduction activity (FRAP). For anti-inflammatoty activity, lypoxygenase and xanthine oxidase inhibitory activities were used. Finally, in this study, the ethyl acetate fraction has shown the best results comparatively to the dichloromethane fraction. Keywords: Polyphenol, Antioxidant, anti-inflammatory, Medicinal plants, hepatitis B

DETECTION OF DIARRHEAGENIC ESCHERICHIA COLI IN HUMAN DIARRHEIC STOOL AND DRINKING WATER SAMPLES IN OUAGADOUGOU, BURKINA FASO.

Background: The presence of diarrheagenic Escherichia coli (DEC) in drinking water, is a grave public health problem. This study was aimed at characterization of diarrheagenic Escherichia coli isolated from drinking water and faecal samples from diarrheic patients in Ouagadougou, Burkina Faso. Materials and Methods: A total of 242 water samples consisting of 182 potable sachets and 60 from boreholes were collected in the period between October 2018 and April 2019 in the city of Ouagadougou. Faecal samples were also collected from 201 diarrheic patients visiting National Public Health Laboratory for a biological diagnosis by coproculture. The presence of virulence genes associated with DEC was determined by 16-plex polymerase chain reaction from bacteria culture. Results: From drinking water, we found 17% (42/242) Escherichia coli isolates in which 1% (2/242) DEC were detected. Among analyzed samples (182 sachet water versus 60 borehole water), the two DEC (01 ETEC and 01 EPEC) were detected in sachet water. DEC were detected in 20% (40/201) of patients. Enteroaggregative Escherichia coli (EAEC) were mostly detected in 10% followed by Enteropathogenic Escherichia coli (EPEC) in 4%, Enteroinvasive Escherichia coli (EIEC) in 2%, and Shiga toxin-producing Escherichia coli (STEC) 0.5%. However, Enterotoxigenic Escherichia coli (ETEC) was not detected alone, but in co-infections with EAEC. Conclusion: The present study documented the prevalence of Escherichia coli pathovars associated in patients with diarrhea, and shows that drinking water might be a source of DEC transmission in human

Microbiological safety of flours used in follow up for infant formulas produced in Ouagadougou, Burkina Faso

The prevalence of diarrheal diseases in children aged from 6 to 24 months in Burkina Faso is 38%. These diarrheas may be due to the consumption of contaminated weaning food. Therefore, the microbiological quality of follow up infant flours used as supplement foods is a key-point to reduce children diseases. In this study, the microbiological safety of locally-produced infant flours was investigated. One hundred and ninety-nine (199) samples were collected mainly in retails outlets and in Recovery and Nutrition Education Centers. According to the Burkina Faso regulations, microbiological analyses were carried out for Total Aerobic Mesophilic Flora (TAMF), thermotolerant coliforms, Salmonella spp. and yeasts/molds. The bacterial and fungal isolates were identified using phenotypic and genotypic methods and the study of the production of mycotoxins was carried out from the fungal isolates. In collected samples, the TAMF count ranged from 0 to 1.8 × 106 CFU/g with a total average of 6.3 × 104 CFU/g. About 2% of the samples had a microbial load exceeding the standards (105 CFU/g). No Salmonella spp. was isolated in the final infant flours. However, the presence of Enterobacteriaceae (Klebsiella spp. Enterobacter spp. and Cronobacter spp.) was detected and molecular characterization revealed also the presence of fungal species of the genus Aspergillus spp., Penicillium spp. and Fusarium spp. Some of these species were found to produce aflatoxins, ochratoxin A and fumonisins, which are potential carcinogenic toxins. These results demonstrated the need for a preventive approach based on the application of Hazard Analysis Critical Control Point in the food industry to ensure food safety of infant flours in Burkina Faso

Antimicrobial activity of polyphenol-rich fractions from Sida alba L. (Malvaceae) against co-trimoxazol-resistant bacteria strains

<p>Abstract</p> <p>Background</p> <p>The increased resistance of microorganisms to the currently used antimicrobials has lead to the evaluation of other agents that might have antimicrobial activity. Medicinal plants are sources of phytochemicals which are able to initiate different biological activities including antimicrobials</p> <p>Materials and methods</p> <p><it>In vitro </it>antibacterial (MIC, MBC and time-kill studies) of polyphenol-rich fractions from <it>Sida alba </it>L. (Malvaceae) was assessed using ten bacteria strains (Gram-negative and Gram-positive).</p> <p>Results</p> <p>All test bacteria were susceptible to the polyphenol-rich fractions. Time-kill results showed that after 5 h exposition there was no viable microorganism in the initial inoculum and the effect of polyphenol-rich fractions was faster on <it>Enterococcus faecalis </it>(Gram-positive bacterium) comparatively to the other bacteria strains.</p> <p>Conclusion</p> <p>The data analysis indicates that the tested of polyphenol-rich fractions has significant effects when compared with the standard antibiotic. These results therefore justify the traditional use of <it>sida alba </it>L., alone or in combination with other herbs to treat bacterial infections.</p

Sonochemical degradation of triclosan in water and wastewater

The sonochemical degradation of 5 μg l−1 triclosan, a priority micro-pollutant, in various environmental samples (seawater, urban runoff and influent domestic wastewater) as well as in model solutions (pure and saline water) was investigated. Experiments were conducted with a horn-type sonicator operating at 80 kHz frequency and a nominal applied power of 135 W, while solid-phase microextraction coupled with gas chromatography–electron capture detector (SPME/GC–ECD) was employed to monitor triclosan degradation. The latter followed pseudo-first order kinetics with the rate constant being (min−1): 0.2284 for seawater > 0.1051 for 3.5% NaCl in deionised water > 0.0597 for centrifuged urban runoff ∼ 0.0523 for untreated urban runoff > 0.0272 for deionised water > 0.0063 for wastewater influent. SPME/GC–ECD and SPME coupled with gas chromatography–mass spectrometry (SPME/GC–MS) were also used to check for the formation of chlorinated and other toxic by-products; at the conditions in question, the presence of such compounds was not confirmedL.S.-P. and R.B. acknowledge the regional government Junta de Galicia for their doctoral and travel grantsS

Velocity Dispersions and Stellar Populations of the Most Compact and Msssive early-Type Galaxies at Redshift similar to 1

We present Gran-Telescopio-Canarias/OSIRIS optical spectra of four of the most compact and massive early-type galaxies (ETGs) in the Groth Strip Survey at redshift z similar to 1, with effective radii R-e = 0.5-2.4 kpc and photometric stellarmasses M-star = (1.2-4) x 10(11)M(circle dot). We find that these galaxies have velocity dispersions sigma = 156-236 km s(-1). The spectra are well fitted by single stellar population models with approximately 1 Gyr of age and solar metallicity. We find that (1) the dynamical masses of these galaxies are systematically smaller by a factor of similar to 6 than the published stellarmasses using BRIJK photometry, and (2) when estimating stellarmasses as 0.7xM(dyn), a combination of passive luminosity fading with mass/size growth due to minor mergers can plausibly evolve our objects to match the properties of the local population of ETGs

Bacterial and viral etiology of childhood diarrhea in Ouagadougou, Burkina Faso.

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