Phytochemical composition, Antioxidant and Anti-inflammatory potential of bioactive fractions from extracts of three medicinal plants traditionally used to treat liver diseases in Burkina Faso

Abstract

Our aim in this study concerning the ethyl acetate and dichloromethane fractions was to provide a scientific basis for the treatment of hepatitis B in Burkina Faso of these three ethnomedicinal plants. As a result, we evaluated polyphenol content, antioxidant and anti-inflammatory evaluated by lipoxygenase (LOX) inhibitory and Xanthine Oxidase (XO) activities of aqueous acetone bioactive fractions from three species of Malvaceae (Sida cordifolia, Sida rhombifolia, S. urens). Folin-ciocalteu; AlCl3 methods and tannic acid respectively were used for polyphenol content research. The antioxidant activity of the samples was evaluate using three separate methods, inhibition of free radical 2,2-diphenyl-1-picrylhydramzyl (DPPH), ABTS radical cation decolorization assay, Iron (III) to iron (II) reduction activity (FRAP). For anti-inflammatoty activity, lypoxygenase and xanthine oxidase inhibitory activities were used. Finally, in this study, the ethyl acetate fraction has shown the best results comparatively to the dichloromethane fraction. Keywords: Polyphenol, Antioxidant, anti-inflammatory, Medicinal plants, hepatitis B