81 research outputs found
Fully automated high-throughput chromatin immunoprecipitation for ChIP-seq: Identifying ChIP-quality p300 monoclonal antibodies
Chromatin immunoprecipitation coupled with DNA sequencing (ChIP-seq) is the major contemporary method for mapping in vivo protein-DNA interactions in the genome. It identifies sites of transcription factor, cofactor and RNA polymerase occupancy, as well as the distribution of histone marks. Consortia such as the ENCyclopedia Of DNA Elements (ENCODE) have produced large datasets using manual protocols. However, future measurements of hundreds of additional factors in many cell types and physiological states call for higher throughput and consistency afforded by automation. Such automation advances, when provided by multiuser facilities, could also improve the quality and efficiency of individual small-scale projects. The immunoprecipitation process has become rate-limiting, and is a source of substantial variability when performed manually. Here we report a fully automated robotic ChIP (R-ChIP) pipeline that allows up to 96 reactions. A second bottleneck is the dearth of renewable ChIP-validated immune reagents, which do not yet exist for most mammalian transcription factors. We used R-ChIP to screen new mouse monoclonal antibodies raised against p300, a histone acetylase, well-known as a marker of active enhancers, for which ChIP-competent monoclonal reagents have been lacking. We identified, validated for ChIP-seq, and made publicly available a monoclonal reagent called ENCITp300-1
Assessing unmet anaesthesia need in Sierra Leone: a secondary analysis of a cluster-randomized, cross-sectional, countrywide survey.
Objectives: To determine the unmet anaesthesia need in a low resource
region. Introduction: Surgery and an\ue6sthesia services in low- and
middle-income countries (LMICs) are under-equipped, under- staffed, and
unable to meet current surgical need. There is little objective measure
as to the true extent and nature of unmet need. Without such an
understanding it is impossible to formulate solutions. Therefore, we
re-examined Surgeons OverSeas (SOSAS) unmet surgical need data to
extrapolate unmet anaesthesia need. Methods: For the untreated surgical
conditions identified by SOSAS, we assigned anaesthetic technique
required to carry out the procedure. The chosen anaesthetic was based
on common practice in the region. Procedures were categorized into
minimal anaesthesia, spinal an\ue6sthesia, regional anaesthesia,
ketamine/monitored anaesthesia care (MAC), and general endotracheal
an\ue6sthesia (GETA). Discussions: Ninety-two per cent (687 of 745)
of untreated surgical conditions in Sierra Leone would require some
form of anaesthesia. Seventeen per cent (125 of 745) would require MAC,
22% (167 of 745) would require spinal anaesthesia, and 53% (395 of 745)
would require GETA. Conclusion: Analyses such as this can provide
guidance as to the rational and efficient production and distribution
of personnel, drugs and equipment
2,6-DideÂoxy-2,6-imino-l-glycero-d-ido-heptitol
The title molÂecule, C7H15NO5, the major product from selective enzymatic oxidation followed by hydrogenoÂlysis of the corresponding azidoÂheptitol, was found by X-ray crystallography to exisit in a chair conformation with three axial hydroxyl groups. One of the hydroxymethyl groups is disordered over two sets of sites in a 0.590â
(3):0.410â
(3) ratio. In the crystal, OâHâŻO, OâHâŻ(O,O), OâHâŻN and NâHâŻO hydrogen bonding occurs
Wide-field optical imaging on ELAIS N1, ELAIS N2, First Look Survey and Lockman Hole: observations and source catalogues
We present u-, g-, r-, i- and z-band optical images and associated catalogues taken primarily with the Isaac Newton Telescope Wide Field Camera on the European Large Area ISO Survey (ELAIS) N1 and N2, First Look Survey and Lockman Hole fields comprising a total of 1000 h of integration time over 80 deg^2 and approximately 4.3 million objects. In this paper we outline the observations and data processing and characterize the completeness, reliability, photometric and astrometric accuracy of this data set. All images have been photometrically calibrated using the Sloan Digital Sky Survey and a uniform and homogeneous data set is composed over all the observed fields. Magnitude limits are u, g, r, i, z of 23.9, 24.5, 24.0, 23.3, 22.0 (AB, 5Ï). These data have been used for optical identification of past and ongoing projects including the surveys ELAIS, Spitzer Wide-Area Infrared Extragalactic Survey, Spitzer Extragalactic Representative Volume Survey and Herschel Multi-tiered Extragalactic Survey
Calf health from birth to weaning. III. housing and management of calf pneumonia
Calfhood diseases have a major impact on the economic viability of cattle operations. A three part review series has been developed focusing on calf health from birth to weaning. In this paper, the last of the three part series, we review disease prevention and management with particular reference to pneumonia, focusing primarily on the pre-weaned calf. Pneumonia in recently weaned suckler calves is also considered, where the key risk factors are related to the time of weaning. Weaning of the suckler calf is often combined with additional stressors including a change in nutrition, environmental change, transport and painful husbandry procedures (castration, dehorning). The reduction of the cumulative effects of these multiple stressors around the time of weaning together with vaccination programmes (preconditioning) can reduce subsequent morbidity and mortality in the feedlot. In most studies, calves housed individually and calves housed outdoors with shelter, are associated with decreased risk of disease. Even though it poses greater management challenges, successful group housing of calves is possible. Special emphasis should be given to equal age groups and to keeping groups stable once they are formed. The management of pneumonia in calves is reliant on a sound understanding of aetiology, relevant risk factors, and of effective approaches to diagnosis and treatment. Early signs of pneumonia include increased respiratory rate and fever, followed by depression. The single most important factor determining the success of therapy in calves with pneumonia is early onset of treatment, and subsequent adequate duration of treatment. The efficacy and economical viability of vaccination against respiratory disease in calves remains unclear
A20 Modulates Lipid Metabolism and Energy Production to Promote Liver Regeneration
Background: Liver Regeneration is clinically of major importance in the setting of liver injury, resection or transplantation. We have demonstrated that the NF-B inhibitory protein A20 significantly improves recovery of liver function and mass following extended liver resection (LR) in mice. In this study, we explored the Systems Biology modulated by A20 following extended LR in mice. Methodology and Principal Findings: We performed transcriptional profiling using Affymetrix-Mouse 430.2 arrays on liver mRNA retrieved from recombinant adenovirus A20 (rAd.A20) and rAd.galactosidase treated livers, before and 24 hours after 78% LR. A20 overexpression impacted 1595 genes that were enriched for biological processes related to inflammatory and immune responses, cellular proliferation, energy production, oxidoreductase activity, and lipid and fatty acid metabolism. These pathways were modulated by A20 in a manner that favored decreased inflammation, heightened proliferation, and optimized metabolic control and energy production. Promoter analysis identified several transcriptional factors that implemented the effects of A20, including NF-B, CEBPA, OCT-1, OCT-4 and EGR1. Interactive scale-free network analysis captured the key genes that delivered the specific functions of A20. Most of these genes were affected at basal level and after resection. We validated a number of A20's target genes by real-time PCR, including p21, the mitochondrial solute carriers SLC25a10 and SLC25a13, and the fatty acid metabolism regulator, peroxisome proliferator activated receptor alpha. This resulted in greater energy production in A20-expressing livers following LR, as demonstrated by increased enzymatic activity of cytochrome c oxidase, or mitochondrial complex IV. Conclusion: This Systems Biology-based analysis unravels novel mechanisms supporting the pro-regenerative function of A20 in the liver, by optimizing energy production through improved lipid/fatty acid metabolism, and down-regulated inflammation. These findings support pursuit of A20-based therapies to improve patients' outcomes in the context of extreme liver injury and extensive LR for tumor treatment or donation
International Consensus Statement on Rhinology and Allergy: Rhinosinusitis
Background: The 5 years since the publication of the first International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICARâRS) has witnessed foundational progress in our understanding and treatment of rhinologic disease. These advances are reflected within the more than 40 new topics covered within the ICARâRSâ2021 as well as updates to the original 140 topics. This executive summary consolidates the evidenceâbased findings of the document. Methods: ICARâRS presents over 180 topics in the forms of evidenceâbased reviews with recommendations (EBRRs), evidenceâbased reviews, and literature reviews. The highest grade structured recommendations of the EBRR sections are summarized in this executive summary. Results: ICARâRSâ2021 covers 22 topics regarding the medical management of RS, which are grade A/B and are presented in the executive summary. Additionally, 4 topics regarding the surgical management of RS are grade A/B and are presented in the executive summary. Finally, a comprehensive evidenceâbased management algorithm is provided. Conclusion: This ICARâRSâ2021 executive summary provides a compilation of the evidenceâbased recommendations for medical and surgical treatment of the most common forms of RS
The synthesis and evaluation of iminosugars as glycosidase inhibitors
EThOS - Electronic Theses Online ServiceGBUnited Kingdo
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