Sensitivity Of Seedlings Of Black Cherry (Prunus serotina Ehrh.) To Ozone In Great Smoky Mountains National Park

The response of seedlings of black cherry (Prunus serotina Ehrh.) to ozone was evaluated in Great Smoky Mountains National Park using open-top chambers during the growing seasons of 1989 and 1992. Two separate sets of seedlings were each exposed to various concentrations of ozone (charcoal-filtered; 0 5 x (not used in 1989), 10 X , 1-5 X , and 2 0 x modified ambient) in two different seasons. Seasonal indices of exposure (SUMOO, SUM06 and AOT40) for the l O x treatments were 39 2, 19, and 1-62 p p m h respectively, in 1989, and 6 3 1 , 0 9, and 0-78 ppm h, respectively, in 1992. No significant chamber effects were noted, except for reduced height growth in open plots compared with 10 x chambers in 1992. In both years, the 2 0 x treatment reduced total, leaf, root, and shoot + root biomass, although some of these changes were only marginally significant in 1992. Stem biomass was significantly reduced in 1989, but not 1992. Leaf area, count and weight were all highly correlated, and showed significant reductions in both years. The leaf area ratio (leaf area/total weight) was reduced in 1989, but not in 1992. Height was not affected by ozone, but diameter was reduced only in 1989. Chamber-to-chamber variation for biomass and leaf variates was greater in 1992, and as a result, significance levels were lower. Weibull functions were fitted to chamber means, and showed significant near-linear declines for most components when log-transformed data were plotted against the SUM06 and AOT40 indices. Individual Weibull models for the 1989 and 1992 data sets, and combined models over both years, were developed. Combined models were adequate for describing ozone responses for all biomass components, as determined by the likelihood ratio test. The data show that the two years of exposure produced similar, but not identical effects, despite large differences in initial size of the seedlings and in seasonal ozone dynamics. Leaf and root biomass were most sensitive to ozone (as determined by the slope of decrease with increasing SUM06), whereas stem biomass was least sensitive. Black cherry seedlings are shown to be among the most sensitive to elevated ozone of the 21 tree species examined to date in Great Smoky Mountains National Park

Ozone Injury On Cutleaf Coneflower (Rudbeckia laciniata) And Crown-Beard (Verbesina occidentalis) In Great Smoky Mountains National Park

Incidence and severity of visible foliar ozone injury on cutleaf coneflower (Rudbeckia laciniata L.) and crown-beard (Verbesina occidentalis Walt.) were determined along selected trails at three locations in Great Smoky Mountains National Park during the summers of 2000 and 2001: Clingmans Dome, Cherokee Orchard Road and Purchase Knob. Cutleaf coneflower exhibited a greater amount of foliar injury than crown-beard each year of the 2-year study. Incidence and severity of injury was significantly greater for cutleaf coneflower growing near the edge of the Clingmans Dome trail than in the interior of the stand. Injury was greater at Clingmans Dome than Purchase Knob (70% vs. 40% ozone-injured plants, respectively), coincident with greater ozone exposures. In contrast to Clingmans Dome, there were no differences in injury between plants growing near- and off-trail at Purchase Knob. Differences in sensitivity to ozone were not observed for crown-beard growing near the edge compared with the interior of the stand adjacent to the Cherokee Orchard Road Loop. Ozone injury was greatest on the lower leaves for both species sampled with over 95% of the injured leaves occurring on the lower 50% of the plant. This is the first report of foliar ozone injury on these plant species in situ, in the Park, illustrating the great variability in symptom expression with time, and within and between populations

Interpreting Spatial Variation In Ozone Symptoms Shown By Cutleaf Cone Flower, Rudbeckia laciniata L.

Visible injury caused by ozone is recorded every year in native plant species growing in Great Smoky Mountains National Park (USA). One of the most sensitive species, cutleaf coneflower (Rudbeckia laciniata L.), shows great variation in symptoms between and within populations but the causes of this variation and its ecological significance are currently unknown. This paper presents data relating to genetic variation, ozone concentrations, stomatal conductance and light (PAR) within populations. The data show that populations differ in genetic diversity, one consisting of only three genets while another was very diverse. In the former population, symptoms varied greatly within a single genet, pointing to a large micro-environmental influence. Measurements of ozone, stomatal conductance and PAR within plant canopies suggest that variation in symptom expression is unlikely to be due to differences in ozone flux and more likely to be due to variation in light. The variation in visible symptoms raises the question of what bioindicators actually indicate, and it suggests that symptoms should be interpreted with great caution until the underlying causes of that variation are fully understood

Seedling Insensitivity To Ozone For Three Conifer Species Native To Great Smoky Mountains National Park

Field symptoms typical of ozone injury have been observed on several conifer species in Great Smoky Mountains National Park, and tropospheric ozone levels in the Park can be high, suggesting that ozone may be causing growth impairment of these plants. The objective of this research was to test the ozone sensitivity of selected conifer species under controlled exposure conditions. Seedlings of three species of conifers, Table Mountain pine (Pinus pungens), Virginia pine (Pinus virginiana), and eastern hemlock (Tsuga canadensis), were exposed to various levels of ozone in open-top chambers for one to three seasons in Great Smoky Mountains National Park in Tennessee, USA. A combination of episodic profiles (1988) and modified ambient exposure regimes (1989–92) were used. Episodic profiles simulated an average 7-day period from a monitoring station in the Park. Treatments used in 1988 were: charcoal-filtered (CF), 1.0× ambient, 2.0× ambient, and ambient air–no chamber (AA). In 1989 a 1.5× ambient treatment was added, and in 1990, additional chambers were made available, allowing a 0.5× ambient treatment to be added. Height, diameter, and foliar injury were measured most years. Exposures were 3 years for Table Mountain pine (1988–90), 3 years for hemlock (1989–91), and 1 and 2 years for three different sets of Virginia pine (1990, 1990–91, and 1992). There were no significant (p<0.05) effects of ozone on any biomass fraction for any of the species, except for older needles in Table Mountain and Virginia pine, which decreased with ozone exposure. There were also no changes in biomass allocation patterns among species due to ozone exposure, except for Virginia pine in 1990, which showed an increase in the root:shoot ratio. There was foliar injury (chlorotic mottling) in the higher two treatments (1.0× and 2.0× for Table Mountain and 2.0× for Virginia pine), but high plant-to-plant variability obscured formal statistical significance in many cases. We conclude, at least for growth in the short-term, that seedlings of these three conifer species are insensitive to ambient and elevated levels of ozone, and that current levels of ozone in the Park are probably having minimal impacts on these particular species

Photosynthetic Responses Of Microstegium vimineum (Trin.) A. Camus, A Shade Tolerant, C4 Grass, To Variable Light Environments

Microstegium vimineum (Trin.) A. Camus, a shade-tolerant C4 grass, has spread throughout the eastern United States since its introduction in 1919. This species invades disturbed understory habitats along streambanks and surrounding mesic forests, and has become a major pest in areas such as Great Smoky Mountains National Park. The focus of this study was to characterize the photosynthetic induction responses of M. vimineum, specifically its ability to utilize low light and sunflecks, two factors that may be critical to invasive abilities and survival in the understory. In addition, we were curious about the ability of a grass with the C4 photosynthetic pathway to respond to sunflecks. Plants were grown under 25% and 50% ambient sunlight, and photosynthetic responses to both steady-state and variable light were determined. Plants grown in both 25% and 50% ambient sun became 90% light saturated between 750–850 µmol m-2 s-1; however, plants grown in 50% ambient sun had significantly higher maximum steady-state photosynthetic rates (16.09 ± 1.37 µmol m-2 s-1 vs. 12.71 ± 1.18 µmol m-2 s-1). Both groups of plants induced to 50% of the steady-state rate in 3–5 min, while it took 10–13 min to reach 90% of maximum rates, under both flashing and steady-state light. For both groups of plants, stomatal conductance during induction reached maximum rates in 6–7 min, after which rates decreased slightly. Upon return to low light, rates of induction loss and stomatal closure were very rapid in both groups of plants, but were more rapid in those grown in high light. Rapid induction and the ability to induce under flashing light may enable this species to invade and dominate mesic understory habitats, while rapid induction loss due to stomatal closure may prevent excess water loss when low light constrains photosynthesis. The C4 pathway itself does not appear to present an insurmountable barrier to the ability of this grass species to respond to sunflecks in an understory environment

GDP: No one metric can rule them all

No abstract available

Tropospheric Ozone Assessment Report: present-day tropospheric ozone distribution and trends relevant to vegetation

This Tropospheric Ozone Assessment Report (TOAR) on the current state of knowledge of ozone metrics of relevance to vegetation (TOAR-Vegetation) reports on present-day global distribution of ozone at over 3300 vegetated sites and the long-term trends at nearly 1200 sites. TOAR-Vegetation focusses on three metrics over vegetation-relevant time-periods across major world climatic zones: M12, the mean ozone during 08:00–19:59; AOT40, the accumulation of hourly mean ozone values over 40 ppb during daylight hours, and W126 with stronger weighting to higher hourly mean values, accumulated during 08:00–19:59. Although the density of measurement stations is highly variable across regions, in general, the highest ozone values (mean, 2010–14) are in mid-latitudes of the northern hemisphere, including southern USA, the Mediterranean basin, northern India, north, north-west and east China, the Republic of Korea and Japan. The lowest metric values reported are in Australia, New Zealand, southern parts of South America and some northern parts of Europe, Canada and the USA. Regional-scale assessments showed, for example, significantly higher AOT40 and W126 values in East Asia (EAS) than Europe (EUR) in wheat growing areas (p < 0.05), but not in rice growing areas. In NAM, the dominant trend during 1995–2014 was a significant decrease in ozone, whilst in EUR it was no change and in EAS it was a significant increase. TOAR-Vegetation provides recommendations to facilitate a more complete global assessment of ozone impacts on vegetation in the future, including: an increase in monitoring of ozone and collation of field evidence of the damaging effects on vegetation; an investigation of the effects on peri-urban agriculture and in mountain/upland areas; inclusion of additional pollutant, meteorological and inlet height data in the TOAR dataset; where not already in existence, establishing new region-specific thresholds for vegetation damage and an innovative integration of observations and modelling including stomatal uptake of the pollutant

Contrasting drought tolerance strategies in two desert annuals of hybrid origin

Woody plants native to mesic habitats tend to be more vulnerable to drought-induced cavitation than those in xeric habitats. Cavitation resistance in herbaceous plants, however, is rarely studied and whether or not annual plants in arid habitats conform to the trends observed in woody plants is unknown. This question is addressed by comparing the hydraulic properties of annual plants endemic to relatively mesic and seasonally xeric habitats in the Great Basin Desert, in both native and experimental settings. Vulnerability to cavitation between species differed as predicted when vulnerability curves of similar-sized native individuals were compared. Contrary to expectations, Helianthus anomalus from the relatively mesic dune sites, on average, exhibited higher native embolism, lower soil-to-leaf hydraulic conductance (kL) and lower transpiration rates, than its xeric analogue, H. deserticola. In transplant gardens, H. anomalus’ vulnerability to cavitation was unaffected by transplant location or watering treatment. In H. deserticola, however, vulnerability to cavitation varied significantly in response to watering in transplant gardens and varied as a function of stem water potential (Ψstem). H. deserticola largely avoided cavitation through its higher water status and generally more resistant xylem, traits consistent with a short life cycle and typical drought-escape strategy. By contrast, H. anomalus’ higher native embolism is likely to be adaptive by lowering plant conductance and transpiration rate, thus preventing the loss of root-to-soil hydraulic contact in the coarse sand dune soils. For H. anomalus this dehydration avoidance strategy is consistent with its relatively long 3–4 month life cycle and low-competition habitat. We conclude that variance of hydraulic parameters in herbaceous plants is a function of soil moisture heterogeneity and is consistent with the notion that trait plasticity to fine-grained environmental variation can be adaptive

Deep Ly-alpha imaging of two z=2.04 GRB host galaxy fields

We report on the results of deep narrow-band Ly-alpha, U and I imaging of the fields of two GRBs at z=2.04 (GRB 000301C and GRB 000926). We find that the host galaxy of GRB 000926 is an extended, strong Ly-alpha emitter with a rest-frame equivalent width of 71+20-15 AA. The galaxy consists of two main components and several fainter knots. GRB 000926 occurred in the western component, whereas most of the Ly-alpha luminosity (about 65%) originates in the eastern component. Using archival HST images of the host galaxy we measure the spectral slopes (f_lambda prop. to lambda^beta) of the two components to beta = -2.4+-0.3 (east) and -1.4+-0.2 (west). This implies that both components contain at most small amounts of dust, consistent with the observed strong Ly-alpha emission. The western component has a slightly redder V-I colour than the eastern component, suggesting the presence of at least some dust. We do not detect the host galaxy of GRB 000301C in neither Ly-alpha emission nor in U and I-band images. We infer a limit of U(AB)>27.7 (2-sigma limit per arcsec^2). The upper limits on the Ly-alpha flux implies a Ly-alpha equivalent width upper limit of 150AA. We find 11 and 8 other galaxies with excess emission in the narrow filter in the fields of GRB 000301C and GRB 000926 respectively. Based on these detections we conclude that GRB 000926 occurred in one of the strongest centres of star formation within several Mpc, whereas GRB 000301C occurred in an intrinsically very faint galaxy far from being the strongest centre of star formation in its galactic environment. Under the hypothesis that GRBs trace star formation, the wide range of GRB host galaxy luminosities implies a very steep faint end slope of the high redshift galaxy luminosity function.Comment: 13 pages, 7 figures. Accepted for publication in A&

Tropospheric ozone assessment report: Global ozone metrics for climate change, human health, and crop/ecosystem research

Assessment of spatial and temporal variation in the impacts of ozone on human health, vegetation, and climate requires appropriate metrics. A key component of the Tropospheric Ozone Assessment Report (TOAR) is the consistent calculation of these metrics at thousands of monitoring sites globally. Investigating temporal trends in these metrics required that the same statistical methods be applied across these ozone monitoring sites. The nonparametric Mann-Kendall test (for significant trends) and the Theil-Sen estimator (for estimating the magnitude of trend) were selected to provide robust methods across all sites. This paper provides the scientific underpinnings necessary to better understand the implications of and rationale for selecting a specific TOAR metric for assessing spatial and temporal variation in ozone for a particular impact. The rationale and underlying research evidence that influence the derivation of specific metrics are given. The form of 25 metrics (4 for model-measurement comparison, 5 for characterization of ozone in the free troposphere, 11 for human health impacts, and 5 for vegetation impacts) are described. Finally, this study categorizes health and vegetation exposure metrics based on the extent to which they are determined only by the highest hourly ozone levels, or by a wider range of values. The magnitude of the metrics is influenced by both the distribution of hourly average ozone concentrations at a site location, and the extent to which a particular metric is determined by relatively low, moderate, and high hourly ozone levels. Hence, for the same ozone time series, changes in the distribution of ozone concentrations can result in different changes in the magnitude and direction of trends for different metrics. Thus, dissimilar conclusions about the effect of changes in the drivers of ozone variability (e.g., precursor emissions) on health and vegetation exposure can result from the selection of different metrics