Molecular clouds towards RCW 49 and Westerlund 2; Evidence for cluster formation triggered by cloud-cloud collision

We have made CO(J=2-1) observations towards the HII region RCW 49 and its ionizing source, the rich stellar cluster Westerlund 2 (hereafter Wd2), with the NANTEN2 sub-mm telescope. These observations have revealed that two molecular clouds in velocity ranges of -11 to +9 km/s and 11 to 21 km/s respectively, show remarkably good spatial correlations with the Spitzer IRAC mid-infrared image of RCW 49, as well a velocity structures indicative of localized expansion around the bright central regions and stellar cluster. This strongly argues that the two clouds are physically associated with RCW 49. We obtain a new kinematic distance estimate to RCW 49 and Wd2 of 5.4^{+ 1.1}_{- 1.4} kpc, based on the mean velocity and velocity spread of the associated gas. We argue that acceleration of the gas by stellar winds from Wd2 is insufficient to explain the entire observed velocity dispersion of the molecular gas, and suggest a scenario in which a collision between the two clouds ~4 Myrs ago may have triggered the formation of the stellar cluster.Comment: A version with higher resolution figures is available from http://www.a.phys.nagoya-u.ac.jp/~naoko/research/apjl2009/fur09_rev_highreso.pd

Prevalence of gambling disorder and its correlates among homeless men in Osaka city, Japan

Internationally, the prevalence of gambling disorder has been reported to be higher among homeless people than the general population; however, little is known about the factors associated with gambling disorder in this population. The present study aimed to investigate the prevalence of gambling disorder and its associated factors among homeless men using shelters in Osaka City. A cross-sectional survey was conducted from 30 to 2018 to 4 January 2019, using the 2017 Japanese National Survey of Gambling (JNSG) questionnaire, supplemented with questions about homeless experiences, drinking, and smoking. Using the South Oaks Gambling Screen, the presence of gambling disorder was determined by a score ≥ 5 out of 20. Multivariate logistic regression was conducted to explore factors associated with lifetime gambling disorder. Lifetime and past-year prevalence of gambling disorder among 103 participants was 43.7% (95% confidence interval [CI]: 34.5–53.3) and 3.9% (95% CI: 1.5–9.6), respectively, which are higher than the 6.7% and 1.5% found among men in the 2017 JNSG. Reasons reported for currently gambling less were primarily financial. Factors associated with lifetime GD included “more than 20 years since the first incidence of homelessness” (adjusted odds ratio [AOR]: 4.97, 95% CI: 1.50–16.45) and “more than five incidences of homelessness” (AOR: 4.51, 95% CI: 1.06–19.26). When homeless individuals with gambling disorder try to rebuild and stabilize their lives, the presence or resurgence of gambling disorder may hinder the process and pose a risk of recurring homelessness. Comprehensive support services for homeless individuals with gambling disorder are required. (250 words

看護学生の障がい者模擬体験学習による学び －感想文の内容分析を通して－

　看護系大学3年次学生のリハビリテーション看護授業の一環として，障がい者模擬体験に参加した学生の学びについて内容分析を用いて感想文を分析した．その結果，学生は，障害の種類の違いによる日常生活上の不自由さとその工夫の違い，障がい者の心理状態，障がい者を取り巻く環境とその援助の必要性，障がい者に対する否定的から肯定的な見方の変化，障がい者にとってのリハビリテーションの大切さ，などについて学んでいた．障がい者との関わりの少ない学生にとって，障がい者模擬体験は，リハビリテーション看護を学ぶうえで有効な方法であることが示唆された．Reports were analyzed using content analysis about the learning by students who participated in simulated experiences as handicapped persons as part of a rehabilitation nursing class of juniors in a nursing college. As a result, it was found that students had learned about such matters as differences in impairments in everyday life and their solutions depending on the type of handicap, the environment surrounding the handicapped and the necessity for support, change of view from negative to positive about the handicapped, and the importance of rehabilitation for the handicapped. It was suggested that simulated experiences as handicapped persons are an effective means for students who have little involvement with the handicapped to learn about rehabilitation nursing

Infrequent Mutation of Lysophosphatidic Acid Receptor-1 Gene in Hamster Pancreatic Duct Adenocarcinomas and Established Cell Lines

To evaluate the involvement of lysophosphatidic acid receptor-1 (LPA1) gene alteration in pancreatic carcinogenesis, we investigated mutations in the LPA1 gene in hamster pancreatic duct adenocarcinomas (PDAs) and established cell lines. Female Syrian golden hamsters received 30 mg/kg of N-nitrosobis(2-oxopropyl)amine (BOP) followed by repeated exposure to an augmentation pressure regimen consisting of a choline-deficient diet combined with DL-ethionine and then L-methionine and a further administration of 20 mg/kg BOP. A total of 10 PDAs obtained 10 weeks after beginning the experiment and three cell lines established from subcutaneously transplantable PDAs in syngeneic hamsters were examined for mutations using reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis. A mutation was detected in only one PDA (1/10, 10%) in the form of a GGA to GTA (Gly to Val) transversion at codon 355, and no mutations were detected in the three cell lines. These results suggest that the LPA1 gene mutation may play roles in a limited fraction of BOP-induced pancreatic duct carcinogenesis in hamsters

Transmission of survival signals through Delta-like 1 on activated CD4+ T cells

Notch expressed on CD4+ T cells transduces signals that mediate their effector functions and survival. Although Notch signaling is known to be cis-inhibited by Notch ligands expressed on the same cells, the role of Notch ligands on T cells remains unclear. In this report we demonstrate that the CD4+ T cell Notch ligand Dll1 transduces signals required for their survival. Co-transfer of CD4+ T cells from Dll1−/− and control mice into recipient mice followed by immunization revealed a rapid decline of CD4+ T cells from Dll1−/− mice compared with control cells. Dll1−/− mice exhibited lower clinical scores of experimental autoimmune encephalitis than control mice. The expression of Notch target genes in CD4+ T cells from Dll1−/− mice was not affected, suggesting that Dll1 deficiency in T cells does not affect cis Notch signaling. Overexpression of the intracellular domain of Dll1 in Dll1-deficient CD4+ T cells partially rescued impaired survival. Our data demonstrate that Dll1 is an independent regulator of Notch-signaling important for the survival of activated CD4+ T cells, and provide new insight into the physiological roles of Notch ligands as well as a regulatory mechanism important for maintaining adaptive immune responses

A peculiar jet and arc of molecular gas toward the rich and young stellar cluster Westerlund 2 and a TeV gamma ray source

We have discovered remarkable jet- and arc-like molecular features toward the rich and young stellar cluster Westerlund2. The jet has a length of ~100 pc and a width of ~10 pc, while the arc shows a crescent shape with a radius of ~30 pc. These molecular features each have masses of ~10000 solar mass and show spatial correlations with the surrounding lower density HI gas. The jet also shows an intriguing positional alignment with the core of the TeV gamma ray source HESS J1023-575 and with the MeV/GeV gamma-ray source recently reported by the Fermi collaboration. We argue that the jet and arc are caused by an energetic event in Westerlund 2, presumably due to an anisotropic supernova explosion of one of the most massive member stars. While the origin of the TeV and GeV gamma-ray sources is uncertain, one may speculate that they are related to the same event via relativistic particle acceleration by strong shock waves produced at the explosion or by remnant objects such as a pulsar wind nebula or microquasar

Discovery of Large-Scale Gravitational Infall in a Massive Protostellar Cluster

We report Mopra (ATNF), Anglo-Australian Telescope, and Atacama Submillimeter Telescope Experiment observations of a molecular clump in Carina, BYF73 = G286.21+0.17, which give evidence of large-scale gravitational infall in the dense gas. From the millimetre and far-infrared data, the clump has mass ~ 2 x 10^4 Msun, luminosity ~ 2-3 x 10^4 Lsun, and diameter ~ 0.9 pc. From radiative transfer modelling, we derive a mass infall rate ~ 3.4 x 10^-2 Msun yr-1. If confirmed, this rate for gravitational infall in a molecular core or clump may be the highest yet seen. The near-infrared K-band imaging shows an adjacent compact HII region and IR cluster surrounded by a shell-like photodissociation region showing H2 emission. At the molecular infall peak, the K imaging also reveals a deeply embedded group of stars with associated H2 emission. The combination of these features is very unusual and we suggest they indicate the ongoing formation of a massive star cluster. We discuss the implications of these data for competing theories of massive star formation.Comment: v1: 23 pages single-column, 6 figures (some multipart) at end v2: 14 pages 2-column, 6 figures interspersed v3: edited to referee's comments with new sections and new figures; accepted to MNRAS, 20 pages 2-column, 8 figures (some multipart) intersperse

Isolated total RNA and protein are preserved after thawing for more than twenty-four hours

OBJECTIVE: The preservation of biological samples at a low temperature is important for later biochemical and/or histological analyses. However, the molecular viability of thawed samples has not been studied sufficiently in depth. The present study was undertaken to evaluate the viability of intact tissues, tissue homogenates, and isolated total RNA after defrosting for more than twenty-four hours. METHODS: The molecular viability of the thawed samples (n = 82) was assessed using the A260/A280 ratio, the RNA concentration, the RNA integrity, the level of intact mRNA determined by reverse transcriptase polymerase chain reaction, the protein level determined by Western blotting, and an examination of the histological structure. RESULTS: The integrity of the total RNA was not preserved in the thawed intact tissue, but the RNA integrity and level of mRNA were perfectly preserved in isolated defrosted samples of total RNA. Additionally, the level of beta-actin protein was preserved in both thawed intact tissue and homogenates. CONCLUSION: Isolated total RNA does not undergo degradation due to thawing for at least 24 hours, and it is recommended to isolate the total RNA as soon as possible after tissue collection. Moreover, the protein level is preserved in defrosted tissues

髄液サイトカインプロファイルによる多巣性運動ニューロパチーと進行性筋萎縮症の鑑別

Objective: We aimed to compare the cytokine and chemokine profiles of patients with multifocal motor neuropathy (MMN) with those of patients with progressive muscular atrophy (PMA) and amyotrophic lateral sclerosis (ALS) to investigate immunologic differences in the CNS. Methods: CSF from 12 patients with MMN, 8 with PMA, 26 with sporadic ALS, and 10 with other noninflammatory neurologic disorders was analyzed for 27 cytokines and chemokines using the multiplex bead array assay. Cytokine titers of the 4 groups were compared, and correlations between the titers of relevant cytokines and clinical parameters were evaluated. Results: There were no obvious intrathecal changes except for interleukin (IL)-1 receptor antagonist in patients with MMN. In contrast, IL-4, IL-7, IL-17, eotaxin/CCL11, fibroblast growth factor- 2 (FGF-2), granulocyte colony-stimulating factor (G-CSF), and platelet-derived growth factor BB titers were significantly elevated in patients with PMA and ALS; of these, FGF-2 and G-CSF titers were elevated compared with those in patients with MMN. IL-4 and IL-10 titers were high in patients with ALS, particularly patients with possible ALS presenting with a slowly progressive course or mild symptoms. Conclusions: The CSF cytokine profile of patients with MMN is distinct from that of patients with PMA and ALS. The similarity of the cytokine profiles between patients with PMA and ALS suggests that PMA shares common immunologic features with ALS in the CNS, even without clinical evidence of upper motor neuron involvement