Biodegradable magnesium matrix composites for bone fixation devices

When a bone is fractured, it loses its structural integrity which makes it unable to bear any mechanical load. Therefore, a broken bone must be supported until it regains its strength to handle the body's movement and weight. A surgical procedure is needed to set a fractured bone. This procedure often involves repositioning the bone fragments into their natural position and then, attaching them together using internal fixation devices such as plates and screws. These fixation devices restore load-beanng capacity to bone, allowing the fractured bone to be healed by the primary bone healing mechanism. To date, implants used for internal fixadon are usually made from titanium and stainless steel, which are strong but, notorious for triggering adverse reactions such allergic responses caused by implant erosion in patients. Therefore, permanent fixtures should be removed from the body after the fractured bone heals sufficiently, which imposes another invasive surgery on the patient. The advent of biodegradable magnesium-based composites about two decades ago was an attempt to address the clinical complications regarding the permanent fixtures. However, magnesium-based composites are still in their infancy, and a have a lot to achieve before being considered as fully functional materials for bone fixation purposes. Currently, there are two major issues with magnesium composites. Firstly, most of the magnesium-based composites made to date lack sufficient mechanical integrity, making them unsuitable for load-bearing applications. The second, and the most important, issue would be the rapid degradation of magnesium when exposed to physiological solutions, causing pre-mature mechanical failure before the patient fully recovers. The main aim of this thesis is to provide the necessary background and technical information to address these issues, and to be a reliable platform for future researches on the subject to build upon.Biomaterials & Tissue Biomechanic

Influence of HEPES buffer on the local pH and formation of surface layer during in vitro degradation tests of magnesium in DMEM

The human body is a buffered environment where pH is effectively maintained. HEPES is a biological buffer often used to mimic the buffering activity of the body in in vitro studies on the degradation behavior of magnesium. However, the influence of HEPES on the degradation behavior of magnesium in the DMEM pseudo-physiological solution has not yet been determined. The research aimed at elucidating the degradation mechanisms of magnesium in DMEM with and without HEPES. The morphologies and compositions of surface layers formed during in vitro degradation tests for 15–3600 s were characterized. The effect of HEPES on the electrochemical behavior and corrosion tendency was determined by performing electrochemical tests. HEPES indeed retained the local pH, leading to intense intergranular/interparticle corrosion of magnesium made from powder and an increased degradation rate. This was attributed to an interconnected network of cracks formed at the original powder particle boundaries and grain boundaries in the surface layer, which provided pathways for the corrosive medium to interact continuously with the internal surfaces and promoted further dissolution. Surface analysis revealed significantly reduced amounts of precipitated calcium phosphates due to the buffering activity of HEPES so that magnesium became less well protected in the buffered environment.Materials Science and EngineeringMechanical, Maritime and Materials Engineerin

Advanced bredigite-containing magnesium-matrix composites for biodegradable bone implant applications

The present research was aimed at developing magnesium-matrix composites that could allow effective control over their physiochemical and mechanical responses when in contact with physiological solutions. A biodegradable, bioactive ceramic - bredigite was chosen as the reinforcing phase in the composites, based on the hypothesis that the silicon- and magnesium-containing ceramic could protect magnesium from fast corrosion and at the same time stimulate cell proliferation. Methods to prepare composites with integrated microstructures - a prerequisite to achieve controlled biodegradation were developed. A systematic experimental approach was taken in order to elucidate the in vitro biodegradation mechanisms and kinetics of the composites. It was found that the composites with 20–40% homogenously dispersed bredigite particles, prepared from powders, could indeed significantly decrease the degradation rate of magnesium by up to 24 times. Slow degradation of the composites resulted in the retention of the mechanical integrity of the composites within the strength range of cortical bone after 12 days of immersion in a cell culture medium. Cell attachment, cytotoxicity and bioactivity tests confirmed the stimulatory effects of bredigite embedded in the composites on the attachment, viability and differentiation of bone marrow stromal cells. Thus, the multiple benefits of adding bredigite to magnesium in enhancing degradation behavior, mechanical properties, biocompatibility and bioactivity were obtained. The results from this research showed the excellent potential of the bredigite-containing composites for bone implant applications, thus warranting further in vitro and in vivo research.Accepted Author Manuscript(OLD) MSE-1Biomaterials & Tissue Biomechanics(OLD) MSE-