3,916 research outputs found
Apm4, the mu subunit of yeast AP-2 interacts with Pkc1, and mutation of the Pkc1 consensus phosphorylation site Thr176 inhibits AP-2 recruitment to endocytic sites.
The AP-2 endocytic adaptor has been extensively characterized in mammalian cells and is considered to play a role both in cargo binding and in formation of endocytic sites. However, despite our detailed knowledge of mechanistic aspects of endocytic complex assembly and disassembly in the model organism Saccharomyces cerevisiae, no function of AP-2 had been described in wild-type yeast under normal growth conditions. A recent study however revealed that disruption of the complex caused by deletion of the gene encoding its mu subunit (APM4) caused defects in cell polarity such that responses to pheromone, nutritional status and cell wall damage were affected. Furthermore, a homozygous deletion of the mu subunit gene in Candida albicans affected its ability to grow hyphae. Direct binding to the yeast cell wall stress sensor Mid2 was detected, and in an apm4 deletion strain Mid2 showed reduced re-localization to the mother bud neck region following cell wall damage with calcofluor or to the mating projection tip. Here we demonstrate an interaction between Apm4 and the yeast cell wall integrity pathway component Pkc1 and show that mutation of the predicted Pkc1 site in the Apm4 hinge region affects recruitment of the AP-2 complex to endocytic sites
Progression of myopathology in Kearns-Sayre syndrome
We report on the progression of myopathology by comparing two biopsies from a patient with a Kearns-Sayre-Syndrome. The first biopsy was taken in 1979 and showed 10% ragged-red fibers. Myopathic changes were slight including internal nuclei and fiber splitting in 10% of the fibers. Electron microscopy revealed typical mitochondrial abnormalities with regard to number and shape. In 1989 a second biopsy was performed for an extended analysis of mitochondrial DNA. This time less than 5% of all fibers were ragged-red. Severe myopathic changes could be detected which so far has rarely been reported in mitochondrial cytopathy
Perceptions of knowledge sharing among small family firm leaders: a structural equation model
Small family firms have many unique relational qualities with implications for how knowledge is passed between individuals. Extant literature posits leadership approach as important in explaining differences in knowledge-sharing climate from one firm to another. This study investigates how leadership approaches interact with family influence to inform perceptions of knowledge sharing. We utilize survey data (n = 110) from owner-managers of knowledge-intensive small family firms in Scotland. Our findings present a choice in leadership intention, contrasting organization-focused participation against family-influenced guidance. Insight is offered on the implications of this leadership choice at both organizational and familial level
1+1 Dimensional Hydrodynamics for High-energy Heavy-ion Collisions
A 1+1 dimensional hydrodynamical model in the light-cone coordinates is used
to describe central heavy-ion collisions at ultrarelativistic bombarding
energies. Deviations from Bjorken's scaling are taken into account by choosing
finite-size profiles for the initial energy density. The sensitivity of fluid
dynamical evolution to the equation of state and the parameters of initial
state is investigated. Experimental constraints on the total energy of produced
particles are used to reduce the number of model parameters. Spectra of
secondary particles are calculated assuming that the transition from the
hydrodynamical stage to the collisionless expansion of matter occurs at a
certain freeze-out temperature. An important role of resonances in the
formation of observed hadronic spectra is demonstrated. The calculated rapidity
distributions of pions, kaons and antiprotons in central Au+Au collisions at
the c.m. energy 200 GeV per NN pair are compared with experimental data of the
BRAHMS Collaboration. Parameters of the initial state are reconstructed for
different choices of the equation of state. The best fit of these data is
obtained for a soft equation of state and Gaussian-like initial profiles of the
energy density, intermediate between the Landau and Bjorken limits.Comment: 43 pages, 27 figure
Directed and Elliptic Flow at RHIC
We present the directed flow measurement () from Au+Au collisions at
\sqrtsNN = 62 GeV. Over the pseudorapidity range we have studied, which
covers from -1.2 to 1.2 and , the magnitude of
for charged particles is found to increase monotonously with pseudorapidity for
all centralities. No `` wiggle'', as predicted by various theoretical
models, is observed at midrapidity. Elliptic flow () from moderate high
particles () at \sqrtsNN = 200 GeV is presented as a
function of impact parameter. It is found that models that are based on {\it
jet quenching} alone appear to underpredict at moderate high , while
the model that incorporates both, recombination and fragmentation, describes
the data better.Comment: 6 pages, 4 figures. Proceeding for Hot Quark 04 conference Changes in
the revision are mostly English fixes. v1 versus eta plot is flipped over to
follow the conventio
Forbush decreases and turbulence levels at CME fronts
We seek to estimate the average level of MHD turbulence near coronal mass
ejection (CME) fronts as they propagate from the Sun to the Earth. We examine
the cosmic ray data from the GRAPES-3 tracking muon telescope at Ooty, together
with the data from other sources for three well observed Forbush decrease
events. Each of these events are associated with frontside halo Coronal Mass
Ejections (CMEs) and near-Earth magnetic clouds. In each case, we estimate the
magnitude of the Forbush decrease using a simple model for the diffusion of
high energy protons through the largely closed field lines enclosing the CME as
it expands and propagates from the Sun to the Earth. We use estimates of the
cross-field diffusion coefficient derived from published results of
extensive Monte Carlo simulations of cosmic rays propagating through turbulent
magnetic fields. Our method helps constrain the ratio of energy density in the
turbulent magnetic fields to that in the mean magnetic fields near the CME
fronts. This ratio is found to be 2% for the 11 April 2001 Forbush
decrease event, 6% for the 20 November 2003 Forbush decrease event and
249% for the much more energetic event of 29 October 2003.Comment: Accepted for publication in Astronomy and Astrophysics. (Abstract
abridged) Typos correcte
Discrete cilia modelling with singularity distributions
We discuss in detail techniques for modelling flows due to finite and infinite arrays of beating cilia. An efficient technique, based on concepts from previous ‘singularity models’ is described, that is accurate in both near and far-fields. Cilia are modelled as curved slender ellipsoidal bodies by distributing Stokeslet and potential source dipole singularities along their centrelines, leading to an integral equation that can be solved using a simple and efficient discretisation. The computed velocity on the cilium surface is found to compare favourably with the boundary condition. We then present results for two topics of current interest in biology. 1) We present the first theoretical results showing the mechanism by which rotating embryonic nodal cilia produce a leftward flow by a ‘posterior tilt,’ and track particle motion in an array of three simulated nodal cilia. We find that, contrary to recent suggestions, there is no continuous layer of negative fluid transport close to the ciliated boundary. The mean leftward particle transport is found to be just over 1 μm/s, within experimentally measured ranges. We also discuss the accuracy of models that represent the action of cilia by steady rotlet arrays, in particular, confirming the importance of image systems in the boundary in establishing the far-field fluid transport. Future modelling may lead to understanding of the mechanisms by which morphogen gradients or mechanosensing cilia convert a directional flow to asymmetric gene expression. 2) We develop a more complex and detailed model of flow patterns in the periciliary layer of the airway surface liquid. Our results confirm that shear flow of the mucous layer drives a significant volume of periciliary liquid in the direction of mucus transport even during the recovery stroke of the cilia. Finally, we discuss the advantages and disadvantages of the singularity technique and outline future theoretical and experimental developments required to apply this technique to various other biological problems, particularly in the reproductive system
Investigating knowledge management factors affecting Chinese ICT firms performance: An integrated KM framework
This is an Author's Accepted Manuscript of an article published in the Journal of Information Systems Management, 28(1), 19 - 29, 2011, copyright Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/10580530.2011.536107.This article sets out to investigate the critical factors of Knowledge Management (KM) which are considered to have an impact on the performance of Chinese information and communication technology (ICT) firms. This study confirms that the cultural environment of an enterprise is central to its success in the context of China. It shows that a collaborated, trusted, and learning environment within ICT firms will have a positive impact on their KM performance
Moebius strip enterprises and expertise in the creative industries: new challenges for lifelong learning?
The paper argues that the emergence of a new mode of production – co-configuration is generating new modes of expertise that EU policies for lifelong learning are not designed to support professionals to develop. It maintains that this change can be seen most clearly when we analyse Small and Medium Size (SMEs) enterprises in the creative industries. Drawing on concepts from Political Economy - ‘Moebius strip enterprise/expertise’ and Cultural Historical Activity Theory - project-object’ and the ‘space of reasons’, the paper highlights conceptually and through a case study of an SME in the creative industries what is distinctive about the new modes of expertise, before moving on to reconceptualise expertise and learning and to consider the implications of this reconceptualisation for EU policies for lifelong learning. The paper concludes that the new challenge for LLL is to support the development of new forms expertise that are difficult to credentialise, yet, are central to the wider European goal of realising a knowledge economy
Recombination Models
We review the current status of recombination and coalescence models that
have been successfully applied to describe hadronization in heavy ion
collisions at RHIC energies. Basic concepts as well as actual implementations
of the idea are discussed. We try to evaluate where we stand in our
understanding at the moment and what remains to be done in the future.Comment: Plenary Talk at Quark Matter 2004, submitted to J. Phys. G, 8 pages,
3 figure
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