Differing patterns of selection and geospatial genetic diversity within two leading Plasmodium vivax candidate vaccine antigens

Although Plasmodium vivax is a leading cause of malaria around the world, only a handful of vivax antigens are being studied for vaccine development. Here, we investigated genetic signatures of selection and geospatial genetic diversity of two leading vivax vaccine antigens--Plasmodium vivax merozoite surface protein 1 (pvmsp-1) and Plasmodium vivax circumsporozoite protein (pvcsp). Using scalable next-generation sequencing, we deep-sequenced amplicons of the 42 kDa region of pvmsp-1 (n = 44) and the complete gene of pvcsp (n = 47) from Cambodian isolates. These sequences were then compared with global parasite populations obtained from GenBank. Using a combination of statistical and phylogenetic methods to assess for selection and population structure, we found strong evidence of balancing selection in the 42 kDa region of pvmsp-1, which varied significantly over the length of the gene, consistent with immune-mediated selection. In pvcsp, the highly variable central repeat region also showed patterns consistent with immune selection, which were lacking outside the repeat. The patterns of selection seen in both genes differed from their P. falciparum orthologs. In addition, we found that, similar to merozoite antigens from P. falciparum malaria, genetic diversity of pvmsp-1 sequences showed no geographic clustering, while the non-merozoite antigen, pvcsp, showed strong geographic clustering. These findings suggest that while immune selection may act on both vivax vaccine candidate antigens, the geographic distribution of genetic variability differs greatly between these two genes. The selective forces driving this diversification could lead to antigen escape and vaccine failure. Better understanding the geographic distribution of genetic variability in vaccine candidate antigens will be key to designing and implementing efficacious vaccines

Sugarcane (Saccharum X officinarum): A Reference Study for the Regulation of Genetically Modified Cultivars in Brazil

Global interest in sugarcane has increased significantly in recent years due to its economic impact on sustainable energy production. Sugarcane breeding and better agronomic practices have contributed to a huge increase in sugarcane yield in the last 30 years. Additional increases in sugarcane yield are expected to result from the use of biotechnology tools in the near future. Genetically modified (GM) sugarcane that incorporates genes to increase resistance to biotic and abiotic stresses could play a major role in achieving this goal. However, to bring GM sugarcane to the market, it is necessary to follow a regulatory process that will evaluate the environmental and health impacts of this crop. The regulatory review process is usually accomplished through a comparison of the biology and composition of the GM cultivar and a non-GM counterpart. This review intends to provide information on non-GM sugarcane biology, genetics, breeding, agronomic management, processing, products and byproducts, as well as the current technologies used to develop GM sugarcane, with the aim of assisting regulators in the decision-making process regarding the commercial release of GM sugarcane cultivars

Man and the Last Great Wilderness: Human Impact on the Deep Sea

The deep sea, the largest ecosystem on Earth and one of the least studied, harbours high biodiversity and provides a wealth of resources. Although humans have used the oceans for millennia, technological developments now allow exploitation of fisheries resources, hydrocarbons and minerals below 2000 m depth. The remoteness of the deep seafloor has promoted the disposal of residues and litter. Ocean acidification and climate change now bring a new dimension of global effects. Thus the challenges facing the deep sea are large and accelerating, providing a new imperative for the science community, industry and national and international organizations to work together to develop successful exploitation management and conservation of the deep-sea ecosystem. This paper provides scientific expert judgement and a semi-quantitative analysis of past, present and future impacts of human-related activities on global deep-sea habitats within three categories: disposal, exploitation and climate change. The analysis is the result of a Census of Marine Life – SYNDEEP workshop (September 2008). A detailed review of known impacts and their effects is provided. The analysis shows how, in recent decades, the most significant anthropogenic activities that affect the deep sea have evolved from mainly disposal (past) to exploitation (present). We predict that from now and into the future, increases in atmospheric CO2 and facets and consequences of climate change will have the most impact on deep-sea habitats and their fauna. Synergies between different anthropogenic pressures and associated effects are discussed, indicating that most synergies are related to increased atmospheric CO2 and climate change effects. We identify deep-sea ecosystems we believe are at higher risk from human impacts in the near future: benthic communities on sedimentary upper slopes, cold-water corals, canyon benthic communities and seamount pelagic and benthic communities. We finalise this review with a short discussion on protection and management methods

Viabilidade agronômica do consórcio de alface e rúcula, em duas épocas de cultivo Agronomic viability of lettuce-roquette intercropping in two growing periods

Avaliou-se a produtividade de grupos de alface e da rúcula, em consórcio, em relação aos seus cultivos solteiros, na UNESP Jaboticabal, em condições de campo, em duas épocas de cultivo, maio a agosto e setembro a novembro de 2001, sob delineamento de blocos casualizados, com quatro repetições. Os tratamentos constaram de combinações dos fatores grupos de alface (crespa, cv. Vera; lisa, cv. Elisa e americana, cv. Tainá), sistemas de cultivo (consórcio e cultivo solteiro) e épocas de semeadura da rúcula para o estabelecimento do consórcio [0; 7 e 14 dias após o transplante (DAT) da alface]. As maiores matérias fresca e seca de alface foram obtidas na primavera, com destaque para a alface americana. As alfaces não foram afetadas pelo sistema de cultivo. A maior matéria fresca de rúcula, no outono-inverno, foi obtida em consórcio, a 0 DAT, com as alfaces crespa e lisa e, aos 7 DAT com americana, enquanto na primavera, quando foi consorciada aos 7 DAT com alface crespa e a 0 DAT com alfaces do grupo lisa e americana. A rúcula teve sua matéria seca reduzida nos consórcios estabelecidos tardiamente, aos 14 DAT. Os cultivos consorciados apresentaram-se superiores aos cultivos solteiros entre 5 e 93%, segundo o índice de uso eficiente da terra. Os maiores índices de uso eficiente da terra foram obtidos com os consórcios de rúcula e alface crespa a 0 DAT (1,93), no outono-inverno e pelas mesmas hortaliças aos 7 DAT (1,84), na primavera.<br>The productivity of different lettuce groups and roquette was evaluated when sown in two different periods and cultivated either singly or intercropped. Theses experiments were carried out in Jaboticabal, São Paulo State, Brazil. The first growing period was from May to August (Autumn-Winter) and the second from September to November (Spring) of 2001. The experimental design was a randomized complete block with four replications. The treatments consisted of the combination of the following factors levels: lettuce groups (crisp, cv. Vera; looseleaf, cv. Elisa; and crisphead lettuce, cv. Tainá), cropping systems (intercropping and sole crop) and roquette sowing times [0; 7, and 14 days after lettuce transplantating (DAT)]. The highest fresh and dry matter production were observed for crisphead lettuce in the Spring. The cropping systems did not affect the lettuce groups productivity. The highest roquette fresh matter production was observed in the Autumn-Winter period at 0 DAT, with the crisp and looseleaf lettuce, and at 7 DAT with crisphead lettuce. For the Spring period the highest roquette fresh matter production was observed at 7 DAT with crisp lettuce and at 0 DAT with looseleaf and the crisphead lettuce. Roquette dry matter was reduced in the intercropping at 14 DAT. The intercropping system yielded 5 to 93% more than the single system according to the land equivalent ratio. The highest land equivalent ratio was observed in the roquette-crisp lettuce intercropping at 0 DAT the crisp variety (1.93) in the Autumn-Winter, while the highest ratio was observed at 7 DAT (1.84) in the Spring

Does malaria suffer from lack of memory?

It is widely perceived that immunity to malaria is, to an extent, defective and that one component of this defective immune response is the inability to induce or maintain long-term memory responses. If true, this is likely to pose problems for development of an effective vaccine against malaria. In this article, we critically review and challenge this interpretation of the epidemiological and experimental evidence. While evasion and modulation of host immune responses clearly occurs and naturally acquired immunity is far from optimal, mechanisms to control blood-stage parasites are acquired and maintained by individuals living in endemic areas, allowing parasite density to be kept below the threshold for induction of acute disease. Furthermore, protective immunity to severe pathology is achieved relatively rapidly and is maintained in the absence of boosting by re-infection. Nevertheless, there are significant challenges to overcome. The need for multiple infections to acquire immunity means that young children remain at risk of infection for far too long. Persistent or frequent exposure to antigen seems to be required to maintain anti-parasite immunity (premunition). Lastly, pre-erythrocytic and sexual stages of the life cycle are poorly immunogenic, and there is little evidence of effective pre-erythrocytic or transmission-blocking immunity at the population level. While these problems might theoretically be due to defective immunological memory, we suggest alternative explanations. Moreover, we question the extent to which these problems are malaria-specific rather than generic (i.e. result from inherent limitations of the vertebrate immune system)