Sexual dimorphism in cervical vertebral canal measurements of human foetuses

Little is known about postembryonic development of the human vertebral canal. Cervical parts of vertebral canal in 30 normal human foetuses was exposed in coronal plane and were divided in groups 1 and 2 which correspond with 2nd and 3rd trimester of pregnancy respectively. Groups 1 and 2 included 18 and 12 foetuses respectively with equal number of males and females in each. Length of cervical part of spinal canal and transverse diameter with height at different vertebral levels were recorded. Sexual dimorphism was noticed in foetuses of third trimester only. Length of cervical canal and height of vertebral bodies were significantly more in males while transverse diameter was statistically high in females. Cervical part of vertebral canal in human fetuses displays sexual dimorphism. Males have narrower and longer spinal canal compared to females.Keywords: foetuses, cervical, vertebral canal, dimorphis

17P. Building ICT Success Using PBL Based Practices

Increasingly, nowadays students must learn more advanced technical skills in order to secure jobs in a competitive 21st century workforce. This is true even in the Gulf Countries, including the UAE, where less IT work opportunities are available and where competition to find a good IT job is becoming harder. Yet the curriculum that was available to teach them these cutting-edge skills was often textbook-based, uninspiring, and limited in its appeal. Therefore, it was essential to address these issues at the curriculum of developmental program of United Arab Emirates University (UAEU) before students move on to their specific discipline. Considering the earlier mentioned flaws in IT, UGRU curriculum, leadership team in spring 2005 decided to introduce critical and creative thinking and cognition based ICT literacy program along with PBL (Problem Based Learning). The basic objectives of the PBL projects are to promote (i) Research, (ii) Critical Thinking, and (iii) Application of ICT knowledge. The themes of the projects are based on geo-social, scientific or general awareness. In this paper the IT UGRU developmental program will be compared to those counterparts that exist in leading Universities such as UAE, Kingdom of Saudi Arabia, Qatar, Australia, New Zealand, Norway, UK, Canada, and USA. This paper describes the PBL based teaching strategies and their overall effectiveness. It also discusses the impact of these projects on student learning and achieving educational goals. Results are very encouraging. The outcome of this research indicates that using PBL in ICT classrooms can help students become self learners, able to search out, understand, analyze, and synthesize information in a better way. It also helps them to understand the real value of ICT in their lives

Learning to Think while Thinking to Learn: Promoting the Infusion of Critical and Creative Thinking in Today\u27s ICT Classrooms

Traditional IT courses usually require enormous student motivation and perseverance with varying results. IT lecturers at UAE University’s University General Requirements Program tackled these problems by promoting a natural way of infusing creative and critical thinking in the classroom by structuring lessons where students manage their own thinking. Three main critical and creative thinking methodologies (Open Compare and Contrast, Focused Compare and Contrast, and Determining Parts-Whole Relationships) were used with five ICT sections (about 100 students). This paper describes the new lesson plans, their overall effectiveness, and future plans. It also discusses the impact of these lessons on student learning and also in terms of educational goals, contents, and assessment (periodic and terminal). The outcome of this research indicates that changes in ICT classroom teaching methods can help students become critical thinkers, able to search out, understand, analyze, and synthesize information

Infusing Critical and Creative Thinking and Metacognition in ICT Education: A Classroom Study

Enormous student motivation and perseverance are required for Traditional IT courses. To overcome these problems, IT lecturers at UAE University’s University General Requirements Program have promoted a natural way of infusing creative and critical thinking in the classroom by structuring lessons in which students manage their own thinking, not a physical performance in the class. Three main critical and creative thinking methodologies (Open Compare and Contrast, Focused Compare and Contrast, and Determining Parts-Whole Relationships) were used with five ICT sections (about 100 students). This paper describes the new lesson plans, their overall effectiveness, and future plans. It also discusses the impact of these lessons on student learning and comprehension and also in terms of educational goals, contents, and assessment. The outcome of this research indicates that ICT classroom teaching methods changes will help students to become critical thinkers, able to search out, understand, analyze, and synthesize information

BLM and RMI1 alleviate RPA inhibition of topoIIIα decatenase activity

RPA is a single-stranded DNA binding protein that physically associates with the BLM complex. RPA stimulates BLM helicase activity as well as the double Holliday junction dissolution activity of the BLM-topoisomerase IIIα complex. We investigated the effect of RPA on the ssDNA decatenase activity of topoisomerase IIIα. We found that RPA and other ssDNA binding proteins inhibit decatenation by topoisomerase IIIα. Complex formation between BLM, TopoIIIα, and RMI1 ablates inhibition of decatenation by ssDNA binding proteins. Together, these data indicate that inhibition by RPA does not involve species-specific interactions between RPA and BLM-TopoIIIα-RMI1, which contrasts with RPA modulation of double Holliday junction dissolution. We propose that topoisomerase IIIα and RPA compete to bind to single-stranded regions of catenanes. Interactions with BLM and RMI1 enhance toposiomerase IIIα activity, promoting decatenation in the presence of RPA

A synthetic biology approach to probing nucleosome symmetry

The repeating subunit of chromatin, the nucleosome, includes two copies of each of the four core histones, and several recent studies have reported that asymmetrically-modified nucleosomes occur at regulatory elements in vivo. To probe the mechanisms by which histone modifications are read out, we designed an obligate pair of H3 heterodimers, termed H3X and H3Y, which we extensively validated genetically and biochemically. Comparing the effects of asymmetric histone tail point mutants with those of symmetric double mutants revealed that a single methylated H3K36 per nucleosome was sufficient to silence cryptic transcription in vivo. We also demonstrate the utility of this system for analysis of histone modification crosstalk, using mass spectrometry to separately identify modifications on each H3 molecule within asymmetric nucleosomes. The ability to generate asymmetric nucleosomes in vivo and in vitro provides a powerful and generalizable tool to probe the mechanisms by which H3 tails are read out by effector proteins in the cell

Deep learning predicts function of live retinal pigment epithelium from quantitative microscopy.

Increases in the number of cell therapies in the preclinical and clinical phases have prompted the need for reliable and non-invasive assays to validate transplant function in clinical biomanufacturing. We developed a robust characterization methodology composed of quantitative bright-field absorbance microscopy (QBAM) and deep neural networks (DNNs) to non-invasively predict tissue function and cellular donor identity. The methodology was validated using clinical-grade induced pluripotent stem cell derived retinal pigment epithelial cells (iPSC-RPE). QBAM images of iPSC-RPE were used to train DNNs that predicted iPSC-RPE monolayer transepithelial resistance, predicted polarized vascular endothelial growth factor (VEGF) secretion, and matched iPSC-RPE monolayers to the stem cell donors. DNN predictions were supplemented with traditional machine learning algorithms that identified shape and texture features of single cells that were used to predict tissue function and iPSC donor identity. These results demonstrate non-invasive cell therapy characterization can be achieved with QBAM and machine learning

Designer cell signal processing circuits for biotechnology

Microorganisms are able to respond effectively to diverse signals from their environment and internal metabolism owing to their inherent sophisticated information processing capacity. A central aim of synthetic biology is to control and reprogramme the signal processing pathways within living cells so as to realise repurposed, beneficial applications ranging from disease diagnosis and environmental sensing to chemical bioproduction. To date most examples of synthetic biological signal processing have been built based on digital information flow, though analogue computing is being developed to cope with more complex operations and larger sets of variables. Great progress has been made in expanding the categories of characterised biological components that can be used for cellular signal manipulation, thereby allowing synthetic biologists to more rationally programme increasingly complex behaviours into living cells. Here we present a current overview of the components and strategies that exist for designer cell signal processing and decision making, discuss how these have been implemented in prototype systems for therapeutic, environmental, and industrial biotechnological applications, and examine emerging challenges in this promising field

Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats

Portal hypertension (PH) is a major cause of morbidity and mortality in chronic liver disease. Infection and inflammation play a role in potentiating PH and pro-inflammatory cytokines, including TNF, are associated with severity of PH. In this study, cirrhotic bile duct ligated (BDL) rats with PH were treated with Infliximab (IFX, a monoclonal antibody against TNF) and its impact on modulation of vascular tone was assessed. BDL rats had increased TNF and NFkB compared to sham operated rats, and their reduction by IFX was associated with a reduction in portal pressure. IFX treatment also reduced hepatic oxidative stress, and biochemical markers of hepatic inflammation and injury. IFX treatment was associated with an improvement in eNOS activity and increased L-arginine/ADMA ratio and DDAH1 expression. In vitro analysis of HepG2 hepatocytes showed that DDAH1 protein expression is reduced by oxidative stress, and this is in part mediated by post-transcriptional regulation by the 3′UTR. This study supports a role for the DDAH1/ADMA axis on the effect of inflammation and oxidative stress in PH and provides insight for new therapies

Therapeutic efficacy of artemether-lumefantrine in uncomplicated falciparum malaria in India

<p>Abstract</p> <p>Background</p> <p>Artemisinin-based combination therapy (ACT) is the treatment of choice for uncomplicated falciparum malaria. Artemether-lumefantrine (AL), a fixed dose co-formulation, has recently been approved for marketing in India, although it is not included in the National Drug Policy for treatment of malaria. Efficacy of short course regimen (4 × 4 tablets of 20 mg artemether plus 120 mg lumefantrine over 48 h) was demonstrated in India in the year 2000. However, low cure rates in Thailand and better plasma lumefantrine concentration profile with a six-dose regimen over three days, led to the recommendation of higher dose globally. This is the first report on the therapeutic efficacy of the six-dose regimen of AL in Indian uncomplicated falciparum malaria patients. The data generated will help in keeping the alternative ACT ready for use in the National Programme as and when required.</p> <p>Methods</p> <p>One hundred and twenty four subjects between two and fifty-five years of age living in two highly endemic areas of the country (Assam and Orissa) were enrolled for single arm, open label prospective study. The standard six-dose regimen of AL was administered over three days and was followed-up with clinical and parasitological evaluations over 28 days. Molecular markers <it>msp</it>-<it>1 </it>and <it>msp</it>-2 were used to differentiate the recrudescence and reinfection among the study subjects. In addition, polymorphism in <it>pfmdr</it>1 was also carried out in the samples obtained from patients before and after the treatment.</p> <p>Results</p> <p>The PCR corrected cure rates were high at both the sites viz. 100% (n = 53) in Assam and 98.6% (n = 71) in Orissa. The only treatment failure case on D7 was a malnourished child. The drug was well tolerated with no adverse events. Patients had pre-treatment carriage of wild type codons at positions 86 (41.7%, n = 91) and 184 (91.3%, n = 91) of <it>pfmdr1 </it>gene.</p> <p>Conclusion</p> <p>AL is safe and effective drug for the treatment of acute uncomplicated falciparum malaria in India. The polymorphism in <it>pfmdr</it>1 gene is not co-related with clinical outcome. However, treatment failure can also occur due to incomplete absorption of the drug as is suspected in one case of failure at D7 in the study. AL can be a viable alternative of artesunate plus sulphadoxine/pyrimethamine (AS + SP), however, the drug should be used rationally and efficacy needs to be monitored periodically.</p