A Caspase-activated Factor (CAF) Induces Mitochondrial Membrane Depolarization and Cytochrome c Release by a Nonproteolytic Mechanism

It is well established that apoptosis is accompanied by activation of procaspases and by mitochondrial changes, such as decrease in mitochondrial transmembrane potential (ΔΨm) and release of cytochrome c. We analyzed the causal relationship between activated caspases and these mitochondrial phenomena. Purified recombinant caspase-1, -11, -3, -6, -7, and -8 were incubated with mitochondria in the presence or absence of additional cellular components, after which ΔΨm was determined. At lower caspase concentrations, only caspase-8 was able to activate a cytosolic factor, termed caspase-activated factor (CAF), which resulted in decrease in ΔΨm and release of cytochrome c. Both CAF-mediated activities could not be blocked by protease inhibitors, including oligopeptide caspase inhibitors. CAF-induced cytochrome c release, but not decrease of ΔΨm, was blocked in mitochondria from cells overexpressing Bcl-2. CAF is apparently involved in decrease of ΔΨm and release of cytochrome c, whereas Bcl-2 only prevents the latter. Hence, CAF may form the link between death domain receptor–dependent activation of procaspase-8 and the mitochondrial events studied

The Release of Cytochrome c from Mitochondria during Apoptosis of NGF-deprived Sympathetic Neurons Is a Reversible Event

During apoptosis induced by various stimuli, cytochrome c is released from mitochondria into the cytosol where it participates in caspase activation. This process has been proposed to be an irreversible consequence of mitochondrial permeability transition pore opening, which leads to mitochondrial swelling and rupture of the outer mitochondrial membrane. Here we present data demonstrating that NGF-deprived sympathetic neurons protected from apoptosis by caspase inhibitors possess mitochondria which, though depleted of cytochrome c and reduced in size, remained structurally intact as viewed by electron microscopy. After re-exposure of neurons to NGF, mitochondria recovered their normal size and their cytochrome c content, by a process requiring de novo protein synthesis. Altogether, these data suggest that depletion of cytochrome c from mitochondria is a controlled process compatible with function recovery. The ability of sympathetic neurons to recover fully from trophic factor deprivation provided irreversible caspase inhibitors have been present during the insult period, has therapeutical implications for a number of acute neuropathologies

Deep convolutional neural network models for weed detection in polyhouse grown bell peppers

Conventional weed management approaches are inefficient and non-suitable for integration with smart agricultural machinery. Automatic identification and classification of weeds can play a vital role in weed management contributing to better crop yields. Intelligent and smart spot-spraying system's efficiency relies on the accuracy of the computer vision based detectors for autonomous weed control. In the present study, feasibility of deep learning based techniques (Alexnet, GoogLeNet, InceptionV3, Xception) were evaluated in weed identification from RGB images of bell pepper field. The models were trained with different values of epochs (10, 20,30), batch sizes (16, 32), and hyperparameters were tuned to get optimal performance. The overall accuracy of the selected models varied from 94.5 to 97.7%. Among the models, InceptionV3 exhibited superior performance at 30-epoch and 16-batch size with a 97.7% accuracy, 98.5% precision, and 97.8% recall. For this Inception3 model, the type 1 error was obtained as 1.4% and type II error was 0.9%. The effectiveness of the deep learning model presents a clear path towards integrating them with image-based herbicide applicators for precise weed management

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Not AvailableThe present study was carried out in a dry tract of central plateau region (Banwar village, Madhya Pradesh, India) by a team of trainee scientists inducted for the National Agricultural Research System (NARS), India. Observations recorded in the village indicated the harsh effects of declining water table owing to poor precipitation and the need for water harvesting to sustain people's livelihood since agriculture being their primary profession. Considering social marketing strategy, the goal was set to recharge groundwater and increase the water availability for irrigation purpose. Visualising groundwater recharge through water harvesting as the end product; organising training programmes, method demonstrations, village level exhibitions and visit to other successful water harvesting regions were set as the promotion strategies to achieve the set goal. Work plan was devised by the trainee scientists and set to act further. Nevertheless, successful social marketing needs the partnership of local government bodies and nongovernmental organizations (NGOs) that play a significant role in changing the complex social structure of the people—the ultimate beneficiaries of end product.Not Availabl

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Not AvailableINTRODUCTION: Brucellosis is one of the most important zoonotic diseases that affects multiple livestock species and causes great economic losses. The highly conserved genomes of Brucella, with > 90% homology among species, makes it important to study the genetic diversity circulating in the country. METHODOLOGY: A total of 26 Brucella spp. (4 reference strains and 22 field isolates) and 1 B. melitensis draft genome sequence from India (B. melitensis Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR) targeting bcsp 31Brucella genus-specific marker. Brucella speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST). RESULTS: The MLST typing of 27 Brucella spp. revealed five distinct sequence types (STs); the B. abortus S99 reference strain and 21 B. abortus field isolates belonged to ST1. On the other hand, the vaccine strain B. abortus S19 was genotyped as ST5. Similarly, B. melitensis 16M reference strain and one B. melitensis field isolate were grouped into ST7. Another B. melitensis field isolate belonged to ST8 (draft genome sequence from India), and only B. suis 1330 reference strain was found to be ST14. CONCLUSION: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis.Not Availabl

Suppressing mitochondrial respiration is critical for hypoxia tolerance in the fetal growth plate

Oxygen (O2) is both an indispensable metabolic substrate and a regulatory signal that controls the activity of Hypoxia-Inducible Factor 1\u3b1 (Hif1a), a mediator of the cellular adaptation to low O2 tension (hypoxia). Hypoxic cells require Hif1a to survive. Additionally, Hif1a is an inhibitor of mitochondrial respiration. Hence, we hypothesized that enhancing mitochondrial respiration is detrimental to the survival of hypoxic cells in vivo. We tested this hypothesis in the fetal growth plate, which is hypoxic. Our findings show that mitochondrial respiration is dispensable for survival of growth plate chondrocytes. Furthermore, its impairment prevents the extreme hypoxia and the massive chondrocyte death observed in growth plates lacking Hif1a. Consequently, augmenting mitochondrial respiration affects the survival of hypoxic chondrocytes by, at least in part, increasing intracellular hypoxia. We thus propose that partial suppression of mitochondrial respiration is crucial during development to protect the tissues that are physiologically hypoxic from lethal intracellular anoxia. The fetal growth plate is a hypoxic structure that gives rise to most of the skeleton. It is formed by cells known as chondrocytes. Yao et al. now show that impairment of mitochondrial respiration and, thus, oxygen consumption are crucial for the survival of hypoxic chondrocytes during fetal development